XC NH AMONI TRONG NC BNG PHNG PHP PHENAT

Theo SMEWW 4500-NH3 F :2012

1. Mc ch v phm vi p dng
- Vn bn ny hng dn phng php xc nh amoni trong mu nc ngt v
nc bin.
- Vn bn ny p dng cho cc th nghim vin thuc Phng Quan trc - Th
nghim ti Trung tm Quan trc v K thut Mi trng Qung Tr
2. Ti liu vin dn
- Quy trnh kim sot ti liu, m hiu: QT.01/QTMTQT
- Quy trnh kim sot h s, m hiu: QT.02/ QTMTQT.
- Quy trnh nhn mu v tr kt qu, m hiu: QT.08/QTMTQT
- Quy trnh ly mu v qun l mu, m hiu: QT.10/ QTMTQT.
- Quy trnh qun l trang thit b, m hiu: QT.13/ QTMTQT.
- Quy trnh qun l phng php th, m hiu: QT.14/ QTMTQT.
- Quy trnh tin hnh php th, m hiu: QT.15/QTMTQT
- Quy nh qun l s dng ha cht, dng c: Q.05/QTMTQT
- SMEWW 4500-NH3 F:2012
3. Nguyn tc
Da trn vic o mu bc sng 640 nm ca hp cht mu xanh, indophenol,
c to thnh gia amoni, hypoclorit v phenol. Phn ng c xc tc bi natri
nitroprusit. Phng php phenat c p dng xc nh amoni trong c nc ngt v
nc bin.
4. Yu t nh hng
- Ca2+, Mg2+ nh hng n php xc nh do hnh thnh kt ca hidroxyt trong mi
trng kim, loi tr bng citrat.
- c: loi tr bng cch chng ct hay lc mu
- H2S: loi tr bng cch axit ha n pH < 3 vi HCl
5. Bo qun mu
S dng mt trong 3 cch bo qun sau:
Lm lnh mu 40C v khng axit ha: mu n nh trong 24 gi
Lm lnh mu 40C v axit ha n pH < 2: mu n nh trong 28 ngy
Lm lnh mu -200C v khng axit ha: mu n nh trong 28 ngy
(Lu : mu bo qun bng axit phi trung ha bng KOH hoc NaOH trc khi xc
nh)
6. Thit b, dng c v ha cht

6.1. Thit b, dng c

- My trc quang s dng bc sng 640 nm, cuvet c b dy 1 cm
- Cc dng c thy tinh pha ch v bo qun thuc th...
6.2. Ha cht
Dung dch phenol: trn 11,1 ml phenol ( 89%) vi ru etylic (95%) n 100 ml.
Pha hng tun.
(lu : s dng gng tay v knh bo v mt trnh phenol bn vo mt v da
gy bng, hn ch ht hi c phenol bng cch eo khu trang v lm th nghim
trong iu kin thng kh tt)
Dung dch natri nitroprusit: ho tan 0,5 g natri nitroprusit trong 100 ml nc ct.
Bo qun trong chai mu h phch hay nu. Dung dch bn trong 1 thng.
Dung dch citrat: ho tan 200 g natri citrat v 10 g NaOH trong nc ct v pha
long n 1000 ml.
Dung dch natri hypoclorit (NaOCl): s dng thuc ty thng mi 5%. Dung dch
bn trong 2 thng.
Dung dch oxi ho: trn 100 ml dung dch citrat vi 25 ml dung dch natri
hypoclorit. Chun b dung dch hng ngy.
Dung dch gc amoni: ha tan 3,819 g NH4Cl khan ( sy kh 1000C) trong
nc v pha long n 1000 ml. 1,00 ml dung dch ny cha 1 mg N (nng
1000 mg N-NH3/l).
Dung dch amoni trung gian 1: 10,0 ml dung dch amoni gc pha long n 100
ml. 1,00 ml dung dch ny cha 0,1 mg N (nng 100 mg N-NH3/l).
Dung dch amoni trung gian 2: 10,0 ml dung dch amoni trung gian 1 pha long
n 100 ml. 1,00 ml dung dch ny cha 0,01 mg N (nng 10 mg N-NH3/l).
Dung dch amoni lm vic: 10 ml dung dch chun amoni trung gian nh mc
50ml. 1,00 ml dung dch ny cha 0,002 mg N (nng 2 mg N-NH3/l).
7. Quy trnh phn tch
Ly 25,0 ml mu cho vo bnh nh mc dung tch 50 ml. Thm 1 ml dung
dch phenol, 1 ml dung dch natri nitroprusit, 2,5 ml dung dch oxi ho (trn ln u
dung dch sau mi ln thm thuc th), thm nc ct n vch mc v trn u.
trong bng ti, nhit phng (22 270C) t nht 1 gi. o mt quang bc
sng 640 nm vi dung dch mu trng lm dung dch so snh. Mu n nh trong 24
gi.
7.1. Xy dng ng chun:
Pha dy dung dch chun c nng t 0,02 0,25 mg N-NH3/l theo bng sau:
STT
Th tch dd amoni lm vic (ml)
Th tch dd phenol
(ml)

1
1
1

2
3
1

3
5
1

4
7
1

5
8
1

6
10
1

Th tch dd natri nitroprusit (ml)

(ml)

2,5

2,5

(ml)
(mg N-NH3/l)

0,04

0,12

Th tch dd oxi ho
Th tch nc ct
Nng amoni

2,5
2,5
Va 50 ml

2,5

2,5

0,20

0,32

0,40

0,28

Cch tin hnh xc nh amoni trong mu chun nh quy trnh trn

Mu trng: tng t nh mu chun nhng thay dung dch lm vic bng nc ct
Xy dng phng trnh ng chun A = f(CN-NH3)
Nng amoni trong mu c xc nh da vo phng trnh ng chun
7.2. Tnh ton kt qu
Nhu cu oxi ha hc c xc nh thng qua phng trnh ng chun
NH3-N[mg/l] = Ci; Trong : Ci: Nng ca my c c
8. Kt thc th nghim
- Ghi qu trnh v kt qu thc hin vo s theo di.
- Lp phiu kt qu th nghim.
- V sinh dng c, thit b v khu vc th nghim.
9. H s cng vic
H s theo di phng php xc nh hm lng amoni gm:
TT

Tn gi h s

1 S tay th nghim vin

2

Phiu bo kt qu th
nghim

M hiu

Ni lp Ni lu Thi gian lu

PTN

BM.02/QT.15/QTMTQT

PTN

PTN

3 nm

PTN

3 nm

Cc h s ny c c nhn, n v lin quan lp v kim sot theo qui nh ti

Quy trnh kim sot h s, m hiu: QT.02/QTMTQT.