Fed-Batch Fermentation: A Practical Guide to Scalable Recombinant Protein Production in Escherichia Coli
By G G Moulton
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About this ebook
- This process can be used as a teaching tool for the inexperienced fermentation student or researcher in the fields of bioprocessing and bioreactors. It is an important segue from E. coli shake flask cultures to bioreactor
- The fed-batch fermentation is designed to be accomplished in a single day with the preparation work being done on the day prior
- The fed-batch fermentation described in this book is a robust process and can be easily scaled for CMO production of protein product
G G Moulton
Garner G. Moulton has a Masters of Science in Biology from the Program of Cell and Molecular Biology at San Diego State University. He has been working in biotech and non-profit research for over 25 years, and has focussed specifically on protein purification and fermentation process development over the last 17 years. During the past 4 years he has been employed by the Infectious Disease Research Institute (IDRI) in Seattle, WA, USA, where he is a principal research associate in charge of the Process Development Department. He has developed many robust fermentation processes that can be adapted to many different recombinant products without having to re-optimize conditions of growth and expression. Numerous projects have been successfully transferred to a contract manufacturer for scale-up production and use in clinical trials.
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