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5C18-MS-II Silica Gel Average Particle Size Average Pore Size Stationary Phase / Bonding Type Monomeric Type
5C18-AR-II High Purity Porous Spherical Silica 3, 5, 15 m approx. 120 Polymeric Type
5C18-PAQ
H3C
Si
CH3
Si
OH
COSMOSIL 5C18-MS-II
COSMOSIL 5C18-MS-II is a monomeric type of C18 phase. A new end capping treatment with polar groups for "shield effect" has extended the pH range and signicantly improved peak shape for basic compounds. This phase is recommended for most of applications but particularly effective for low molecular weight organic compounds.
COSMOSIL 5C18-AR-II COSMOSIL 5C18-AR-II is a polymeric type of C18 phase. It shows exceptional stability and long lifetime at low pH. This phase is recommended for the separations requiring acidic mobile phase conditions. It also shows superior molecular shape selectivity to monomeric type C18 columns. COSMOSIL 5C18-PAQ
COSMOSIL 5C18-PAQ is designed to offer superior retention of polar compounds and excellent reproducibility in highly aqueous mobile phases, even in 100% aqueous.
: MS-II shows strongest durability. In some cases, AR-II is not recommended because the tailing may be occurred. Basic Compounds Metal Coordination Compounds : There is not so much difference between three ODS. So the most multi-purpose MS-II is recommendable. Acidic Buffer as Mobile Phase : AR-II is the strongest acid resistible column in our ODS. Also, MS-II and PAQ can be used in acidic condition. : PAQ is able to be stabilized the retention time. MS-II and AR-II can be used, but the stability is not good. Hydrophilic Compounds (especially used 100% water as mobile phase)
Application Data
COSMOSIL 5C18-MS-II Basic Compounds
Procainamide Benzylamine Pyridine
Column Size 4.6 mm I.D. x 150 mm Mobile Phase Methanol 20 mmol/l Phosphate Buffer (pH7) = 20 : 80 Flow Rate 1.0 ml/min Temperature 30 Detection UV 254 nm Sample 1. Procainamide 2. N-Acetylprocainamide 3. Benzyl Alcohol (0.38 g) (0.25 g) (5.63 g)
Column Size 4.6 mm I.D. x 150 mm Mobile Phase Methanol : 20 mmol/l Phosphate Buffer (pH3) = 30 : 70 Flow Rate 1.0 ml/min Temperature 30 Detection UV 254 nm Sample 1. Uracil 2. Benzylamine 3. N,N-Dimethylbenzylamine 4. Phenol
Column Size 4.6 mm I.D. x 250 mm Mobile Phase Acetonitrile : 50mmol/l (NH4)H2PO4 = 30 : 70(pH7 with NH3) Flow Rate 1.0 ml/min Temperature 40 Detection UV 254 nm Sample 1. Ceftazidime (0.5 g) (0.5 g) 2. Pyridine
5C18-AR-II
5C18-MS-II
4.6 mm I.D. x 150 mm Methanol : Water = 80 : 20 1.0 ml/min 30 UV 254 nm 1.0 AUFS 1. o-Terphenyl 2. Triphenylene
1. o-Terphenyl
2. Triphenylene
Acidic Compounds
Organic acids Paraben Salicylic acids
Column Size 4.6 mm I.D. x 150 mm Mobile Phase Methanol : 20 mmol/l Phosphoric Acid = 20 : 80 Flow Rate 1.0 ml/min Temperature 30 Detection UV 254 nm 0.5 AUFS Sample 1. Gallic Acid 2. Protocatechuic Acid 3. Gentisic Acid 4. Phthalic Acid (0.63 g) (0.63 g) (0.63 g) (0.63 g)
4.6 mm I.D. x 150 mm Acetonitrile : Water = 50 : 50 1.0 ml/min 30 UV 254 nm 0.12AUFS 1. Methyl p-Hydroxybenzoate 2. Ethyl p-Hydroxybenzoate 3. Propyl p-Hydroxybenzoate 4. Butyl p-Hydroxybenzoate (0.125 g) (0.125 g) (0.125 g) (0.125 g)
4.6 mm I.D. x 150 mm Acetonitrile : Water = 70 : 30 1.0 ml/min 30 UV 254 nm 0.2 AUFS 1. Methyl Salicylate 2. Ethyl Salicylate 3. Propyl Salicylate 4. Butyl Salicylate (2.3 g) (2.6 g) (2.3 g) (2.6 g)
4.6 mm I.D. x 150 mm Acetonitrile : 2%Acetic Acid = 50 : 50 1.0 ml/min 30 UV 289 nm 1. Hinokitol 2. Magnolol (1 g) (1 g)
4.6 mm I.D. x 150 mm Acetonitrile : 20 mmol/l Phosphoric Acid = 5:95 1.0 ml/min 30 UV 240 nm 0.2 AUFS 1. Oxine-cooper
4.6 mm I.D. x 150 mm Acetonitrile : 20 mmol/l Phosphate Buffer(pH7) = 60 : 40 1.0 ml/min 30 UV 240nm 0.2 AUFS 1. Acetylacetone (0.76 g) 2. 8-Quinolinol (0.70 g) 3. Benzene (14.65 g)
4.6 mm I.D. x 250 mm 20mmol/l Phosphate Buffer (pH2.5) 1.0 ml/min 30 UV 210 nm (6.3 g) 1. Glycolic Acid (4.0 g) 2. Tartaric Acid (6.4 g) 3. Malic Acid (13 g) 4. Lactic Acid (13 g) 5. Acetic Acid (6.3 g) 6. Citric Acid (13 g) 7. Succinic Acid (0.06 g) 8. Maleic Acid (13 g) 9. Propionic Acid (0.03 g) 10. Fumaric Acid
4.6 mm I.D. x 150 mm 20 mmol/l Phosphate Buffer (pH7) 1.0 ml/min 30 UV 260 nm 1. Cytosine (0.16 g) 2. Uracil (0.16 g) 3. Cytidine (0.32 g) 4. Uridine (0.24 g) 5. Guanine (0.16 g) 6. Thymine (0.16 g) 7. Adenine (0.08 g) 8. Guanosine (0.24 g) 9. Thymidine (0.24 g) 10.Adenosine (0.16 g)
4.6 mm I.D. x 250 mm 20 mmol/l Phosphate Buffer (pH7) 1.0 ml/min 30 UV 254 nm 0.16 AUFS 1. dCTP (0.1 l) 2. dTTP (0.1 l) 3. dGTP (0.1 l) 4. dATP (0.2 l) (1 mmol/l each)
2-Phenylethyl Glucosides
3 1 2 4 5
10
20
30 min
4.6 mm I.D. x 250 mm 20 mmol/l Phosphoric Acid 1.0 ml/min 30 UV 245 nm 0.16 AUFS 1. Ascorbic Acid (0.1 g) (Vitamin C)
Column COSMOSIL PAQ Column Size 4.6 mm I.D. x 150 mm Mobile Phase Acetonitrile : Methanol : Water = 8 : 4 : 88 Flow Rate 1.0 ml/min Temperature 30 Detection UV 210 nm 0.064 AUFS Pressure 10.5 MPa
Sample 1. 2-Phenylethyl -melibioside 2. 2-Phenylethyl -gentiobioside 3. 2-Phenylethyl -lactoside 4. 2-Phenylethyl -cellobioside 5. 2-Phenylethyl -maltoside
Ordering Information
Particle Size: 5 m (Analytical Columns)
Column Size Packed Column 1.0 mm I.D x 50 mm 1.0 mm I.D x 150 mm 2.0 mm I.D x 30 mm 2.0 mm I.D x 50 mm 2.0 mm I.D x 100 mm 2.0 mm I.D x 150 mm 2.0 mm I.D x 250 mm 3.0 mm I.D x 100 mm 3.0 mm I.D x 150 mm 3.0 mm I.D x 250 mm 4.6 mm I.D x 30 mm 4.6 mm I.D x 50 mm 4.6 mm I.D x 100 mm 4.6 mm I.D x 150 mm 4.6 mm I.D x 150 mm 3 lots set 4.6 mm I.D x 250 mm 6.0 mm I.D x 150 mm 6.0 mm I.D x 250 mm 10.0 mm I.D x 50 mm 10.0 mm I.D x 150 mm 10.0 mm I.D x 250 mm 20.0 mm I.D x 150 mm 20.0 mm I.D x 250 mm 28.0 mm I.D x 250 mm Guard Column 4.6 mm I.D x 10 mm 4.6 mm I.D x 10 mm Cartridge* 10.0 mm I.D x 20 mm 20.0 mm I.D x 20 mm 20.0 mm I.D x 50 mm 28.0 mm I.D x 50 mm COSMOSIL 5C18-MS-II Product Number 02824-31 02896-01 05876-71 04355-21 05597-31 38025-91 05761-61 05458-51 34245-31 34254-11 34341-61 38017-01 38018-91 38019-81 09397-73 38020-41 38021-31 38022-21 05789-21 34355-91 38023-11 05091-41 38024-01 05760-71 38014-31 38015-89 38016-11 05790-81 34371-71 34347-01 COSMOSIL 5C18-AR-II Product Number 02955-21 02951-61 05098-71 34400-81 34469-11 37992-51 05272-71 05791-71 38028-61 38029-51 05877-61 38142-51 38143-41 38144-31 09396-83 38145-21 38146-11 38147-01 05369-21 34350-41 38149-81 34316-01 38150-41 34362-91 38141-61 38008-89 38148-91 34458-51 34479-81 34363-81 COSMOSIL 5C18-PAQ Product Number 05792-61 05793-51 05878-51 05794-41 05470-71 34449-71 05795-31 05796-21 05797-11 05798-01 05879-41 34451-21 05799-91 02486-71 02485-81 34419-61 05800-41 05801-31 34466-41 34376-21 34476-11 34373-51 34456-71 02484-91 34457-61 05803-11 05804-01 34455-81
Guard Column
For research use only, not intended for diagnostic or drug use.
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