atgccgcactcgagggagaa

gaacttttcacgggagttgtcccaattcttgttgaattagatggtgatgttaatgggcac
aaattttctgtccgtggagagggtgaaggtgatgctacaaacggaaaactcacccttaaa
tttatttgcactactggaaaactacctgttccatggccaacacttgtcactactctgacc
tatggtgttcaatgcttttcccgttatccggatcacatgaaacggcatgactttttcaag
agtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggacc
tacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagtta
aagggtattgattttaaagaagatggaaacattcttggacacaaactcgagtacaacttt
aactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttc
aaaattcgccacaacgttgaagatggttccgttcaactagcagaccattatcaacaaaat
actccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatct
gtcctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaact
gctgctgggattacacatggcatggatgagctctacaaa
Primer Design Report: One Primer
Report Date: 06/18/13
Source molecule: PDAN5-~1.FAS

----- P R I M E R
Sequence:

5260 bps

I N F O --------------------------------------------

5'-GACCATGATTACGCCAAGC-3'
Length: 19;
5' Extension:
Composition:

Bp pos:
49
--none-A: 6 C: 6 G: 4 T: 3

---------------------------------------------------------------------------

PRIMER EVALUATION AND CRITERIA SETTINGS:

Criteria
-------------

Criteria Setting
----------------------------

Actual
------

% GC

Min

50,

Max

60

52

YES

Tm C

Min

55,

Max

80

69

YES

No Hairpins

Energy cutoff 0.0 kcal

--

YES

No 3' Dimers
No Dimers

Reject >= 3 matches at 3' end

Reject >= 7 adj homol bases

2
4

YES
YES

No Runs
No 3'GC runs

Reject >= 3 base runs

Reject >= 3 G or C at 3' end

2
2

YES
YES

LARGEST DUPLEXES REPORTED IN PRIMER DIMERS (above)

Primer-dimers with 3'end involvement:
5'-GACCATGATTACGCCAAGC-3'
::
3'-CGAACCGCATTAGTACCAG-5'

Meets Criteria
--------------

Other Primer-Dimers:
5'-GACCATGATTACGCCAAGC-3'
::::
3'-CGAACCGCATTAGTACCAG-5'