http://wiki.bugwood.

org/SX_agar

SX agar
Tabla de contenidos
[ocultar]
1 Purpose
2 Ingredients
3 Instructions
4 Notes
5 References
6 Contributed by
Purpose
Semiselective medium for isolation of starch-positive pathovars of
Xanthomonas campestris (X. c. pvs. campestris, begoniae, fragaria, and
corylina) from soil or plant tissues. Also useful for direct plating of contaminated
seeds.
Ingredients
Ingredient 1 L 500 ml
Distilled water 1000 ml 500 ml
Soluble potato starch 10 g 5 g
Beef extract 1 g 0.5 g
Ammonium chloride 5 g 2.5 g
K
2
HPO
4
1
2 g 1 g
Agar 15 g 7.5 g
Methyl violet 2B (1% solution in 20% ethanol) 1 ml 0.5 ml
Methyl green (1% solution) 2 ml 1 ml
Cycloheximide (optional)* 0.25 g 0.125 g
Instructions
Notes
The medium should be violet in color when
melted. Fahy (Plant Bacterial Diseases)
incorrectly lists the wrong potassium salt, which
results in a blue slush medium. It is also incorrect
in the original paper
1
. The above, K2HPO4 is
correct.

Cycloheximide can be added to inhibit fungi from
growing on the medium.
References
Schaad, N. W., Jones, J. B. and Chun, W. 2001.
Laboratory guide for the identification of plant
pathogenic bacteria, 3rd edition. The American
Phytopathological Society, St. Paul, MN. 373pp.