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  • Lambda DNA EcoRI HindIII Digest

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    adnil1402
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    EcoR I and Hind III Digest of l DNA yields 13 discrete fragments (in base pairs) 21226, 5148, 4973, 4268, 3530, 2027, 1904, 1584, 1375, 947, 831, 564, 125. DNA may denature if diluted in dH2O and subsequently heated.

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    EcoR I and Hind III Digest of l DNA yields 13 discrete fragments (in base pairs) 21226, 5148, 4973, 4268, 3530, 2027, 1904, 1584, 1375, 947, 831, 564, 125. DNA may denature if diluted in dH2O and subsequently heated.

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    18 vistas1 página

    Lambda DNA EcoRI HindIII Digest

    Cargado por

    adnil1402
    EcoR I and Hind III Digest of l DNA yields 13 discrete fragments (in base pairs) 21226, 5148, 4973, 4268, 3530, 2027, 1904, 1584, 1375, 947, 831, 564, 125. DNA may denature if diluted in dH2O and subsequently heated.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Descargue como PDF, TXT o lea en línea desde Scribd
    Marcar por contenido inapropiado
    de 1

    Lambda DNA/EcoR I/Hind III Digest

    Concentration: 0.5g/l
    bp

    Description: The EcoR I and Hind III digest of DNA yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125. Preparation: Lambda DNA was completely digested by EcoR I and Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Storage buffer: 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Store at -20oC. Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated. *The cohesive ends (12b cos site of bacteriophage ) of fragments 21226bp and 3530bp may anneal to and form the additional band. These fragments may be separated by heating to 65oC for 5 min and then cooling on ice for 3min. Related products: 10X Loading Dye Solution
    1.0% agarose

    21226*

    5148 4973 4268 3530*

    2027 1904 1584 1375

    947 831

    564

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