PREPARACION DE LA CURVA DE CALIBR

REPARACION DE LA CURVA DE CALIBRACION

CURVA DE CALIBR

A540

0.35

0
0.04
0.1
0.17
0.24
0.31

ABSORBANCIA A 540 NM

0
0.2
0.4
0.6
0.8
1

0.3
0.25
0.2
0.15
0.1
0.05
0
-0.05 0

0.2

0.4

CONCENTRACION M

COEF. DE EXTINCION=0 .3131

A=EBC
C=A/BE

OBTENCION DE K (orden 1)
1.6
1.4
1.2

centracin

GLUCOSA conc

concentracin

1.2
1
0.8
0.6
0.4
0.2
0
0

10

15

20
tiempo

CURVA DE CALIBRADO

0.6
CONCENTRACION MG/ML

y = 0.3171x - 0.0152
R = 0.9926

0.8

1.2

CION=0 .3131

B=
E=

1
0.3131

TIEMPO

K (orden 1)

ABSORBANCIA
0
5
10
15
20
25
30

y = 0.0433x + 0.0869
R = 0.9786

0.25
0.35
0.48
0.51
0.62
0.8
1

CONCENTRACION
0.798466943
1.117853721
1.533056531
1.628872565
1.98019802
2.555094219
3.193867774

LN C/CO
0
0.33647224
0.65232519
0.71294981
0.90825856
1.16315081
1.38629436

25

30

35