Total Protein Assays

Micro BCA™ Protein Assay

Most sensitive BCA™ Formulation measuring dilute
protein solutions from 0.5 to 20 μg/ml. Ordering Information
Pkg. U.S.
Highlights: Product # Description Size Price
23235 Micro BCA™ Protein Assay Kit Kit $159
• Colorimetric method; read at 562 nm Sufficient reagents to perform 480 standard
• Compatible with most ionic and nonionic detergents tube assays or 3,200 microplate assays.
Includes: Micro Reagent A (MA) (Sodium 240 ml
• A very sensitive reagent for dilute protein samples carbonate, sodium bicarbonate,
• Linear working range for BSA: 0.5-20 μg/ml and sodium tartrate in 0.2 N NaOH)
Micro Reagent B (MB) 240 ml
• Less protein:protein variation than dye-binding methods (4% BCA in water)
• All kit reagents stable at room temperature for two years Micro Reagent C (MC) (4% cupric 12 ml
sulfate pentahydrate in water
• Working reagent is stable for 24 hours Albumin Standard Ampules (2 mg/ml)
• Convenient microplate or cuvette format 23231 Micro BCA™ Reagent A (MA) 240 ml $ 39
23232 Micro BCA™ Reagent B (MB) 240 ml $ 74
References
Smith, P.K., et al. (1985). Anal. Biochem. 150 (1), 76-85. 23234 Micro BCA™ Reagent C (MC) 12 ml $ 24
Kang, D.E., et al. (2002). Cell 110, 751-762.
23209 Albumin Standard Ampules, 2 mg/ml 10 x 1 ml $ 36
Rawadi, G., et al. (1999). J. Immunol. 162, 2193-2203.
Blum, D., et al. (2002). J. Neurosci. 22, 9122-9133.
Contains: Bovine Albumin Fraction V in 0.9%
Paratcha, G., et al. (2003). Cell 113, 867-879. NaCl solution containing sodium azide

25 parts “MA” 1.0 ml sample

24 parts “MB” + 1.0 ml working reagent Incubate: 60 minutes at 60˚C Spectrophotometer
18 1 parts “MC”

Mix working reagent Mix well Then cool Read at 562 nm

Micro BCA™ Protein Assay protocol.

For more product information, or to download a product instruction booklet, visit www.piercenet.com/path95n.