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Name: Ori Baronin Exercise 3: Neurophysiology of Nerve Impulses: Activity 9: The Action Potential: Putting It All Together Lab

Report Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. 1. Sensory neurons respond to an appropriate sensory stimulus with a change in membrane potential that is You correctly answered: b. graded with the stimulus intensity. 2. If the depolarization that reaches the axon is large and suprathreshold, the result in the axon is You correctly answered: c. action potentials at higher frequency. 3. At the axon terminal, each action potential causes the release of neurotransmitter. This neurotransmitter diffuses to the receiving end of an interneuron, where it binds to receptors and causes You correctly answered: a. ion channels to open, so that the receiving end of the interneuron depolarizes. 4. Interneurons respond to chemical (neurotransmitter) stimulation with a change in membrane potential that is You correctly answered: b. graded with the stimulus intensity.

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Experiment Results Predict Question: Predict Question 1: If you apply a very weak, subthreshold stimulus to the sensory receptor Your answer : d. no response will occur at any of the four locations. Predict Question 2: If you apply a moderate stimulus to the sensory receptor Your answer : d. small, depolarizing responses will occur at all four locations. Predict Question 3: If you apply a strong stimulus to the sensory receptor Your answer : c. action potentials will be generated at all 4 locations Stop & Think Questions: Observe the two neurons in the petri dish. What do you think is the resting membrane potential at the receiving end of the sensory neuron (sensory receptor) and the receiving end of the interneuron? You correctly answered: c. -70 mV Why is there no response at R3 when you apply a very weak stimulus to the sensory receptor? You correctly answered: c. The very weak stimulus does not depolarize the axon of the sensory neuron to threshold. Why is there a larger, depolarizing response at R1 when you apply a moderate intensity stimulus? You correctly answered: c. The stimulus induces a graded receptor potential at R1. Experiment Data: Stimulus Sensory Neuron Membrane Potential (mV) Receptor -70 -60 -40 -25 Sensory Neuron AP Frequency (Hz) in Axon 0 16.6 33.3 Sensory Neuron Vesicles Released from Axon Terminal 0 4 6 Interneuron Membrane Potential (mV) Receiving End -70 -70 -50 -40 Interneuron AP Frequency (Hz) in Axon 0 5 10

None Weak Moderate Strong

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Post-lab Quiz Results You scored 100% by answering 5 out of 5 questions correctly. 1. What determines the amplitude of the depolarization at the sensory receptor (R1)? You correctly answered: a. The strength of the stimulus applied to the sensory receptor. 2. What determines the frequency of action potentials in the axon of the sensory neuron (R2)? You correctly answered: a. The amplitude of the depolarization at the sensory receptor (R1). 3. Which of the following directly determines the amount of neurotransmitter released at the axon terminal of the sensory neuron? You correctly answered: c. The amount of calcium that enters the sensory receptor. 4. Which of the following directly or indirectly determines the amount of neurotransmitter released at the axon terminal of the sensory neuron? You correctly answered: d. All of the above play a role in determining the amount of neurotransmitter released. 5. Which of the following directly or indirectly determines the frequency of action potentials in the axon of the interneuron? You correctly answered: d. All of the above play a role in determining the frequency of action potentials in the axon of the interneuron.

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Review Sheet Results You have not completed the Review Sheet.

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