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INSTITUTION: ___Indiana University-Purdue University Indianapolis (IUPUI)_____________ COLLEGE: ____School of Science ___________________________________________ DEPARTMENT:__Biology____________________________________________________ DEGREE PROGRAM TITLE:__Doctor of Philosophy in Biology____________________________ FORM OF RECOGNITION TO BE AWARDED/DEGREE CODE: _____Ph.D.______ SUGGESTED CIP CODE:____________________________________________________ LOCATION OF PROGRAM/CAMPUS CODE: __Indianapolis_________________ PROJECTED DATE OF IMPLEMENTATION: ___8/11________________ DATE PROPOSAL WAS APPROVED BY INSTITUTIONAL BOARD OF TRUSTEES: ____________________________________

_____________________________________ SIGNATURE OF AUTHORIZING INSTITUTIONAL OFFICER _____________________________________ DATE ______________________________________ DATE RECEIVED BY COMMISSION FOR HIGHER EDUCATION

_____________________________________ COMMISSION ACTION (DATE)

A. ABSTRACT Doctor of Philosophy (Ph.D.) in Biology To be offered by Indiana University Purdue University Indianapolis (IUPUI) on the IUPUI campus in Indianapolis Objectives: The prime objective of this proposal is to convert an existing Purdue Ph.D. program in Biological Sciences, which is entirely delivered on the Indianapolis campus but is currently credited to Purdue West Lafayette, to a Purdue Ph.D. program in Biology that is site-approved for Indianapolis (IUPUI). The IUPUI Department of Biology has been training Ph.D. students for twenty years with eighteen out of twenty-three graduates earning the Purdue University Ph.D. in Biological Sciences and with the vast majority going on to successful careers in science. A second objective of the proposed degree is to train researchers able to contribute to their fields and to contribute to the economic development of Indiana and beyond. A final objective for this research-focused Purdue Ph.D. program in Biology at IUPUI is to empower our expanded research faculty to drive increased research collaboration across campus in order to make IUPUI more competitive for external funding. Clientele to be Served: The program will serve highly qualified, research-motivated baccalaureate and masters degree holders from Indiana and beyond. It will be primarily for full-time students although special cases may be made for students holding employment in local scientific positions who can satisfy the faculty that their employment obligations will permit timely completion of the doctoral degree requirements. Curriculum: As is the case with the Purdue West Lafayette Ph.D. program in Biological Sciences through which we have been offering the Ph.D., the proposed Purdue Indianapolis Ph.D. program envisions no requirements for specific course work that would apply to all students (core courses). The program will require the traditional 90 credit hours of registration and the Graduate Advisory Committee of each student will determine the course work component. This customization of the curriculum is necessary to accommodate a range of student backgrounds and the diversity of the research opportunities available in the program. Students typically enroll in about 24 credit hours of course work. In keeping with the Purdue West Lafayette Ph.D. program, students will be required to present 2 one-hour seminars on topics unrelated to their research. These experiences will usually appear (unless satisfied in another way) as two separate credit hours of BIOL 69600, Seminar, on transcripts and Plans of Study. Most of the students in the program will come with undergraduate and/or masters degrees in Biology. Because our program is broad, we are also seeking talented and highly motivated students from other fields. Thus, those with degrees in related areas

(Biochemistry, Chemistry) and those in other programs who have basic preparation in chemistry, physics and the biological sciences would be welcome applicants. Employment Possibilities: Our past students have gone on to postdoctoral appointments at recognized universities and subsequently have taken permanent positions at universities or in government and industrial laboratories (see section C3). Other graduates have gone directly into industry. Several of these placements have been in Indiana but Ph.D. placement cannot be expected to place graduates in local employment to a significant extent. B. PROGRAM DESCRIPTION 1. Program description and objectives. The program is structured similarly to most Ph.D. programs in Biology in that it is research intensive and requires 90 credit hours of registration. It will prepare students for post-doctoral study, positions in government and industry and appointments to college and university faculty positions. The prime objective of this proposal is to create an Indianapolis-based, Purdue Ph.D. program in Biology at IUPUI, an Urban Research University that has been designated by the Indiana University System as its Health & Life Sciences campus. The Department of Biology is a key contributor to the health emphasis on campus by providing foundation course work for undergraduate health programs, undergraduate degree programs that feed into professional programs and health-related graduate programs designed to prepare students for health profession training programs on campus. The department is also the setting for growing externally funded research programs with strengths in a variety of fields with an emphasis in basic biomedical research. The Ph.D. program will offer training in the highest degree offered by institutions of higher education and will complement the advanced degree programs in the professional schools on campus. The Department of Biology has been training Ph.D. students for twenty years with the majority earning the Purdue University Ph.D. in Biological Sciences, a degree awarded on the West Lafayette campus despite the fact the 100% of the course work and research is completed at IUPUI. The Department of Biology has seen increased interest in it programs over the last few years and is in the midst of a hiring initiative that will bring additional research-focused faculty members on board. Having a Purdue Ph.D. program that awards degrees in Indianapolis as we undergo expansion will allow for increased research collaboration across campus, will make IUPUI more competitive for external funding, and will allow IUPUI to retain rightful ownership of the degrees awarded. The research focus of IUPUI has generated economic benefits for central Indiana in terms of expenditures from external funding on goods and services as well as the employment of highly educated professionals. While most of this impact is attributed to the IU School of Medicine, the Department of Biology has seen grant success rise to the point where there are now fifteen to twenty full time individuals (post-doctoral appointees, technicians, Ph.D. students, M.S. thesis students) supported on external

funding. In addition, there are many hourly individuals who derive their wages from these funds. Thus, the Biology research programs are contributing at increasingly higher levels to the transformation of the Indiana economy and its emphasis to develop the life sciences. The availability of Ph.D. students is essential in the recruiting of high caliber research faculty and in the completion the work contracted for in funded proposals. The program proposed will be structured much like the Purdue West Lafayette Ph.D. program in the Department of Biological Sciences, the one in which we have been participating. This program is characterized by an extensive research element that is closely monitored by a Graduate Advisory Committee. Our primary academic objective is to produce Ph.D. graduates who are trained in the latest technologies and techniques, who have the foundational skills to direct an independent program of research and who are competitive for positions in academia, industry and government. 2. Admission requirements, student clientele, and student financial support All applicants must have earned, at the minimum, a four-year baccalaureate degree from a U.S. institution or an equivalent degree from a foreign institution. For admission, applicants must complete the on-line application, submit original transcripts from all institutions previously attended, submit a set of scores from the general GRE, and arrange for letters of recommendation from three individuals who can evaluate the applicants past work and comment on the applicants potential for successful work at the graduate level. International applicants must also submit TOEFL scores unless their undergraduate degrees were earned from U.S. institutions or select international institutions where instruction was delivered exclusively in English. Applicants will be expected to have completed basic course work in Biology, Chemistry, Physics, and Mathematics for admission. Because the curriculum (see section B.3 below) is built for maximum flexibility, deficiencies can be addressed while in the program. The primary traits we seek in applicants are ability and motivation. We can adjust for academic background within the program. Completed applications will be reviewed by an Admissions Committee comprised of graduate faculty members from the Department of Biology. The Committee will screen for competitive GRE scores, minimum TOEFL scores (Paper 600, Computer 250, and Internet 80 with minimum sub-scores of 19 reading, 16 listening, 22 speaking and 18 writing), GPAs of at least 3.0 in all degrees completed, supportive letters of recommendation, and a commitment to a research career as evidenced by previous research experience and/or a strong motivation for research revealed in the personal statement section of the on-line application. All applicants we wish to pursue will be interviewed on campus unless this is not feasible (e. g. overseas international). Those students will be interviewed by phone or by Skype. The number of students admitted each year will be dependent on the quality of the applicant pool, the level of available institutional and faculty support, and the number of available laboratory openings. We anticipate recruiting an average of five to six students a year initially. Under our current arrangement with the Purdue West Lafayette Ph.D. program, students recommended for admission at IUPUI must be approved by the Purdue West

Lafayette Department of Biological Sciences. When the Biology Ph.D. is site-approved for Indianapolis, admissions will be handled on the Indianapolis campus by the faculty of the Biology Ph.D. program, using the same criteria that are used in the current Ph.D. program and overseen by the West Lafayette program. The program will serve highly qualified, research-motivated baccalaureate and masters degree holders from Indiana and beyond. Our history in offering the Ph.D. is that about 30% of our students come from IUPUI M.S. programs in Biology. The program will be primarily for full-time students although special cases may be made for students holding employment in scientific positions that can support aspects of the research, have appropriate oversight for directing research, and have no restrictions on faculty review or publication of the research findings. Student support will be derived from institutional, departmental and faculty sources. At the institutional level there are competitive first year fellowships and there are block grants to schools (distributed to departments) for one-year fellowships. In addition, there are centrally available funds to support a small number of graduate teaching assistants (TAs). All Ph.D. students must teach quarter time for at least one semester. Students whose native language in not English must pass a language test administered by the Department of English before they can be assigned to teaching. At the department level Biology has regularly supported 12-14 Ph.D. or thesis M.S. students as TAs. These students are critical to our teaching mission as they lead laboratory sections of courses in our growing undergraduate program. Finally, a number of students are supported on faculty research grants as Research Assistants (RA). Faculty are strongly encouraged to include graduate support lines in their grant proposals. Recently, the School of Science has committed more funding for graduate students and has taken over the costs of most of the tuition for graduate students. The increased resources have allowed us to raise stipends for the first time in years and to increase the number of students supported. This is critical in the support of the research faculty recently hired and those who will come on board when (2013) a new building is opened. Current support for TA or RA positions is a stipend of $22,000 for Ph.D. students and $18,000 for M.S. students. We plan to raise stipends for both degrees another $2,000 by 2012 in order to be competitive with other Ph.D. granting institutions in the state and nationally. Both TAs and RAs receive tuition remissions and paid health insurance premiums. Other program requirements include selecting a Research Advisor, passing a Qualifying Exam, establishing a Graduate Advisory committee, submitting a Plan of Study, passing a Preliminary Exam, and defending and submitting a thesis. Overall, the Purdue Indianapolis Ph.D. in Biology will be operated and managed in largely the same way as the current Purdue West Lafayette Ph.D. in Biological Sciences. Students who continue on from an IUPUI M.S. degree may select a Research Advisor at the outset of the Ph.D. Those new to IUPUI will do laboratory rotations for the first fall semester and from that experience will select a Research Advisor in December. The selection of the Research Advisor will determine the written Qualifying Exam that will be taken the following May. In 1993 IUPUI Biology was empowered to write and administer Qualifying Exams in areas that reflected the strengths of the department at that time. The department currently writes exams in Biochemistry & Molecular

Biology, Cell and Developmental Biology, Immunobiology, and Membrane Biology. Because our faculty composition has changed and will continue to do so and because research foci change over time we will likely abandon some Qualifying Exam areas and institute appropriate new ones. In December, faculty groups in the four exam areas who will write the exams prepare reading materials and reading lists for students taking those exams. In May the exams are given in two parts. The first part consists of a written exam where students are asked to write complete responses to several questions chosen from a battery of questions prepared by the faculty. The exam lasts for up to seven hours. The second part of the exam allows the student to select one question/problem from among several more open-ended options. Students have three days to prepare a typed, referenced response to the question. Faculty members who write the individual questions chosen for both parts of the exam evaluate the responses. In cases where the response for any question is on the borderline for passing a second faculty reader will evaluate. In those cases where a passing performance does not result, the student is allowed one opportunity to retake the exam. Within six months of passing the Qualifying Exam, the student establishes a Graduate Advisory Committee. At the minimum the Committee is comprised of the Research Advisor, two other faculty members from the department and one faculty member from outside of Biology. The Committee is chaired by one of the department faculty members other than the Research Advisor. Under the current Ph.D. arrangement the Committee is chaired by a faculty member from the Purdue West Lafayette Department of Biological Sciences. The proposed Ph.D. would not require a committee member from the PUWL Department of Biological Sciences. One could be invited based on an expertise match with the students research area. Once formed, the Committee meets with the student and establishes a Plan of Study. This document lists all courses and seminars the student is to complete. Research credits are not listed. Submission of this document to the Graduate School also establishes the membership of the Graduate Advisory Committee. Six months after the Plan of Study has been approved, the Preliminary Exam takes place. The Graduate Advisory Committee functions as the examining committee. This exam is an oral exam that is a defense of a written thesis proposal. The Committee will receive a comprehensive research proposal two weeks before the exam and at the exam will hear a presentation on research progress to that point and a projection as to the direction of the research, what experiments will be performed and what outcomes are expected. Passing this exam officially admits the student to candidacy for the Ph.D. The Graduate Advisory Committee will continue to meet with the student every six months to help direct the research and to monitor progress. The Purdue West Lafayette Ph.D. requires only annual meetings but national data (Ph.D. Completion and Attrition: Policies and Practices to Promote Student Success, Council of Graduate Schools, Ph.D. Completion Project, 2010) show that more frequent meetings are more effective in monitoring and assuring student progress. A written progress report, on the expected annual meeting, will be filed with the Purdue Graduate School the new mid-year meeting will be recorded and monitored internally. The Committee will also serve as the thesis examining committee at the end of program.

3. The program curriculum The Ph.D. curriculum pattern we have been following for the past twenty years has served Biology very well. Because there are no core requirements, the course work component of the Ph.D. can be individually crafted. This is particularly important in Biology, a discipline that covers a wide range of sub-disciplines from environmental biology/ecology to molecular biology and can also be partitioned along organismal lines (plant, microbe and animal). Finding an acceptable core that would serve all of these areas would not be possible. Thus, a Plan of Study would list two seminar experiences (BIOL 69600, Seminar) where the student delivers one-hour presentations on subjects outside of the research area and a set of courses, designed by the Graduate Advisory Committee, that would support and facilitate the research area. One or both of the Seminar requirements can be satisfied by giving similar presentations within graduate courses. The overall expectation of the Purdue Indianapolis Ph.D. program in Biology is one that is heavily research-oriented with the thesis and publications derived from the research being the primary outcomes. Below are Plans of Study patterned after plans for two current Purdue Ph.D. students in Biology at IUPUI and one model plan for a student coming into the program straight from the Baccalaureate. Six hours is the minimum number of hours needed to qualify for full-time status for domestic students at IUPUI. Student #1 This student had 6 credit hours of graduate course work (BIOL 50700, Prin. Molecular Biology, 3 cr. and BIOL 54800, Techniques in Biotechnology, 3 cr.) taken as excess undergraduate course work that are eligible for transfer into the thesis M.S. These six hours appear on the Ph.D. Plan of Study as well. During the M.S. the student registered for an additional six hours (BIOL 69700, TPCS; Plant Physiology, 3 cr. and BIOL 55000 Plant Molecular Biology, 3 cr.) of graduate course work. The M.S. degree is worth a maximum of 30 credits toward the Ph.D. Seminar (BIOL 69600) requirements were satisfied within two of the graduate courses. First semester: BIOL 54000 Biotechnology BIOL 69800 M.S. Research* 3 cr. hrs. 6 cr. hrs.

*Students had to register for M.S. research until the Preliminary Exam was passed. This rule has now changed and Ph.D. students can now register for BIOL 69900 Research (for Ph.D. students) Second semester: BIOL K483 Biological Chemistry BIOL 69800 M.S. Research (Qualifying Exam passed) 3 cr. hrs. 6 cr. hrs.

Third semester: Fourth semester:

BIOL 69800 M.S. Research 9 cr. hrs. (Grad. Advisory Committee formed and Plan of Study filed) BIOL 69700 TPCS: Plant Molec. Genet. BIOL 69800 M.S. Research (Preliminary Exam passed) 3 cr. hrs. 6 cr. hrs.

Fifth semester: Sixth semester: Seventh Semester Eighth semester:

BIOL 69900 Research BIOL 69700 TPCS: Plantomics BIOL 69900 Research BIOL 69700 TPCS: Plant Genetics BIOL 69900 Research BIOL 69900 Research

9 cr. hrs. 3 cr. hrs. 6 cr. hrs. 3 cr. hrs. 3 cr. hrs. 6 cr. hrs.

Total: 30 hours for M.S. plus 66 hours as Ph.D.; 27 cr. hrs. of course work. Student #2 This student has completed a non-thesis M.S. that was comprised of 30 hours of eligible course work. All are included on the Plan of Study. One seminar (BIOL 69600) was satisfied within a graduate course. First semester: BIOL 59500 Special Assignments (Laboratory rotations) BIOL 69700 TPCS: Molec. Bone Biol. (Chooses Research Advisor) BIOL 69700 TPCS: Stem Cell Biol. BIOL 69900 Research (Qualifying Exam passed) 3 cr. hrs. 3 cr. hrs. 3 cr. hrs. 6 cr. hrs.

Second semester:

Third semester:

GRAD G819 Basic Bone Biol. 3 cr. hrs. BIOL 69900 Research 6 cr. hrs. (Grad. Advisory Committee formed and Plan of Study filed) BIOL 69900 Research (Preliminary Exam passed) GRAD G651 Intro. Biostatistics I BIOL 69900 Research GRAD G825 Adv. Tpcs. Molec. Biol. BIOL 69900 Research BIOL 69900 Research BIOL 69600 Seminar BIOL 69900 Research 9 cr. hrs. 3 cr. hr. 6 cr. hrs. 3 cr. hrs. 6 cr. hrs. 9 cr. hrs. 1 cr. hr. 5 cr. hrs.

Fourth semester: Fifth semester: Sixth semester: Seventh semester: Eighth semester:

Total: 30 hours for M.S. plus 69 hours as Ph.D.; 45 cr. hrs. of course work.

Student #3 This student is entering the program straight from the Baccalaureate degree and has no transferrable credits. First semester: BIOL 59500 Special Assignments (Laboratory rotations) BIOL 50700 Prin. Molec. Biol. BIOL 56600 Developmental Biol. (Chooses Research Advisor) BIOL 69700 TPCS: Stem Cell Biol. BIOL 69900 Research (Qualifying Exam passed) 3 cr. hrs. 3 cr. hrs. 3 cr. hrs 3 cr. hrs. 6 cr. hrs.

Second semester:

Third semester:

BIOL 55900 Endocrinology 3 cr. hrs. BIOL 69900 Research 6 cr. hrs. (Grad. Advisory Committee formed and Plan of Study filed) BIOL 56800 Regen. Biol. & Medicine BIOL 56100 Immmunology BIOL 69900 Research (Preliminary Exam passed) GRAD G651 Intro. Biostatistics I BIOL 69900 Research BIOL 57100 Devel. Neurobiology BIOL 69600 Seminar BIOL 69900 Research BIOL 69900 Research BIOL 69600 Seminar BIOL 69900 Research BIOL 69900 Research BIOL 69900 Research 3 cr. hr. 3 cr. hr. 3 cr. hrs 3 cr. hr. 6 cr. hrs. 3 cr. hrs. 1 cr. hr. 5 cr. hrs. 9 cr. hrs. 1 cr. hr. 8 cr. hrs. 9 cr. hr. 9 cr. hr.

Fourth semester:

Fifth semester: Sixth semester:

Seventh semester: Eighth semester: Ninth semester: Tenth semester:

Total: 90 hours as Ph.D.; 24 cr. hrs. of course work. In the above examples all courses listed are currently offered or were offered at that time for students #1 and #2. Courses do change as faculty members come and go. However, for the past several years the Department of Biology has offered ten graduate courses per semester. New faculty who come in will add at least one new graduate course or re-introduce a graduate course currently on the books for which we had lost the instructional expertise. In addition to the ample courses in Biology our students can

also take relevant course work from departments in the IUSM (see GRAD courses for students #2 and #3 above) as well as other School of Science departments. We have the option to offer new courses under an approved variable title number (BIOL 69700) that allows us to launch courses immediately while formal approvals, under permanent numbers and titles, are sought. Appendix A lists the graduate courses in Biology and their frequencies of offering over a three year period from 2008-09 through 2010-11. 4. Form of recognition The degree offered would be a Purdue University Doctor of Philosophy (Ph.D.) in Biology through the Purdue University Graduate School. The degree would be offered exclusively on the Indianapolis campus. At the time of degree creation campus administration will identify an appropriate CIP code that will be approved by the Indiana Commission on Higher Education. The diploma will read Doctor of Philosophy, Purdue University School of Science, Indianapolis. 5. Program faculty and administrators All individuals listed hold the Ph.D. Name Gregory Anderson *Simon Atkinson *Martin Bard *Teri Belecky-Adams *Bonnie Blazer-Yost Hua-Chen Chang *Ellen Chernoff *Guoli Dai Andrew Kusmierczyk N. Douglas Lees *Jiliang Li *Anna Malkova Kathleen Marrs *James Marrs Jason Meyer *Stephen Randall *Randall Roper *David Stocum *David Skalnick Xianzhong Wang *John Watson Rank Assistant Professor Professor Professor Associate Professor Professor Assistant Professor Associate Professor Assistant Professor Assistant Professor Professor Assistant Professor Associate Professor Associate Professor Associate Professor Assistant Professor Associate Professor Assistant Professor Professor Professor Associate Professor Associate Professor Specialty Microbiology Cell Biology Genetics Developmental Biol. Physiology Immunology Developmental Biol. Regenerative Biol. Biochemistry Microbiology Bone Biology Genet./Molec. Biol. Biology Education Developmental Biol. Developmental Biol. Plant Cell Biol. Genet/Devel. Biol. Regenerative Biol. Molecular Biology Ecology Plant. Molec. Biol. Appointment Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time Full-time

*faculty members who have trained or currently are training Ph.D. students As of 9/1/10 Dr. Atkinson became the department Chair in Biology and thus will be head of the graduate program in Indianapolis. From 1982 and 1992 until 8/31/10 Dr. Lees served as Director of Graduate Programs and Chair of Biology, respectively. Effective 9/1/10 the role of Director of Graduate Programs transitioned to Dr. Bard.

Full Curriculum Vitae for all full-time faculty members participating in training Ph.D. students and administering the proposed Ph.D. program can be found in Appendix C. In addition to the full-time faculty Biology has six active adjunct (official IUPUI appointments) faculty members whose primary appointments are at the IUSM, IUSD, Methodist Research Institute, and Eli Lilly. These individuals also hold special graduate certification with the Purdue Graduate School that allows them to serve as outside members of Ph.D. and thesis M.S. committees. There are an additional ten scientists (IUSM, Eli Lilly, other IUPUI units)) who also hold special graduate certification with the Purdue Graduate School. Because we have been operating a Ph.D. program for about twenty years and because our faculty numbers have been restored in recent years (six in the last two years) there are no new positions that are required. In addition, the opening of a new building in 2013 will allow for the research space to further expand the Biology faculty. Thus, the need for additional Ph.D. students to drive faculty research and to provide instructional support for the large and growing undergraduate teaching mission will increase. 6. Needed learning resources. We anticipate no need for additional learning resources. We have at our disposal the libraries on campus that have been sufficient along with on-line resources to successfully operate all aspects of Ph.D. training in Indianapolis. Other resources on the technical side are available through core facilities in the School of Science and at the IUSM. In addition, the Indiana Clinical and Translational Sciences Institute provides access to core facilities at Purdue University, West Lafayette, Indiana University Bloomington and at the University of Notre Dame. 7. Other program strengths We are a small department relative to the size of the IUPUI campus especially in light of its the health and life sciences emphasis. Despite the fact that we cover all of the essentials of a sound undergraduate curriculum, offer an array of appropriate service courses, and deliver a highly respected fast-track, non-thesis M.S., the department has still been able to develop a strong research focus. In 1992 we identified cell & molecular biology as the level of organization on which to concentrate our hires where possible. As new molecular tools of investigation made solving complex questions more possible that focal area later expanded to encompass developmental biology. This core focus evolved over time and resulted in the formation of the Center for Regenerative Biology & Medicine (CRBM), an Indiana University Center of Excellence. There are several faculty members in the department with CRBM affiliation as well as others on campus spanning departments in science and the professional schools. This clearly gives us a

distinction on campus that is fully compatible with the research goals in the Schools of Medicine and Dentistry as well as the program in Biomedical Engineering. The Department of Biology has had a long-term commitment to including undergraduate students in the research enterprise. The presence of undergraduate students as active participants in research is a distinctive feature of the research environment in Biology and provides opportunities for graduate students to gain experience in mentoring and directing research projects. C. PROGRAM RATIONALE 1. Institutional factors. IUPUI has identified itself as the Urban Research University of Indiana. The Indiana University system has labeled IUPUI as the Health & Life Sciences campuses in the state because of the presence of a contingent of large health schools on campus. Furthermore, the IUPUI Vision Statement is very clear about the future direction of the institution. The IUPUI Vision is to be the best urban research university by conducting world-class research, scholarship, and creative activities that develop knowledge and contribute to the economic growth and social advancement of Indiana and the nation and benefit humanity as a whole. These three declarations coupled with the natural maturation of the IUPUI campus make doctoral programs not just an aspiration but also a need in central Indiana, the home of many life and health sciences industries. Building the research enterprise in the School of Science has been on the agenda for at least three decades. The Department of Biology began offering the masters degree in the late 1970s and admitted its first Ph.D. student in the late 1980s. We are not new to this endeavor but we are on the cusp of a major expansion. With the growth of the student population, the recruitment of better and better faculty, and the earning of increased external grant awards the department will seek to double its graduate student (thesis M.S. and Ph.D.) population in the next three years. In the departments most recent (2006) external program review, the review team concluded that: The number of faculty, the lab space, the number of Ph.D. students and the number of Post docs are half or in some cases, less then half, of what one might expect. While there are several lagging elements in this statement our new building will address the space shortage, the current hiring rate with more new positions planned builds the faculty, and enhanced external funding has already increased the number of post-docs by 50%. The number of Ph.D. students is the remaining piece that will be addressed with an enlarged, free-standing Purdue Ph.D. program in Biology. On a campus where many schools train a wide variety of health care professionals, a Department of Biology is a key element. At IUPUI Biology provides fundamental education for students seeking admission to nursing, dental hygiene, physical therapy, and a number of other health-related programs in the Schools of Medicine, Dentistry

and Health & Rehabilitative Sciences. In addition we train students at the Baccalaureate and Masters levels for application to medical and dental schools on campus as well as for programs in pharmacy, optometry and veterinary medicine elsewhere in the state. These responsibilities along with those associated with providing service courses to the general undergraduate population and graduating undergraduate majors who have the intention of joining the life sciences work force make our programs among the largest and fastest growing on campus. Demand for our programs at all levels is high and we must continue to work at garnering new space and recruiting excellent new faculty to help with this important work. Our recent efforts have resulted in a newly approved building with teaching and research laboratory space. Our rejuvenated school has allowed for the hiring of six new faculty members in the last two years with the promise of additional hires once the new space is finished. Our concept of the Urban Research University does not limit research to professional schools but rather raises the expectations across campus in order to elevate all units and provide multiple opportunities for cross fertilization through collaboration. Our commitment to research is made clear in all of our hires. In order for us to be able to recruit top faculty talent and in order for them to be successful when they arrive we must have a robust, high quality graduate program at the Ph.D. level. We have already seen the impact of such programs over the past twenty years on faculty productivity, external funding success, and the overall intellectual climate of the department. We are also aware of the impact that these scientists-in-training can have as teaching assistants in our undergraduate laboratory courses. They are essential in educating our undergraduate students while at the same time earning teaching experience and credentials. Our hope is to have this proposal approved for implementation as soon as possible. The program itself will seamlessly replace the one we are currently working through so it will not impact other IUPUI units or those elsewhere in the state. The resources needed would be the same except for those associated with our plans for growth. We intend to generate those resources through increased enrollments and external funding success. 2. Student demand. We project admitting five to six new Ph.D. students each year for the next two to three years. Beyond that our target number for recruitment will depend on faculty expansion that will commence when the new building opens (2013). Over the past decade we have been admitting one to three students per year. Because we have grown in the number of faculty with active research programs and in external funding that includes graduate student support lines, we anticipate that we will and must double the number of annual admits. We do not foresee a large Ph.D. program but rather modestly sized one where we can emphasize the quality of the student experience. We anticipate a steady-state enrollment of around 30. We have data on Ph.D. students thus far trained in Biology as well as the students currently enrolled in the program. The vast majority of our graduates gave gone on to successful careers in higher education and industrial or governmental positions. Below is a list of our Ph.D. graduates to date. Five of the students were IU Ph.D. students

through programs (Medical Biophysics: Ehringer, Dumaual, Shaikh, Katzer) and departments (Microbiology & Immunology: Scherer) at the IUSM in which their Biology faculty advisors held faculty appointments or had special arrangements.
1992 Christine OHara Thesis: The Role of Ependymal Calls in Axolotl Spinal Cord Regeneration. Thesis Advisor: Ellen Chernoff First Position: Post-doc, Washington University St. Louis Current Position: MBA/Bank Exec. 1993 William Ehringer Thesis: The Structure and Function of Omega-3 Polyunsaturated Fatty Acids in Model and Cellular Membranes Thesis Advisor: William Stillwell First Position: Post-doctoral, University of Louisville School of Medicine Current Position: Faculty, University of Louisville School of Medicine 1996 Beth Arthington-Skaggs Thesis: Analysis of Three Ergosterol Biosynthetic Genes Involved in the Terminal Portion of the Pathway in Saccharomyces cerevisiae Thesis Advisor: Martin Bard First Position: Post-doctoral, Center for Disease Control, Atlanta Current Position: Staff, Center for Disease Control, Atlanta 1998 Rajnish Khanna Thesis: Light-Regulated Protein Kinases from Plants Thesis Advisor: John Watson First Position: Post doctoral, Ohio State, Current Position: Scientist, Mendel Biotech Darren Seals Thesis: Elucidation of the Biochemical and Physiological Function of the Vacuolar Annexin, VCaB42. Thesis Advisor: Stephen Randall First Position: Post doctoral, Dartmouth College, Current Position: Faculty, Wake Forest University 1999 Scherer, John Thesis: Docosahexaenoic Acid Inhibits Lymphocyte Cell Cycle Progression Thesis Advisor: Laura Jenski First Position: Post-doctoral, Walter Reed Army Medical Center Current Position: N/A 2000 Alfred Dumaual Thesis: Docosahexanenoic Acid (DHA) Induced Lateral Phase Separation in Model Membrane Bilayers and Monolayers Thesis Advisor: William Stillwell First Position: Post-doctoral, University of Virginia Current Position: Management, IDEM Matthew Kennedy

Thesis: Reguiatory Analyses of Squalene Synthase (ERG9) from Saccharomyces cerevisiae and the Cloning and Characterization of The C-4 Sterol MethyJoxidase Gene (ERG25) From Candida albicans. Thesis Advisor: Martin Bard First Position: Post doctoral, University of California, Los Angeles Current Position: Faculty, Ohio State University 2001 Brian Downes Thesis: Regulated Expression of a Soybean -Expansion Gene. Thesis Advisor: Dring Crowell First Position: Post-doctoral, University of Wisconsin-Madison Current Position: Faculty, St. Louis University Denise Slayback Thesis: Genetic Influences on the Development of Chronic Graft-Versus-Host Disease Thesis Advisor: Ruth Allen First Position: Post-doctoral, University of Arizona, Current Position: Academic Specialist, IUPUI Kyle Sloop Thesis: Molecular Characterization of Mammalian Pituitary Transcription Factors. Thesis Advisor: Simon Rhodes First Position: Scientist, Lilly Current Position: Scientist, Lilly 2002 Gretchen Parker Thesis: Molecular Analysis of the LHX3 Neuroendocrine Transcription Factor Protein Thesis Advisor: Simon Rhodes First Position: Post-doctoral, Indiana University School of Medicine Current Position: Regulatory Analyst, Safis Solutions (Indiana) 2004 Saame Shaikh Thesis: The Role of Docosahexaenorc Acid in Phase Separation from Lipid Rafts: Implications for Cellular Signaling Thesis Advisor: William Stillwell First Position: Post-doctoral, Johns Hopkins University Current Position: Faculty, East Carolina University School of Medicine Sean Werner Thesis: Characterization of PRL-1 and PRL-2 as Human Oncogenes. Thesis Advisor: Pamela Crowell First Position: Post-doctoral, Lilly, Current Position: Scientist, Cook Biotech Dean Wiseman Thesis: Mechanism of Action of, and Combination Chemotherapy with Isoprenoids Perillyl Alcohol, Farnesol, and Geraniol in in vitro and in vivo Models of Human Pancreatic Adenocarcinoma Thesis Advisor: Pamela Crowell First Position: Post-doctoral, University of Montana/University of Georgia Current Position: Research Assistant Professor, IU School of Medicine 2005

Michael Katzer Thesis: Biophysical Characterization of the Interaction between a Naturally Occurring Solute, the Plant Hormone ct-Abscisic Acid, with Membranes Thesis Advisor: William Stillwell First Position: Post-doctoral, University of Buffalo Current Position: Returned to Germany Jesse Savage Thesis: In Vivo Analyses of the LHX3 and LHX4 Pituitary Transcription Factors. Thesis Advisor: Simon Rhodes First Position: Indiana University School of Medicine, class of 2009 Current Position: Resident in Neurosurgery, University of Virginia School of Medicine Aaron Santner Thesis: Light- and Auxin-Regulated Protein Kinases from Arabidopsis thaliana Thesis Advisor: John Watson First Position: Post-doctoral, Indiana University, Bloomington Current Position: Scientist, Molecular Kinetics (Indiana) 2007 Chad Hunter Thesis: Mechanisms of Gene Activation by Pituitary Transcription Factors. Thesis Advisor: Simon Rhodes First Position: Post-doctoral, Vanderbilt University Current Position: Post-doctoral, Vanderbilt University 2008 Charity Nofziger Thesis: Glitazone-Induced Changes in Arg8Vasopressin-Stimulated Ion Transport in MDCK-C7 Cells Thesis Advisor: Bonnie Blazer-Yost First Position: Post-doctoral, Paracelsas University Current Position: Post-doctoral, Paracelsas University Rachna Sehgal Thesis: Pax2 Overexpression and Regulation Studies in Vertebrate Eye. Thesis Advisor: Teri Belecky-Adams First Position: Scientist, Genentech Current Position: Scientist, Genentech 2009 David Huizinga Thesis: The Role of Isoprenylcysteine Methylation and Metabolism in Phytohormone Signaling Thesis Advisor: Dring Crowell First position: Senior Biologist, Dow AgroSciences Current Position: Senior Biologist, Dow AgroSciences 2010 Stephanie Colvin Thesis: An Animal Model of Pediatric Combined Pituitary Hormone Deficiency Disease Thesis Advisor: Simon Rhodes First position: Post-doctoral, IU School of Medicine Current Position: Post-doctoral, IU School of Medicine

We have a highly qualified group of students in the Ph.D. pipeline with eleven out of thirteen holding a Masters degree. Four of the M.S. degreed students earned that degree at IUPUI while two others (Deem and Zambrano) started as M.S. students at

IUPUI but switched to the Ph.D. before completing the M.S. Thus, our M.S. program has been an excellent and dependable source of Ph.D. students. Ph.D. students currently enrolled include the following: Student Amiraj Banga Joshua Blazek Angela Deem Kelly Koehler Sudhanshu Kumar Paul Miller Pooja Muralidharan Sreejith Ramakrishnan Deborah Sarria Mary Sherfick Yuji Yamasaki Carmen Zambrano Hongkang Zhou Academic History B.S./M.S. Punjab Agric. U. B.A. Wabash, M.S. IUPUI B.A. Taylor, M.A. Ball State B.S. Texas A&M, M.S. IUPUI B.S. Tamil Agric. U., M.S. Manipal U. B.S./M.S. U. Illinois UC B.S. U. Mumbai, M.S. Maduri Kamaraj U. B.S. Bangalore U., M.S. Kasturba Med. Col. B.A. DePauw B.S./M.S. IUPUI B.S. Tokyo U. Pharm & Life Sci., M.S. IUPUI B.S. Ball State B.S. Cent. U. National., M.S. San Francisco St. Ph.D. Advisor Blazer-Yost Roper Malkova Randall Dai Stocum Rotations Malkova Chernoff Watson Randall Randall Li

Each year the department receives fifteen to twenty applications for the Ph.D. and at least as many inquiries. This had been achieved with little to no effort at active recruiting. We are now in a position where additional students are needed for our teaching and research missions and where additional resources have been made available for graduate student funding. We plan on aggressive recruiting over the next three years such that five to six new Ph.D. students can be added each year. 3. Transferability This is not a critical issue for Ph.D. students. We have admitted a few students who began Ph.D. work elsewhere and have allowed relevant course work to transfer. 4. Access to graduate and professional programs. Again, this is not a major issue although one of our Ph.D. graduates (Jesse Savage, Ph.D. 2005) did go on to complete medical school (M.D., IUSM, 2009). 5. Demand and employment factors. Based on the success of our graduates (see Section C2) over the past two decades we are confident that future graduates will continue to find excellent positions in industry, government and academia. To date twenty-three students have earned their Ph.D. degrees in Biology at IUPUI. Eighteen have done so under the Purdue West Lafayette Ph.D. and five have been students in Indiana University Ph.D. programs. The summary of the placement outcomes for these graduates include six who are in academic positions, seven who work in industry, and three who have found laboratory or management positions in government. Five graduates remain in training positions (post-doctoral, research professor or medical resident) while three are presently in unknown positions or have gone on to other careers. Of the total seven are

permanently employed in Indiana and two others are undergoing further training in the state. 6. Regional, state and national factors. There are several Biology or Life Sciences Ph.D. programs in Indiana with the largest being at Indiana University Bloomington (IUB) and Purdue University West Lafayette (PUWL), the program through which we are now operating. The IUPUI campus also has Ph.D. programs in the Schools of Dentistry and Medicine, with the latter being significant in size. There are also Ph.D. programs at the University of Notre Dame and Indiana State University in Terra Haute. Each of these programs has a focus or flavor that makes each different in some but not in all ways. For example, biochemists may be on staff everywhere and all will direct research in some aspect of biochemistry. However, at other levels and in certain life sciences sub-disciplines, clear distinctions emerge. PUWL has revamped its research into three areas. 1. Development and Disease. This encompasses many sub areas including developmental biology, neuroscience, plant biology, cancer biology, and microbial pathogenesis. 2. Ecology and Evolution. 3. Molecular Biosciences IUB claims a research program of breadth and depth and identifies 11 research clusters that have recently been reduced to the following 3 programs: 1. Evolution, Ecology and Behavioral Science 2. Microbiology 3. Molecular, Cellular and Developmental Biology Indiana State lists 3 areas of research focus. 1. Cell & Molecular Biology 2. Ecology, Systematics and Evolution 3. Physiology The University of Notre Dame advertises a wide range of research opportunities but does combine them into four fields of study. 1. Cellular and Molecular Biology 2. Developmental Biology 3. Ecology, Evolution and Environmental Sciences 4. Immunology, Microbiology and Global Health The IU Schools of Medicine and Dentistry have research areas in the standard basic sciences with the obvious emphases on the appropriate applied areas of human health.

Biology at IUPUI is relatively small in terms of faculty count (although growing) and has Ph.D. opportunities in several areas including immunology, molecular biology, microbiology, plant biology, biochemistry and development. As already mentioned its major focal area is in regenerative biology. This focus includes developmental biologists and is synergistic with and complementary to research being done in medicine, dentistry and biomedical engineering. Within the Biology faculty in the CRBM, there are labs working on limb, liver, and spinal cord regeneration, induced pluri-potent stem cells (iPS), and retinal development and repair. The CRBM has been magnet for graduate interest, as it is one of just a few such centers in the U.S. Beyond the uniqueness of the CRBM, there are Indianapolis area residents with strong interests in graduate education who cannot relocate for spousal and other personal reasons. Unless they a can be served by campus Ph.D. programs in the professional schools, we are their only option. Eight of our Ph.D. graduates and four of our current students had/have such personal issues. D. PROGRAM IMPLEMENTATION AND EVALUATION There are no special steps we must take to implement the program. It will be operated in the same way as the current Purdue West Lafayette program except for the need to pass applications through the Department of Biological Sciences for approval and the necessity of PUWL faculty representation on students Graduate Advisory Committees. Students in the current program will migrate to the new program provided they are not too far along in their studies and provided that there are no burdensome procedures required. The program will be evaluated using the following parameters: 1. Number of applicants and admitted students 2. Number of students attending 3. Number of students supported on grants and from institutional sources 4. Profiles of attendees (GPAs, GRE scores, graduate degrees, previous institutions attended) 5. Student performance in course work 6. Student performance on Qualifying, Preliminary and Final Exams 7. Student research productivity (number of publications, quality of media, presentations) 8. Awards and other special recognition 9. Time to degree 10. Number of graduates 11. Student placement: Number placed and quality of placement Monitoring the above parameters will be the responsibilities of the Director of Graduate Programs and the Biology Graduate Committee. Each year in early fall the recruiting data (items 1 and 2) from the previous year will be collected and summarized. Historical date (item 4) on matriculating students will also be compiled at that time. The individual annual cohorts will be followed through graduation to collect the information for item 10 as well has item 9. Pass rates on the Qualifying and Preliminary Exams will be compiled each spring and performance on the Final Exam will be

collected for the previous year in the summer. Course work performance will be monitored semester by semester. Data regarding student productivity, awards and placement will be followed for three to five years post graduation to capture program productivity that appears after leaving and to allow for a long-term view of career trajectory. We will also monitor annually the proportion of institutional and grant supported students. It is an important measure of program effectiveness that institutional commitment and external research funding success be in balance. We are prepared to change whatever is necessary if student performance does not measure up to expectations. However, a single case usually does not call for intervention. What follows are steps that may be taken in cases where there are multiple examples of poor performance. In cases where the data indicate poor to mediocre performance on Qualifying Exams we will review recruitment data, preparatory materials made available to students, and courses taken prior to the exam. Solutions could involve changes in preparatory materials, changes within courses or among courses selected, and requiring more background of students admitted to certain areas of study. In cases of poor classroom performance we will consider instructional effectiveness and examination materials. In some cases a change of instructor may be the best remedy. We will annually report the publications and other achievements of our students in order to publicly display expectations to all faculty mentors and students. This is a subtle way to support the notion that productivity (item 7) is a critical outcome of the training. To this point in our history of Ph.D. training there have been no recurring problems with exams or courses that have required curricular or instructor changes.

E. TABULAR INFORMATION

APPENDIX A Graduate Courses* in IUPUI Biology, 2008-09 through 2010-11 Course # Title # over 3 years 50700 Principles Molecular Biology 3 51600 **Molecular Biology Cancer 1 54000 Biotechnology 2 55000 Plant Molecular Biology 3 55600 Physiology I 3 55700 Physiology II 3 55900 Endocrinology 3 56100 Immunology 3 56400 Molecular Genetics Development 3 56600 Developmental Biology 3 56800 Regenerative Biology & Medicine 6 57100 Development Neurobiology 3 69700 TPCS: Sensory Systems 3 69700 TPCS: DNA Mutagen., Recomb., Genomics 3 69700 TPCS: Cell & Molec. Bone Biology 3 69700 TPCS: Immune System Disorders 3 69700 TPCS: Stem Cell Biology 3 69700 TPCS: Microbial Genetics 3 69700 TPCS: Cell Biology 1 69700 TPCS: Plantomics 1 69700 TPCS: Plant Genetics 1 69700 TPCS: Plant Molecular Genetics 1 * all courses are 3 credits ** currently not offered due to the loss of a faculty member Graduate Course Descriptions We are in the process of updating course descriptions for 500 level courses to more accurately reflect the current state of knowledge in each sub-discipline. 50700 Principles of Molecular Biology. P: K322, CHEM C342, or consent of instructor. Molecular aspects of structure and function of nucleic acids and proteins, including recombinant DNA research. Prokaryotic and eukaryotic molecular biology are given equal weight. 51600 Molecular Biology of Cancer. P: CHEM C342 and K322 or a course in biochemistry. A detailed course examining the molecular mechanisms controlling the growth of animal cells. Emphasis on current experimental approaches to defining the molecular basis of growth regulation in developing systems and the uncontrolled proliferation of cells in metabolic disorders, such as cancer. Semester F S F or S F F S F&S S S F F&S S F F F S or F S or F S S S S S

54000 Topics in Biotechnology. P: K322 and CHEM C341, or consent of instructor. Examines research techniques and applications for several technologies situated at currently recognized biological frontiers, including recombinant DNA technology, hybridoma technology, protein engineering, agricultural research, and microbiological engineering. 55000 Plant Molecular Biology. P; K322, CHEM C341, or consent of instructor. A comprehensive study of plant molecular biology and plant molecular genetics. Topics will include structure and expression of plant nuclear, chloroplast and mitochondrial genomes, and plant viruses. 55600 Physiology I. P: K103 and CHEM C342. Principles of physiology: nerve and muscle, temperature regulation, ion and water balance. 55700 Physiology II. P: 55600 or consent of instructor. A study of human cardiovascular, pulmonary, blood, and gastrointestinal systems. Higher neuronal functions and intersystem interactions will be discussed. 55900 Endocrinology. P: 55600 or equivalent, and CHEM C342. The study of hormone function. Consideration will be given to the role of hormones in growth, development, metabolism, homeostasis, and reproduction. 56100 Immunology. P: K103, CHEM C341. Introduction to basic principles and experimentation in cellular and humoral immunology. 56400 Molecular Genetics of Development. P: K322 or similar or consent of instructor. R: 56600. Examines how key regulatory genes and molecular signaling pathways regulate development in both lower eukaryotic organisms and mammalian organ systems, with emphasis on the function and evolution of signaling molecules and transcription factor super-families. 56600 Developmental Biology. P: K322. Principles of animal development. The emphasis is on concepts and underlying mechanisms of developing and regenerating systems and stem cell properties, including molecular and biochemical approaches. 56800 Regenerative Biology & Medicine. P: K324 or K331 or a biochemistry course. The course examines the mechanisms of natural regeneration (regenerative biology) and the application of these mechanisms to the development of therapies to restore tissues damaged by injury or disease (regenerative medicine). 57100 Developmental Neurobiology. P: consent of instructor. The major phases of nervous system development beginning with neurolation and neurogenesis and ending with the onset of physiological activity will be studied in a variety of animals, mainly avians and mammals (including man). Neural developmental disorders and behavioral ontogeny will also be considered. 69700 Special Topics*. Critical examination of developments by members of the department. This is a variable title course. Courses that have been offered (2008-09 through 2010-11) or are planned for offering in the near future are the following:

TPCS: Sensory Systems. The goal of this course is to gain an understanding of the mechanisms that underlie sensory perception at the molecular, cellular, and systems level. This is accomplished by examining how various forms of energy are transduced into the electrochemical messages of the nervous system, what pathways the information travels within the nervous system, and how this information is processed and perceived. TPCS: DNA Mutagenesis, Recombination, Genomic Rearrangements. The primary objective is to provide students with up-to-date knowledge of cellular mechanisms responsible for the maintenance of genetic stability. Besides traditional lectures, this course includes many student presentations and collective discussions. Two important goals of this course are to teach students to effectively use peerreviewed articles as an important source of information as well as to make effective and clear presentations. TPCS: Microbial Genetics. This course emphasizes various regulatory genetic mechanisms in viral, bacterial, and fungal systems. Genetic and molecular aspects of developmental and regulatory processes such as operons in bacteria, mating and cell cycle genes in yeast, and early and late gene expression in viruses are considered. TPCS: Cell & Molecular Bone Biology. This course concentrates on the cellular and molecular concepts of bone and cartilage, and applications to bioengineering concepts. The topics include bone and cartilage development and growth, signal transduction in bone cells, bone metabolic diseases, stem cells and skeletal regeneration, and bone cancer metastasis. TPCS: Stem Cell Biology. This course examines stem cells' defining features, activities and potential utility, focusing on important characteristics of stem cells and will develop a general overview of stem cell biology. The course will build on this overview of stem cell biology by examining specific examples of developmental biology, methodology and the potential applications of stem cell therapy. TPCS: Immune System Disorders. The goals of this class are to understand the fundamental mechanisms that contribute to immune system dysfunction. Genetic defects in the immune system, immune complex diseases, immune mediated hypersensitivity reactions and autoimmune diseases will be discussed. TPCS: Cell Biology. This course covers topics in which significant recent advances or controversies have been reported. It will involve an in-depth coverage of topics in cellular and sub-cellular organization, structure, and function in plants and animals. Each topic presented will consist of an introductory lecture, student presentations, and intense discussion of the experimental methods used, the results obtained, the interpretation of these results in the context of cell structure and function, and the implications for further studies. Example topics are: cell cycle, intracellular transport, vesicular trafficking, cell-cell communication, and epigenetic regulation of cellular function.

TPCS: Plant Topics. This course cycles yearly between such topics as Plant Biochemistry, Plant Genetics, Plant Physiology, Plant Molecular Genetics, Plant Omics, and Plant Development. The content is provided through faculty lectures and in student delivered presentations. TPCS: Microbial Pathogenesis (future offering). This course investigates the mechanisms used by microbes to cause disease. Using primary literature, we will discuss the role of specific virulence factors in pathogenesis as well as the interactions between microbes and the host. We will also highlight new and reemerging infectious diseases. * The first two 69700 courses listed will be submitted for permanent course status with their own 500 level numbers in 2010-11. APPENDIX B Demand and Employment Analysis Rather than speculate or project demand for this program we will rely on our experience with Ph.D. training in Biology at IUPUI. It is not typical to expect that a Ph.D. program will have significant local impact on the region in terms of permanent employment. Rather the Ph.D. is a global degree with recipients most frequently seeking high-level positions wherever they exist sometimes including opportunities in other countries. Despite this we note that seven of twenty-three of our Ph.D. graduates have found permanent (non-post-doctoral and other temporary) employment in the state. The seven include one governmental placement, one in higher education, and five life sciences placements in companies both large and small. These are listed in sections C2 and C5 along with out-of-state faculty, governmental and industrial positions taken by the rest of our graduates. Several of our graduates currently remain in post-doctoral positions and are not yet ready to seek permanent positions. We are confident that such success in placement of our graduates, both locally and beyond, will continue even as our program grows. APPENDIX C Biology Faculty Curriculum Vitae (attached)

Gregory George Anderson, Ph.D.

Curriculum Vitae
Office: Department of Biology Indiana University Purdue University Indianapolis SL 320 Indianapolis, IN 46202 Telephone: (317) 278-3896 E-mail: ga2@iupui.edu

EDUCATION
2004 Washington University in St. Louis, St. Louis, Missouri Ph.D. in Molecular Microbiology and Microbial Pathogenesis Thesis Title: Survival and Persistence of Uropathogenic Escherichia coli in the Urinary Tract, Thesis Advisor: Dr. Scott J. Hultgren 1998 Brigham Young University, Provo, Utah B.S. in Microbiology Minor in Chemistry

RESEARCH AND PROFESSIONAL EXPERIENCE

2009-present Assistant Professor, Department of Biology, Indiana University Purdue University Indianapolis, Indianapolis, Indiana 20042009 Postdoctoral Research, Department of Microbiology and Immunology, Dartmouth Medical School, Hanover, New Hampshire Doctoral Researcher, Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri Biotechnician, Anthony G. Gristina Institute for Biomedical Research, Herndon, Virginia

19992004

19981999

EDITORIAL RESPONSIBILITIES
Ad hoc reviewer for the Journal of Bacteriology, Antimicrobial Agents and Chemotherapy, Applied and Environmental Microbiology, Infection and Immunity, Journal of Clinical Microbiology, BMC Microbiology, Biomembranes, Journal of Dental Research

HONORS AND AWARDS

Research Support Fund Grant 2010, Investigating the Regulation of the Magnesium Transporter MgtE in Pseudomonas aeruginosa Biofilms Young Investigator Award, Center for Biofilm Engineering 2010 Appointed to Institutional Ruth L. Kirschstein National Research Service Award Training Grant 20082009 Awarded Postdoctoral Research Fellowship from the Cystic Fibrosis Foundation 20062008 Appointed to Institutional Ruth L. Kirschstein National Research Service Award Training Grant 20052006 Recipient of ASM Student Travel Grant Trustees Scholar, Brigham Young University 2003 19921998

PROFESSIONAL MEMBERSHIP
American Society for Microbiology, Student Member 1999, 2002present

TEACHING AND SERVICE

2010-present BIOL K356: Microbiology 2009-present Curriculum Committee member, Department of Biology, IUPUI 2009-present Biochemistry and Molecular Biology Qualifying Exam Committee, IUPUI

ORAL PRESENTATIONS
Bladder As Focus for Relapse. 2003. 13th European Congress of Clinical Microbiology and Infectious Diseases, Glasgow, United Kingdom. Intracellular Biofilms Constructed by Uropathogenic E. coli. November 6, 2003. ASM Conference on Biofilms 2003, Victoria, Canada. Biofilm Antibiotic Resistance in Pseudomonas aeruginosa. 2006. 2006 AAPS Annual Meeting and Exposition, San Antonio, Texas. Virulence of Pseudomonas aeruginosa Biofilms on Cystic Fibrosis-Derived Airway Cells. 2007. 4th ASM Conference on Biofilms, Quebec City, Canada.

The Pseudomonas aeruginosa Magnesium Transporter MgtE Affects Regulation of Type III Secretion. 2009. 2009 FASEB Summer Research Conferences, Microbial Pathogenesis: Mechanisms of Infectious Disease, Snowmass Village, CO. Eradication of Pseudomonas aeruginosa Biofilms in vitro by the Antibiotic Combination Fosfomycin/Tobramycin for Inhalation (FTI). 2009. The 23rd Annual North American Cystic Fibrosis Conference, Minneapolis, MN. Inhibition of Pseudomonas aeruginosa Toxicity Toward Airway Epithelial Cells. 2009. The 23rd Annual North American Cystic Fibrosis Conference, Minneapolis, MN. Analysis of Pseudomonas aeruginosa Biofilms on Airway Epithelial Cells. 2009. Indiana University School of Medicine, Department of Cellular and Integrative Physiology, Indianapolis, IN. Analysis of Pseudomonas aeruginosa Biofilms on Airway Epithelial Cells. 2009. Purdue University, Department of Biological Sciences, West Lafayette, IN. Regulation of Toxicity in Pseudomonas aeruginosa Biofilms by the Magnesium Transporter MgtE. 2010. Montana Biofilm Science and Technology Meeting, Center for Biofilm Engineering, Bozeman, MT.

ABSTRACTS PRESENTED
Anderson, G. G., Palermo, J. J., Roth, R., Heuser, J., and Hultgren, S. J. 2003. Bacterial Commune: The Intracellular Life of Uropathogenic E. coli. International Bladder Symposium, Arlington, Virginia. Anderson, G. G., Aspedon, A., Palmer, K., Whiteley M., and OToole G. A. 2006. Reduction of Pseudomonas aeruginosa Biofilm Formation Under Osmotic Stress. The 20th Annual North American Cystic Fibrosis Conference, Denver, Colorado. Anderson, G. G., and OToole, G. A. 2007. Virulence of Pseudomonas aeruginosa Biofilms on Cystic Fibrosis-Derived Airway Cells. The 21st Annual North American Cystic Fibrosis Conference, Anaheim, California. Anderson, G. G., Mateo, K. F., Yahr, T. L., and OToole, G. A. 2008. The Pseudomonas aeruginosa Magnesium Transporter MgtE Affects Type III Secretion. The 22nd Annual North American Cystic Fibrosis Conference, Orlando, Fl. Anderson, G. G., Kenney, T. F., OToole, G. A., MacLeod, D. L., and Henig, N. R. Eradication of Pseudomonas aeruginosa Biofilms in vitro by the Antibiotic Combination Fosfomycin/Tobramycin for Inhalation (FTI). 2009. The 23rd Annual North American Cystic Fibrosis Conference, Minneapolis, MN.

Anderson, G. G., Yahr, T. L., Lovewell, R. R., and OToole, G. A. 2009. Inhibition of Pseudomonas aeruginosa Toxicity Toward Airway Epithelial Cells. The 23rd Annual North American Cystic Fibrosis Conference, Minneapolis, MN.

PUBLICATIONS
Anderson, G. G., Palermo, J. J., Roth, R., Heuser, J., Hultgren, S. J. 2003. Intracellular Bacterial Biofilm-like Pods in Urinary Tract Infections. Science 301:105-107. Justice, S. S., Hung, C., Theriot, J. A., Fletcher, D. A., Anderson, G. G., Footer, M. J., and Hultgren, S. J. 2004. Differentiation and Developmental Pathways of Uropathogenic Escherichia coli in Urinary Tract Pathogenesis. Proceedings of the National Academy of Sciences 101:1333-1338. Anderson, G. G., Dodson, K. W., Hooton, T. M., and Hultgren, S. J. 2004. Intracellular Bacterial Communities of Uropathogenic Escherichia coli in Urinary Tract Pathogenesis. Trends in Microbiology 12:424-430. Anderson, G. G., Martin, S. M., and Hultgren, S. J. 2004. Host Subversion by Formation of Intracellular Bacterial Communities in the Urinary Tract. Microbes and Infection 6:1094-1101. Anderson, G. G., Lee, Y. M., Smith, C., and Hultgren, S. J. 2006. Mechanisms of Bacterial Adhesion and Consequences of Attachment, p. 207-246. In Nickerson, C. and Schurr, M. (Eds.), Molecular Paradigms of Infectious Disease: A Bacterial Perspective. Springer-Verlag, New York. Anderson, G. G., and OToole, G. A. 2008. Innate and Induced Resistance Mechanisms of Bacterial Biofilms, p. 85-105. In Romeo, T. (Ed.), Bacterial Biofilms. Current Topics in Microbiology and Immunology, vol. 322. Springer-Verlag, Berlin. Anderson, G. G., Moreau-Marquis, S., Stanton, B. A., and OToole, G. A. In vitro Analysis of Tobramycin-Treated Pseudomonas aeruginosa Biofilms on Cystic Fibrosis-Derived Airway Epithelial Cells. Infection and Immunity 76(4):14231433. (Selected as a Spotlight Article by the Editors) Moreau-Marquis, S., Bomberger, J.M., Anderson, G. G., Swiatecka-Urban, A., Ye, S., OToole, G. A., and Stanton, B. A. 2008. The F508-CFTR Mutation Results in Increased Biofilm Formation by Pseudomonas aeruginosa by Increasing Iron Availability. American Journal of Physiology-Lung Cellular and Molecular Physiology 295(1):L25-37. (Selected as Editorial Focus) Anderson, G. G., Yahr, T. L., Lovewell, R. R., and OToole, G. A. 2010. The Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Transcription of the Type III Secretion System. Infection and Immunity 78(3):1239-1249.

Anderson, G. G., Goller, C. C., Justice, S., Hultgren, S. J., and Seed, P. C. 2010. Polysaccharide Capsule and Sialic Acid-Mediated Regulation Promote Biofilmlike Intracellular Bacterial Communities During Cystitis. Infection and Immunity 78(3):963-975. Moreau-Marquis, S., Redelman, C. V., Stanton, B. A., and Anderson, G. G. 2010. Coculture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells. Journal of Visualized Experiments (44):e2186.

CURRICULUM VITAE SIMON J. ATKINSON, PH.D.

NAME: Atkinson, Simon J. EDUCATION: Undergraduate: Kings College, University of London, London, England; B.Sc., Biophysics, 1986 Graduate: Postdoctoral: University of Cambridge, Cambridge, England; Ph.D., Molecular Biology, 1990 The Johns Hopkins University School of Medicine, Baltimore, MD; Cell Biology, 1990-94

ACADEMIC APPOINTMENTS: Assistant Professor, Division of Nephrology, Department of Medicine, Indiana University School of Medicine; and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine. 1994-2000. Associate Professor Division of Nephrology, Department of Medicine, Indiana University School of Medicine; and Department of Biochemistry and Molecular Biology, Indiana University School of Medicine. 2000-2010. Graduate Advisor and Director Ph.D. Program in Medical Biophysics: Biomolecular Imaging. 2004-2010. Professor and Chair Department of Biology, Purdue School of Science, Indiana University Purdue University Indianapolis (IUPUI). 2010-present. Professor Departments of Medicine and of Biochemistry and Molecular Biology, Indiana University School of Medicine. 2010-present. PROFESSIONAL SOCIETIES: American Society for Cell Biology, Member British Society for Cell Biology, Member American Society of Nephrology, Member Sigma Xi, Member HONORS AND AWARDS Indiana University Trustees Teaching Award 2004

Simon J. Atkinson, Ph.D.

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BUSINESS ACTIVITES Founder, Member and Manager, INphoton LLC, Imaging services for drug development. TEACHING ACTIVITIES: a) Teaching in Medical School and Graduate School Courses 1994-2000 B/G817 Eukaryotic Cell Biology, B500 Biochemistry, F705 Molecular and Cellular Physiology, B/G807 Protein Structure, B503 Biochemistry PBL/X604 Concepts of Health and Disease, G801 Experimental Approaches to Cell Structure and Function, G760 Epithelial Cell Biology. 2000 Fall Semester B/G817 Eukaryotic Cell Biology. 10 lectures (one hour each). 30 students. F705 Molecular and Cellular Physiology. 2 seminars/discussions (one hour) for 35 students. B500 Biochemistry. 3 lectures (one hour each) for 50 students. X604 Concepts of Health and Disease. Facilitator for group of 8 medical students. 12 sessions (2 hours each). 2001 Spring Semester G807 Protein Structure and Function. 15 students. 1 one-hour lecture. 2001 Fall Semester B/G817 Eukaryotic Cell Biology. 10 lectures (one hour each). 30 students. B500 Biochemistry. 5 lectures (one hour each) for 50 students. X604 Concepts of Health and Disease. Facilitator for group of 8 medical students. 12 sessions (2 hours each). 2002 Fall Semester B/G817 Eukaryotic Cell Biology 10 lectures (one hour each). 28 Students B500 Introductory Biochemistry 7 lectures (one hour each). 50 Students. X604 Concepts of Health and Disease. Facilitator for group of 8 medical students. 12 sessions (2 hours each).

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2003 Fall Semester B/G817 Eukaryotic Cell Biology 10 lectures (one hour each). 30 Students B500 Introductory Biochemistry 7 lectures (one hour each). 65 Students. X604 Concepts of Health and Disease. Facilitator for group of 7 medical students. 12 sessions (2 hours each). G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for students in the Biotechnology Training Program. Optical Microscopy in Renal Research (Course/Workshop for 20 participants from throughout the US, offered as part of the NIH OBrien Center). Lecture and demonstrations. 2004 Fall Semester B/G817 Eukaryotic Cell Biology 10 lectures (one hour each). 30 students B500 Introductory Biochemistry 7 lectures (one hour each). 65 students. G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. F592 Introduction to Biomolecular Imaging 1 lecture (one hour) 10 students. 2005 Spring Semester F711 Cellular and Integrative Physiology 3 lectures (one hour) and one 2-hour lab/demonstration. 4 students. G613 Advanced Cellular Imaging. Co-director. 2 three-hour lecture/demonstration sessions. 6 students. 2005 Fall Semester B/G817 Eukaryotic Cell Biology 10 lectures (one hour each). 35 students G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students. F711 Cellular and Integrative Physiology One 2-hour lab/demonstration. 4 students. 2006 Fall Semester B/G817 Eukaryotic Cell Biology 10 lectures (one hour each). 35 students

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G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students. F711 Cellular and Integrative Physiology One 2-hour lab/demonstration. 4 students. F592 Introduction to Biomolecular Imaging Course director, one lecture. 7 students. 2007 Fall Semester G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students. G717 Cellular Basis of Systems Biology 2 lectures (one hour each). 45 students. 2008 Spring Semester G733 Introduction to Biological Microscopy. Course Director. 3 lectures (one hour each). 7 students. G817 Molecular Basis of Cell Structure and Function. 7 lectures (one hour each). 7 students. 2008 Fall Semester G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students. G717 Cellular Basis of Systems Biology 2 lectures (one hour each). 45 students. 2009 Spring Semester G733 Introduction to Biological Microscopy. Course Director for this 1 credit course. 3 lectures (one hour each). 8 students. G817 Molecular Basis of Cell Structure and Function. 7 lectures (one hour each). 11 students. 2009 Fall Semester G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students.

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G717 Cellular Basis of Systems Biology 2 lectures (one hour each). 45 students. 2010 Spring Semester G733 Introduction to Biological Microscopy. Course Director for this 1 credit course. 3 lectures (one hour each). 8 students. G817 Molecular Basis of Cell Structure and Function. 7 lectures (one hour each). 11 students. 2010 Fall Semester G823 Methods in Cell Biology 2 lectures (one hour each) and 4 laboratory exercises (2 hours each) for 10 students in the Biotechnology Training Program. X/G804 Cell and Molecular Biology 4 lectures (one hour each). 120 medical students. G717 Cellular Basis of Systems Biology 2 lectures (one hour each). 45 students.

Graduate Student Mentoring and Committees (since 2000) Mentor and Advisory/Dissertation Committee Chair. Hao Zhang. Ph.D. Candidate, Department of Biochemistry and Molecular Biology. July 2003-5/2009. Completed. Advisory Committee/Dissertation Committee Chair. Committee for Justin Johnson, M.D./Ph.D. Candidate, Department of Biochemistry and Molecular Biology. August 2009present. In progress. Mentor and Advisory/Dissertation Committee Chair. Committee for Peter Corridon. Ph.D. Candidate, Biomolecular Imaging and Biophysics Program. July 2010-present. In progress. Dissertation Committee: Committee for Hui Zong, Ph.D. Candidate, Department of Biochemistry and Molecular Biology. May 1999-June 2001. Completed. Dissertation Committee Committee for Paula Ladd, Ph.D. Candidate Department of Biochemistry and Molecular Biology. June 2000-Apr 2004. Completed. Dissertation Committee. Committee for Sirisha Asuri, Ph.D. Candidate Department of Biochemistry. June 2002-January 2007. Completed. Advisory Committee. Committee for Nivanka Paranavitana. M.S.. Candidate, Department of Biochemistry and Molecular Biology. 2003-2006. Completed. Dissertation Committee. Committee for Wenwu Zhang, Ph.D. Candidate, Department of Cellular and Integrative Physiology. 2003-Dec 2004. Completed. Dissertation Committee. Committee for Rong Zhao, Ph.D. Candidate, Department of

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Cellular and Integrative Physiology. January 2005-2008. Completed. Dissertation Committee. Committee for Rui Duan, Ph.D. Candidate, Department of Cellular and Integrative Physiology. May 2005-2008. Completed. Dissertation Committee. Committee for Li Fan, Ph.D. Candidate, Department of Biochemistry and Molecular Biology, June 2004-present. In progress. Dissertation Committee. Committee for Wei Luo, Ph.D./M.S. Candidate, Department of Biochemistry and Molecular Biology. July 2005- February 2008. Completed. Dissertation Committee. Committee for Jingliang Yan, Ph.D. Candidate, Department of Biochemistry and Molecular Biology. May 2006-July 2009. Completed. Dissertation Committee. Committee for Justin Babcock, Ph.D. Candidate, Department of Biochemistry and Molecular Biology. June 2007-present. In progress. Dissertation Committee Chair. Committee for Kim Jackson, Ph.D. Candidate, Graduate Program in Biomolecular Imaging and Biophysics. In progress. Dissertation Committee. Committee for Emily Horvath, M.D./Ph.D. Candidate, Department of Cellular and Integrative Physiology. April 2006-present. Completed. Dissertation Committee. Committee for Josh Salter, M.D./Ph.D. Candidate, Department of Microbiology and Immunology. March 2007-present. In progress. Dissertation Committee. Committee for Michael Kalwat, Ph.D. Candidate, Department of Biochemistry and Molecular Biology. June 2009-present. In progress. Dissertation Committee. Committee for Kris Kamocki, Ph.D. Candidate, Department of Biochemistry and Molecular Biology. June 2009-present. In progress. Administrative/Service Activities Related to Graduate Education Graduate Advisor and Director: Ph.D. Program in Medical Biophysics: Biomolecular Imaging. 2004-present. Chair, Admissions Committee. Indiana Biomedical Gateway Program. 2006-present. Other Trainees (since 2000) Preceptor for Postdoctoral Fellow: Mark Hallett, Ph.D. January 2000-December 2002. (Assistant Scientist, Assistant Research Professor IUSOM). Preceptor for Postdoctoral Fellow: Narayan Raman, Ph.D. August 1996-2001. (Research Associate, Cleveland Clinic) Rotation Students: Chad Walls, Ross Cocklin (Biochemistry and Molecular Biology Ph.D. Program), Emily Horvath, Sarah Poirier (Indiana Medical Scientist MD/PhD Combined

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Degree Program), Chris Batuello (Indiana Biomedical Gateway Ph.D. Program), Karl Koehler, Jeremiah Hendricks, (IBMG Ph.D. Students). Summer Undergraduate Student Interns: Jeremy Silk (IUSOM), Matt Muterspaugh (MIT), Ryan Kamp (IUSOM), Harsh Singh, Amanda Gosch, Matt Loesch (Loyola University Chicago), RJ Metz (IUSOM), Hilton Clarke (University of Michigan MPH, Ph.D.). PROFESSIONAL SERVICE ACTIVITIES: (1a) Treasurer, Sigma Xi Indianapolis Chapter, 1997-present. (1b) Scientific Reviewer, Journal of Cell Biology, Journal of Biological Chemistry, Cell, Biochemistry, Chromosoma, Kidney International, American Journal of Physiology, Journal of Molecular Biology, FEBS Letters, Alcohol: Clinical and Experimental Research. (1b) Chair, Special Emphasis Panel ZRG-1 SSS-G(06). Center for Scientific Review, National Institutes of Health. April 2000. (1b) Ad-Hoc Member, General Medicine B Study Section, Center for Scientific Review, National Institutes of Health. June 2001. (1b) Ad-Hoc Member, Special Emphasis Panel NIH CSR July 2002 (1b) Site Visit Committee, NIEHS Environmental Health Sciences Center, University of Wisconsin, Madison, May 6-8 2002. (1b) Special Emphasis Panel, NIDDK Review of OBrien Kidney Disease Center Applications, Dec 15-18, 2002. (1b) Special Emphasis Panel, NIDCR Review of Oral Infectious Disease Centers. May 2004. (1b) Ad-Hoc Member Physiological Chemistry Study Section NIH-CSR. June 2003, Feb 2004 (1b) Ad-Hoc Member Pathobiology of Kidney Disease Study Section NIH-CSR, July 2004 (1b) Ad-Hoc Member Special Emphasis Panels NIDDK 4/2003, 7/2004, 3/2007, 4/2010 (1b) Ad-Hoc Member Special Emphasis Panels NIH-CSR 7/2005, 10/2008, (1b) Ad-Hoc Reviewer National Science Foundation, Welcome Trust (UK), National Health and Medical Research Council (Government of Australia), BBSRC (UK) (1b) Member, Public Information Committee, American Society for Cell Biology, December 2001-present. (Chair-elect, 2010-12) (3) Experimental Demonstrations for Guion Creek Elementary Enrichment Program 3rd Grade Students, College Wood Elementary 4/5th Grade Challenge Class, Project SEED.

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UNIVERSITY SERVICE ACTIVITIES: (1) Scientific Board Indiana Center for Biological Microscopy 2001-present (2a) Graduate Admissions Committee, Department of Biochemistry, 1997-98, 2002-2005. (2a) Steering Committee Biomolecular Imaging Program, Chair 2003-present (2b) Biomedical Research Committee, School of Medicine, Member July 2001-present (2b) Awards Committee, School of Medicine, Member 2009-10 (2b) Research Cores Oversight Committee/TTR Oversight Committee, School of Medicine, Member 2004-present, Chair 2006-present. (2b) CBR/CTR Collaborative/Translational Research Grants Program Review Committee Chair 2008-present. (2b) Secretary, Secretary-Elect of the Faculty, School of Medicine. 2003-5 (2b) Search Committee for Chair, Department of Cellular and Integrative Physiology 2003. (2b) President-Elect, President, Past President of the Faculty. School of Medicine. 2005-8 (2c) IUPUI Faculty Council, School of Medicine Unit Representative. 1997-99, 20042008. (2c) President of the Faculty, IUPUI. 2008-2010. (2c) IUPUI Faculty Council Academic Affairs Committee. Member 1998-2004 (2c) IUPUI Faculty Council Budgetary Affairs Committee. Member 2003-present (2c) IUPUI Faculty Council Research Affairs Committee. Member 2003-present, Chair 2006-2008. (2c) IUPUI Faculty Council Executive Committee. Member 2007-present. (2c) IUPUI Search Committee for Executive Vice Chancellor and Dean of the Faculties 2005-6. Member. (2c) IUPUI Search Committee for Vice Chancellor for Research 2007. Member.

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(2d) Indiana University Faculty Council, Member 2000-04, 2006-present. (2d) Co-Secretary, Indiana University Faculty Council. 2008-2010. (2d) Planning Committee for Human Biology Curriculum, IU Bloomington. 2003-2004. (2d) Indiana University Intellectual Property Policy Review Committee, Co-Chair 20042005. (2d) Indiana University Intellectual Property Policy Council, Chair 2008-present. (2d) Indiana University Educational Policies Committee, Member 2000-02 (2d) Indiana University UFC University Planning Committee, Member 2001-2003 (2d) Indiana University UFC University Research Committee, Member 2003-2010 (2d) Indiana University Honorary Degrees Committee, Member 2002-2004, 2006-present, Chair 2007-2009. (2d) Indiana University Presidential Search Faculty Advisory Committee 2006-2007. Member. PROFESSIONAL ACTIVIES: Invited Lecture: American Society of Nephrology Annual Meeting, San Antonio, TX. November, 1998. Visiting Professor Division of Nephrology and Hypertension, Henry Ford Hospital and Health Centers, Detroit, MI. May, 1999. Visiting Professor Indiana State University. October 1999. Visiting Professor, Department of Biology, Eastern Michigan University. November 2001. Visiting Professor, Department of Biological Sciences, Purdue University. December 2002. Visiting Professor, Department of Chemistry, University of Notre Dame. February 2003 Invited Speaker Chicago Cytoskeleton Meeting February 2003 Invited Symposium Speaker American Society of Nephrology Annual Meeting November 2003 Moderator American Society of Nephrology Evening Symposium Annual Meeting 2004, 2008.

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Invited Symposium Speaker American Society of Nephrology Annual Meeting November 2010.

GRANTS AND FELLOWSHIPS: Grants Awarded - Completed: Showalter Research Trust. Cellular Recovery Mechanisms Stimulated by Growth Factor Treatment in Ischemic Acute Renal Failure P.I. 7/1997-6/1998. $58,340 total costs American Heart Association (Indiana Affiliate) Postdoctoral Fellowship (Narayan Raman). P.I./Mentor. 7/98-6/2000. $41,000. NIH NRSA Postdoctoral Fellowship (Tim Sutton). P.I./Scientific Mentor. 7/1998-6/00 $37,012 per annum direct costs. NIH/NIDDK R01 (Atkinson, S.J., Principal Investigator) Rho family GTPases and actin function in renal ischemia. 7/1/99-06/30/04. Annual direct costs: $135,426; Annual total costs: $202,462; Direct costs entire period: $718,996; Total costs entire period: $1,072,679. 35% effort NIH/NIDDK P01 (Molitoris, B.A., P.I.) 01/01/00-12/31/04 NIH/NIDDK Annual Direct Costs $626,896 Actin Dysregulation in Ischemia: Mechanisms and Effects. Co-P.I. projects 1 and 2. 25% effort. NIH/NHLBI P01 (Skalnik, D., P.I.). 04/01/02-03/31/07 Role of Rac2 in development and function of blood cells $1,411,862 Annual Direct Costs. Director, Core B (Imaging). 10% effort. NIH/NIDDK (Molitoris, B.A., P.I.) George OBrien Center for Advanced Renal Microscopy. 06/01/02-05/31/07 $1,042,006 annual direct costs. P.I Project 2 (Phagocyte Mediators of Ischemia-Reperfusion Injury). 10% effort. NIH/NIDDK (Dagher, P.C., P.I.) Role of guanine nucleotides in ischemic renal injury. Consultant, 5% effort. NIH/NIDDK (Dunn, K., P.I.) Regulation of endocytosis in cultured renal epithelia. Consultant, 5% effort. NIH/NIDDK (Clapp, D.W., P.I.) Neurofibromatosis type I regulates myelopoiesis. Consultant, 5% effort. Eli Lilly & Co. (Molitoris, B.A., P.I.) Intravital Fluorescence Microscopy for Drug Discovery. 2005-2006. P.I. Sub-project Translocation of GLUT4 in skeletal muscle in vivo. 10% Effort.

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NIH/Office of the Director R56 Directors Bridge Award (Atkinson, S.J., P.I.) Rho GTPases and Actin Function in Renal Ischemia. 4/2007-5/2008. Total direct costs $250,000, Total direct and indirect costs $327,000. 45% Effort. Grants Awarded Current NIH/NIDDK R01 (Atkinson S.J., P.I.) Rho GTPases and Actin Function in Renal Ischemia. 5/2008-4/2013. Annual Direct Costs $250,000. Total 5 year direct and indirect costs $1,882,028. 45% Effort. NIH/NIDDK P30 (Molitoris, B.A., P.I.) George OBrien Center: Center for Advanced Renal Microscopy and Analysis. 8/3/2007-5/31/2012. P.I. Education Core. 15% Effort. Quark Pharma (Atkinson S.J., P.I.). Role of RTP801 in Cytoskeletal Organization. 20062009 Total direct costs ($30,756) direct and indirect costs $38,256. 10% Effort. Quark Pharma (Molitoris B.A., P.I.). Kidney siRNA Therapeutics. 10% effort. NIH/NHLBI R01 (Dinauer, M.C., P.I.). Phagocyte NADPH Oxidase. 6/1/2007-5/30/2012. Collaborator. 5% Effort. NIH/NHLBI R01 (Dagher, P.C., P.I). Role of TLR Signaling in Sepsis. 3/1/2009-2/28/2014. Collaborator, 5% Effort. Grants Submitted: NIH/NIDDK (Atkinson, S.J., Molitoris, B.A. Dual P.I.s) Prevention of Toxic Acute Kidney Injury Using siRNA. Submitted 10/2009. Award expected 2011 (6th percentile). PUBLICATIONS: Original Research Articles (Peer-Reviewed) 1. Atkinson, S.J. and Stewart, M. (1991). Expression in Escherichia coli of Fragments of the Coiled-Coil Rod Domain of Rabbit Myosin: Influence of Different Regions of the Molecule on Aggregation and Paracrystal Formation. J.Cell Sci. 99: 823-836. 2. Atkinson, S.J. and Stewart, M. (1992). Molecular Interactions in Myosin Assembly. Role of the 28-Residue Charge Repeat in the Rod. J.Molec. Biol. 226: 7-13. 3. Machesky, L.M., Atkinson, S.J., Ampe, C., Vandekerckhove, J. and Pollard, T.D. (1994). A Cortical Complex of Seven Acanthamoeba Polypeptides Including Two Unconventional Actins Binds to Profilin. J.Cell Biol. 127: 107-115. 4. Kelleher, J.F., Atkinson, S.J. and Pollard, T.D. (1995). Sequences, Structural Models, and Cellular Localization of the Actin-related Proteins Arp2 and Arp3 from Acanthamoeba. J. Cell Biol. 131: 385-397.

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5. Gopalakrishnan, S., Raman, N., Atkinson, S.J. and Marrs, J.A. (1998). Rho GTPase Signaling Regulates Tight Junction Assembly and Protects Tight Junctions During ATP Depletion. Am. J. Physiol.275 (Cell Physiol.): C798-809. 6. Pavalko, F.M., Chen, N.X., Turner, C.H., Burr, D.B., Atkinson, S.J., Hsieh, Y-F, Qiu, J. and Duncan, R.L. (1998). Fluid shear-induced mechanical signaling in MC3T3-E1 osteoblasts requires cytoskeleton-integrin interactions. Am. J. Physiol. (Cell Physiol.). 275: C1591-C1601. 7. Schwartz, N., Hosford, M., Sandoval, R., Wagner, M.C., Atkinson, S.J., Bamburg, J. and Molitoris, B.A. (1999) Ischemia Activates Actin Depolymerizing Factor: Role in Proximal Tubule Microvillar Actin Alterations. Am. J. Physiol. (Renal Physiol.) 276: F544-551. 8. Roberts, A.W., Kim, C., Zhen, L., Kapur, R., Petryniak, B., Pollock, J., Lowe, J.B., Atkinson, S.J., Dinauer, M.C. and Williams, D.A. (1999) Rac2 Deficiency in vivo is Characterized by Abnormalities in Neutrophil Function and Host Defense. Immunity 10: 183-196. 9. Raman, N. and Atkinson, S.J. (1999). Role of the p21 GTPase Rho in Remodeling of the Actin Cytoskeleton in an Animal Cell Culture Model of Renal Ischemia. Am J. Physiol (Cell Physiol.). 276: C1312-1324. 10. Zong, H., Raman, N., Mickelson-Young, L.A., Atkinson, S.J. and Quilliam, L.A. (1999) Loop 6 of RhoA Confers Specificity for Effector Binding, Stress Fiber Formation and Cellular Transformation. J. Biol. Chem. 274: 4551-4560. 11. Mehta, D., Tang, D.C., Atkinson, S.J., and S.J. Gunst (2000). Role of Rho in calciuminsensitive contraction and paxillin tyrosine phosphorylation in smooth muscle. Am. J. Physiol.: (Cell Physiol.). 279: C308-C318 12. Williams, D.A., Tao, W., Yang, F-C., Kim, C., Gu, Y., Mansfield, P., Levine, J.E., Petryniak, B., Derrow, C.W., Harris, C., Jia, B., Zheng, Y., Ambruso, D.R., Lowe, J.B., Atkinson, S.J., Dinauer, M.C., and Boxer, L. (2000). Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency. Blood. 96:1646-1654 13. Yang, F, Atkinson, S.J.., Gu, Y., Borneo, J.B., Roberts, A.W., Pennington, J. & Williams, D.A. Rac2, a Rho-related guanine triphosphatase, is critical for in vivo hematopoietic stem cell adhesion and migration Proc. Natl. Acad. Sci. USA 98: 5614-5618 ( denotes joint first author). 14. Sutton, T.A., Mang H.E. and S.J.Atkinson (2001). Rho-Kinase Regulates Myosin II Activation during Recovery Following ATP depletion. Am. J. Physiol. (Renal Physiol.) 281: F810-818 15. Insall, R.H, Mueller-Taubenberger, A., Machesky, L.M., Koehler, J., Simmeth, E., Atkinson, S.J., Weber, I. and G. Gerisch (2001). Dynamics of the Dictyostelium Arp2/3

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Complex in Endocytosis, Cytokinesis, and Chemotaxis Cell Motil. Cytoskel. 50: 115-128 16. Herget-Rosenthal, S., Hosford, M., Kribben, A., Atkinson, S.J., Sandoval, R.M. & B.A.Molitoris (2001). Characteristics of EYFP-actin and visualization of actin dynamics during ATP depletion and repletion. Am. J. Physiol. (Cell Physiol.) .281: C1858-1870 17. Jin, Y, Atkinson, S.J., Marrs, J.A. and Gallagher, P.J. (2001). Myosin II light chain phosphorylation regulates membrane localization and apoptotic signaling of tumor necrosis factor receptor-1. J. Biol. Chem. 276: 30342-30349 18. Tao, W., Bailey, J.R., Atkinson, S.J., Connors, B., Evan, A., Yoder, M. & Williams, D.A. (2002) The TRQQKRP motif located near the C-terminus of Rac2 is essential for its biological functions and intracellular localization. Blood, 100: 1679-1688. 19. Dunn, K.W., R.M. Sandoval, K.J. Kelly, P. Dagher, G. Tanner, S.J. Atkinson, R.L. Bacallao and B.A. Molitoris. (2002) Functional studies of the kidney of living animals using multicolor 2-photon microscopy. Am. J. Physiol. (Cell Physiol.) 283: C905-C916 20. Price, M.O., Atkinson, S.J. Knaus, U.G. & M.C. Dinauer (2002) Rac Activation Induces Phagocyte NADPH Oxidase Activity in Cells and Level of Superoxide Production is Exchange Factor-Dependent J. Biol. Chem. 277: 19220-19228 21. Hallett, M.A., Dagher, P.C. & S.J. Atkinson. (2003). Rho GTPases show differential sensitivity to nucleotide triphosphate depletion in a model of ischemic cell injury. Am. J. Physiol. (Cell Physiol.) 285: C129-C138 22. Gopalakrishnan, S., Hallett, M.A., Atkinson, S.J. & J.A. Marrs. (2003). Differential regulation of Rho signaling pathways in renal epithelial cell lines. Am. J. Physiol. (Cell Physiol.). 285: C102-C111 23. Gu, Y., Filippi, M.-D., Cancelas, J.A., Siefring, J.E., Williams, E.P., Jasti, A.C., Harris, C. E., Lee, A. W., Prabhakar, R., Atkinson, S.J., Kwiatkowski, D.J. & David A. Williams. (2003). Hematopoietic Cell Regulation by Rac1 and Rac2 Guanosine Triphosphatases. Science 302: 445-449. 24. Ingram, D.A., Yang, F.-C., Chen, S., Ratner, N., Monk, K.R., Clegg, T., White, H., Mead, L., Wenning, M.-J., Williams, D.A., Kapur, R., Atkinson, S.J. & Clapp, D. W. (2003) Neurofibromin-deficient Schwann cells secrete a potent stimulus for Nf1 +/- mast cells J. Clin. Invest. 112: 1851-1861. 25. Atkinson, S.J., Hosford, M., and Molitoris, B.A. (2004). Mechanism of Actin Polymerization in Cellular ATP Depletion J. Biol. Chem..279: 5194-5199 26. Yamauchi, A., Marchal, C., Kim, C, Towe, J.A., Atkinson, S.J. & M.C. Dinauer (2004) Rac2-deficient macrophages have selective defects in superoxide production and phagocytosis of opsonized particles. J. Immunol. 173: 5971-5979

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27. Yamauchi, A., Marchal, C., Molitoris, J., Pech, N., Knaus, U., Towe, J., Atkinson, S.J. & M.C. Dinauer. (2005). Rac GTPase isoform-specific regulation of NADPH oxidase and chemotaxis in murine neutrophils in vivo: Role of the C-terminal polybasic domain. J. Biol. Chem., 280: 953-964. (Published online 10/25/04 as doi:10:1074/jbc.M408820200) 28. Dirk Carstanjen, Akira Yamauchi, Annemart Koornneef, Heesuk Zang, Marie-Dominique Filippi, Chad Harris, Jason Towe, Simon Atkinson, Yi Zheng, Mary C. Dinauer, and David A. Williams. Rac2 Regulates Neutrophil Chemotaxis, Superoxide Production and Myeloid Colony Formation through Multiple Distinct Effector Pathways. J. Immunol. 174(8): 4613-20 29. Ransom, R. F., Lam, N.G., Hallett, M.A., Atkinson, S.J. and W.E. Smoyer (2005) Glucocorticoids protect and enhance recovery of cultured murine podocytes via actin filament stabilization. Kidney International. 68: 2473-83. 30. Suh CI, Stull ND, Li XJ, Tian W, Price MO, Grinstein S, Yaffe MB, Atkinson S, Dinauer MC. The phosphoinositide-binding protein p40phox activates the NADPH oxidase during Fcgamma IIA receptor-induced phagocytosis. J Exp Med. 2006 Aug 7;203(8):1915-25. 31. Szczur K, Xu H, Atkinson S, Zheng Y, Filippi MD. Rho GTPase CDC42 regulates directionality and random movement via distinct MAPK pathways in neutrophils. Blood. 2006 08(13):4205-13. Epub 2006 Aug 24. 32. Gopalakrishnan S, Hallett MA, Atkinson S, Marrs JA. aPKC-Par complex dysfunction and tight junction disassembly in renal epithelial cells during ATP-depletion. Am J Physiol Cell Physiol. 2007 Mar; 292(3):C1094-102. Epub 2006 Aug 23. 33. Basu S, Ray NT, Atkinson SJ, Broxmeyer HE. Protein phosphatase 2A plays an important role in stromal cell-derived factor-1/CXC chemokine ligand 12-mediated migration and adhesion of CD34+ cells. J Immunol. 2007 Sep 1;179(5):3075-85. 34. Wei Tian, Xingjun Li, Wenyu Ming, Natalie D. Stull, Chang-Il Suh, Sergio Grinstein, Simon Atkinson, and Mary C. Dinauer FcR-stimulated activation of the NADPH oxidase: Phosphoinositide binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome. Blood. 2008 Nov 1;112(9):3867-77. Epub 2008 Aug 18. 35. Jayme D. Allen, Zahara M. Jaffer, Su-Jung Park, Sarah Burgin, Clemens Hofmann, Mary Ann Sells, Shi Chen, Ethel Derr-Yellin, Elizabeth G. Michels, Waylan K. Bessler, David A. Ingram, Simon J. Atkinson, Jeffrey B. Travers, Jonathan Chernoff and D. Wade Clapp. P21activated kinase regulates mast cell degranulation via effects on calcium mobilization and cytoskeletal dynamics. Blood. 2009 Jan 5. [Epub ahead of print]. 36. Silvia B. Campos, Sharon L. Ashworth, Sarah Wean, Melanie Hosford, Ruben M. Sandoval, Mark A. Hallett, Simon J. Atkinson & Bruce A. Molitoris. Cytokine induced Factin reorganization in endothelial cells involves RhoA activation. Am J. Physiol. (Renal). 2009 Mar;296(3):F487-95. Epub 2009 Jan 14.

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37. Li XJ, Tian W, Stull ND, Grinstein S, Atkinson S, Dinauer MC. A fluorescently tagged C-terminal fragment of p47phox detects NADPH oxidase dynamics during phagocytosis. Mol Biol Cell. 2009 Mar;20(5):1520-32. Epub 2009 Jan 7. 38. Molitoris, BA, Dagher, PC, Sandoval, RM, Campos, SB, Ashush, H, Freidman, E, Brafman, A, Faerman, A, Atkinson, SJ, Thompson, J., Kalinski, H, Skaliter, R, Erlich, S & E. Feinstein. siRNA Targeting p53 Minimizes Ischemia and Cisplatin-induced Acute Kidney Injury. J. Am. Soc. Nephrol. 2009 Aug;20(8):1754-64. Epub 2009 May 21. 39. Chen NX, Chen X, O'Neill KD, Atkinson SJ, Moe SM. RhoA/Rho kinase (ROCK) alters fetuin-A uptake and regulates calcification in bovine vascular smooth muscle cells (BVSMC). Am J Physiol Renal Physiol. 2010 Sep;299(3):F674-80. Epub 2010 Jul 7. 40. Deqiang Li, Mark A Hallett, Wuqiang Zhu, Michael Rubart-von der Lohr, Ying Liu, Zhenyun Yang, Hanying Chen, Laura S Haneline, Rebecca J Chan, Robert J Schwartz, Loren J Field, Simon J Atkinson, and Weinian Shou. Dishevelled associated activator of morphogenesis 1 (DAAM1) is required for heart morphogenesis. Development. In press. Submitted Manuscripts Hao Zhang Hallett, MA and Simon J. Atkinson. Cortactin localization and Src-dependent tyrosine phosphorylation are altered in a cell culture model of renal ischemia. Am. J. Physiol (Renal). Submitted.

Reviews and Chapters (Not Peer-Reviewed) 1. Atkinson, S.J. and Stewart, M. (1991) Molecular Basis of Myosin Assembly: CoiledCoil Interactions and the Role of Charge Periodicities J. Cell Sci. Suppl. 14: 7-10. 2. Atkinson, S.J., Doberstein, S.K. and Pollard, T.P. (1992). Moving off the Beaten Track Curr. Biol. 2: 326-328. 3. Molitoris, B.A. and Atkinson, S.J. (2001) Cytoskeletal Alterations as a Basis of Cellular Injury in Acute Renal Failure in Acute Renal Failure: A Companion to Brenner and Rectors The Kidney Molitoris, B.A. and Finn, W.F., Eds. Philadelphia: W.B.Saunders and Co. 4. Atkinson, S.J. (2006) Functional Intravital Imaging of Leukocytes in Animal Models of Renal Injury. Nephron Physiol. 103: 86-90. Abstracts (since 1994): 1. Atkinson, S.J. and Pollard, T.D. (1994) Profilin-associated Unconventional Actins

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from Dictyostelium discoideum. American Society for Cell Biology 34th Annual Meeting. 2. Kelleher, J.F., Atkinson, S.J. and Pollard, T.D. (1994) Characterization of ActinRelated Proteins in Profilin Binding Complex from Acanthamoeba. American Society for Cell Biology 34th Annual Meeting. 3. Molitoris, B.A., Atkinson, S.J., Hosford, M., Sandoval, R. (1996) Mechanism of Cellular ATP depletion-induced actin polymerization. American Society for Cell Biology 36th Annual Meeting. 4. Atkinson, S.J. and Raman, N. (1997) The mechanism of ischemia-induced actin cytoskeletal disruption in proximal tubule epithelium involves the small GTPase Rho American Society of Nephrology Annual Meeting. 5. Marrs, J.A., Raman, N., Mokanyk, W.T., Atkinson, S.J. and Gopalakrishnan, S. (1997) Regulation of Renal Epithelium Tight Junction Assembly by Rho-GTPase Signaling and Ischemia. American Society of Nephrology Annual Meeting. 6. Raman, N, Mokanyk, W.T, and Atkinson, S.J. (1997) Rho activity is required for maintenance of actin stress fibers in proximal tubule epithelial cells (LLC-PK) during conditions of ATP-depletion. American Society for Cell Biology 37th Annual Meeting. 7. Gopalakrishnan, S., Raman, N, Atkinson, S.J. and Marrs, J.A. (1997) Regulation of tight junction assembly by rho-GTPase signaling and ischemia. American Society for Cell Biology 37th Annual Meeting. 8. Pavalko, F.M., Chen, N.X., Turner, C.H., Burr, D.B., Atkinson, S., Hsieh, Y.F., Qui, J, and Duncan, R.L. (1998) Cytoskeletal-integrin interactions are required for fluid shearinduced mechanical signaling in MC-3T3-E1 Osteoblasts. American Society for Cell Biology 38th Annual Meeting 9. Raman, N., Zong, H, Quilliam, L.A., and Atkinson, S.J. (1998) A rac-dependent pathway for stress fiber formation in epithelial cells. American Society for Cell Biology 38th Annual Meeting 10. Sutton, T., Poteat, B., Marrs, J.A., and Atkinson, S.J., (1998) Overexpression of wildtype and kinase-dead myosin light chain kinase alters the actin cytoskeleton in MDCK cells. American Society for Cell Biology 38th Annual Meeting 11. Atkinson, S.J., Roberts, A, Dinauer, M.C., and Williams, D.A.. (1998) Neutrophils from rac2 knockout mice are defective in chemotaxis, motility and actin regulation. American Society for Cell Biology 38th Annual Meeting 12. Gopalakrishnan, S., Raman, N., Atkinson, S.J., and Marrs, J.A. (1998) Regulation of adherens junction assembly by rho signaling in normal and ischemic epithelial cells. American Society for Cell Biology 38th Annual Meeting 13. Raman, N., and Atkinson, S.J. (1998). Ischemia-induced actin cytoskeletal disruption

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in proximal tubule epithelium is due to inactivation of p21-rho GTPase. American Society of Nephrology Annual Meeting. 14. Raman, N., Gosch, A., Gopalakrishnan, S., Marrs, J.A., & Atkinson, S.J. (1999) Association of vinculin with E-cadherin adhesion complex is modulated by small GTP binding protein Rho American Society for Cell Biology 39th Annual Meeting 15. Atkinson, S.J., Yang, F., & Williams, D.A. (1999) Consequences of Rac2 Deficiency for Mast Cell and Neutrophil Function. American Society for Cell Biology 39th Annual Meeting. 16. Jin, Y., Atkinson, S. J., Marrs, J. A., Poteat, B.A., & Gallagher, P.J. (1999) Tumor necrosis factor induced apoptosis is regulated by myosin light chain phosphorylation. American Society for Cell Biology 39th Annual Meeting. 17. Sutton, T.A., Mang, H.E. & Atkinson, S.J. Alteration of non-muscle myosin II distribution in renal epithelial cells during ATP depletion and repletion. American Society of Nephrology 33rd Annual Meeting, Toronto, ON. 18. Molitoris, B.A., Atkinson, S.J. & Hosford, M. Cellular ATP depletion results in reduced ATP-G-actin. American Society for Cell Biology 40th Annual Meeting. 19. Hallett, M.A. & Atkinson, S.J. Measurement of Rho activity in renal ischemia during ATP depletion and recovery American Society for Cell Biology 40th Annual Meeting. 20. Atkinson, S.J., Yang, Fengchun, Pennington, J., & Williams, D.A. Contrasting cell type-specific phenotypes of murine rac2 deficiency. American Society for Cell Biology 40th Annual Meeting. 21. F.C. Yang, D.Carstanjen, S.J. Atkinson, J.B. Borneo, C.E. Harris, K. Rufatto, D.A. Williams. The Rho GTPase, Rac2, is a critical regulator of dendritic cell function. American Society of Hematology Annual Meeting 2001. 22. Hallett, M.A. & S.J. Atkinson. Cdc42 Activity and Cortactin Colocalization in Ischemia. American Society for Cell Biology 41st Annual Meeting 2001. 23. Hallett, M.A. & S.J. Atkinson. Rho GTPases mediate cytoskeletal disruption and reassembly in ATP depletion and recovery. American Society of Nephrology 35th Annual Meeting, Philadelphia, PA 2002 24. Kelly, K.J., Dinauer, M.C. & S.J. Atkinson. Ischemia-reperfusion injury is markedly attenuated in mice lacking the hematopoietic-specific Rho GTPase Rac2. American Society of Nephrology 35th Annual Meeting, Philadelphia, PA 2002. 25. C-terminal polybasic domain of rac mediates isoform-specific regulation of chemotaxis and NADPH oxidase activity in murine neutrophils in vivo. Yamauchi, A., Marchal, C., Molitoris, J., Pech, N., Knaus, U., Towe, J., Atkinson, S.J. & M.C. Dinauer. (2004). American Society for Cell Biology Annual Meeting Washington DC 2004

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26. Gopalakrishnan, S., Hallett MA., Atkinson, S.J., and Marrs, J.A. Dysregulation of aPKC signaling pathway and tight junction assemply during ischemic renal epithelial cell injury. American Society for Cell Biology Annual Meeting Washington DC 2004 27. Silvia B. Campos, Melanie Hosford, Ruben M. Sandoval, Mark A. Hallett, Simon J. Atkinson, Bruce A. Molitoris. Cytokine-Induced F-Actin Formation and Reorganization in MS-1 Endothelial Cells Involves RhoA Activation. American Society of Nephrology Annual Meeting, St. Louis, MO 2004. 28. Marie-Dominique Filippi, Haiming Xu, Jason Towe, Chad E. Harris, Kathleen Szczur, Lei Wang, Simon Atkinson, Yi Zheng, David A. Williams. The Rho GTPase CDC42 Regulator CDC42GAP Plays a Critical Role in Neutrophil Migration Via Podosome-Like Formation. American Society of Hematology Annual Meeting. San Diego 2004. 29. Chang ll Suh, Natalie Stull, Yoshito Fuji, Sergio Grinstein, Michael Yaffe, Simon Atkinson, Mary C. Dinauer. Role for p40phox in Fc-Receptor-Induced NADPH Oxidase Activation. Session Type: Oral Session. American Society for Hematology Annual Meeting. San Diego. 2004. 30 Simon J. Atkinson, NT Raye, GJ Rhodes and KJ Kelly. TLR4 and Rac2 contribute to renal injury in sepsis. American Society of Nephrology Annual Meeting, Philadelphia 2005. 31 Mark A. Hallett and S.J. Atkinson RhoB GTPase is up-regulated in the kidney following ischemia. American Society for Cell Biology Annual Meeting, San Francisco 2005. 32. Mark A. Hallett and Simon J. Atkinson. Role of mTOR and AMPK signaling in regulation of Rho GTPase activity. American Society for Nephrology Annual Meeting, San Diego. 2006. 33. Nicole T. Raye and Simon J. Atkinson. Neutrophil protease granule release is a major contributor to renal ischemia/reperfusion injury. American Society for Nephrology Annual Meeting, San Francisco. 2007. 34. Mark A. Hallett, Hao Zhang and Simon J. Atkinson. mTOR and AMPK Signaling Regulate RhoA Activity during Renal Ischemia. American Society for Cell Biology Annual Meeting, Washington DC. 2007. 35. Hao Zhang and Simon Atkinson. AMPK Signaling regulates RhoA activity during ATP depletion. American Society for Nephrology Annual Meeting, Philadelphia, 2008 36. Hao Zhang and Simon Atkinson. Regulation of RhoA activity in ischemia. American Society for Cell Biology Annual Meeting. San Francisco. 2008.

CURRICULUM VITAE Martin Bard Present Position: Professor of Biology, Indiana University-Purdue University Indianapolis (IUPUI), 1992present Adjunct Professor, Indiana University School of Medicine, Biochemistry and Molecular Biology Department, 1992-present Education: B.S. City College of New York, 1965 Ph.D. University of California (Berkeley), 1971 Professional Experience: Post-Doctoral Fellow, University of Sheffield, 1971-1973 Post-Doctoral Fellow, State University of New York (Albany), 1973-1974 Post-Doctoral Fellow, University of California (Santa Cruz), 1974-1975 Assistant Professor of Biology, IUPUI, 1975-1979 Associate Professor of Biology, IUPUI, 1980-1992 Sabbatical Leave, Eli Lilly & Co., 1982-1983 Sabbatical Leave, IUSM, Biochemistry and Molecular Biology Department, 2006 Professional Societies: American Association for the Advancement of Science American Society for Microbiology Genetics Society of America Grants: NSF: $459,451 2006-2011 (PI, Robin Wright, Co-PI Martin Bard) Subcontract: $98, 181 Cold Adaptation in Yeast: the Role of ERAD Associated Degradation and Sterol Metabolism in Yeast NIH: R01 GM62104; $893, 000, 2004-2009 The Yeast Ergosterol Pathway Burroughs Wellcome Fund; $425,000 1999-2004 Characterization of New Target sites for Antifungal Intervention in the Candida albicans Ergosterol Pathway

NIH: R01 GM62104; $750,000, 2000-2004 The Yeast Ergosterol Pathway NIH: PHS R01 AI38598-01; $ 706,500 1995-2000 Yeast Ergosterol pathway-New Antifungal Target Sites Predoctoral fellowship from American Heart Association for Matthew Kennedy.(July 1998-June 2000) and Beth Skaggs (July 1994-June1996) US Army; $218,836 1995-1999 Determining Antifungal Target Sites in the Sterol pathway of the Yeasts Candida and Saccharomyces Johnson & Johnson Focused Giving Award; $150,000 1992-1994 Cloning of Yeast Sterol Genes for the Purpose of Identifying New Targets for Antifungal Drugs NIH; PHS R15 AG11116-01; Small Instrumentation Grant $10887, 1992 NIH; PHS R15 GM 45959; $113,876, 1991-1994 Seeking the Yeast Sparking Sterol American Cyanamid; $26,874, 1991-1992 Cloning the Yeast delta 14 Sterol Reductase Gene NIH:PHS IR01 GM 43609; $304,000 (Jarrett & Bard, Co PIs) 1989-1992 Eli Lilly and Company; $19,800, 1988-1991 Physiological Analyses of Antibiotic Resistant Sterol Mutants of Yeast NIH: PHS R15 GM37449; $59,137. 1986-1988 Cloning and Disruption of the Yeast Sterol Transmethylase Gene Merck, Sharp & Dohme Institute for Therapeutic Research; $5,150, 1983-1986 Isolation of HMG-CoA Reductase Mutants in Yeast American Heart Association, Grant-in-Aid; $7,500. 1978-1980 Isolation of HMG-CoA Reductase Mutants in Yeast Squibb Institute for Medical Research; $1,500. 1978-1979 Physiological Analyses of Antibiotic Resistant Sterol Mutants of Yeast Internal Funding

IUPUI Signature Center for Biomembrane Sciences, PI, R. Minto; co-PIs B. Blacklock, S. Randall, M. Bard, M. Goebl, High Value Lipids from Non-Fermentable Feedstocks for a Biorenewable Economy, 2010, $90000. RSFG, Protein Interactions between Two Enzymes in Yeast Sterol Biosynthesis, 2009 $35000 Purdue Research Fund International Travel Grant, June 2007 $500 Purdue Research Foundation Award, 1992-1994 $20125 Cloning and disruption of the Yeast SCP2 Gene (Pre-doctoral Fellowship to Beth Skaggs) IUPUI School of Science Interdepartmental Grant, 1986 Indiana University Biomedical Research Support Grant, 1975, 1986 IUPUI PDP Grants, 1981, 1982, 1983 Indiana University Research Operations Committee Grant 1978, 1983 Purdue University XL Grant, 1979, 1980 Isolation of Yeast HMG-CoA Reductase Mutants IUPUI Summer Faculty Fellowship Grants to Students Indiana Affiliate American Heart Association Pre-doctoral Fellowships Beth Arthington-Skaggs 1994-1996 Mathew Kennedy 1998-2000 Patents: Bard, M. and Ingolia, T. D. Novel DNA for Expression of Delta-Aminolevulinic acid Synthetase and Related Method. No. 4,902,620, Feb. 20, 1990 Lai, MH, Kirsch, DR. and Bard, M. A DNA Sequence Encoding Sterol D14 Reductase No. 5,512,472, April 30, 1996 Lai, MH, Kirsch, DR. and Bard, M. A DNA Sequence Encoding Sterol D14 Reductase No. 5,525,496, June 11, 1996 Lai, MH, Kirsch, DR. and Bard, M. Sterol 14 Reductase Screen. No. 5,591,576, Jan. 7, 1997 Bard, M. Novel DNA Encoding Sterol Transmethylase. No. 6, 225,075. May 1, 2001

Awards: NIH Training Grant recipient-U.C. Berkeley IUPUI School of Science Research Award, 2004 Mycology Scholar Award, Burroughs Wellcome Fund, 1999-2004 Teaching Enhancement Recognition Award, IUPUI 1999 Consulting: Vitamins Inc. 1982 Miller Brewing Company, 1999 Taro Pharmaceuticals, 2002 Integrated BioScience Solutions, LLC, 2008 Full Refereed Publications: Bard, M., Biochemical and Genetic Aspects of Nystatin Resistance in Saccharomyces cerevisiae, J. Bacteriol. 111: 649-657, 1972. Bard, M., Woods, R. A. and Haslam, J. M., Porphyrin Mutants of Saccharomyces cerevisiae. Correlated Lesions in Sterol and Fatty Acid Biosynthesis. Biochem. Biophys. Res. Comm. 56: 324-330, 1974. Woods, R. A., Bard, M., Jackson, I. E. and Drutz, D. J., Resistance to Polyene Antibiotics and Correlated Sterol Changes in Two Isolates of Candida tropicalis from a Patient with an Amphotericin B Resistant Funguria, J. Infect. Dis. 129: 53-58, 1974. Woods, R. A., Bard, M., Molzahn, S. W. and Gardner, I. E., Investigations into the Biosynthesis of 24(28)-dehydroergosterol in Saccharomyces cerevisiae, Microbios. 10A: 73-80, 1974. Barton, D. H. R., Corrie, J. E. T., Widdowson, D. A., Bard, M. and Woods, R. A., Biosynthesis of Terpenes and Steroids. Part IX. The Sterols of Some Mutant Yeasts and Their Relationship to the Biosynthesis of Ergosterol, J. Chem. Soc. Perkins Trans. I. No. 11: 1326-1333, 1974. Barton, D. H. R., Corrie, J. E. T., Widdowson, D. A., Bard, M., and Woods, R. A., Biosynthetic Implications of the Sterol Content of Ergosterol-Deficient Mutants of Yeast. J. Chem. Soc. 1: 30-31, 1974. Barton, D. H. R., Gunatilaka, A. A. L., Jarman, T. R., Widdowson, D. A., Bard, M. and Woods, R. A., Biosynthesis of Terpenes and Steroids. Part X. The Sterols of Some Yeast Mutants Double Defective in Ergosterol Biosynthesis, J. Chem. Soc. Perkins Trans. I. No. 1: 88-92, 1975.

Bard, M., Woods, R. A., Barton, D. H. R., Corrie, J. E. T. and Widdowson, D. A., Sterol Mutants of Saccharomyces cerevisiae: Chromatographic Analyses, Lipids 12: 645-654, 1977. Bard, M., Wilson, K. J. and Thompson, R. M., Isolation of Sterol Mutants in Chlamydomonas reinhardi: Chromatographic Analyses, Lipids 13: 533-539, 1978. Bard, M., Lees, N. D. and Kleinhans, F. W., Differences in Crystal Violet Uptake and Cation Induced Death in Yeast Sterol Mutants, J. Bacteriol. 135: 1146-1148, 1978. Kleinhans, F. W., Lees, N. D., Bard, M., Haak, R. A. and Woods, R. A., ESR Determinations of Membrane Permeability in a Yeast Sterol Mutant, Chem. Phys. Lipids 23: 143-154, 1979. Lees, N. D. Bard, M., Kemple, M., Haak, R. A. and Kleinhans, F. W., Membrane Order in Yeast Sterol Mutants, Biochim Biophys. Acta. 553: 469-475, 1979. Lees, N. D., Lofton, S. L., Woods, R. A. and Bard, M., The Effects of Varied Energy Source and Detergent on the Growth of Sterol Mutants of Saccharomyces cerevisiae, J. Gen. Microbiol. 118: 209-214, 1980. Bard, M., Neuhauser, J. L. and Lees, N. D., Caffeine Resistance in Saccharomyces cerevisiae, J. Bacteriol. 141: 999-1002, 1980. Downing, J. F., Burrows, L. S. and Bard, M., The Isolation of Two Mutants of Saccharomyces cerevisiae which Demonstrate Increased Activity of 3-Hydroxy3-Methylglutaryl CoA Reductase, Biochem. Biophys. Res. Comm. 94: 974-979, 1980. Bard, M. and Downing, J. F., Genetic and Biochemical Aspects of Yeast Sterol Regulation Involving 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase, J. Gen. Microbiol. 125: 415-420, 1981. Bard, M. and Ingolia, T. D., Plasmid Mediated Complementation of a DeltaAminolevulinic Acid Requiring Yeast Mutant, Gene 28: 195-199, 1984. Lees, N. D., Kemple, M. D., Barbuch, R. J., Smith, M. A. and Bard, M., Differences in Membrane Order Parameter and Antibiotic Sensitivity in Ergosterol-producing Strains of Saccharomyces cerevisiae, Biochim. et Biophys. Acta. 776: 105-112, 1984. Bard, M., Lees, N. D., Barbuch, R. J., and Sanglard, D. Characterization of a Cytochrome P450 Deficient Mutant of Candida albicans. Biochem. Biophys. Res. Comm. 147: 794-800 1987.

Bard, M., Lees, N. D., Burnett, A. S. and Parker, R. A. Isolation and Characterization of Mevinolin Resistant Mutants of Saccharomyces cerevisiae. J. Gen Microbiol., 134: 1071-1078, 1988. Bard, M., Albrecht, M. R., Gupta, N., Guynn, C. J. and Stillwell, W.. Geraniol Interferes with Membrane Functions in Strains of Candida and Saccharomyces. Lipids, 23: 534-538, 1988. Lees, N.D., Kleinhans, F.W., Broughton, M.C., Pennington, D.E., Ricker, V.A. and Bard, M. Membrane Fluidity in a Cytochrome P450 - Deficient Mutant of Candida albicans. Steroids, 53: 567-578, 1989. Gaber, R.F., Copple, D.H., Kennedy, B.K., Vidal, M. and Bard, M. ERG6 is Essential for Normal Membrane Function in Saccharomyces cerevisiae but is Not Required for the Formation of the Cell Cycle "Sparking Sterol". Mol. Cell. Biol. 9: 34473456, 1989. Lees, N.D., Broughton, M.C., Sanglard, D. and Bard, M. Azole Sensitivity and Hyphal Formation of the Cytochrome P450 Deficient Mutant of Candida albicans. Antimicrob. Agents & Chemother. 34: 831-836, 1990. Goss, T.A., Bard, M., and Jarrett, H.W. High Performance Affinity Chromatography of DNA. J. Chromatog. 508: 279-287, 1990. Ashman, W.H., Barbuch, R.J., Ulbright, C.E., Jarrett, H.W. and Bard, M. Cloning and Disruption of the Yeast C-8 Sterol Isomerase Gene. Lipids 26: 628-632, 1991. Arthington, B.A., Bennett, L.G., Skatrud, P.L., Guynn, C.J., Barbuch, R.J., Ulbright, C.E., and Bard, M. Cloning, Disruption, and Sequence of the Gene Encoding the Yeast C-5 Sterol Desaturase Gene. Gene 102: 39-44, 1991. Broughton, M.C., Bard, M. and Lees, N.D. Polyene Resistance in Ergosterol-Producing Strains of Candida albicans. Mycoses 34: 75-83, 1991. Goss, T.A., Bard, M. and Jarrett, H.W. High Performance Affinity Chromotography of RNA. J. Chromatog. 588: 157-164,1991. Arthington, B.A., Hoskins, J.A., Skatrud, P.L. and Bard, M. Nucleotide Sequence of the Gene Encoding Yeast C-8 Sterol Isomerase. Gene 107: 173-174,1991. Heidepriem, R. W., Livant, P. D., Parish, E. J., Barbuch, R. J., Broaddus, M. G., and Bard, M. A Simple Method for the Isolation of Zymosterol from a Sterol Mutant of Saccharomyces cerevisiae. J. Steroid Biochem. Mol. Biol. 43: 741-743, 1992.

Bard, M., Lees, N.D., Turi, T., Craft, D., Cofrin,L., Barbuch, R., Koegel,C., and Loper, J. C. Sterol Synthesis and Viability of erg11 (Cytochrome P450 Lanosterol Demethylase) Mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28: 963-967,1993. Burden, R. S., James, C. S., Bailey, A. M., Keon, J. P. R., Croxen, R, Bard, M., and Hargreaves, J. A. Isolation and Characterization of the ERG2 Gene Encoding 87 Sterol Isomerase, from the Maize Smut Pathogen, Ustilago maydis . Experim. Mycol. 18: 87-92,1994. Lai, M. L., Bard, M., Pierson, C. A., Alexander, J. F., Goebl, M., Carter, G. T., and Kirsch, D. R. The Identification of a Gene Family in the Saccharomyces cerevisiae Ergosterol Biosynthesis Pathway. Gene. 140: 41-49, 1994. Lai, M. L., Bard, M., and Kirsch, D. R. Identifications of a New Ypt/Rab-like Monomeric G-Protein Sequence in Saccharomyces cerevisiae . Yeast. 10: 399-402, 1994. Keon, J.P.R., James, C.S., Court, S., Baden-Daintree, C., Bailey,A.M., Burden, R.S., Bard, M., and Hargreaves, J. A. Isolation of the ERG2 Gene, Encoding Sterol 8 to 7 Isomerase, from the Rice Blast Fungus Magnaporthe grisea and its Expression in the Maize Smut Pathogen, Ustilago maydis Curr. Genet. 25: 531537, 1994. Bard, M., Bruner, D.A., Pierson, C.A., Lees, N.D., Biermann, B., Frye, L., Koegel, C., and Barbuch, R. Cloning and Characterization of ERG25, the S. cerevisiae Gene Encoding C-4 Sterol Methyl Oxidase. Proc. Natl. Acad. Sci. (USA). 93: 186-190, 1996. Skaggs, B.A., Alexander, J.F., Pierson,C.A., Schweitzer, K.S., Chun,K.T., Koegel, C. Barbuch, R. and Bard, M. Cloning and Characterization of the Saccharomyces cerevisiae C-22 Sterol Desaturase Gene Encoding a Second Cytochrome P450 Involved in Ergosterol Biosynthesis. Gene. 169: 105-109, 1996 Gachotte, D., Husselstein, T., Bard, M., Lacroute, F. and Benveniste, P. Isolation and Characterization of an A. thaliana cDNA Encoding a 7-sterol -C-5-desaturase by Functional Complementation of a Defective Yeast Mutant. Plant J. 9: 391-398, 1996. Husselstein, T., Gachotte, D., Desprez, T., Bard, M., and Benveniste, P. Transformation of Saccharomyces cerevisiae with a cDNA Encoding a Sterol-C-methyl Transferase from Arabidopsis thaliana Results in the Synthesis of 24-ethyl Sterols. FEBS Lett. 381: 87-92, 1996.

Yang, H., Bard, M., Bruner, D., Gleeson, A., Deckelbaum, RJ, Aljinovic, G., Pohl,TM., Rothstein, R., and Sturley, S.L. Sterol Esterification in Yeast: A Two Gene Process. Science. 272: 1353-1356, 1996. Arthington-Skaggs, B.A., Crowell, D.N., Yang, H., Sturley, S.L., and Bard, M. Positive and Negative Regulation of a Sterol Biosynthetic Gene (ERG3) in the PostSqualene Portion of the Yeast Ergosterol Pathway. FEBS Lett. 392: 161-165. 1996. Gachotte, D., Pierson, C.A., Lees, N.D., Barbuch, R., Koegl, C., and Bard, M. A Yeast Sterol Auxotroph (erg25) is Rescued by Addition of Azole Antifungals and Reduced Levels of Heme. Proc. Natl. Acad. Sci. (USA). 94: 11173-11178, 1997. Jensen-Pergakes, K.L., Kennedy, M.A., Lees, N.D., Barbuch,R., Koegel, C., and Bard, M. Sequencing, Disruption, and Characterization of the Candida albicans Sterol methyltrasferase (ERG6) Gene: Drug Susceptibillity Studies in erg6 Mutants. Antimicrobial. Agents Chemother. 42: 1160-1167, 1998. Gachotte, D., Barbuch, R., Gaylor, J., Nickel, E. annd Bard, M. Characterization of the Saccharomyces cerevisiae ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) Involved in Sterol Biosynthesis. Proc. Natl. Acad. Sci. (USA) 95: 13794-13799, 1998. Kennedy, M.A., Barbuch, R., and Bard, M. Transcriptional Regulation of the Squalene Synthase Gene (ERG9) in the Yeast Saccharomyces cerevisiae . Biochem. Biophys. Acta 1445: 110-122, 1999. Gachotte, D., Sen, S.E., Eckstein, J., Barbuch, R., Krieger, M., Ray, B.D., and Bard, M. Characterization of the Saccharomyces cerevisiae ERG27 Gene Encoding the 3keto Reductase Involved in C-4 Sterol Demethylation. Proc. Natl. Acad. Sci. (USA) 96: 12655-12660, 1999. Kennedy, M.A., Johnson, T.A., Lees, N.D., Barbuch, R., Eckstein, J.A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 Sterol Methyl Oxidase Gene (ERG25) and Expression of an ERG25 Conditional Lethal Mutation in Saccharomyces cerevisiae. Lipids 35: 257-262, 2000. Li, X., Routt, S.M., Xie, Z., Cui, X., Fang, M., Kearns, M.A., Bard, M., Kirsch, D.A., and Bankaitis, V.A. Idenification of a Novel Family of Nonclassic Yeast Phosphatidyl Transfer Proteins Whose Function Modulates Phospholipase D Activity and Sec14p-independent Cell Growth. Mol. Biol. Cell 11: 1989-2005, 2000. Hughes, T.R., Marton, M.J., Jones, A.R., Roberts, C.J., Stoughton, R., Armour, C.D., Bennett, H.A., Coffey, E., Dai, H., He, Y.D., Kidd, M.J., King, A.M., Meyer, M.R., Slade, D., Lum, P.Y., Stepaniants, S.B., Shoemaker, D.D., Gachotte, D.,

Chakraburtty, K., Simon, J., Bard, M. and Friend, S.H. Functional Discovery via a Compendium of Expression Profiles. Cell 102: 109-126, 2000. Tinkelenberg, A.H., Liu, Y., Alcantara, F., Khan, S., Guo, Z., Bard, M. and Sturley, S.L. Mutations in Yeast ARV1 Alter Intracellular Distribution and Are Complemented by Human ARV1. J. Biol. Chem. 275: 40667-40670, 2000. Kennedy, M.A. and Bard, M. Positive and Negative Regulation of Squalene Synthase (ERG9) an Ergosterol Biosynthetic Gene in Saccharomyces cerevisiae. Biochim. Biophys. Acta. 1517: 177-189, 2001. Gachotte, D., Eckstein, J., Barbuch, R., Hughes, T., Roberts, C. and Bard, M. A Novel Gene Conserved from Yeast to Humans is Involved in Sterol Biosynthesis. J. Lipid Res. 42: 150-154, 2001. Aaron, K.A., Pierson, C.A., Lees, N.D., and Bard, M. The Candida albicans ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) is Essential for Growth. FEMS Yeast Res. 1: 93-101, 2001. Jensen-Pergakes, K., Guo, Z., Giattina, M., Sturley, S.L. and Bard, M. Transcriptional Regulation of the Two Sterol Esterification Genes in the Yeast Saccharomyces cerevisiae. J. Bacteriol. 183: 4950-4957, 2001. Darnet, S. Bard, M. and Rahier, A. Functional Identification of Sterol-4-Methyl Oxidase cDNAs from Arabidopsis thaliana by Complementation of a Yeast erg25 Mutant Lacking Sterol-4-Methyl Oxidation. FEBS Lett. 508: 39-43, 2001. Jia, N., Arthington-Skaggs, B., Lee, W., Pierson, C.A., Lees, N.D., Eckstein, J., Barbuch, R., and Bard, M. Candida albicans Sterol C-14 Reductase, Encoded by the ERG24 Gene, as a Potential Antifungal Target Site. Antimicrobial. Agents Chemother. 46: 947-957, 2002. Mo, C., Valachovic, M., Randall, S.K., Nickels, J.T., and Bard, M. Protein-Protein Interactions Among C-4 Demethylation Enzymes Involved in Yeast Sterol Biosynthesis. Proc. Natl. Acad. Sci.(USA) 99: 9739-9744, 2002. Hongay, C., Jia, N., Bard, M. and Winston, F. Mot3 Is a Transcriptional Repressor of Ergosterol Biosynthetic Genes and Is Required for Normal Vacuolar Function in Saccharomyces cerevisiae. EMBO J. 21: 4114-4124, 2002 Hand, R. A., Jia, N., Bard, M., and Craven, R.J. Saccharomyces cerevisiae Dap1p, a Novel DNA Damage Response Protein Related to the Mammalian Membrane Associated Progesterone Receptor. Euk. Cell 2: 306-317, 2003. Mo, C., Milla, P., Athenstaedt, K., Ott, R., Balliano, G., Daum, G. and Bard, M. In Yeast Sterol Biosynthesis the 3-Keto Reductase Protein (Erg27p) is Required

for Oxidosqualene Cyclase (Erg27p) Activity. Biochim. Biophys. Acta. 1663: 68-74, 2003. Lucas, M.E., Ma, Q., Cunningham, D., Peters, J., Cattanach, B., Bard, M., Elmore, B.K., and Herman, G.E. Identification of Two Novel Mutations in the Murine Nsdh1 Sterol Dehydrogenase Gene and Development of a Functional Complementation Assay in Yeast. Mol. Genet. Metabol. 80: 227-233, 2003. Lum, Y.L., Armour, C.D., Stepaiants, S.B., Cavet, G., Wolf, M.K., Butler, J.S., Hinshaw, J.C., Garnier, P., Prestwich, G.D., Leonardson, A., Garrett-Engele, P., Rush, C. M., Bard, M., Schimmack, G., Phillips, J.W., Roberts, C.J., and Shoemaker, D. D. Discovering Modes of Action for Therapeutic Compounds using a Genome-Wide Screen of Yeast Heterozygotes. Cell 116:121-137, 2004 Pierson, C.A. Eckstein, J., Barbuch, R., and Bard, M. Ergosterol Gene Expression in Wildtype and Ergosterol -Deficient Mutants of Candida albcians. Med. Mycol. 42:385-389, 2004 Tsai, H., Bard, M., Izumikawa, K., Krol, A.A., Sturm, A.M., Culbertson, N.T., Pierson, C.A., and Bennett, J. A Candida Glabrata ERG1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension. Antimicrob. Agents Chemother. 48:2483-2489, 2004 Chen, O.S. Crisp, R. J., Valachovic, M., Bard, M. Winge, D.M. and Kaplan, J. Transcription of the Yeast Iron Regulon Responds Not Directly to Iron but Rather to Iron-sulfur Cluster Biosynthesis. J. Biol. Chem. 279:29513-29518, 2004 Rogers, K.M., Pierson, C.A., Culbertson, N.T., Mo, C., Sturm, A.M., Eckstein, J., Barbuch, R., Lees, N.D., and Bard, M. Disruption of the Candida albicans CYB5 Gene Results in Increased Azole Sensitivity. Antimicrob. Agents Chemother. 48:3425-3435, 2004. Barker, K.S., Crisp, S., Wiederhold, N. Lewis, R.E., Bareither, B., Eckstein, J., Barbuch, R., Bard, M. and Rogers, P.D. Genome-wide Expression Profiling Reveals Genes Associated with Amphotericin B and Fluconazole Resistance in Experimentally Induced Antifungal Resistant Isolates of Candida albicans. J. Antimicrob. Chemother. 54:376-385, 2004 Pierson, C.A., Jia, N., Mo, C., Lees, N.D., Sturm, A.M., Eckstein, J., Barbuch, R., and Bard, M. Isolation, Characterization, and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-Keto Reductase. Med. Mycol. 42:461-473, 2004 Mo, C., Valachovic, M., and Bard, M. The ERG28 Encoded Protein, Erg28p, Interacts with Both the Sterol C-4 Demethylation Enzyme Complex as Well as the Late

Biosynthetic protein, the C-24 Sterol Methyltransferase (Erg6p). Biochim. Biophys. Acta 1686:30-36, 2004 Valachovic, N., Wilcox, L. J., Sturley, S.L., and Bard, M. A Mutation in Sphingolipid Synthesis Suppresses Defects in Yeast Ergosterol Metabolism. Lipids 39: 747752, 2004 Mallory, J.C., Crudden, G., Johnson, B.L., Mo, C. Pierson, C.A., Bard, M. and Craven, R.J. Dap1p, a Heme-Binding Protein That Regulates the Cytochrome P450 Protein Erg11p/Cyp51p in Saccharomyces cerevisiae. Mol. Cell. Biol. 25: 16691679, 2005 Bard, M., Sturm, A.M., Pierson, C.A., Brown, S., Rogers, K.M., Nabinger, S. Eckstein, J., Barbuch, R., Lees, N. D., Howell, S.A. and Hazen, K.C. Sterol Uptake in Candida glabrata: Resuce of Sterol Auxotrophic Mutants. Diagn. Microbiol. Infec. Dis. 52:285-293,2005 Mo, C. and Bard, M. Erg28p is a Key Protein in the Yeast Sterol Biosynthetic Enzyme Complex. J. Lipid Res. 46:1991-1998, 2005 Mo, C. and Bard, M. A Systematic Study of Yeast Sterol Biosynthetic Protein-protein Interactions Using the Split-Ubiquitin System, Biochim. Biophys. Acta 1737:152160, 2005 Valachovic, M., Bareither, B. M., Bhuiyan, M. S. A., Eckstein, J. Barbuch, R., Balderes, D., Wilcox, L., Sturley, S. L., Dickson, R. C. and Bard, M. Cumulative Mutations Affecting Sterol Biosynthesis In The Yeast Saccharomyces cerevisiae Result In Synthetic Lethality That Is Suppressed By Alterations In Sphingolipid Profiles. Genetics 173: 1893-1908, 2006 Loertscher, J., Larson, L.L, Matson, C.K., Parrish, M.L., Felthauser, A., Sturm, A., Tachibana, T., Bard, M. and Wright, R. Endoplasmic Reticulum Associated Degradation Is Required for Cold Adaptation and Regulation of Sterol Biosynthesis in the Yeast Saccharomyces cerevisiae, Euk. Cell 5:712-722, 2006. Rahier A, Darnet S, Bouvier F, Camara B, and Bard M. Molecular and enzymatic characterizations of novel bifunctional 3beta -hydroxysteroid-dehydrogenases/C4decarboxylases from Arabidopsis thaliana. J. Biol. Chem. 281: 27264-27277, 2006 Bhuiyan, M.S.A., Eckstein, J., Barbuch, R., and Bard, M. Synthetic Lethal Interactions Involving Loss of the Yeast ERG24-the Sterol C-14 Reductase Gene. Lipids 42:69-76, 2007

Hughes, A.L., David W. Powell, D.W., Bard, M., Eckstein, J., Barbuch, R., Link, A.J., and Espenshade, P.J. Dap1/PGRMC1 Binds and Regulates Cytochrome P450 Enzymes. Cell Metab. 5:143-149, 2007 Nakayama, H., Tanabe, K., Bard, M., Hodgson, W., Wu, S., Takemori, D., Aoyama, T., Kumaraswami, N., Metzler, L., Takano, Y., Chibana, H., and Niimi, M. The Candida glabrata Putative Sterol Transporter Gene CgAUS1 Protects Cells Against Azoles in The Presence Of Serum. J. Antimicrobiol. Chemother. 60:1264-1272, 2007 Teske, B., Taramino, S., Bhuiyan, M.S.A., Kumaraswami, N. S., Randall, S.K., Barbuch, R., Eckstein, J., Balliano, G., and Bard, M. Genetic Analyses Involving Interactions Between the Ergosterol Biosynthetic Enzymes, Lanosterol Synthase (Erg7p) and 3-Ketoreductase (Erg27p) in the Yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta. 1781:359-366, 2008 Williger, S.D., Puttikamonkul, S., Kim, K.-H., Burrtitt, J.B., Grahl, N., Metzler, L.J., Barbuch, R., Bard, M. Lawrence, C.B. and Cramer, R.A. A Sterol Regulatory Element Binding Protein Is Required for Cell Polarity, Hypoxia, Adaptation, Azole Drug Resistance, and Virulence in Aspergillus fumigatus. PLoS Pathog 4(11) 1-18 e1000200, 2008 Taramino, S., Valachovic, M., Oliaro-Bosso, S., Viola, F., Teske, B., Bard, M., and Balliano, G. Interactions of Oxidosqualene Cyclase (Erg7p) with 3Ketoreductase (Erg27p) and Other Enzymes of Sterol Biosynthesis in Yeast. Biochim. Biophys. Acta. 1801:156-162, 2010 Shakoury-Elizeh, M., Protchenko, O., Berger, A., Cox, J., Gable, K., Dunn,T.M., Prinz, W.A., Bard, M., and Philpott, C.C The Metabolic Response To Iron Deficiency In Saccharomyces cerevisiae. J. Biol. Chem. 285: 14823-14833, 2010 Taramino, S., Teske, B., Oliaro-Bosso, S., Bard, M. and Balliano, G. Divergent Interactions Involving the Oxidosqualene Cyclase and 3-Ketoreductase in the Sterol Biosynthetic Pathways of Mammals and Yeasts Biochim. Biophys. Acta. 1801:1232-1237, 2010 Nagi, M., Nakayama, H.,Tanabe, K., Bard, M., Aoyama, T., Okano, M., Higashi, S., Ueno, K., Chibana, H., Niimi, M., Yamagoe, S., Umeyama, T., Kajiwara, S., Ohno, H., and Miyazaki, Y. Transcription Factors CgUPC2A and CgUPC2B Regulate Ergosterol Biosynthetic Genes in Candida glabrata. Genes Cells, In Press

Invited Book Chapters and Review Articles

Lees, N.D., Arthington, B.L., and Bard, M. The Genetics and Molecular Biology of Genes Functioning Late in the Sterol Biosynthetic Pathway of Saccharomyces cerevisiae. p. 246-259. ACS Symposium Series "Regulation of Isopentenoid Metabolism". W. D. Nes, E. J. Parish, and J. M. Trzaskos eds. American Chemical Society, Washington, D.C. ,1992 Lees, N.D, Skaggs, B., Kirsch, D.R., and Bard, M. Cloning of the Later Genes in the Ergosterol Biosynthetic Pathway of Saccharomyces cerevisiae-A Review. Lipids 30: 221-226, 1995. Lees, N. D., Bard, M., and Kirsch, D. Biochemistry and Molecular Biology of Sterol Synthesis in Saccharomyces cerevisiae In Biochemistry and Function of Sterols (Parish, E.J. and Nes, W.D. editors) CRC Press. chap.16 pp. 85-100, 1997 Lees, N. D., Bard, M., and Kirsch, D. Biochemistry and Molecular Biology of Sterol Synthesis in Saccharomyces cerevisiae In Biochemistry and Function of Sterols Crit Rev Biochem Mol Biol. 34(1): 33-47, 1999. Daum, G., Lees, N.D., Bard, M., and Dickson, R. Biochemistry, Cell Biology, and Molecualr Biology of Lipids of Saccharomyces cerevisiae Yeast 14: 1471-1510, 1998. Lees, N.D. and Bard, M. Sterol Biochemistry and Regulation in the Yeast Saccharomyces cerevisiae. In Topics in Current Genetics 6: 213-240 (G. Daum, ed.) SpringerVerlag Heidelberg, 2003 Abstracts/Presentations/Posters: Woods, R. A., Bard, M., Jackson, I. E. and Drutz, D. J., Resistance to Polyene Antibiotics and Correlated Sterol Changes in Clinical Isolates of Candida tropicalis, J. Gen. Microbiol. 77: iii, 1973. Bard, M., Woods, R. A. and Haslam, J. M., Correlation between Defects in the Synthesis of Sterols, Unsaturated Fatty Acids, Methionine and Porphyrins in the ole Mutants of Yeast. Heredity 32: 283, 1974. Kleinhans, F. W., Bard, M., Lees, N. D. and Haak, R. A., Increased Permeability of Yeast Membrane Mutants to Ni++ as Detected by ESR, Bull. Amer. Phys. Soc. 22: 277, 1977. Kleinhans, F. W., Lees, N. D., Bard, M. and Haak, R. A., ESR Determination of Membrane Order in Yeast Sterol Mutants. Biophys J. 21: 36a, 1978. Bard, M., Wilson, K. and Thompson, R. M., Isolation of Sterol Mutants in Chlamydomonas reinhardi, Chromatographic analysis, Amer. J. Bot. 65S, 1978.

Kemple, M. D., Kleinhans, F. W., Lees, N. D. and Bard, M., EPR study of Yeast Sterol Mutants, Bull. Amer. Phys. Soc. 24: 346, 1979. Bard, M., Burnett, A. S., Burrows, L. S., and Parker, R. A. Isolation of Mevinolin Resistant Strains in Yeast. Proc. Indiana Acad. Sci. 102: 45, 1986. Bard, M., Lees, N. D., Kleinhans, F. W., Barbuch, R., and Sanglard, D. Characterization of Cytochrome P450 Deficient Mutant of Candida albicans. Proc. Indiana Acad. Sci. 103: 64, 1987. Copple, D. M., Bard, M., and Gaber, R. F. Cloning of the Yeast Sterol Methyl Transferase Gene. Proc. Indiana Acad. Sci. 103: 65, 1987. Broughton, M.C., Lees, N.D., and Bard, M. Azole Sensitivity and Hyphal Formation of a Cytochrome P450 Mutant of Candida albicans. Proc. Indiana Acad. Sci. 104: 53, 1988. Bard, M. Cloning of Ergosterol Biosynthetic Genes in Fungi. Mtg Issue-J. Amer. Oil Chemist's Society p. 428-430, 1989. Ashman, W., Bennett, L., Guynn, C., and Bard, M. Cloning of the Yeast ERG2 and ERG3 Genes. Proc. Indiana Acad. Sci. 105: 71, 1989. Bard, M., Bennett, L., and Ashman, W. Cloning of ERG2 and ERG3 from Saccharomyces. Inform 1 p. 324, 1990. Broughton, M.C., Bard, M., and Lees, N.D. Polyene Resistance in Ergosterol-producing Strains of Candida albicans. Proc. Ann. Mtg. Indiana Acad. Sci. 106: 61, 1990. Arthington, B., Bard, M. and Skatrud, P. DNA Sequence of the ERG3 Gene from Saccharomyces cerevisiae. Amer. Soc. Microbiol. Ind. Branch, 1991. Arthington, B. , Hoskins, J., Skatrud, P. and Bard, M. DNA Sequence of the ERG2 Gene from Saccharomyces cerevisiae. Amer. Soc. Microbiol. Ind. Branch, 1992. Craft, D., Turi, T., Loper, J., Barbuch, R., Koegel, C., Lees, N., and Bard, M. Saccharomyces cerevisiae erg3 Null Mutants Suppress erg11 Null Mutants to Aerobic Growth. Yeast Genetics and Molecular Biology Mtg. University of Wisconsin, Madison WI, 1993. Lai, M. L., Bard, M., Pierson, C. A., Alexander, J. F., Goebl, M., Carter, G., and Kirsch, D. R. Identification of a Gene Family in the Saccharomyces cerevisiae Ergosterol Biosynthetic Pathway via Selection for Clones Producing Morpholine Resistance. Yeast Cell Biology Mtg. , Cold Spring Harbor NY, 1993.

Alexander, J., Skaggs, B.A., Schweitzer, K.S., and Bard, M. Cloning of the Yeast ERG5 Gene. Proc.Indiana Acad. Sci. 109: 76 1993. Schweitzer, K.S., Goebl, M. and Bard, M. Characterization of the Yeast CDC41 Gene. Proc. Indiana Acad. Sci. 109: 76 1993 Skaggs, B.A. and Bard, M. Ergosterol Biosynthetic Genes in Yeast Amer. Oil Chemists Society INFORM 5: 508,1994. Skaggs, B.A. Alexander, J.F., Schweitzer, and Bard, M. Cloning of the Ergosterol Gene, ERG5. Amer. Soc. Microbiol. Indiana Branch, 1994. Skaggs, B., Yang, H.Y., Sturley, S. and Bard, M. Regulatory Analysis of the ERG3 Gene in S. cerevisiae. Amer. Soc. Microbiol. Indiana Branch, 1995. Skaggs, B. A., Crowell, D., and Bard, M. Regulatory Analysis of the ERG3 Gene in S. cerevisiae. Proc. Indiana. Acad. Sci. 111: 80, 1995. Kennedy, M.A., and Bard, M. Regulatory Analysis of the ERG9 Gene in S. cerevisiae. Proc. Indiana. Acad. Sci. 111: 79, 1995. Bard, M., Bruner, D., Pierson, C.A., Lees, N.D., Biermann, B., Frye, L., Koegel, C., and Barbuch, B. The Cloning and Characterization of ERG25, the S. cerevisiae Gene Encoding C-4 Sterol Methyl Oxidase. Proc. Indiana . Acad. Sci. 111: 87, 1995. Bard, M., Bruner, D., Pierson, C.A., Lees, N.D., Biermann, B., Frye, L., Koegel, C., and Barbuch, B. The Cloning and Characterization of ERG25, the S. cerevisiae Gene Encoding C-4 Sterol Methyl Oxidase. Yeast Cell Biol. Mtg. Cold Spring Harbor, NY, p.226, 1995. Yang, H., Bard, M., Bruner, D., Gleeson, A., Deckelbaum, R.J., Aljinovic, G., Pohl, T., Rothstein, R., and Sturley, S.L. Sterol Esterification is Mediated by Two Gene in Yeast and Regulates Sterol Biosynthesis. Circulation 92: I-496, 1995. Bard, M., Skaggs, B., Brunner,D., and Pierson, C.A. Cloning, Disruption, and Sequencing of Two New Ergosterol Biosynthetic Genes in Yeast. 87th AOCS Annual Meeting , Indianapolis ,In April, 1996. Johnson, T.A., Kennedy, M.A., Lees, N.D., and Bard,M. Cloning of the Candida albicans ERG25 Gene. Proc. Acad. Sci. 112: 86 Nov. 1996. Kennedy, M.A., Pergakes, K.J., Lees, N.D., and Bard, M. Cloning of the Candida albicans ERG6 Gene. Proc. Ind. Acad. Sci. 112: 86-87 Nov. 1996. Kennedy, M and Bard, M Regulatory Analysis of the ERG9 Gene in S. cerevisiae Proc. Indiana Acad. Sci., 113: 82 Nov. 1997.

Gachotte, D, Pierson, C.A., Lees, N.D., Barbuch, R, Koegel, C and Bard, M . A Yeast Sterol Auxotroph (erg25) is Resuced by Addition of Azole Antifungals and Reduced Levels of Heme. Johnson & Johnson Focused Giving Symposium , New Brunswick, NJ, 1997. Bard, M. Aspects of Sterol Biosynthesis in Saccharomyces Yeast Lipid Meeting 98. Smolenice, Slovakia, May,1998. Kennedy, M.A., and Bard, M. Regulatory Analysis of the ERG9 Gene in S. cerevisiae. 1998 Yeast Genetics and Molecular Biology Meeting. College Park, MD, July 1998. Gachotte, D., Gaylor, J., Nickel, E., Barbuch, R., Koegel, C., and Bard, M. Disruption and Characterization of ERG26 Encoding the 3-sterol dehydrogenase (C4methyl decarboxylase) in Saccharomyces cerevisiae . 1998 Yeast Genetics and Molecular Biology Meeting. College Park, MD, July 1998. Gachotte, D., Barbuch, R., Gaylor, J., Nickel, E. annd Bard, M. Characterization of the Saccharomyces cerevisiae ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) Involved in Sterol Biosynthesis. Johnson & Johnson Focused Giving Symposium , New Brunswick, NJ, 1998. Kennedy, M.A., Johnson,T.A., Lees,N.D., Barbuch, R., Eckstein,J.A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 Sterol Methyl Oxidase Gene (ERG25) and the Expression of ERG25 Conditional Lethal mutations in Saccharomyces cerevisiae. 90th Annual American Oil Chemists Society Mtg.: Sterol Biochemistry Section. Orlando, Fl, May, 1999. Kennedy, M.A., Barbuch, R. and Bard, M. Transcriptional Regulation of the Squalene Synthase Gene (ERG9) in the Yeast Saccharomyces cerevisiae. Gordon Research Conference:The Molecular and Cellular Biology of Lipids. Kimball Union Academy, Meriden, NH, June, 1999. Kennedy, M.A., Johnson, T.A., Lees, N.D., Barbuch, R., Eckstein, J.A., and Bard, M. Identification of the C-4 Sterol Methyl Oxidase Gene (ERG25) from Candida albicans and Expression of ERG25 Conditional lethal mutations in Saccharomyces cerevisiae. Proc. Indiana Acad. Sci. 115: 69, University of Southern Indiana , Evansville, IN, Nov. 1999 Aaron, K., Lees, N., and Bard, M. Cloning and Disruption of the ERG26 Gene in the Pathogenic Yeast Candida albicans. Proc. Indiana Acad. Sci. 115: 70, University of Southern Indiana, Evansville, IN, Nov. 1999 Gachotte, D., Sen, S.E., Eckstein, J., Barbuch, R., Krieger, M., Ray, B.D., and Bard, M.Characterization of the Saccharomyces cerevisiae ERG27 Gene encoding the

3-keto Reductase Involved in C-4 Sterol Demethylation. Johnson & Johnson Focused Giving Symposium , New Brunswick, NJ, Nov.1999. Kennedy, M.A., Johnson, T.A., Lees, N.D., Barbuch, R., Eckstein, J.A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 sterol Methyl Oxidase Gene (ERG25) and Expression of an ERG25 Conditional Lethal Mutation in Saccharomyces cerevisiae. Yeast Genetics and Molecular Biology Meeting, Seattle, WA, July, 2000. Hughes, T.R., Marton, M.J., Jones, A.R., Roberts, C.J., Shoemaker, D.D., Chakraburtty, K., Bard, M. and Friend, S.H. Functional Discovery via a Compendium of Expression Profiles. 2000 Yeast Genetics and Molecular Biology Meeting, Seattle, WA, July, 2000. Jia, N., Randall, S., Lees, N., and Bard, M. Cloning and Disruption of the ERG27 and ERG24 Genes in the Yeast Candida albicans. Proc. Indiana Acad. Sci. 116: 80,Indiana University East , Richmond, IN, Nov., 2000. Kennedy, M.A., Johnson, T.A., Lees, N.D., Barbuch, R., Eckstein, J.A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 sterol Methyl Oxidase Gene (ERG25) and Expression of an ERG25 Conditional Lethal Mutation in Saccharomyces cerevisiae. Johnson & Johnson Focused Giving Symposium , New Brunswick, NJ, Nov. 2000. Bard, M. Biosynthesis and regulation of yeast sterols. Yeast Lipid Meeting, Svendborg, Funen, Denmark, May 2001. Herman, G.E., Wang, X., Lucas, M., Ma, Q., Peters, J., Cattanach, B., Pierson, C., Bard, M., Kelley, R.I. Further Characterization of the X-linked Dominant Male Lethal Bare Patched Mutation. Amer. Soc. Human Genet. 51st Annual Meeting San Diego, CA, Oct. 2001. Mo, C., Valachovic, M., Randall, S. Nickels, J., and Bard, M. Protein-Protein interactions Among C-4 Demethylation Enzymes Involved in Yeast Sterol Biosynthesis. 2002 Yeast Genetics and Molecular Biology Meeting. University of Wisconsin, Madison, July, 2002. Hongay, C., Jia, N., Bard, M., and Winston, F. Mot3 Regulates Transcription of ERG Genes and is Necessary for the Proper function of the Endocytic Membrane Transport System. Yeast Genetics and Molecular Biology Meeting. University of Wisconsin, Madison, July, 2002. Valachovic, M., Wilcox, L., Balderes, D., Sturley, S., and Bard, M. Synthetic Lethality between Ergosterol Biosynthetic Mutants and the UPC2, ECM22, and HAP1 Transcription Factors. Yeast Genetics and Molecular Biology Meeting. University of Wisconsin, Madison, July, 2002.

Valachovic, M., Wilcox, L., Balderes, D., Sturley, S., and Bard, M. Interaction of UPC2 and ECM22 Transcription Factors with Ergosterol Biosynthesis. International Conference on the Bioscience of Lipids (ICBL) Graz, Austria, Sept. 2002. Mo C., Valachovic M., Randall S.K., Nickels J.T. and Bard M. The Role of the ERG28 Gene Product in C-4 Demethylation Reactions in Yeast Sterol Biosynthesis. Johnson & Johnson Focused Giving Symposium. New Brunswick, NJ, Dec. 2002. Mo C., Randall S., and Bard M. Can We Identify Proteins in the Ergosterol Enzymatic Complex? The INGEN Proteomics Symposium. Indianapolis, IN, Nov. 2002. Pierson, C.A., Jia, N. Lees, N.D., Eckstein, J., Barbuch, R., and Bard, M. Isolation, Characterization, and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-Keto Reductase. Johnson & Johnson Focused Giving Symposium. New Brunswick, NJ, Dec. 2002. Valachovic, M., Wilcox, L., Balderes, D., Sturley, S., and Bard, M. Synthetic Lethality between Ergosterol Biosynthetic Mutants and the UPC2, ECM22, and HAP1 Transcription Factors. Johnson & Johnson Focused Giving Symposium. New Brunswick, NJ, Dec. 2002. Elmore, B. and Bard, M. Transcriptional Regulation of the Essential Sterol Genes ERG25 and ERG27 in Saccharomyces cerevisiae. Indiana Branch of the American Society for Microbiology. Spring Mill State Park, Mitchell, IN, April, 2003 Rogers, K. and Bard, M. Disruption and Characterization of Cytochrome b5 in Candida albicans. Indiana Branch of the American Society for Microbiology. Spring Mill State Park, Mitchell, IN, April, 2003. Pierson, C.A., Jia, N., Mo, C., Lees, N.D., Sturm, A.S., Eckstein, J., Barbuch, R., and Bard, M. Isolation, characterization, and Regualtion of the Candida albicans ERG27 Gene Encoding the Sterol 3-Keto Reductase. The 15th Congress of the International Society for Human and Animal Mycology, San Antonio, TX, May 2003, p.410. Mo, C., Milla, P., Athenstaedt, K., Ott, R., Balliano, G., Daum, G. and Bard, M. The 3Keto Reductase Protein (Erg27p) is Required for Oxidosqualene Cyclase (Erg27p) in Yeast. 6th Yeast Lipid conference, Colmar , France, May 2003. Rahier, A., Darnet, S., Taton, M., Husselstein, T., Benveniste, P., and Bard, M. Cloning and Characterization by Expression in Yeast on Non-Heme iron from Oxygenases Involved in Plant Sterol Biosynthesis. 6th Yeast Lipid Conference, Colmar, France, May 2003.

Darnet, S., Bard, M., and Rahier, A. Cloning and Functional characterization of Sterol4-Methyl Oxidase cDNAs from Arabidopsis thaliana by Complementation of a Yeast erg25 Mutant Lacking Sterol-4-Methyl Oxidation. 6th Yeast Lipid Conference, Colmar , France, May 2003. Mo, C., Milla, P., Athenstaedt, K., Ott, R., Balliano, G., Daum, G. and Bard, M. In Yeast Sterol Biosynthesis the 3-Keto Reductase Protein (Erg27p) is Required for Oxidosqualene Cyclase (Erg27p). Johnson & Johnson Focused Giving Symposium. New Brunswick, NJ, Dec. 2003. Pierson, C.A., Jia, N., Mo, C., Lees, N.D., Sturm, A.S., Eckstein, J., Barbuch, R., and Bard, M. Isolation, characterization, and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-Keto Reductase. Johnson & Johnson Focused Giving Symposium. New Brunswick, NJ, Dec. 2003. Tsai, H., Bard, M., Izumikawa, K., Krol, A.A., Sturm, A.M., Culbertson, N.T., Pierson, C.A., and Bennett, J. A Candida Glabrata ERG1 Mutant with Increased Sensitivity to Azoles and to Low Oxygen Tension. ASM Conference, Candida and Candidiasis (7th), Austin, TX, March, 2004, p. 114 Valachovic, M., and Bard, M. Suppression of the Synthetic Lethality in the Yeast Ergosterol Pathway. American Oil Chemists Society.Annual Meeting & Expo. Cincinnati, OH, May 2004, p. 18 Crisp, R., Valachovic, M., Bard, M., Winge, D., and Kaplan, J. Transcription of the Yeast Iron Regulon Responds Not Directly to Iron but to Iron-sulfur Cluster Biosynthesis. Yeast Genetics and Molecular Biology Mtg. Seattle, WA. July, 2004,p.172. Wright, R., Felthauser, A., Merkel, J., Sturm, A., and Bard, M. Cold Adaption in Yeast: the Role of ER-associated Degradation and Sterol Biosynthesis. American Society for Cell Biology. 45th Annual Meeting, San Francisco, CA, December, 2005 # 598 Bhuiyan, S., Bareither, B., Valachovic, M., and Bard, M. Suppressors of Synthetic Lethality Due to Cumulative Mutations Affecting Sterol biosynthesis in the Yeast Saccharomyces cerevisiae. American Oil Chemists Society.Annual Meeting & Expo.St. Louis, MO, May 2006, p. 21 Teske, B., Taramino, S., Barbuch, R., Eckstein, J., Balliano, G., and Bard, M. The Interaction of 3-Keto Reductase and Oxidosqualene Cyclase, Two Key Enzymes in the Ergosterol Biosynthetic Pathway. Indiana University SOM, Biochemistry and Molecular Biology Department Retreat Poster Session, September 22, 2006 Bhuiyan, M. S. A., Valachovic, M., Bareither, B. M., Eckstein, J. , Barbuch, R., Balderes, D., Wilcox, L., Sturley, S. L., Dickson, R. C. and Bard, M.Synthetic

Lethal and Suppressor Interactions between Sterol and Sphingolipid Pathways in Saccharomyces cerevisiae. Indiana University SOM, Biochemistry and Molecular Biology Department Retreat Poster Session, September 22, 2006 Valachovic, M., Bareither, B.M., Bhuiyan, M. S. A., Eckstein, J. , Barbuch, R., Balderes, D., Wilcox, L., Sturley, S. L., Dickson, R. C. and Bard. Alterations in Sphingolipid Biogenesis Suppress Defects in Yeast Ergosterol Metabolism . FEBS Special Meeting - European Lipidomics Initiative, Netherlands, October 21-25, 2006 Takemori, D., Bard, M., Tanabe, K., Niimi, M., Wu, S., Hodgson, W., Aoyama, T., Chibana, H., and Nakayama, H. Candida glabrata Sterol Transporter Gene CgAUS1 Confers Resistance to Antifungal Drugs. Molecular Biology Society of Japan 2006 Forum, #2P-310. Nagoya, Japan, December 6-8, 2006. Teske, B., Taramino, S., Barbuch, R., Eckstein, J., Balliano, G., and Bard, M. The Interaction of 3-Keto Reductase and Oxidosqualene Cyclase, Two Key Enzymes in the Ergosterol Biosynthetic Pathway. Johnson & Johnson Focused Funding Symposium. New Brunswick, NJ, November 28, 2006. Oliaro-Bosso, S., Teske, B., Taramino, S., Viola, F., Bard, M., and Balliano, G. Protein-Protein Interaction in Sterol Biosynthesis: the Relationship Between Sterol 3-ketoreductase and Oxidosqualene Cyclase, Annual Congress of the Italian Biochemical Society (SIB), Riccione, Italy September, 2006 Teske, B., Taramino, S., Bhuiyan, M.S.A., Mo, C., Balliano, G., and Bard, M. Protein-Protein Interactions Among Ergosterol Biosynthesis Enzymes Erg7p/Erg27p and Two-Hybrid Interactions Involving Are1p and Are2p. 8th Yeast Lipid Conference, Torino, Italy, May, 2007 Teske, B., Taramino, S., Bhuiyan, M.S.A., Mo, C., Balliano, G., and Bard, M. Mutational analysis of the Yeast 3-Ketoreductase (Erg7p) and the Effects on Ergosterol Biosynthesis. 8th Yeast Lipid Conference, Torino, Italy, May, 2007 Felthauser, A Ballou, J. Wright, R. and Bard, M. Cold Adaptation and Growth of Yeast at Low Temperatures Involves Diverse Cellular Functions and Physiological Processes. 47th Annual Meeting for American Society for Cell Biology, Washington, D.C., December, 2007 Nakayama, H., Bard, M., Tanabe, K. Aoyama, T., Takemori, D., Hodgson, W., Wu, S., Kumaraswami, N. Metzler, L., Takano, Y., Chibana, H., and Niimi, M. The Candida glabrata Sterol Transporter CgAUS1 Protects Against Azole Toxicity in The Presence Of Serum. 7th Awaji International Forum on Infection and Immunity, Awaji, Japan, September, 2007

Fortier, Z., Grengs, L., von Hohenberg, M., Kanneganti, M., Kwong, K., Lin, S., Lou, X., Myhre, E., Petersen, K., Sarapas, J., Talledge, N., Zimmerman, K., Felthasuer, A., Bard, M., and Wright, R. Life in the Cold: Characterization of Ten Psychrophilic Yeaast Species. 48th Annual Meeting for American Society for Cell Biology, San Francisco, CA, December, 2008 Tang, F., Chandler, P., Sturm, A.M., and Bard, M. Intracellular Sterol Transport Controls Vacuole Fusion and Fission During Cellular Aging. 48th Annual Meeting for American Society for Cell Biology, San Francisco, CA, December, 2008 McLarren, K. , Severson, T., du Souich, C., Chou, A., Hendson, G., Cunningham, D., Herman, G., Bard, M., Kratz, L., Kelley, R.I., Marra, M., and Boerkoel, C.F. Elucidation of the biochemical basis of a newly described X-linked mental retardation syndrome. 2nd Ann. Can. Human Gen. Conf. Harrison Hot Springs, BC, Canada, May, 2009 McLarren, K., Severson, T., du Souich, C., Chou, A., Hendson, G., Cunningham, D., Herman, G., Bard, M., Kratz, L., Kelley, R. I., Marra, M., and Boerkoel, C. F. The frustration of disability or a biochemical predisposition: the case of NSDHL dosage. Soc. Biol. Psych., 64th Annual Scientific Convention, Vancouver, B.C. May, 2009. Taramino, S., Valachovic, M., Oliaro-Bosso, S., Viola, F., Teske, B., Bard, M., and Balliano, G. Interaction of oxidosqualene cyclase with C-4 sterol demthylase complex. 9thYeast Lipid Conference, Berlin, Germany, May, 2009 Ho, C.-H., Mangtanong, L., Barker, S., Baryshnikova, A., Costanzo, M., Johnson, C., Giaver, G., Nislow, C., Bard, M., Yoshida, M., and Boone, C. MOA Bioactive compounds using budding yeast Saccharomyces cerevisiae.The 10th International Conference on Systems Biology (ICSB), Stanford, CA, August, 2009 Whybrew, J. and Bard, M. Aerobic uptake of cholesterol by ergosterol auxotrophs in Candida glabrata. 2010 IUPUI Research Day, Indianapolis, April 9, 2010 Whybrew, J. M. and Bard, M. Random and site directed mutagenesis in Saccharomyces cerevisiae Annual Meeting of the Indiana Branch of the American Society for Microbiology. University of Southern Indiana, April 16-17, 2010 Taramino, S., Teske, B., Oliaro-Bosso, S., Viola, F., Bard, M. and Balliano, G. Different interaction between oxidosqualene cyclase and steroid-3-keto reductase in yeast and mammals. International Symposium on Microbial Lipids, from genomcs to Lipidomics, Vienna, Austria, May 13-15, 2010.

Nakayama, H., Tanabe K., Nagi, M., Chibana, H. and Bard, M. Characterization of genes that regulate sterol uptake in Candida glabrata. The 10th Awaji International Forum on Infection and Immunity, Awaji, Japan, Sept. 7-10, 2010 Chair M.S. Committee/Thesis Titles James R. Downing, Biochemical and Genetic Aspects of Sterol Regulation Involving 3Hydroxyl-3-Methylglutaryl coenzyme A Reductase, August 1980 Debbie Copple, Cloning and Disruption of the Yeast Sterol Methyl Transferase Gene, May 1988 M. Christine Broughton, Isolation and Characterization of Polyene Resistant Strains of Candida albicans, December, 1989 Will Ashman, Cloning and Disruption of the Yeast C-8 Sterol Isomerase Gene, May 1991 Linda Bennett, Restriction Mapping and Disruption of the Yeast C-5 Sterol Desaturase Gene, December 1992 Kelly Schweitzer, The Characterization of CDC41 and the Isolation of a cdc34 Suppressor Gene in Saccharomyces cerevisiae, August 1994 Theresa Johnson, Cloning, Sequencing and Disruption of the Candida albicans C-4 Sterol Methyl Oxidase Gene, December 1998 Kristin Pergakes, Disruption and Characterization of the Candida albicans C-24 Sterol Transmethylase Gene and Regulatory Analysis of Two Genes Involved in Sterol Esterification in Saccharomyces cerevisiae, May 1999 Kora E. Aaron, Cloning and Disruption of the Candida albicans C-3 sterol Dehydrogenase (C-4 Decarboxylase) Gene and a Search for Synthetic Lethality with hes1 in Saccharomyces cerevisiae, August 2000 Nan Jia, Cloning and Characterization of the ERG27 and ERG24 Genes in the Yeast Candida albicans, August 2001 Kristin M. Rogers, disruption and Characterization of the HEM1 and cytochrome b5 Genes in Candida albicans, August 2003 Charles A. Pierson, Characterization of the Saccharomyces cerevisiae ERG25 Gene Encoding the Sterol 4-methyloxidase, May 2004. Bradley K. Elmore*

Bart M. Bareither, Suppressors of Synthetic Lethality Due to Mutations Affecting the Ergosterol Biosynthetic Pathway in Saccharomyces cerevisiae, Dec. 2005 Brian Teske, The Interaction of the 3-ketoreductase (Erg27p) and the Oxidosqualene Cyclase (Erg7p) in Saccharomyces cerevisiae, August, 2007 Sung tse Wu, Sterol Uptake in Candida and Saccharomyces Species and Protein-Protein Interactions in the Ergosterol Biosynthetic Pathway, December, 2007 *has not received M.S. Degree Masters Thesis Committee Member Scott Tate- Biology, 1995 Elizabeth Smith Biology, 1995 Marla Scholfield-Biology, 1998 Bolan Linghu-Biochemistry and Molecular Biology, 2001 Krista Barber, 2007 Chair Ph.D. Committees (Mentor) Beth A. Skaggs, Analysis of Three Ergosterol Biosynthetic Genes Involved in the Terminal Portion of the pathway in Saccharomyces cerevisiae, May 1996 Matthew A. Kennedy, Regulatory Analysis of Squalene Synthase (ERG9) from Saccharomyces cerevisiae and the Cloning and Characterization of the C-4 Sterol Methyloxidase Gene (ERG25) from Candida albicans, August 2000 Ph.D. Committees asMember (Date of Graduation): Christine OHara-Biology, 1992 Yun Liu- Biochemistry and Molecular Biology, 1994 Kyle Sloop- Biology, 2001 Kelly Schweitzer- Biochemistry and Molecular Biology, 2000 Ying Jiang- Biochemistry and Molecular Biology, 2001 Ke Zhan - Biochemistry and Molecular Biology, 2003 Kirk Kastasch Biochemistry and Molecular Biology Dean Wiseman Biology, 2004 Ross Cocklin-Biochemistry and Molecular Biology, 2009 Angela Deem-Biology David Huizinga- Biology, 2009 Brian Teske -Biochemistry and Molecular Biology Post-doctoral Fellows: Dr. Daniel Gachotte, August1995- April1999 Dr. Caiqing Mo, July 2000-December 2005

Dr. Vijaya Pagadala, June 2000-April 2001 Dr. Martin Valachovic, June 2001-May 2004 Dr. Md. Shah Alam Bhuiyan ,September 2004-present Dr. Selvamuthu N Kumaraswami, February 2006-present
Professional Activities:

Reviewed manuscripts for: Proc. Natl. Acad. Sci. (USA), Genetics, Antimicrobial Agents Chemother., J. Lipid Research, Eukaryotic Cell, Lipids, Molecular Microbiology, Applied and Environmental Microbiology, FEBS Lett., Drug Discovery Today, Experimental Microbiology, Topics in Current Genetics, Mol. Cell. Biol., EMBO J., and many others. Reviewed grant proposals for: NSF, USDA, FWF (Austrian Science Fund), Department of Veteran Affairs Invited Seminars/Presentations: Spotlight Lecturer, Purdue School of Science, Indianapolis, IN 1994 Johnson & Johnson, Focused Giving Program, New Brunswick, NJ 1995 American Oil Chemists Society Mtg. Sterol Symposium Indianapolis IN 1996 IUPU-Fort Wayne, 1997 Yeast Lipid Meeting, Keynote Speaker, Slomenice , Slovakia, 1998 University of Innsbruck, Institut fuer Biochemische Pharmakologie Innsbruck, Austria,1998 Rosetta Inpharmatics, Seattle, WA, 1998 Yeast Lipid Conference, Svendborg, Funen, Denmark, 2001 Yeast Lipid Conference, Turin Italy, May 2007 NSF, Division of Molecular& Cellular Biosciences, Arlington, VA, Febuary, 2008 (Protein-protein interactions in the yeast sterol biosynthetic pathway) Current Teaching Assignments Biology K322- Genetics and Molecular Biology undergraduate course for Biology majors; junior and senior level class of 125 students Biology K323- Laboratory in Genetics (supervisory role Biology 697-Microbial Genetics; graduate level, class of 25 graduate students Biology N322-Introductory Principles of Genetics, non-majors course Student Mentoring Citations Level 1-Remarkable and positive impact
Level 2Remarkable and positive impact; one of top 3

Level 3 Influenced the whole course of my life and effect on me is invaluable Year Level 1 Level 2 Level 3

1994-2009

124

77

22

Other Mentoring Maurice Broaddus Summer Research Opportunity Program, Howard Hughes Medical Institute Undergraduate Initiative in Biology-1990 Jannah Bacchus-LSAMP (Louis Stokes Alliance for Minority Participation), NSF-2008 Corey Johnson- Project SEED (Summer Enhancement for Economically Disadvantaged), American Chemical Society-2009 Service Department Director of Graduate Studies, 2010Forensics Search Committee, 2008 Promotion, Tenure, and Reappointment Committee-1977-present Promotion and Tenure Primary Committee, 1992-present Ph.D. Qualifying Exam: Molecular and Cell Biology, 1994-present Ph.D. Qualifying Exam: Membrane Biology (Chair), 1994-present Graduate Committee, 1981-present Graduate Recruiting Committee, 2001 TERA Panel, 2000 Curriculum Committee, 1980-1982 Implemented Biology Department Seminar Program, 1976-1977 Secretary Biology Faculty, 1975-1976 Multiple Search and Screen Committees School of Science Promotion and Tenure Unit Representative 1992-2009, Chair 2009-2010 Nominations and Awards 2001-6 Deans Administrative Advisory Committee, 1985-1989 Academic Appeals, 1990-2 Technology Transfer Committee, 1992-3 Joint Seminar Committee, SOS and Medicine, 1992-4 Committee on Committees, 1978-1982 Faculty Affairs, 1981-1982 Departmental Search and Screens Botanist, 1977, 1978 Geneticist 1980 Biology Chairman, 1985 (Chair) Ecologist, 1980 Faculty Affairs, 1981-982 Research Committee, University IUPUI Faculty Council, At Large Member, 2008 School of Science Steering Committee, 2008

University Sabbatical Committee, 2008 IUPUI Fringe Benefits Committee 2001-present NIH Roadmap Evaluation Committee, 2005 IUPUI Promotion and Tenure, 1996 Panel E, 1994, 1995 Institutional Biosafety Committee, 1988-1994 Future Studies Committee Faculty Review Board, 1980-1981 Committee for Science and Technology 1986-1987 Biotechnology Committee (Chair) 1983-4, 1992-6 Community Rohrer Meadows Home Owners Association Board Member, 2008-10 Chair, Microbiology and Molecular Biology Section, Indiana Academy of Sciences, 1990

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 CURRICULUM VITAE

VI. General Information

Name: Belecky-Adams, Teri Louise

Education and Training

B.S./1985 Ph.D./1994

University of Wyoming, Department of Psychology, Laramie, Wyoming University of Cincinnati College of Medicine, Department of Cell Biology, Neurobiology, and Anatomy, Cincinnati, Ohio

Postdoctoral Training: 4/94 5/96 Postdoctoral Fellow, Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD 5/96 7/01 Research Associate, Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD

Academic Appointments
7/01 to present 5/1996 to 7/2001 5/1994 to 5/1996 8/1989 to 4/1994 7/1985 to 8/1989

Assistant Professor, Department of Biology, Indiana University-Purdue University Research Associate, Department of Ophthalmology Wilmer Eye Institute, Johns Hopkins School of Medicine Postdoctoral Fellow, Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins School of Medicine Graduate Research Assistant, Department of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati College of Medicine Research Assistant II, Department of Otolaryngology and Maxillofacial Surgery, University of Cincinnati College of Medicine

Other Appointments:

Center for Regenerative Biology and Medicine, Biology Department, IUPUI Stark Neuroscience Institute, Indiana University School of Medicine

Professional Organizations:
2003 to Present 1995 to Present 1996 to Present 1995 to 2001 1991 to Present 1986 to 1991 1991-1993 1995 1997-1998 1997 -1998 1995-1998 2002

International Society for Eye Research Association for Research in Vision and Ophthalmology Society for Neurosciences Society for Developmental Biology American Society for Cell Biology Association for Chemoreception Sciences (AChemS) Albert J. Ryan Fellowship Award Retina Research Foundation/Lawrence Travel Fellowship Grant Fight for Sight Grant-in-Aid Knights of Templar Eye Foundation Award National Research Fellowship Award (F2EYO6642A) Purdue Summer Research Award

Honors and Awards:

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 2003 2003 Indiana University nominee for the Searles Scholar Award March of Dimes, Basil OConnor Starter Scholarship Award

Teaching Assignments:
Class 571 697 571 697 571 697 571 697 571 697 D556 571 697 571 697 556 571 697 Semester Spring 2002 Fall 2002 Spring 2003 Fall 2003 Spring 2004 Fall 2004 Spring 2005 Fall 2005 Spring 2006 Fall 2006 Spring 2006 Spring 2007 Fall 2007 Spring 2008 Fall 2008 Fall 2008 Spring 2009 Fall 2009 Title Developmental Neurobiology Sensory Systems Developmental Neurobiology Sensory Systems Developmental Neurobiology Sensory Systems Developmental Neurobiology Sensory Systems Developmental Neurobiology Sensory Systems Biotechniques Developmental Neurobiology Sensory Systems Developmental Neurobiology Sensory Systems Physiology I Developmental Neurobiology Sensory System Enrollment 12 15 28 19 26 18 30 38 31 45 6 67 49 65 58 80 77 63 Contact hours 3/wk 3/wk 3/wk 3/wk 3/wk 3/wk 3/wk 3/wk 3/wk 3/wk 6/sem 3/wk 3/wk 3/wk 3/wk 9/sem 3/wk 3/wk

Service to Students:

Primary Professor to the Following Students: Rachna Sehgal a,b , PhD student Jonathan Wilson a.b , MS student
a b

Indicates students who are authors on peer-reviewed publications from my lab Indicates students whose results were presented at scientific conferences Chair, Julie Whitsett Co-chair, Rachna Sehgal Co-chair, Sophie Liu Chair, Jonathan Wilson Jesse Savage Dominique Kopatz Ben Yaden Brooke West Jennifer Funke Michael Anne Shane Mona Lisa Muchatuta Charity Nofziger Stephanie Colvin Justin Van Horn Julie Hayden Aimee Banga Kaarthik Chandrasekhar Behnaz Saranjami Nicci Billingsley

Dissertation Committees

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10

Undergraduate Research and Theses (K493-494)

Nickolaus Frechette Brandon Anderson*# Jada Hake* Carolyn Hayes* Chad Gavigan Shalini Jani* Miranda Shook*# Victoria Vu Miranda Murray*

Dirk Andres* Aiman Haqqani* Katelyn Fabeyan* Marianne Steffan* Andrea Abdullah^ Nida Habeeb* Heather Stout Jason Moor* Sam Townsley*^

* Indicates students who have finished their undergraduate thesis in my lab. # Indicates student who received Undergraduate Research Opportunity (UROP) Funds to present their work at international meetings. ^ Indicates minority students who received research awards to work in my laboratory.

Participation in Minority Research Programs:

McNair Minority Summer Fellowship Mentor, Karima Fitzgerald, 2003 Diversity Scholar Mentor, Andrea Abdullah, 2006-2008 Louis Stokes Alliance for Minority Participation, Samantha Townsley, 2007 T35 Summer Minority Research Summer Research Blog, Samantha Townsley

Professional Service
Manuscript Reviews While in Rank Experimental Eye Research-1 Development 6 Developmental Biology-2 Mechanisms of Development -4 Molecular Vision- 3 Journal of Neuroscience -4 Investigative Ophthalmology and Visual Sciences-5 Developmental Dynamics 2 BioMed Central Developmental Biology-3 Stem Cells - 1

University Service
School: 2006-07 2006-07 2006-08 2009-2010 School of Science International Travel Awards Committee Neural and Endocrine Group Representative for the Center of Regenerative Biology and Medicine School of Science Representative , IUPUI Faculty Council University Faculty Council for Indiana University

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 Departmental: 2001-02 Graduate Recruiting Committee 2002-03 Chair, Search and Screen for new Asst. Professor of Biology 2002-08 Graduate Recruiting Committee 2002-03 Chair, Graduate Recruiting Committee 2006-present Department of Biology Seminar Committee 2009 Search and Screen for new Academic Specialist

Other Professional Activities: Invited Seminars (while in rank)

11/08 University of Houston Department of Optometry: Bone Morphogenetic Proteins in Optic Nerve and Optic Cup Astrocytes 10/07 Department of Biology, Marian College, Indianapolis, IN Regeneration of the Retina 10/06 Department of Biology, Marian College, Indianapolis, IN Dorso-Ventral Patterning of the Chick Eye. 11/05 University of Cincinnati Childrens Hospital Dept. of Ophthalmology, Cincinnati, OH Which end is up? The TGF-b Family in Retinal Patterning. 12/05 Department of Biology, Marian College, Indianapolis, IN Dorso-Ventral Patterning of the Chick Eye. 11/04 Department of Biology, Marian College, Indianapolis, IN Bone Morphogenetic Proteins and Retinal Patterning in the Chick 2/04 2/04 4/03 4/03 8/02 4/02 Department of Cell Biology, I.U. School of Medicine, Indianapolis, IN Bone Morphogenetic Proteins and Retinal Patterning in the Chick. Department of Biology, IUPUI, Indianapolis, IN Dorso-Ventral Patterning of the Chick Eye and Pax2 Regulation Department of Basic Medical Sciences, Purdue University, West Lafayette, IN Dorso-Ventral Patterning of the Chick eye. Department of Zoology, Miami University, Oxford, OH Dorso-Ventral Patterning of the Chick Eye. Washington University School of Medicine, St Louis, MO. Bone Morphogenetic Proteins and Retinal Patterning in the Chick. School of Medicine, Indiana University, Bloomington, IN Bone Morphogenetic Proteins and Retinal Patterning in the Chick.

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 9/01 Department of Biochemistry, I.U. School of Medicine, Indianapolis, IN Bone Morphogenetic Proteins and Retinal Patterning in the Chick

Platform Talks at Regional, National, International Meetings While in Rank 5/09 Association for Research in Vision and Ophthalmology, Ft. Lauderdale, FL. 5/07 Experimental Biology 2007, Expression of RNA Binding Protein, MSI, I Developing Chick Retina . National Meeting of the American Societies of Experimental Biology, Washington D.C.

11/06 Sehgal R and Belecky Adams, T. (2006) Effect of Bone Morphogenetic Protein 7 (BMP7), Sonic Hedgehog (Shh) and Fibroblast Growth Factor 8 (FGF8) on Pax2 Expression. Great Lakes Vision Research Conference, Ann Arbor, MI. 4/04 BeleckyAdams TL, Carlson S, and Shook M (2004) Pax2 and Patterning in the Chick Optic Cup. International Congress on Eye Research, Experimental Eye Research, Sidneye, Australia

Regional, National, International Poster Presentations While in Rank Wilson JM, Sato K, Chernoff E, Belecky-Adams TL. (2006) Expression of RNA Binding Protein, MSI-1, in Developing Chick Retina. Great Lakes Vision Research Conference, Ann Arbor, MI. Belecky-Adams TL, Andres DJ, Adler R, Sehgal R. (2006) Bone Morphogenetic Protein 7 Increases Chick Photoreceptor Outer Segment Initiation. Great Lakes Vision Research Conference, Ann Arbor, MI. Qi L, Belecky-Adams TL, and Rhodes SJ. (2005) The Expression of Lhx2 and Lhx3 LIM Homeodomain Transcription Factors in Xenopus Retinal Development and Regeneration. Great Lakes Vision Research Conference, Cincinnati, OH. Wilson JM, Sato K, Chernoff E, Belecky-Adams, TL (2005) Expression of RNA Binding Protein, MSI-1 in Developing Chick Retina. Great Lakes Vision Research Conference, Cincinnati, OH. Sehgal R and Belecky-Adams TL (2005) Effect of Bone Morphogenetic Protein 7, Sonic Hedgehog, and Fibroblast Growth Factor on Pax2 Protein Expression. Great Lakes Vision Research Conference, Cincinnati, OH. Belecky-Adams TL, Karcavich R, and Hinjosa L. (2005) Retinal Regeneration After Axotomy in Xenopus laevis. Great Lakes Vision Research Conference, Cincinnati, OH. Belecky-Adams TL, Carlson S, Anderson B, and Whitsett J (2003) The Role of Bone Morphogenetic Proteins in Chick Eye Patterning and Axonal Pathfinding. Biocomplexity workshop, Bloomington, IN.

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10

Research Workshops Attended While in Rank

Grant Writing Workshop, Fall 2008 Vision, Aging, and the Brain, Association for research in Vision and Ophthalmology, Ft. Lauderdale, FL 5/2007 Informatics and Visual Science, Association for research in Vision and Ophthalmology, Ft. Lauderdale, FL 5/2006 Workshop on Biocomplexity in Regeneration, Bloomington, Indiana 5/2005 Proteomics, Saturday Workshop, Association for research in Vision and Ophthalmology, Ft. Lauderdale, FL 5/2002 Retinal Regeneration, Ft. Lauderdale, FL 5/2002

Grants Obtained While in Rank: Currently Active Grants Obtained in Rank

External Awards BMPs and Glaucoma Role: Principal Investigator Agency: American Health Assistance Foundation Amount: 100,000 Dates: 4/2008 to 3/2010 Goal: To determine the role of BMP7 in regulation of Pax2 expression in retinal astrocytes in vitro and ganglion cell survival in the retina. Astrocyte Development and Reactive Gliosis Role: Principal Investigator Agency: National Institutes of Health Amount: $1,243,778 Dates: 09-30-2009 to 9-29-2013 Goal: To investigate the hypothesis that the intracellular signaling pathways of the bone morphogenetic proteins and sonic hedgehog cooperate in the modulating the activity of repressor protein TLX at the Pax2 promoter in vitro and in vivo during development and reactive gliosis. Mechanisms of Astrocyte Development Role: Principal Investigator Agency: National Institutes of Health Amount: $376,425 Dates: 09/01/2010 to 08/31/2013 Goal: To investigate the role of BMP and SHH signaling pathways in the development of mouse optic nerve astrocytes.

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10

Previously Funded Grants While in Rank

Internal Awards Title: Stem Cells, Musashi and Amphibian Spinal Cord Regeneration Principal Investigator: Ellen Chernoff Role: Co-PI Sponsoring Agency: Morton Cure for Paralysis Foundation Funding level: $50,000 Dates: 4/2007 to 12/2009 Title: Optic Stalk Development, RSFG Dates: 7/01/06 to 7/08 Funding level: $30,000 Principal Investigator: Teri Belecky-Adams Role: PI Sponsoring agency: IU Institutional grant Title: Bone morphogenetic proteins in the differentiation of the ventral chick retina. Dates: July-August, 2002 Funding level: $6000 Principal Investigator: Teri Belecky-Adams Role: PI Sponsoring agency: Purdue University internal funds Title: The role of putative oncogene Pax2 in mitosis and potential regulation by bone morphogenetic proteins. Dates: 9/01/2002 to 8/31/20003 Funding level: $20,000 Principal Investigator: Teri Belecky-Adams Role: PI Sponsoring agency: American Cancer Society External Awards Title: Problems of Amphibian Limb and Spinal Cord Regeneration Dates: 10/01/06 9/30/09 Funding level: $1,600,000 Principal Investigator: David Stocum Role: Collaborator Sponsoring agency: W.M. Keck Foundation My Contribution: 1) amputation of stage 60 Xenopus limbs and preparation of limbs for proteomic analysis at specific times following amputation in collaboration with David Stocums lab, 2) histological and immunohistochemical analysis of surgically altered limbs in collaboration with David Stocums lab, 3) determination of the role of BMPs in limb regeneration in collaboration with Ellen Chernoffs lab. Goal: To understand the molecular mechanisms of amphibian limb and spinal cord regeneration. Title: The regulation and potential role of Pax2 in optic stalk development Dates: 2/01/2003 to 4/31/2004 Funding level: $180,000 Principal Investigator: Teri Belecky-Adams Role: PI Sponsoring agency: March of Dimes Title: Discovering the Secrets of Regeneration: a Comparative Strategy Dates: 7/01/04 to 6/30/07 Funding level $1,600,000 Principal Investigator: David Stocum and members of the Regeneration Ctr Co-PIs- Teri Belecky-Adams, Ellen Chernoff, Ros Smith, Tony Mescher, Tony Neff, Mike King Sponsoring agency: Indiana 21st Century Research and Technology Fund

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10

Print and Electronic Publications Evidence of Scholarly Peer-Reviewed Publications:

Peer-Reviewed Manuscripts Wilson, JM, Martinez-DeLuna, R, El Hodiri, HM, Smith, R, King, MW, Mescher, AL, Neff, AW, and Belecky-Adams, TL. RNA helicase Ddx39 is a marker of proliferating cells in the developing and regenerating retina of Xenopus laevis. Gene Expr Patterns. 2010 Jan;10(1):44-52. Sehgal, R, Sheibani, N, Rhodes, SJ, Belecky-Adams, TL. (2009) BMP7 and SHH regulate Pax2 in retinal astrocytes by relieving TLX repression. Dev. Biol. 332: 429-443. . Sehgal, R, Karcavich, R, Carlson, S, and Belecky-Adams, TL (2008) Ectopic PAX2 expression in the ventral optic cup phenocopies loss of PAX2 expression. Dev. Biol. 319:23-33. All of the contributing authors were members of the Belecky-Adams lab, therefore contributions of authors has not been detailed. Wilson JM, Sato K, Chernoff EA, Belecky-Adams TL. (2007) Expression patterns of chick Musashi-1 in the developing nervous system. Gene Expr Patterns: 7(7):817-25. Sehgal R, Andres DJ, Adler R, Belecky-Adams TL. (2006) Bone morphogenetic protein 7 increases chick photoreceptor outer segment initiation. Invest Ophthalmol Vis Sci. 47(8):362534. Fong SL, Criswell MH, Belecky-Adams T, Fong WB, McClintick JN, Kao WW, Edenberg HJ. (2005) Characterization of a transgenic mouse line lacking photoreceptor development within the ventral retina. Exp Eye Res. 81(4):376-88. Belecky-Adams T, Holmes M, Shan Y, Tedesco CS, Mascari C, Kaul A, Wight DC, Morris RE, Sussman M, Diamond J, Parysek LM. (2003) An intact intermediate filament network is required for collateral sprouting of small diameter nerve fibers. J Neurosci. 23(s28):9312-9. Belecky-Adams TL, Adler R, Beebe DC. (2002) Bone morphogenetic protein signaling and the initiation of lens fiber cell differentiation. Development 129(16):3795-802. Adler R, Belecky-Adams TL. (2002) The role of bone morphogenetic proteins in the differentiation of the ventral optic cup. Development 129(13):3161-71. Adler R, Tamres A, Bradford RL, Belecky-Adams TL. (2001) Microenvironmental regulation of visual pigment expression in the chick retina. Dev Biol. 236(2):454-64. Belecky-Adams T, Adler R. (2001) Developmental expression patterns of bone morphogenetic proteins, receptors, and binding proteins in the chick retina. J Comp Neurol. 430(4):562-72.

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 Belecky-Adams TL, Scheurer D, Adler R. (1999) Activin family members in the developing chick retina: expression patterns, protein distribution, and in vitro effects. Dev Biol. 210(1):10723. Weng J, Belecky-Adams T, Adler R, Travis GH. (1998) Identification of two rds/peripherin homologs in the chick retina. Invest Ophthalmol Vis Sci. 39(2):440-3. Belecky-Adams T, Tomarev S, Li HS, Ploder L, McInnes RR, Sundin O, Adler R. (1997) Pax-6, Prox 1, and Chx10 homeobox gene expression correlates with phenotypic fate of retinal precursor cells. Invest Ophthalmol Vis Sci. 38(7):1293-303. Belecky-Adams T, Cook B, Adler R. (1996) Correlations between terminal mitosis and differentiated fate of retinal precursor cells in vivo and in vitro: analysis with the "windowlabeling" technique. Dev Biol. 178(2):304-15. Belecky-Adams T, Wight DC, Kopchick JJ, Parysek LM. (1993) Intragenic sequences are required for cell type-specific and injury-induced expression of the rat peripherin gene. J Neurosci. 13(12):5056-65. Brining SK, Belecky TL, Smith DV. (1991) Taste reactivity in the hamster. Physiol Behav. 49(6):1265-72. Belecky TL, Smith DV. (1990) Postnatal development of palatal and laryngeal taste buds in the hamster. J Comp Neurol. 293(4):646-54. Book Chapters Belecky-Adams TL, Wilson J, del Rio-Tsonis K. (2008) Building and re-building the chick retina. Animal Models for Eye Research. Eds. Panagiotis A. Tsonis and Jocken Wittbrodt, Elsevier, New York, pp 102-119. Adler R and Belecky-Adams T (1999) Cell fate and determination in the chick embryo retina. Cell Fate and Lineage Determination. Ed. Sally Moody, Academic Press, San Diego, pp463-474. Published Meeting Abstracts While in Rank Bone Morphogenetic Protein 7 (BMP7) and Sonic Hedgehog (SHH) Regulate Pax2 in Retinal Astrocytes by Relieving Tlx Repression. Invest. Ophthalmol. Vis. Sci.. 48: E-Abstract 2528. Belecky-Adams TL, Wilson J, Sato K, and Chernoff EA. (2007) Musashi Expression in Developing and Mature Chick Eye. FASEB J. 21:324.6 Sehgal R, and Belecky Adams T. (2007) Effect of Bone Morphogenetic Protein 7 (BMP7), Sonic Hedgehog (Shh) and Fibroblast Growth Factor 8 (FGF8) on Pax2 Expression. Invest. Ophthalmol. Vis. Sci.. 48: E-Abstract 3116. Belecky-Adams TL and Qi L. (2007) Retina Axotomy in Xenopus Laevis Invest. Ophthalmol. Vis. Sci. 48: E-Abstract 53.

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 Wilson JM, King AM, Mescher AL, Neff AW, and Belecky-Adams TL. (2007) Ddx39 in Developing and Regenerating Xenopus Retina. Invest. Ophthalmol. Vis. Sci. 2048: E-Abstract 5703. Wilson JM, Sato K, Chernoff E, and BeleckyAdams TL (2006) Expression of RNA Binding Protein, MSI, in Developing Chick Retina. Invest. Ophthalmol. Vis. Sci. 47: E-Abstract 5745. Sehgal R, Andres D, Jay JJ, Carlson S, Adler R, and BeleckyAdams T. (2005) BMP7 Effects on Chick Retinal Cells in vitro. Invest. Ophthalmol. Vis. Sci. 46: E-Abstract 3972. Karcavich RE, Scott C, Fong S-L, Fong W-B, and BeleckyAdams T (2005) Rod Development in a Mutant Mouse With Diminished Cones. Invest. Ophthalmol. Vis. Sci. 46: E-Abstract 3977. BeleckyAdams TL, Shook M, and Scott C. (2004) Ectopic expression of paired homeobox transcription factor PAX2 in the developing chick optic cup. Invest. Ophthalmol. Vis. Sci. 45: EAbstract 700. Belecky-Adams TL, Anderson B, and Adler R. (2003) Bone Morphogenetic Protein 7 (BMP7) Stimulates Outer Segment Elongation of Chick Photoreceptors in vitro. Invest. Ophthalmol. Vis. Sci. 44: E-Abstract 2813. Belecky-Adams TL and Adler R. (2002) Bone Morphogenetic Proteins (BMPs) in the Differentiation of the Ventral Optic Cup. Invest. Ophthalmol. Vis. Sci. 2002 43: E-Abstract 825. Beebe DC, Belecky-Adams TL, and Adler R. (2002) Signaling Through BMP and Activin Receptors Regulates Lens Fiber Cell Differentiation. Invest. Ophthalmol. Vis. Sci. 2002 43: EAbstract 3882. Abstracts Published Prior to Arrival at IUPUI Belecky-Adams TL, Bradford RB, and Adler R. (2002) Separate expression domains of bone morphogenetic proteins and their receptors in the developing chick eye. Invest. Ophthalmol. Vis. Sci.43. Beebe DC, Belecky-Adams TL, and Adler R. (2002) Signaling through BMP and activin receptors regulates lens fiber cell differentiation. Invest. Ophthalmol. Vis. Sci. e-abstract 3882. Belecky-Adams TL and Adler R. (1999) Developmental expression patterns of bone morphogenetic protein family members and receptors in the chick eye. Invest. Ophthalmol. Vis. Sci.40: S2050. Tamres AN, Belecky-Adams TL, and Adler R. (1999) Microenvironmental regulation of visual pigment expression in the chick retina. Invest. Ophthalmol. Vis. Sci. 40: S2042. Belecky-Adams TL, Scheurer D, and Adler R. (1998) Activin family members in the developing chick retina: expression patterns, protein distribution, and in vitro effects. Gordon Research Conferences on Neural Development.

10

Teri L. Belecky-Adams, Ph.D. Revised 12/01/10 Belecky-Adams TL, and Adler R. (1998) Developmetal expression patterns of activin family members in the chick eye. Invest. Ophthalmol. Vis. Sci. 39: S217. Adler R, Scheurer D, Belecky-Adams T. (1998) Effects of activin on embryonic retinal neurons and photoreceptors. Invest.Ophthalmol. Vis. Sci. 39: S217. Belecky-Adams TL and Adler R. (1997) Stage-dependent restrictions in the developmental potential of chick retinal precursor cells. Mid-Atlantic Regional Developmental Biology Conference, Washington, DC. Belecky-Adams TL, and Adler R. (1996) Stage-dependent restrictions in the developmental potential of retinal precursor cells. Society for Neurosceinces. Belecky-Adams T, Tomarev S, Sundin O, McInnes RR, Ploder L, and Adler R. (1996) Invest. Ophthalmol. Vis. Sci. 37: S1050. Weng J, Belecky-Adams TL, Adler R, and Travis GH. (1996) The chicke homologue of RDS/peripherin is expressed in photoreceptor outer segments. Invest. Ophthalmol. Vis. Sci. 37: S805. Saga T, Scheurer D, Belecky-Adams T, and Adler R. (1996) Outer Segment morphogenesis in cultures of undifferentiated photoreceptor precursor cells. Invest. Ophthalmol. Vis. Sci. 37: S624. Belecky-Adams TL., Cook BE and Adler RA. (1995) Plasticity of retinal precursor cells after terminal mitosis: in vivo and in vitro studies. Invest. Ophthalmol. Vis. Sci. 36: S648. Adler R, Belecky-Adams TL, Tomarev S, and Sundin O. (1995) Correlation between Prox1 and Pax6 homeobox gene expression and phenotypic fates of retinal precursor cells. Invest. Ophthalmol. Vis. Sci. 35 of transgenic mice alters neuronal inte: S28. Belecky-Adams T, Wight DC, Kopchick JJ, and Parysek LM. (1993) Disruption of peripherin network in dorsal root ganglion neurons rmediate filament expression. Soc Neurosci. 19 Belecky-Adams TL, Di Carlo T, Wight DC, Ley CA, Kopchick JJ, and Parysek LM. (1991) 5.8Kb of 5 flaking sequence of the peripherin gene is insufficient to direct cell-type specific expression of a tagged peripherin gene in transgenic animals. J. Cell Biol. 155, 295a. Smith DV, Akeson RA, Shipley MT, Belecky TL. (1989) Inervation-dependent expression of neural-related antigens in rat taste cells. Soc Neurosci 15 (1) 752. Smith DV, Belecky TL, and Hanamori T. (1989) Laryngeal taste buds in the hamster: morphological development and chemical sensitivity. Chem. Senses 14. Belecky TL and Smith DV (1988) Early postnatal developmet of laryngeal and palatal taste buds in the hamster. Chem. Senses 13 (4): 675. Brining SK, Belecky TL, and Smith DV. (1988) Taste reactivity in the hamster. Chem Senses 13 (4):677.

11

Bonnie L. Blazer-Yost

Curriculum Vitae
BONNIE L. BLAZER-YOST, Ph.D. Present Address: Office Address: 50 Highland Manor Court Indianapolis, Indiana 46228 Department of Biology Indiana University/Purdue University at Indianapolis 723 West Michigan Street Indianapolis, IN 46202-5132 Contact: 317-278-1145 bblazer@iupui.edu

Education: 1969-73 1976-84

B.A. Lebanon Valley College, Annville, PA (Chemistry Major) Ph.D. University of Pennsylvania, Philadelphia, PA

Postgraduate Training and Fellowship Appointments: 1981-84 National Research Service Award - Cell and Molecular Biology Training Grant, University of Pennsylvania. 1984-86 Post-doctoral Fellowship, Department of Pharmacology, Cambridge University, Cambridge, U.K. Faculty Appointments: 2007-present Professor, Biology Department, School of Science, Indiana University/ Purdue University at Indianapolis (IUPUI), IN Adjunct Professor, Depart. of Integrative and Cellular Physiology, IUSM Adjunct Professor, Department of Anatomy and Cell Biology, IUSM 1998 - 2007 Associate Professor, Biology Department, School of Science, IUPUI Adjunct Associate Professor, Depart. of Integrative and Cellular Physiology, IUSM Adjunct Associate Professor, Department of Anatomy and Cell Biology, IUSM 1993-98 Assistant Professor, Biology Department, School of Science, IUPUI 1993 -98 Adjunct Assistant Professor, Department of Physiology and Biophysics, IUSM 1993-98 Adjunct Assistant Professor, Department of Anatomy, IUSM 1987-93 Research Assistant Professor, Departments of Medicine and Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 1986-87 Research Associate, University of Pennsylvania School of Medicine, Phila. PA Awards, Honors and Memberships in Honorary Societies: 1984-85 Ann Horton Visiting Fellow, Newnham College, Univ. Cambridge, Cambridge 1984-86 Individual National Research Service Award, N.I.H. 1995 Chairperson, Cell Biology Section, Indiana Academy of Science 1995-96 President, Midwest Biological Transport Group 1997 + 2000 Teaching Excellence Recognition Award 2002 Dunaway-Burnham Visiting Fellow, Physiology Department, Dartmouth University

Bonnie L. Blazer-Yost Committee Membership: 1994-1997 Research and Development Committee, Indianapolis V.A.M.C. 1995-present Animal Use Committee, Veterans Affairs Medical Center 1996-1998 Fee Appeals Committee, IUPUI 1996-1998 Academic Affairs Committee of the IUPUI Faculty Council 1996-1998 Steering Committee - School of Science 1996-1998 Steering Committee - Cell and Integrative Biology Training Program 1998-1999 Promotions and Tenure Committee - Department of Biology 1998-1999 Academic Affairs - University Committee 1998-2003 Faculty Affairs - University Committee 1999 Search Committee Associate Dean of the I.U. Graduate School 2002 - 2004 International Physiology Committee of the American Physiological Society 2004 2005 Medical Advisory Board, National Kidney Foundation of Indiana 2004 - 2007 IUPUI School of Science Research Committee 2005-present Promotion and Tenure Committee - Biology Department, IUPUI 2005-2009 Faculty Affairs University Committee 2005-2008 Fellowship subcommittee of the IUPUI University Graduate Affairs Committee 2006-2009 IUPUI Faculty Council At Large Representative 2008-2010 University Promotion and Tenure Committee

Other Recent University Service: 1998 Judge, 2nd annual School of Science Graduate Research Symposium 1998 TERA Award Committee 1998 Arranged an American Heart Association Research tour of IUPUI, talks by American Heart Grant awardees and Lunch for donors 2001 Lowe Syndrome Association, 600-mile Walk Celebration Laboratory tours IUPUI conference center and Biology department. 2003 Reviewer UROP proposals submitted to IUPUI 2005 Judge Sigma Xi Graduate Student Research Symposium 2005 Established and initiated an international exchange program between IUPUI and University Magna Graecia, Catanzaro, Italy 2005 Organized and convened the first ENaC joint symposium 10/8/05. Investigators from 6 departments in the School of Science, the School of Medicine and Purdue, West Lafayette attended and presented their research. Memberships in Professional Societies: 1984 British Society for Cell Biology 1987 Society of General Physiologists 1990 American Physiological Society 1994 Midwest Biological Transport Group (American Physiological Society) 2005 International Society of Nephrology 2007 American Society of Nephrology

Bonnie L. Blazer-Yost Membership on Editorial Boards 1999-present American Journal of Physiology 2003- 2007 Pflugers Archiv - European Journal of Physiology Organizer of National/International Meetings Co-organizer with Prof. Nicola Perrotti of an international meeting entitled Using Rodent Models to Study Kidney and Liver Disease. Magna Graecia University, Catanzaro, Italy, September 18-21, 2006. Scientific Board member for an international meeting entitled Kinases, Phosphatases, and Lipases in Health and Disease. Magna Graecia University, Catanzaro, Italy, October 20-21, 2010. Sessions organized/chaired at National/International Symposia Co-organizer and Co-chair (with M.S. Awayda of Tulane University in New Orleans) of Featured Topic Symposium entitled "Regulation of the Epithelial Sodium Channel" as part of Experimental Biology '99, Annual National meeting of the Federation of Societies of Experimental Biology, Washington, D.C., 4/99. Co-organizer and Co-chair (with Kim Barrett, University of California, San Diego) of Featured Topic Symposium entitled "Phosphoinositide Signaling Pathways" as part of Experimental Biology 2000, Annual National meeting of the Federation of Societies of Experimental Biology, San Diego CA, 4/00 Chair of session entitled SGK and ENaC at the International Aldosterone Conference, San Francisco, CA, 5/02 Chair of session on ENaC at the Regulation at Aldosterone and ENaC: From Genetics to Physiology Meeting of the American Physiological Society, Banff, Canada, 9/12/03 Chair of Session Epithelial Ion Channels at the Experimental Biology 2005/ International Union of Physiological Sciences joint international meeting, San Diego, CA, 4/3/05. Chair of Session Mechanism of Transport Across Renal Epithelia at the Joint meeting of the German Society of Physiology and the Federation of European Physiological Societies Munich, Germany 3/29/06 Other recent National and International Service: 2000 Ad hoc grant reviewer, Swiss National Science Foundation 2001

International Examiner for the Doctoral Degrees Board, University of Cape Town, South Africa, for the thesis of M.B. Butterworth - The mechanism of sodium channel trafficking to the apical membrane of kidney epithelial cells, after direct hormonal stimulation. Judge for the Young Investigators Competition International Aldosterone Meeting,

2001

4 Bonnie L. Blazer-Yost Denver. CO 2002 International reviewer - Flemish Science Foundation (Fonds voor Wetenschappelijk Onderzoek Vlaanderen) for a grant entitled ATP release in renal epithelial cells: Relation to cell swelling and intracellular calcium transients P.I. Prof. W. van Driessche 2003 Mentor, American Physiology Society, Minority Fellowship Program at EB2003 meeting 2004 International Review Irish Science Foundation 2006 Ad Hoc reviewer: The Wellcome Trust, London, England BBRC Biotechnology and Biological Sciences, England 2010 External examiner for the mid-term (2nd year) presentations of the Ph.D. candidates in Molecular Oncology and Experimental Immunology, Magna Graecia University, Catanzaro, Italy (10/18/10) Courses Taught: Biology 557 Physiology II - each Spring semester since 1994 Biology 697 - Epithelial Ion Transport and Disease - Seminar - each Fall semester 1994-2005 Biology 556 Physiology I each fall since 2007 Lecturer in team-taught course - G760 Cellular Basis of Renal Function, 1996, 1998 Primary Research Advisor for undergraduates Each student completed two semesters of K493 and one semester of K494 Kerrie Buss Shana Kelly Vedvati Patel Michael Anne Eaves Priya Kheradia Taher Saifullah Jamie Black Holly Brunner Lori Roberts Kimberly Kuebler Ryan Widau Jessica Friedrich Diane Hoover Ryan White Nathan Kast Ryan Widau Ryan Nowrocki Caron Morales

Each of the following students completed 5 semesters of K493 and one semester of K494 to qualify for a research intensive thesis notation Carla Faletti Laura Platt Judith Vahle Adam Amos T. Aaron West Lacey Creason

Major Professor for graduate students: Masters Kimberly Lee Amy Hartman Thomas Lahr Annique Wilson Ph.D. Charity Nofziger Dominika Kopacz Kevin Kowalski Michael Anne Shane Maha Enazy T. Aaron West Monalisa Muchatuta Puneet Souda Stephanie Flagg

Amiraj Banga

Bonnie L. Blazer-Yost Julie Hayden Previous Grants: 1986-89 Co-investigator, Veterans Affairs Merit Review Grant ($402,649) Renal Epithelial Na+ Transport: Role of Proteins P.I. = Malcolm Cox, M.D. 1988-89 1989-90 1990-93 1991-96 1994-95 1994-96 1997-98 1997-99

Principal Investigator, Univ. Penn. Biomedical Research Support Grant ($10,000) Effect of Insulin and Growth Factors on Na+ Transport in Renal Epithelia Principal Investigator, University of Pennsylvania Foundation Award ($10,000) Development and Partial Characterization of a Mammalian Kidney Cell Line Co-investigator, Veterans Affairs Merit Review Grant ($347,335) Renal Epithelial Na+ Transport: Role of Proteins P.I. = Malcolm Cox Principal Investigator, Veterans Affairs Merit Review Grant ($507,692) Renal Epithelial Na+ Transport: Insulin and IGF1 Principal Investigator, National Kidney Foundation of Indiana ($4,500) Characterization of Renal Amiloride-Sensitive Na+ Channels. Principal Investigator, American Heart Association, Grant-In-Aid ($57,104) Characterization of Proteins involved in Aldosterone-regulated Na+ Reabsorption Principal Investigator, National Kidney Foundation of Indiana ($9,600) Regulation of amiloride-sensitive Na+ channel by CFTR in the MDCK-C7 cell line. Principal Investigator, American Heart Association, Grant in Aid ($59,522) Aldosterone regulation of sodium reabsorption in a cell culture model of the distal nephron. Principal Investigator, Lowes Syndrome Foundation ($15,000) The Phosphoinositide pathway in regulation of ion transport phenomena in a renal distal tubule cell line. International Collaborator on grant submitted to the Italian Department of Research and Education. Title: Meccanismi molecolari responsabili dellassociazione fra iperinsulinemia, insulino resistenza ed ipertensione arteriosa. P.I. = Nicola Perrotti. For B. Blazer-Yost, provided travel funds to visit the University of Catanzaro, Italy, for a series of lectures and collaborative interactions with Dr. Perrotti in the summer of 2003. Principal Investigator, GlaxoSmithKline Research Grant. ($624,486). Effects of PPAR agonists on insulin-stimulated Na+ reabsorption in cell culture models of the principal cells of the mammalian distal nephron.

1999-00

2002

2002-2006

6 Bonnie L. Blazer-Yost 2005 - 2006 Principal Investigator, International Development Grant, IUPUI ($15,000) Establish an international exchange program between IUPUI and the University of Catanzaro, Italy. 2005-2006 Principal Investigator, Indiana University School of Medicine Biomedical Research Pilot Grant ($10,000), Importance of the Cellular Cytoskeleton for Hormonestimulated Na+ Transport. Principal Investigator, Purdue University Foundation Research Grant. Stipend support of Ph.D. student Charity Nofziger. Principal Investigator, Deutscher Akademischer Austausch Dienst. Research Visits for University Academics. 2,800 Euro Principal Investigator, International Enhancement Grant, Indiana University ($1,500). The role of serum, glucocorticoid-induced kinase (SGK) in normal renal function and in the pathophysiology of renal fibrosis. Co-Investigator, Italian Ministry of Education, Grant of Internationalization of University System. (60,000 Euro = $76,600). Prof. Nicola Perrotti, P.I., Conference Expenses and Student Research Travel Grant in support of an International Consortium to study the role of SGK in health and disease. Principal Investigator. RSFG ($35,000) Proof of concept testing for the efficacy of thiazolidindiones in inhibiting kidney and liver cyst growth in an animal model of polycystic kidney disease. Travel awards to fund participation in the European Cystic Fibrosis Society meeting in Portugal: Purdue Research Foundation International Travel Grant - $1,000 IU Overseas Conference Fund Grant - $600

2006-2007 2006 2006-2007

2006-2008

2009

2009

Active Grants: 2008-2012 2009-2011 Co-investigator, NIH-RO1 ($240,000/year) Nanoparticle Effects on Epithelial Cell Protein Expression and Function Co-Investigator, NIH 3R01 GM085218-02S1 ($240,000/year) Supplement under the Challenge Grant mechanism for the RO1 listed above. Principal Investigator Membrane Biosciences Signature Center grant ($32,000) Identification of Lipid Components Responsible for Fluid Secretion in Human Autosomal Dominant Polycystic Kidney Disease (ADPKD) Cyst Fluid

2009-2010

7 Bonnie L. Blazer-Yost 2009-2010 Principal Investigator - CTSI grant ($37,417) Proof of concept testing for the efficacy of Rosiglitazone in inhibiting kidney and liver cyst growth in an animal model of autosomal dominant polycystic kidney disease. 2009-2010 Principal Investigator TTR Core Pilot Grant ($9,520) Use of electron microscopy center to study the expression of cystic fibrosis transmembrane regulator in cysts of a rat model of polycystic kidney disease. Mentor - NSF - URM A Multi-year Immersion in Interdisciplinary Research in Biological Signaling, ($943,000) SK Randall, PI

2011-2014

Patents: Co-inventor (with K. Brown; GlaxoSmithKline) of US Utility Patent Application 2008/0113996 entitled Medicaments Sole inventor of PCT/US 2009/056,235 entitled Use of PPAR Modulators to Treat Cystic Liver Disease Senior inventor of the team. Provisional Patent 2009/09014 entitled Treatment of Cystic Disease with Lysophosphatic Acid Antagonists. Recent Lectures by Invitation National and International: Hormonal Regulation of the Epithelial Sodium Channel - ENaC. Physiology Department, Wright State University. (10/24/02) Hormonal Regulation of the Epithelial Sodium Channel - ENaC. Physiology Department, Dartmouth University (12/11/02). Dunaway-Burnham Visiting Fellow. The Dunaway-Burnham Fellowship is presented by the Physiology Department of Dartmouth University each year to recognize outstanding speakers who are leaders in their field. "The Endocrine Selection of the Year". Hormonal regulation of the epithelial sodium channel (ENaC). Dipartimento di Medicina Sperimentale e Clinica, University of Catanzaro, Catanzaro, Italy, (6/03) The genetic component of hypertension. Medical School, University of Catanzaro, Catanzaro, Italy, (6/03) Effect of PPAR agonists on ion transport in a renal principal cell model: Implications for electrolyte and water homeostasis. GlaxoSmithKline, Research Triangle Park, NC, (7/25/04).

Bonnie L. Blazer-Yost

The Genetic Component of Essential Hypertension. University Magna Graecia, Medical School Class on Genetics and Pathology, Catanzaro, Italy (3/16/05). Metabolic Syndrome:Does Hyperinsulinemia Cause Hypertension? University Magna Graecia, Catanzaro, Italy (3/17/05). Insulin-stimulated Na+ transport in renal principal cells: Combining electrophysiology, confocal imaging and proteomics. National Institutes of Health, Bethesda, MD (4/17/05). PPAR-induced edema: Is ENaC-mediated Na+ retention the primary effect? PPARinduced Edema Experts Meeting. Metabolex Corporation. Oakland, CA (8/17/05). Pathways stimulated by mineralocorticoid receptor activation in the renal principal cell: Knowns and unknowns. Eli Lilly, Indianapolis, IN (11/10/05). PPAR agonists and edema: Mechanistic studies in renal cell lines. Cambridge Institute for Medical Research. Addenbrookes Hospital, University of Cambridge, Cambridge, England 6/26/06 PPAR agonists and renal ion transport. Institute of Physiology, University of Tuebingen, Tuebingen, Germany. (7/06) Insulin-stimulated Na+ Transport in Renal Principal Cells. Department of Physiology, University of Regensburg, Regensburg, Germany (7/06) Insulin-stimulated Na+ Transport in Renal Principal Cells Ion Channels and Cell Signalling Research Centre, Division of Basic Medical Sciences, St. Georges, University of London, UK. (7/06). Hormonal Regulation of Renal Sodium Transport Department of Biology, Ball State University, Indiana (11/10/06) Effects of PPAR agonists on renal ion transport: Investigations into the mechanism of agonist effect in the distal nephron. GlaxoSmithKline, Research Triangle Park, North Carolina, (6/15/07). Structural and Functional Analysis of Liver Cyst Epithelia Isolated from the BALB/ccpk/+ Mouse Model of Polycystic Kidney Disease. Institute for Pharmacology and Toxicology, Paracelsus Medical University, Salzburg, Austria (10/2/08). PPAR agonists, renal electrolyte transport and fluid retention. Biomedical Research Institute, Ninewells Hospital and Medical School, Dundee, Scotland (6/7/10). Recent Lectures by Invitation Local:

Bonnie L. Blazer-Yost Metabolic Syndrome: Does hyperinsulinemia cause hypertension? Endocrine Research Seminar series, IUSM, (3/15/04). Metabolic Syndrome: Hyperinsulinemia and hypertension. Department of Cellular and Integrative Physiology, (1/19/05) Metabolic Syndrome: Hyperinsulinemia, hypertension and the kidney. Center for Diabetes Research, IUSM, (5/17/05).

Mentoring Undergraduate Research: Presentation as part of the Workshop entitled: Why Not Both? Undergraduate research as a Powerful Pedagogy for Students and an Aid to Your Own Research Progress. IUPUI Center for Research and Learning (10/14/05). The use of proteomics in the search for unknown proteins in known signaling pathways. Research in Progress series, Nephrology Division, IUSM. (12/19/05). Insulin-sensitizing agents and edema formation. Nephrology Division Research Series, Indiana University School of Medicine. (3/8/06). Insulin-sensitizing agents and edema formation a physiologists perspective. Veterans Administration Medical Center, Indianapolis. (4/13/06). The Epithelial Sodium Channel (ENaC) in Frogs, Mice and Humans. Department of Anatomy and Cell Biology, IUSM (3/20/07). Metabolic Syndrome: Hyperinsulinemia and Hypertension. Center for Diabetes Research, IUSM (4/10/07) Rationale for routine monitoring of blood insulin levels. Roche Diagnostics, Indianapolis, IN. (11/6/08). Membrane Transporters and Polycystic Kidney Disease. Membrane Biosciences in Pathogenesis Symposium sponsored by the Center for Membrane Biosciences. (11/6/09) Insulin sensitizing agents and polycystic kidney disease: A serendipitous observation. Department of Cellular and Integrative Physiology, IUSM (11/10/09) Insulin sensitizing agents and polycystic kidney disease: A serendipitous observation. Eli Lilly and Co. Indianapolis, IN (12/10/09). PPAR agonists and fluid retention. Center for Diabetes Research, Indiana University School of Medicine, Indianapolis, IN (9/9/10) Recent Platform Talks at National and International Meetings:

Bonnie L. Blazer-Yost

10

Effects of PPAR agonists on hormone stimulated ion transport in cell culture models of the principal cells of the mammalian distal nephron. 3rd International Symposium on PPARs efficacy and safety. Monte Carlo, Monaco, (3/21/05). Hyperinsulinemia, Hypertension and the Kidney Are they linked? Annual Indiana University/University of Louisville Nephrology Research Meeting. Indianapolis, IN, (2/25/05). A proteomic approach to studying transepithelial signaling: Insulin stimulation of sodium reabsorption in a model renal cell line. Featured Topics Experimental Biology 2005/ IUPS, San Diego, CA, (4/2/05). Hormonal Control of Sodium Reabsorption: From Bufo marinus bladder to Mus musculus cortical collecting duct. Symposium entitled Hormonal Control of Salt and Water Balance in Vertebrates held at the 15th International Congress of Comparative Endocrinology. Boston, MA, (5/25/05). Studies of Sgk1 Function in Renal Principial Cells: Role of the Kinase in Hormone Stimulated Na+ Reabsorption M.I.U.R. Interlink Symposium Using Rodent Models to Study Liver and Kidney Disease. Catanzaro, Italy (9/18/06). Functional analysis of biliary cyst epithelia combined with proteomic analysis of cyst fluid isolated from the BALB/c-cpk/+ model of polycystic kidney disease. 6th International Symposium on Cell Volume Regulation in Health and Disease. Salzburg Austria 9/24/07 Structural and Functional Analysis of Liver Cyst Epithelia Isolated From the BALB/ccpk/+ Mouse Model of Polycystic Kidney Disease. Midwest Biological Transport Group of the American Physiological Society, Lake Cumberland Kentucky (6/22/08) Regulation of renal tubular transport by insulin. 39th Annual German Congress for Nephrologie, Tuebingen, Germany (9/28/08). Inhibition of Renal CFTR by Insulin-Sensitizing Agents: Implications for a Central Role for CFTR in Renal-Based Pathologies of Fluid Balance. European Cystic Fibrosis Society New Frontiers in Basic Science of Cystic Fibrosis. Tavira, Portugal. (4/19/09) Effect of Pioglitazone on cystic disease in the PCK rat model of polycystic kidney disease. Midwest Biological Transport Group of the American Physiological Society, Lake Cumberland Kentucky (6/23/09) Pioglitazone attenuates the progression of kidney and liver disease in the PCK rat model of polycystic kidney disease. Midwest Biological Transport Group of the American Physiological Society, Lake Cumberland Kentucky (6/22/10) The Importance of International Collaboration. M.I.U.R. Interlink Symposium Kinases, Phosphates and Lipases in Health and Disease, Catanzaro, Italy (10/22/10) Bioactive Lipids in Cyst Fluid From Polycystic Kidney Disease Patients. M.I.U.R.

11 Bonnie L. Blazer-Yost Interlink Symposium Kinases, Phosphates and Lipases in Health and Disease, Catanzaro, Italy (10/22/10) Original Papers 1. States, B., B. Blazer and D. Harris. Prenatal diagnosis of cystinosis. J. Pediatr. 87:558-562, 1975. 2. Cohn, R.M., M. Yudkoff, B.L. Yost and S. Segal. Phenylalanine-tyrosine deficiency syndrome as a complication of the management of hereditary tyrosinemia. Am. J. Clin. Nutr. 30:209-214, 1977. 3. Yudkoff, M., B. Blazer-Yost, R. Cohn and S. Segal. On the clinical significance of the plasma alpha-amino-n-butyric acid:leucine ratio. Am. J. Clin. Nutr. 32:282-285, 1979. 4. Steiman, J., M. Yudkoff, B. Blazer-Yost and P. Berman. Late onset nonketotic hyperglycemia: A disorder of glycine transport. J. Pediat. 94:907-911, 1979. 5. Bovee, K.C., T. Joyce, B.L. Blazer-Yost, M.A. Goldschmidt and S. Segal. Characterization of renal defects of Fanconi syndrome in dogs. J. Am. Vet. Med. Assn. 174:1094-1099, 1979. 6. Blazer-Yost, B.L. and P.F. Jezyk. Free amino acids in the plasma and urine of dogs from birth to senescence. Am. J. Vet. Res. 40:832-838, 1979. 7. Blazer-Yost, B., R.A. Reynolds and S. Segal. Amino acid content of rat renal cortex and the response to in vitro incubation. Am. J. Physiol. 236 (Renal Fluid Electrolyte Physiol 5): F398404, 1979. 8. Burch, J.W. and B.L. Blazer-Yost. Acetylation of albumin by low doses of aspirin. Thrombosis Res. 23:447-452, 1981. 9. Blazer-Yost, B.L., M. Geheb, A. Preston, J. Handler and M. Cox. Aldosterone-induced proteins in renal epithelia. Biochim. Biophys. Acta 719:158-161, 1982. 10. Blazer-Yost, B.L. and M. Cox. Aldosterone-induced proteins: Characterization using lectinaffinity chromatography. Am J. Physiol. 249 (Cell Physiol. 18): C215-225, 1985. 11. Baird, A.W., W.S. Barclay, B.L. Blazer-Yost and A.W. Cuthbert. Affinity purified IgG transfers immediate hypersensitivity to guinea-pig colonic epithelium in vitro. Gastroenterology 92:635-642, 1987. 12. Blazer-Yost, B.L. and M. Cox. Insulin-like growth factor 1 stimulates renal epithelial Na+ transport. Am. J. Physiol. 255 (Cell Physiol. 24): C413-417, 1988. 13. Blazer-Yost, B.L., M. Cox and R. Furlanetto. Insulin and IGF1 receptor-mediated Na+ transport in toad urinary bladders. Am. J. Physiol. 257 (Cell Physiol. 26): C612-620, 198

Bonnie L. Blazer-Yost

12

14. Szerlip, H., P. Palevsky, M. Cox and B. Blazer-Yost. Relationship of the aldosteroneinduced protein GP70, to the conductive Na+ channel. J. Am. Soc. Nephrol. 2:1108-1114, 1991. 15. Blazer-Yost, B.L., M. Watanabe, T.P. Haverty and F.N. Ziyadeh. Role of insulin and IGF1 receptors in proliferation of cultured renal proximal tubule cells. Biochim. Biophys Acta 1133:329-335, 1992. 16. Blazer-Yost, B.L., Y. Fesseha and M. Cox. Aldosterone-mediated Na+ transport in renal epithelia: Time course of induction of a potential regulatory component of the conductive Na+ channel. Biochem. Internat. 26:887-897, 1992. 17. Blazer-Yost, B.L., N. Shah, L. Jarett, M. Cox and R.M. Smith. Insulin and IGF1 receptors in a model renal epithelium: Receptor localization. Biochem. Internat. 28:143-153, 1992. 18. Coupaye-Gerard, B., H.J. Kim, A. Singh and B.L. Blazer-Yost. Differential effects of Brefeldin A on hormonally regulated Na+ transport in a model renal epithelial cell line. Biochim. Biophys. Acta 1190:449-456, 1994. 19. Blazer-Yost, B.L., R.D. Record and H. Oberleithner. Characterization of hormonestimulated Na+ transport in a high-resistance clone of the MDCK cell line. Pflugers Archiv. Eur. J. Physiol. 432:685-691, 1996. 20. Record, R.D., M. Johnson, S. Lee and B.L. Blazer-Yost. Aldosterone and insulin stimulate amiloride-sensitive sodium transport in A6 cells by additive mechanisms. Am. J. Physiol. 271 (Cell Physiol. 40): C1079-1084, 1996. 21. Bindels, R.J.M., A.M.T. Engbersen, A. Hartog and B.L. Blazer-Yost. Aldosterone-induced proteins in primary cultures of rabbit renal cortical collecting system. Biochim Biophys. Acta. 1284:63-68, 1996. 22. Blazer-Yost, B.L. and S.I. Helman. The amiloride-sensitive epithelial Na+ channel: Binding sites and channel densities. Am. J. Physiol. 272 (Cell Physiol. 41): C761-769, 1997 23. Helman, S.I. and B.L. Blazer-Yost Reply to Letter to the Editor from Prof. A. W. Cuthbert (regarding the review article published in Am. J. Physiol. 272:C1928-1935, 1997) Am. J. Physiol. 273 (Cell Physiol.42): C1437-1439, 1997. 24. Blazer-Yost, B.L., C. Hughes and P. Nolan. Protein prenylation is required for aldosteronestimulated Na+ transport. Am.J. Physiol. 272: (Cell Physiol. 41): C1928-1935, 1997. 25. Helman, S.I., X. Liu, K. Baldwin, B. Blazer-Yost and W. J. Els. Time-dependent stimulation by aldosterone of blocker-sensitive ENaCs in A6 epithelia. Am. J. Physiol. 274 (Cell Physiol. 43): C947-957, 1998. 26. Record, R.D., L. Froelich, C.J. Vlahos and B.L. Blazer-Yost. Phosphatidylinositol 3-kinase

13 Bonnie L. Blazer-Yost activation is required for insulin-stimulated sodium transport in A6 cells. Am. J. Physiol. 274 (Endocrin. Metabol. 37):E611-617, 1998 27. Blazer-Yost, B.L., X. Liu and S.I. Helman. Hormonal regulation of ENaCs: Insulin and aldosterone. Am. J. Physiol. 274 (Cell Physiol.43):C1373-1379, 1998 28. Blazer-Yost, B.L., T.G. Paunescu, S.I. Helman, K.D. Lee and C.J. Vlahos. Phosphoinositide 3-kinase is required for aldosterone regulated sodium reabsorption. Am. J. Physiol. 277 (Cell Physiol. 46): C531-536, 1999. 29. Lahr, T.F., R.D. Record, D.K. Hoover, C.L. Hughes and B.L. Blazer-Yost. Characterization of the ion transport responses to ADH in the MDCK-C7 cell line. Pflugers Arch. - Eur. J. Physiol. 439:610-617, 2000. 30. Paunescu, T.G., B.L. Blazer-Yost, C.J. Vlahos, and S.I. Helman. Involvement of an LY294002 inhibitable PI3-kinase in regulation of baseline rates of Na+ transport in A6 epithelia. Am. J. Physiol. Cell Physiol. 279:C236-247, 2000 31. Blazer-Yost, B.L., M. Butterworth, A.D. Hartman, G.E. Parker, C.J. Faletti, W.J. Els, and S.J. Rhodes. Characterization and imaging of A6 epithelial cell clones expressing fluorescently labeled ENaC subunits. Am. J. Physiol. Cell Physiol. 281:C624-632, 2001. 32. Faletti, C.J., N. Perrotti, S.I. Taylor and B.L. Blazer-Yost. sgk: An essential convergence point for peptide and steroid hormone regulation of ENaC-mediated Na+ transport. Am. J. Physiol. Cell Physiol. 282:C494-500, 2002. 33. Blazer-Yost, B.L., M. A. Esterman and C.J. Vlahos. Insulin-stimulated trafficking of ENaC in renal cells requires PI 3-kinase activity. Am. J. Physiol. Cell Physiol. 284: C1645-1653, 2003. 34. Blazer-Yost, B.L., J.C. Vahle, J.M. Byars and R. Bacallao. Real-time three dimensional imaging of lipid signal transduction: Apical membrane insertion of epithelial Na+ channels. Am. J. Physiol. Cell Physiol. 287:C1569-C1576, 2004 35. Blazer-Yost, B.L. and C. Nofziger. The role of the phosphoinositide pathway in hormonal regulation of the epithelial sodium channel. Adv. Expert. Med. & Biol. 559:359-368, 2004. 36. Blazer-Yost, B. L. and C. Nofziger. Phosphoinositide lipid second messengers: New paradigms for transepithelial signal transduction. Pflugers Archiv. Eur. J. Physiol. 450:75-82, 2005. 37. Blazer-Yost, B.L., T. A. West, J. Stack, K. Peck, T.F. Lahr and M. Gekle. Effect of the mycotoxin, Ochratoxin A, on hormone-stimulated ion transport in a cultured cell model of the renal principal cell. Pflugers Arch. Eur. J. Physiol. 450: 53-60, 2005. 38. Wagner, M.C., B.L. Blazer-Yost, J. Boyd-White, A. Srirangam, J. Pennington and S. Bennett. Expression of the Unconventional Myosin, Myo1c Alters Sodium Transport in M1

Bonnie L. Blazer-Yost Collecting Duct Cells. Am. J. Physiol. Cell Physiol. 289: C120-129, 2005.

14

39. West, T.A. and B.L. Blazer-Yost. Modulation of basal and peptide hormone-stimulated Na+ transport by membrane cholesterol content in the A6 epithelial cell line. Cell. Physiol. Biochem. 16:263-270, 2005 40. Nofziger, C. L. Chen, M. A. Shane, C. Smith, K. Brown and B.L. Blazer-Yost. PPAR agonists do not modulate insulin-stimulated sodium reabsorption in the A6, M1 or mpkCCDcl4 renal cell lines. Pflugers Arch. Eur. J. Physiol. 451:445-453, 2005. 41. Shane, M.A., C. Nofziger, and B.L. Blazer-Yost. Hormonal regulation of epithelial Na+ channel: From amphibians to mammals. J. Gen. Compara. Endocrin. 14: 85-92, 2006. 42. Artunc, F. D. Sandulache, O. Nasir, K.M. Boini, B. Friedrich, N. Beir, E. Dicks, S. Potzsch, K. Klingel, V. Vallon, B.L. Blazer-Yost, W. Scholz, T. Risler, D. Kuhl and F. Lang. Lack of the serum and glucocorticoid inducible kinase SGK1 attenuates the volume retention following treatment with the PPAR agonist pioglitazone. Pflugers Arch. Eur. J. Physiol. 456:425-436, 2008. 43. Lai, X., R.L. Bacallao, B. L. Blazer-Yost, D. Hong, S.B. Mason and F.A.Witzmann. Characterization of the renal cyst fluid proteome in Autosomal dominant polycystic kidney disease (ADPKD) patients. Proteomics Clin. Appl. 2:1140-1152, 2008. 44. Sivasankar, M. C. Nofziger and B.L. Blazer-Yost. cAMP Regulation of Ion Transport in Porcine Vocal Fold Mucosa. Laryngoscope 118:1511-1517, 2008 46. Nofziger, C., K. Kalsi, T. A. West, D. Baines and B. Blazer-Yost. Vasopressin regulates the phosphorylation state of AMP-activated protein kinase (AMPK) in MDCK-C7 cells. Cellular Physiol. Biochem. 22:487-496, 2008. 45. Muchatuta, M., V. Gattone, F. Witzmann, B.L. Blazer-Yost. Structural and Functional Analysis of Liver Cysts from the BALB/c-cpk Mouse Model of PKD. Exp. Biol. Med. 234:1727, 2009 47. Sivasankar, M., B. L. Blazer-Yost, Long-acting beta agonists increase vocal fold ion transport. Laryngoscope 119:602-607, 2009 48. Lai, X., B.L. Blazer-Yost, V.H. Gattone, D. Hong, M.N. Muchatuta and F.A. Witzmann. Protein composition of liver cyst fluid from the BALB/c-cpk/+ mouse model of autosomal recessive polycystic kidney disease (ARPKD). Proteomics 9:3775-3782 2009 49. Garrone, N.F., B.L. Blazer-Yost, R.B. Weiss, J-M. Lalouel, A. Rohrwasser. A human polymorphism affects Nedd41 subcellular targeting by leading to two isoforms that contain or lack a C2 domain. BMC Cell Biology 10:26, 2009 50. Nofziger, C., K.K. Brown, C.D. Smith, W. Harrington, D. Murray, J. Bisi, T.T. Ashton, F.P.

15 Bonnie L. Blazer-Yost Maurio, K. Kalsi, T.A. West, D. Baines and B.L. Blazer-Yost. PPAR agonists inhibit vasopressin mediated anion transport in the MDCK-C7 cell line. Am. J. Physiol: Renal Physiol. 297: F55-62, 2009. 51. Mason, S.B., X. Lai, R.L. Bacallao, B.L. Blazer-Yost, V. H. Gattone, K.C. Wang, F.A. Witzmann. The biomarker enriched proteome of autosomal dominant polycystic kidney disease cyst fluid. Proteomics Clin. Appl. 3:1247-1250, 2009. 52. Nofziger, C. and B.L. Blazer-Yost. PPAR Agonists, Regulation of Renal Ion Transporters, and Fluid Retention. J. Am. Soc. Nephrol. 20: 2481-2483, 2009. 53. Blazer-Yost, B.L. PPAR Agonists Blood Pressure and Edema. PPAR Research Volume 2010, Article ID 785369 (electronic journal), 2010. 54. Menniti, M., R. Iuliano, M. Sopjani, M. Foller, S. Mariggio, C. Nofziger, A.M. Perri, R. Amato, B. Blazer-Yost, D. Corda, F. Lang and N. Perrotti. 60 kDa lysophospholipase, a new Sgk1 molecular partner involved in the regulation of ENaC. Cell. Physiol. Biochem. 26:587596, 2010 55. Blazer-Yost, B.L., A. J. Banga, A. Amos, E. Chernoff, X. Lai, C. Li, S. Mitra and F.A. Witzmann. Effect of carbon nanoparticles on barrier function of renal epithelial cells. Nanotoxicology In press 56. Blazer-Yost, B.L., J. Haydon, T. Eggleston-Gulyas, J-H. Chen, X. Wang, V. Gattone and V.E. Torres. Pioglitazone attenuates cystic burden in the PCK rodent model of polycystic kidney disease. PPAR Research. ID 274376 (electronic journal), 2010 57. Banga, A. F.A. Witzmann and B.L. Blazer-Yost. Functional effects of nanoparticle exposure on Calu-3 airway epithelial cells. Nanomedicine: Nanotechnology, Biology and Medicine. Submitted Chapters Blazer-Yost, B.L., S. Goldfarb and F.N. Ziyadeh. Insulin, insulin-like growth factors and the kidney. in Hormones, Autocoids and the Kidney. Contemporary Issues in Nephrology Vol. 23, S. Goldfarb and F.N. Ziyadeh, Eds. Churchill Livingstone, N.Y. 1991.

Hua-Chen Chang, CV 2010

Curriculum Vitae Hua-Chen Chang, Ph.D.

Assistant Professor Department of Biology, School of Science Indiana University-Purdue University Indianapolis (IUPUI) 723 West Michigan Street, SL310, Indianapolis, IN 46202 Email: huchang@iupui.edu TEL: 317-278-3897 EDUCATION AND TRAINING 1991 Bachelor of Science MICROBIOLOGY National Chung-Hsing University, Taichung, Taiwan Master of Science VETERINARY PATHOBIOLOGY Purdue University, West Lafayette, IN Doctor of Philosophy VETERINARY PATHOBIOLOGY Purdue University, West Lafayette, IN Post-Doctoral Fellow MICROBIOLOGY & IMMUNOLOGY Indiana University School of Medicine, Indianapolis, IN

1996

2000

2000-2006

ACADEMIC APPOINTMENTS 2006 2009 Assistant Research Professor DEPARTMENT OF PEDIATRICS HB Wells Center for Pediatric Research Indiana University School of Medicine, Indianapolis, IN Assistant Professor DEPARTMENT OF BIOLOGY School of Science Indiana University-Purdue University Indianapolis, IN

2009 now

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Hua-Chen Chang, CV 2010

HONORS AND AWARDS 1998 B.S. Pomeroy Award for Student Achievement in Avian Disease Research at the 49th North Central Avian Disease Conference and Symposium on Enteric and Emerging Diseases Basic Science Research Poster Award from the Society of Phi Zeta

2000

PROFESSIONAL ORGANIZATIONS Associate member of Indiana University Simon Cancer Center (Hematopoiesis, Microenvironment and Immunology). Member of American Association of Immunologists (AAI).

TEACHING ASSIGNMENTS CLASSROOM INSTRUCTION: Lecturer, BIOL561 Immunology (Graduate Level Course), Department of Biology, Indiana University-Purdue University Indianapolis, enrollment 79, 3Cr./Spring Semester 2010. Lecturer, BIOL697 Immune System Disorder (28770, Graduate Level Course), Department of Biology, Indiana University-Purdue University Indianapolis, enrollment 24, 3Cr/Fall Semester 2010. Lecturer, Current Topics in Immunology J807 (Graduate Level Course): One lecture on the Tumor immunity; Indiana University School of Medicine, Department of Microbiology and Immunology, 2008, 2009, and 2010. GRADUATE SCHOOL THESIS COMMITTEES: 2008-present 2009-present 2010-present Yung-Yi Chen, PhD (candidate), Department of Comparative Pathobiology, Purdue University, Committee Member Ivan Petrov Lupov, MS (candidate), Department of biology, IUPUI, Chair of the Committee Larry Voiles, MS (candidate), Department of biology, IUPUI, Chair of the Committee

GRADUATE STUDENTS: 2009-present 2010-present

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Ivan Petrov Lupov, MS (candidate) Larry Voiles, MS (candidate)

Hua-Chen Chang, CV 2010

GRADUATE RESEARCH NON-THESIS ADVISEES: K595 2009-2010 Manuel Carlos De La Rosa Jr, MS (candidate) Department of Biology, IUPUI 2009-2010 2009-2010 Kinnari J Oza, MS (candidate) Department of Biology, IUPUI Allysia Schwartz, MS (candidate) Department of Biology, IUPUI

UNDERGRADUATE RESEARCH ADVISEES: K493/494 Senior Research Project 2009-2010 Cameron Walter/ BS; Department of Biology, IUPUI 2009-2010 Larry Voiles/ BS; Department of Biology, IUPUI 2009-2010 Adam Hesse/ BS (candidate); Department of Biology, IUPUI 2010-present Anya Lawrence-Cooper/ BS (candidate); Department of Biology, IUPUI 2010-present Bailey Anderson/ BS (candidate); Department of Biology, IUPUI 2010-present Lonya Semenovich Melnikov / BS (candidate); Department of Biology, IUPUI 2010-present Kennedy Obeime / BS (candidate); Department of Biology, IUPUI K490 Senior capstone paper 2009-2010 Puja Patel/ BS; Department of Biology, IUPUI 2010-present Kennedy Obeime / BS (candidate); Department of Biology, IUPUI

OTHER STUDENT MENTORING/ADVISING: The Indianapolis Project SEED 2010 (summer) Nail Siddeeq/ Arsenal Technical High School Indianapolis, IN PROFESSIONAL SERVICE Manuscript Reviewer: 2009-present: Biochemical Pharmacology 2010-present Current Chemical Biology (CCB) UNIVERSITY SERVICE Department: 2009-2010
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Undergraduate Recruiting

Hua-Chen Chang, CV 2010

2010-present 2010 Graduate Recruiting Forensic Biology Search Committee

INVITED SEMINARS/PRESENTATIONS 2009 Aberrant DNA methylation for early detection of lung cancer, Lung Cancer Working Group, IU School of Medicine Impaired Th1 development resulting from chemotherapy-induced regulation of STAT4 in lymphoma patients, Department of Microbiology & Immunology, IU School of Medicine The role of type VI collagen in lung disease, Indiana Center for Vascular Biology and Medicine, VA Medical Center, IU school of medicine

2010

2010

POSTER PRESENTATION 2010 Larry Voiles, Ling Han, Ellen Chernoff, Christoph A. Naumann, David Pelloso, Michael Robertson and Hua-Chen Chang. Immunotherapy for Non-Hodgkins Lymphoma via Nanoparticle-mediated Delivery System. IUPUI Campus Research Day, Indianapolis, IN 2010 Cameron Walter, Adam Hesse, Ling Han, Baohua Zhou and Hua-Chen Chang. Role of TSLP on alveolar macrophages in lung inflammation. IUPUI Campus Research Day, Indianapolis, IN 2010 Ivan Lupov, Ling Han, Manuel De La Rosa, Kinnari Oza, Allysia Schwartz, Ninah Clegg, David Pelloso, Michael Robertson and Hua-Chen Chang. Chemotherapyinduced regulation of STAT4 in lymphoma patients. IUPUI Campus Research Day, Indianapolis, IN 2010 Nail Siddeeq, Ling Han, Larry Voiles, Ivan Lupov and Hua-Chen Chang. The Indianapolis Project SEED, Indianapolis, IN 2010 Ivan Lupov, Ling Han, Larry Voiles, Manuel De La Rosa, Kinnari Oza, Allysia Schwartz, Ninah Clegg, David Pelloso, Michael Robertson and Hua-Chen Chang. Chemotherapy-induced regulation of STAT4 in lymphoma patients. 39th Annual Autumn Immunology Conference, Chicago, IL GRANTS, FELLOWSHIPS AND AWARDS Ongoing:

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Hua-Chen Chang, CV 2010

Enhancement of immunotherapy for Non-Hodgkins lymphoma patients, Pilot Funding for Research Use of Core Facilities, IUSM, 7/1/2007 6/30/2011, The major goal is to simultaneously sort distinct cell populations in PBMC samples obtained from NHL patients using the BD FACSAria sorter for analysis of STAT4 expression. Role: PI Immunotherapy for Non-Hodgkins lymphoma via nanoparticle-mediated delivery system, Indiana University/Purdue University Collaboration in Biomedical Research Pilot Grant Program, IUSM, 3/1/2008 12/31/2010, The goal is to rescue the expression of STAT4/IFN in CD4+ T lymphocytes of NHL patient PBMCs by a nanoparticle delivery system. Role: PI Genome-wide CpG methylation profiling: from chronic inflammation to lung cancer, Lung Cancer Working Group, IUSM, 8/1/2008 12/31/2010, The goal is to obtain a genome-wide DNA methylation profile by pyrosequencing from inflamed lung epithelium cells and the adenocarcinomas that subsequently developed. Role: PI Mechanisms of STAT4 Deficiency in the Immune System of Cancer Patients, RO1 CA118118, NIH/NCI, 7/1/2007 6/30/2012, The major goals of this project are to determine the molecular mechanisms of STAT4 deficiency in patients with cancer and to develop clinically feasible approaches to circumvent this deficiency during immunotherapy for cancer. M.J. Robertson, Principal Investigator Role: Co-PI Completed: Role of Type VI Collagen in the pathogenesis of emphysema, Purdue Research Foundation (PRF) Summer Faculty Grant, Purdue University, 6/1/2010 07/31/2010, The goal is to understand how collagen type VI contributes to the inflammation in the emphysematous lung. Role: PI Enhancement of immunotherapy for cancer patients, Showalter Trust Funds, IUSM, 7/1/2007 6/30/2009, The goal of this proposal is to enhance the efficacy of anti-tumor therapy for cancer patients by rescuing STAT4 and IFN- deficiency. Role: PI Immunotherapy for Non-Hodgkins lymphoma via nanoparticle-mediated delivery system Oncological Sciences Center in Discovery Park, Purdue University, 3/1/2008 04/31/2009. Role: PI

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Hua-Chen Chang, CV 2010

Pending: Type VI collagen in emphysema, R15, NIH/NHLBI, 07/01/2011 - 06/31/2013, The goal is to study the effects of type VI collagen in the pathogenesis of emphysema. Role: PI Submitted but not funded: Molecular mechanisms of STAT4 regulation, Greater Midwest Affiliate Scientist Development Grant, American Heart Association, 2009, Role: PI Mechanisms of Immune Modulation by Botanical Products and Their Derivatives, R21, NIH/NCCAM, 2009, (M.J. Robertson, Principal Investigator) Role: Co-PI STAT4 in atherosclerosis, R15, NIH/ NHLBI, 2009 Role: PI (score 43) Role of TSLP on alveolar macrophages in lung inflammation, RSFG, IUPUI, 2009 Role: PI A TSLP transgenic mouse model for lung adenocarcinoma, DRIVE, IUPUI, 2009 Role: PI CpG methylation events and lung cancer prevention, American Cancer Society Institutional Research Grant, IUSM, 2009 Role: PI Role of Th2 inflammation in lung adenocarcinoma, Concern Foundation, 2010 Role: PI Modulating antitumor immunity by lunasin, R21, NIH/NCI, The goal is to study if soybean peptide lunasin promotes antitumor immunity as a mechanism of cancer prevention. Role: PI PUBLICATIONS Original Research (Peer Reviewed) 1. Lai MY, H.-C. Chang, Li HP, Ku CK, Chen PJ, Sheu JC, Huang GT, Lee PH, and Chen DS. (1993) Splicing mutations of the p53 gene in human hepatocellular carcinoma. Cancer Research 53: 1653-1656.

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Hua-Chen Chang, CV 2010

2. Chang H.-C., Lin T-L, Wu C-C. (2001). DNA-mediated vaccination against infectious bursal disease in chickens. Vaccine. 20:328-335. 3. Chang H.-C., Zhang S and Kaplan MH. (2002). Neonatal tolerance in the absence of Stat4- and Stat6-dependent T helper cell differentiation. J. Immunol. 169:4124-4128. 4. Chang H.-C., Lin TL and Wu CC. (2003). DNA vaccination with plasmids containing various fragments of large segment genome of infectious bursal disease virus. Vaccine. 21:507-513. 5. Kaplan MH, H.-C. Chang, Cooper SC, Lee Y and Broxmeyer HE. (2003) Distinct requirements for Stat4 and Stat6 in hematopoietic progenitor cell responses to growth factors and chemokines. J. Hematother. Stem Cell Res. 12:401-408. 6. Chang H.-C., Zhang S, Oldham IL, Naeger L, Hoey T and Kaplan MH. (2003) The Stat4 N-terminal domain is required for efficient phosphorylation. J. Biol Chem. 278:32471-32477. 7. OSullivan AC, H.-C. Chang, Yu Q and Kaplan MH. (2004) STAT4 is required for IL12 induced chromatin remodeling of the CD25 locus. J Biol Chem. 279:7339-7345. 8. Chang H.-C., Zhang S, Thieu, V, Slee RB, Bruns HA, Laribee RN, Klemsz MJ and Kaplan MH. (2005) PU.1 expression delineates heterogeneity in Th2 cells. Immunity. 22:693-703. 9. Robertson, MJ, H.-C. Chang, Pelloso D, and Kaplan MH. (2005) Impaired interferongamma production as a consequence of STAT4 deficiency after autologous hematopoietic stem cell transplantation for lymphoma. Blood. 106:963-70. 10. Mathur AN, H.-C. Chang, Zisoulis DG, Kapur R, Belladonna ML, Kansas GS, and Kaplan MH. (2006) T-bet is a critical determinant in the instability of the IL-17secreting T-helper phenotype. Blood 108(5): 1595-601. 11. Mathur*, A. N., H.-C. Chang*, D. G. Zisoulis, G. L. Stritesky, Q. Yu, T. O'Malley J, R. Kapur, D. E. Levy, G. S. Kansas, and M. H. Kaplan. (2007). Stat3 and Stat4 Direct Development of IL-17-Secreting Th Cells. J Immunol 178:4901-4907. (*equal contribution) 12. Kaplan, M. H., S. Sehra, H.-C. Chang, J. T. O'Malley, A. N. Mathur, and H. A. Bruns. 2007. Constitutively active STAT6 predisposes toward a lymphoproliferative disorder. Blood 110:4367-4369. 13. Li, Y., J. Yan, P. De, H.-C. Chang, A. Yamauchi, K. W. Christopherson, 2nd, N. C. Paranavitana, X. Peng, C. Kim, V. Munugulavadla, R. Kapur, H. Chen, W. Shou, J. C. Stone, M. H. Kaplan, M. C. Dinauer, D. L. Durden, and L. A. Quilliam. 2007. Rap1a null mice have altered myeloid cell functions suggesting distinct roles for the closely related rap1a and 1b proteins. J Immunol 179:8322-8331.
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Hua-Chen Chang, CV 2010

14. Mann BA Huang JH, Li P, H.-C. Chang, Slee RB, OSullivan A, Mathur A, Yeh N, Klemsz MJ, Brutkiewicz RR, Blum JS and Kaplan MH. 2008. Vaccinia virus infection blocks Stat1-dependent and independent gene expression induced by type I and type II interferons. Journal of Interferon and Cytokine Research 28:367-380. 15. Yu Q, H.-C. Chang, Ahyi AN, Kaplan MH. Transcription factor-dependent chromatin remodeling of Il18r1 during Th1 and Th2 differentiation. J Immunol. 2008;181:3346-3352. 16. John T. O'Malley, Rajaraman D. Eri, Gretta L. Stritesky, Anubhav N. Mathur, H. C. Chang, Harm HogenEsch, Mythily Srinivasan and Mark H. Kaplan. STAT4 isoforms differentially regulate Th1 cytokine production and the severity of inflammatory bowel disease. J Immunol. 2008;181:5062-5070. 17. Vivian T. Thieu*, Qing Yu*, H.-C. Chang *, Norman Yeh, Evelyn T. Nguyen, Sarita Sehra and Mark H. Kaplan. 2008. Signal transducer and activator of transcription 4 is required for the transcription factor T-bet to promote T helper 1 cell-fate determination. Immunity. 2008;29:679-690. (*equal contribution) 18. John T. OMalley, Sarita Sehra, Vivian T. Thieu, Qing Yu, H.-C. Chang, Gretta L. Stritesky, Evelyn T. Nguyen, Anubhav N. Mathur, David E. Levy, and Mark H. Kaplan. 2009. Signal transducer and activator of transcription 4 limits the development of adaptive regulatory T cells. Immunology 127:587-595. 19. H.-C. Chang *, Ling Han, Ritobrata Goswami, Evelyn T. Nguyen, David Pelloso, Michael J. Robertson, and Mark H. Kaplan*. 2009. Impaired development of human Th1 cells in patients with deficient expression of STAT4. Blood 113:5887-5890. (*corresponding author) 20. Ahyi, A. N., H.-C. Chang, A. L. Dent, S. L. Nutt, and M. H. Kaplan. 2009. IFN Regulatory Factor 4 Regulates the Expression of a Subset of Th2 Cytokines. J Immunol 183:1598-1606. 21. Chang H.-C., L. Han, R. Jabeen, S. L. Nutt, and M. H. Kaplan. 2009. PU.1 regulates TCR expression by modulating GATA-3 activity. J Immunol 183:4887-4894. 22. Chang HC, Sehra S, Goswami R, Yao W, Yu Q, Stritesky GL, Jabeen R, Han L, Nguyen ET, McKinley CD, Tepper RS, Robertson MJ, Perumal NB, Nutt SL and Kaplan MH. 2010. The transcription factor PU.1 is required for the development of IL-9-secreting T cells and allergic inflammation. Nature Immunology. 11:527-534.

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CURRICULUM VITAE

ELLEN A.G. CHERNOFF CITIZENSHIP: U.S.A. OFFICE ADDRESS:

Associate Professor of Biology

Indiana University-Purdue University Indianapolis IUPUI Department of Biology (SL 360) and IU Center for Regenerative Biology and Medicine 723 West Michigan Street Indianapolis, IN 46202-5132 Telephone: (317) 274-0591 Fax: (317) 274-2846 email: echernof@iupui.edu

EDUCATION B.A. Ph.D. 1973 1978 University of Chicago Honors in Biology Research Advisor: Professor Jane H. Overton

University of Chicago Department of Biology POSTDOCTORAL RESEARCH EXPERIENCE

University of Pennsylvania, Department of Anatomy: 1978-1979 Temple University Department of Biology:1979-1980 Case Western Reserve Univ. Dept. of Molec. Biol. and Microbiol.: 1980-1983 Case Western Reserve Univ. Dept. of Developmental Genetics and Anatomy: 1983-1986 -Non-tenure track faculty 1986 ACADEMIC APPOINTMENTS Indiana UniversityPurdue University at Indianapolis Indiana UniversityPurdue University at Indianapolis OTHER APPOINTMENTS Member, Paul and Carole Stark Neuroscience Research Institute: Indiana University School of Medicine, IUPUI, 2003-present Assistant Professor Department of Biology 1986-1992

Associate Professor Department of Biology

1992-present

Director, IU Center of Excellence in Regenerative Biology and Medicine (IUPUI) 2001-2005 Sabbatical Visiting Scientist, Elanco/Lilly Research Laboratories, Developmental Biology Group, 1995-1996 Adjunct Faculty, Medical Neuroscience Program, Indiana University School of Medicine, 1992present Visiting Scientist, Eli Lilly Corp., CNS Research Group, 1988 PROFESSIONAL MEMBERSHIPS AAAS: 1982-present Sigma Xi: 1973-present Society for Cell Biology: 1975-1992; 2001 Society for Developmental Biology: 1985-present Society for the Study of Amphibians and Reptiles: 1999-present FELLOWSHIPS (competitive only) AND HONORS (* = in rank)

*Marcus Singer Medal 1997 for furthering research in regeneration and developmental biology
American Men and Women in Science, 1984-present American Heart Association, Northeast Ohio Affiliate, Inc. Post-doctoral Fellowship: 1982-1984 University of Chicago, University Pre-doctoral Fellowship: 1973-1974 (one of 4 awarded to the Division of Biology Sciences and the Pritzker School of Medicine) Francis Bundick Scholarship for Biochemistry/Biology, Undergraduate: 1972-1973 MEMBERSHIPS IN PROFESSIONAL SOCIETIES American Association for the Advancement of Science American Society for Cell Biology Sigma Xi Society for Developmental Biology

TEACHING (in rank) A. COURSE ASSIGNMENTS

For most of my academic career I have taught two sections of K333 and one section of K331 in Spring and Biology 566 in Fall Regular Assignments K331, Embryology, Undergraduate -Principles of animal developmental biology from the morphological through molecular mechanism level. Revised yearly K333, Embryology Laboratory, Undergraduate -Live material experimental developmental biology course consisting of laboratories at the tissue, cellular and subcellular level written by Dr. Chernoff. Includes reading of primary research articles Experiments added and changed as needed. 566, Developmental Biology, Graduate -Major topics changed every three years, updated yearly. Taught entirely from primary research and review articles, current and classical literature. Ph.D STUDENTS GRADUATED Christina M. (Munck) O'Hara, Purdue University program, "The role of ependymal cells in the remodeling of lesioned axolotl spinal cord", Degree: December, 1992 Current Ph.D. Student Deborah A. Sarria Scarring in axolotl spinal cord regeneration M.S STUDENTS GRADUATED Mary Jo Janey Davis, Purdue, topic: "Temporary myogenin gene knock-out in chicken embryos", Degree: December 1997 Lloyd Henry, IU School of Medicine, Medical Neurobiology Program. topic: Reepithelialization of injury-reactive ependymal cells. Degree: May 1998 Angela Corn, Purdue, topic: A tissue culture model system for Xenopus spinal cord regeneration. Degree May 2003 UNDERGRADUATE RESEARCH/CAPSTONE STUDENTS (39; 36 (Chronologically, 1987-present. In rank = ) Susan Robertson (1987-1989) Heather Schimele (1989-1990) Pamela Jones(1990-1991) Terry Spotts (1991-1992) Michelle Morris (1992-1993) Don Armenoff (1994) Connie Jeffries (1994) in rank)

Annette Howard (1994) Neda Davaloo (1996) Staci Miller (1997) Michael Bowling (1997) Traci Jones (1998) Carla Riley (1998) George Paleologos (1999) Debra Gonzales (1999) Louis Craig (1999 non-degree) Aaron Fairchild (2000, Univ. of Missouri, Rolla student, Daren Hemingway (2000) Pamelia Phelps (2000) Angela Corn (2000-2001) Stephanie Van Dyke (2001, Capstone paper) Ben Dugas (2001, Capstone) Melissa Squier (2001-2002) Jennifer Obertuch (2002) Amie Burns (2002-2003) Dori Smith (2002 Capstone) Lawanda Beck(2002-2003) Jerry Hege (2003-) Hai Nguyen (2003- Minority Res Scholar) Yennie Ma (2004). Elizabeth Tuttle Patterson (2004) Matthew Farmer (2005-) Josephine Harris (2005-2006)

transient)

Todd Philliips (2005 , Capstone paper) MaryAnn Steffen, 2006 Rachel Dickenson (2006-) Kelley Wilson (2008-2010) Nicholas McKeehan (2009) Kimberly Theeuwes (2009-2010) Lyss Woods (2009-2010)

Of the undergraduate research students that I have had contact with: Annette Howard Beasley and Louis Craig graduated medical school. Angela Corn graduated veterinary school. George Paleologos became a pharmaceutical company sales representative. Pamelia Phelps is working at Eli Lilly. Terry Spotts is a high school science teacher. Hai Nguyen is in the IU Schoolof Medicine. Matthew Farmer is getting a MS in herpetology Nicholas McKeehan is working at Einstein Medical College in NYC POST-DOCTORAL FELLOWS
Dr. Dr.

Kazuna Sato, 1999-present Rachel Karcavich, 2001-2004

Awards to Chernoff Lab students: Christina OHara, Ph.D. Purdue PRF Fellowship, 1990 Debra Bauerle, Ph.D. student received RIF Doctoral Fellowship, 1992 Terry Spotts, Departmental Teaching Assistant Award, 1992 Debra Bauerle, Ph.D. student: Travel Scholarship to attend Fourth International Symposium on Neural Regeneration. $500. Awarded by the EPVA (Eastern Region Paralyzed Veterans of America), 1993 Josephine Harris UROP, $1,300 supplies ($1,200 stipend to student), 2005-2006 Hai Nguyen, LSAMP summer fellowship 2004; SROP summer 2005, SROP summer 2006, UROP 2005: total $6,600 supply funds (plus stipends to student) Other Education-related activities: 2004 Summer Mentor. LSAMP (Louis Stokes Alliance for Minority Participation) 2003 Fall, 2004 Spring, Fall. Mentor for Diversity Research Scholars Program. Student: Hai Nguyen 2003-2004. Hosted Sabbatical Visiting Professor Won-Sun Kim from Sogang University, Seoul Korea, 1 year SERVICE ACTIVITIES Professional Service Reviewer Service Journals (in rank only: alphabetical order with dates and numbers: 34 total, 13 journals) Biochemical Pharmacology: 2004-1 article Canadian Journal of Zoology: 1994-1 article Current Neurovascular Research: 2003-1 article Developmental Biology: 2000-1 article; 1992-1 article Developmental Dynamics: 2007-1; 2006-1; 2004-1 article; 2002-3 articles; 1997-2 articles Development, Genes and Evolution: 1998-1 article Development, Growth and Differentiation: 1996-2 articles; 1995-1 article; 1993-2 articles; 1992: 3 articles Genes and Development: 1998-1 article; International Journal of Developmental Biology: 1999-1 article; 1997-1 article; 1993-1 article; 1992-1 article Journal of Experimental Zoology: 2004-1 article, 2005-1 article Journal of Neuroscience Research: 2005-2 articles Journal of Vacuum Science and Technology A: 2003-1 article; 1997-1 article; 1994: 2 articles; 1993: 2 articles Journal of Vacuum Science and Technology B: 2005-1 article

Journal of Zoology: 2005:1 Proceedings Indian National Academy of Science-Reviews: 2003-1 article Proceeding National Academy of Science (USA): 2001-1 article

Grant Reviews and Grant Panels (in rank only) Biotechnology and Biological Sciences Research Council (England, United Kingdom) Research grant application, Institute of Child Health: 2004-1 grant Eli Lilly External Contracts Review Committee, 1995 Health Research Board (Ireland, UK): 2004-1 grant NSF Grant Review Panel: Partnerships for Innovation, Industrial Panel, June 2002, 20 proposals NSF: 1992-1 grant Spinal Cord Research Foundation: 2000-1 grant USDA: 1999-1 review; 1996-1 grant NSF: 2005-2 Canada Foundation for Innovation: 2006 Expert reviewer, site visit IUPUI-RSFG panel: 2006 Consultancies. Hooks Foundation, advising for new science museum 2002 Offices Held Member, Awards Committee. Marcus Singer Society for Regeneration and Developmental Biology, Symposium Awards 1998-2004

Department of Biology Search and Screen Committees (in rank only) Chair, Regenerative Biology Search, 2000-2001 (hire: Teri Belecky-Adams) Member, Regenerative Biology Search 1999-2000 (no hire) Member, Regenerative Biology Search 1997-1998 (cancelled) Chair, Molecular Development Search, 1994-1995 (hire: Simon Rhodes) Chair, Cell Biology Search 1993-1994 (hire: Pamela Crowell) Chair, Two joint Biology/BME positions 2006, in progress Other (in rank only) Explore IUPUI Day volunteer 2004, 2003 Connect Tech exhibitor 2003 Graduate Student Recruitment (poster and college visits), 1993

School IACUC (SOS Institutional Animal Care and Use Committee): 2000-present School of Science Faculty Assembly President (and Chair of Steering Committee): 1999, 2000 School of Science Bulletin: 1998, 2000, 2001, 2003, 2005

University/IU System Medical Neurobiology Curriculum Revision Committee 2004 Director, Indiana University Center of Excellence in Regenerative Biology and Medicine: 2001present Future of IUPUI Committee: 1999 IUPUI Faculty Council: 1997, 1998 Laboratory demonstrations, high school groups, Office of Communications and Public Relations, 1995 Sigma Xi membership committee, 1993 New Faculty Orientation, 1993 Laboratory demonstrations for K-12 groups, Campus Tours Office, 1993

Community (in rank) Fund Raising Committee, Class of 1973 30th Reunion, The University of Chicago: 2002-2003 Scientist Apprentice Camp project leader (organizer: Gordon Fricke): 1994, 1998 and 2000 Girls Incorporated Operation S.M.A.R. T. (Science, Math And Relevant Technologies) Advisory Board, 1992-1995 Operation S.M.A.R.T summer camp, (for Girls Incorporated) 1992 Ask-a-Scientist series at the Indiana Childrens Museum, 1992 Admissions interviewer for The College of the University of Chicago: 1992-1997 Other Professional Activities Symposium Presentations and Invited Seminars (in Rank) International Meetings 9th International Conference on Limb Development and Regeneration. Talk titled: the analysis of the regeneration incompetent axolotl mutant short-toes. July 24-28, 2006 Awaji, Japan 1. 8th International Conference on Limb Development and Regeneration. Talk titled: Neural stem cell marker Musashi expression in Xenopus and axolotl limb. Dundee, Scotland. July 15-18, 2004.

2. Biocomplexity IV: Regenerative Biology and Medicine. International workshop Talk titled: Neural Stem Cell Properties and Amphibian Spinal Cord Regeneration. May 14-18, 2003 Indiana University Bloomington. 3. Regeneration In The 21st Century, Wistar Institute, Philadelphia. Talk titled: Ependymal Cells, A Key to Spinal Cord Regeneration. May 5-6, 1999 4. Metamorphosis and Regeneration: Keys to Tissue Restoration via Biological Remodeling, International Meeting in Indianapolis, Invited Symposium Speaker. Talk titled: The Role of Ependymal Cells in the Onset of Spinal Cord Regeneration,. Sponsored by ERATO-JST, Yoshizato MorphoMatrix Project, Hiroshima, Japan. Held in Indianapolis August 27-29, 1997 5. Symposium on Cell and Tissue Culture of Urodeles. Talk titled: Urodele neural cell culture. International Workshop on the Molecular Biology of Axolotls and Other Urodeles, Indianapolis, October 13-16, 1993

Regional and National

Chernoff EAG (2007) McKnight Brain Institute (U Florida) Regeneration Project I Symposium. Dec 13-15 Amelia Island Plantation, FL. Talk: The Ependymal Response in Urodele Spinal Cord. What is Different in Mammals? Chernoff EAG and Sato K (2007) Stem/progenitor cells in amphibian limb and spinal cord regeneration. Experimental Biology Meetings, Washington DC May 1, Invited talk Regenerative Biology and Medicine Minisymposium, Abstract 320 Chernoff EAG (2008) Wabash College, Dept of Biology, Crawfordsville IN. The ependymal response in urodele spinal cord. Oct. 2

1. AALAS District V Regional Meeting. Colloquium on non-mammalian research animals. Talk titled: Axolotl Husbandry from Eggs to Adults. Indianapolis, April 26, 2001 2. Banquet Address, 40th Midwest Regional Developmental Biology Meeting and Singer Symposium, Univ. of Illinois. Talk titled: How to Speak Ambystomatid. HumanSalamander Interactions in Captivity (a seminar on salamander behavior), May 25, 2000 3. Chernoff, E.A.G. (1999) Ependymal cells, calcium and spinal cord regeneration. 39th Annual Midwest Regional Developmental Biology Conference, Univ. IL 4. Marcus Singer Symposium on Regeneration and Developmental Biology, Annual Meeting, Indianapolis. Talk titled: Cutting the Cord: the Role of Ependymal Cells in Spinal Cord Regeneration, May 31, 1997 5. Mini-Symposium on Tissue Regeneration, American Society for Zoology national meeting, St. Louis.Urodele spinal cord regeneration. January 1995 6. Miami University of Ohio, November 1994, Department of Zoology, Scanned Probe Microscopy of Extracellular Matrix.

Local Chernoff EAG and Sato K. (2008) Axolotl Regeneration: Scarring in the spinal cord and the short-toes mutant limb. IUPUI Department of Biology Sept. 19.

Chernoff EAG (2006) Stem Cells in Amphibian Regeneration: Spinal Cord and Limb. IUPUI Dept of Biology September 22. Chernoff EAG (2006) Stem Cells in Amphibian Regeneration: Spinal Cord and Limb. Or: What the heck is dedifferentiation anyway? Developmental Biology Interest Group seminar. October 23.
1. Chernoff EAG (2004) Developing a Regenerative Medicine: Limb and Spinal Cord

Regeneration. LSAMP, IU School of Nursing. June 21, 2004 2. Chernoff EAG (2003) Seminar, IU School of Dentistry. Indiana Section of the American Association for Dental Research Seminar Series. Neural Stem Cell Properties and Amphibian Spinal Cord Regeneration. October 24, 2003. 3. Myogenin expression and somite differentiation in mouse and chick. (1996) Elanco (Animal Health Division of Eli Lilly and Company). Aug. 1996 4. All paths lead to collagen? Somite formation and development. (1996) IUPUI Dept Biol. June 25. Sabbatical work 5. Ependymal Cells and Spinal Cord Injury (1995) IU School of Med,, Dept of Anatomy Seminar, Jan. 26 6. Indiana University School of Medicine, Department of Microbiology and Immunology. The role of Ependymal Cells in Spinal Cord Regeneration. Fall 1992

Other Meetings Attended The Frontiers of the Biology of Amphibia. The International Symposium. Hiroshima, Japan. March 22-24, 1999. Invited guest, Yoshizato MorphoMatrix Project. Defense Sciences Research Council. Tissue Regenertion and Technology: Present Status and Directions for the Future. DARPA/DSRC. Chicago Nov 22, 1996

RESEARCH ACTIVITIES (in rank) RESEARCH SUPPORT (14 current or past external grants funded in rank) External-Present

1. "Problems of Amphibian Limb and Spinal Cord Regeneration", W.M. Keck Foundation. Director.: David Stocum, Co-P.I.: Ellen Chernoff (other Co-PIs: JoAnn Cameron, Teri Belecky-Adams, Mu Wang). 9/1/06-8/31/09. $1,600,000. (Chernoff portion $361,000) 2. Morton Cure Paralysis Fund. Proposal title: Ependymal cells and musashi: stem cells in spinal cord regeneration. Co-PIs: Ellen Chernoff, Kazuna Sato, Teri Belecky-Adams, Vladimir Uversky. First year: $69,996. Funded 3/26/08, Start 7/1/08

External-Past (line indicates those in rank) Discovering the Secrets of Regeneration: a Comparative Strategy Indiana 21st Century Research and Technology Fund. P.I.: David Stocum. Co-P.I.: Ellen Chernoff. (other co-PIs: A. Neff, A. Mescher, M. King, S. Rhodes, T. Belecky-Adams) Chernoff portion: $332,400 5/15/04-6/30/07 , $1,600,000. 1. DARPA SBIR II (Defense Advanced Research Projects Agency), subcontract through The Technical Basis, LLC, San Diego California. Subcontract P.I.: David L. Stocum. Co-PI Ellen Chernoff (other Co-PI: A. Neff). Total subcontract $200,000. Chernoff portion: $70,000 Nov. 2003-Dec 2004 2. Regenerative Biology and Medicine: A Search for Regeneration Stimulating Molecules in Xenopus laevis Spinal Cord, Renewal. Agency: Eli Lilly and Company. 8/1/01-5/14/04. P.I.: Dr. Ellen Chernoff. $162,000 3. Novel approaches for tissue and organ regeneration. Agency: Indiana 21st Century Research and Technology Fund. 1/1/01-12/31/02. Extended through 1/1/01-12/31/03 P.I.: Dr. David Stocum, Co-PI: Dr. Ellen Chernoff (other Co-PIs: A. Neff, A Mescher, M. King). $879,338 4. Matching funds for NSF award Agency: Indiana 21st Century Research and Technology Fund. P.I.: Dr. Ellen Chernoff., 2001-2003 $60,000 5. Partnerships for Innovation: A Center of Excellence in Regenerative Biology. Agency: National Science Foundation. P.I.: Dr. Ellen Chernoff. (Co-PIs: D. Stocum, A. Neff, A Mescher, S. Rhodes) 10/01/00-9/30/03. Extended through 3/31/04 $600,000 6. Regenerative Biology and Medicine: A Search for Regeneration-Stimulating Molecules in Xenopus laevis. Agency: Eli Lilly and Company. Duration: 5/18/99-5/31/01. P.I.: Dr. Ellen Chernoff. $138,563 7. NSF Research Equipment Grant (1998), Participant with Geology and School of Dentistry, , P.I. Gary Rosenberg, Geology. Total Funds $170,000; Chernoff $9,200 in Biology Dept. equipment. 8. Support for Midwest Regional Developmental Biology Conference. Society for Developmental Biology, 1997. P.I. S. Rhodes, Co-PI Chernoff $4,200 9. Temporary myogenin gene knock out in embryonic chick. Agency: Eli Lilly Corporation 9/1/96-12/30/97. PI: Chernoff $9,991 10. Sabbatical year support: somite development and myogenesis Elanco Poultry Science Agency: Eli Lilly and Company. (two summers plus one semester supported by Lilly) 19951996. PI Chernoff. $43,000

11. Scanning probe microscope studies of collagen, phase II Agency: Collagen Corporation via subcontract from Advanced Surface Microscopy, Inc. 6/25/94-6/24/95. PI: E. Chernoff. $2,400 12. Scanning probe microscope studies of collagen, phase I. Agency: Collagen Corporation via subcontract from Advanced Surface Microscopy, Inc. 1/19/94-4/30/94. PI: E. Chernoff. $1,800 13. International Workshop on the Molecular Biology of Axolotls and Other Urodeles Agency: Society for Developmental Biology. September 1993 for meeting in October 1993. PI: G. Malacinski, Co-PI: Chernoff. Pending
NSF URM; Intensive Undergraduate Research Experience in Biological Signaling at IUPUI, full proposal invited, Participant

Christopher and Dana Reeve Foundation, Proposal title: Dorsal-ventral patterning and stem cells in urodele spinal cord regeneration. Co-PIs: Ellen Chernoff and Kazuna Sato, request:$150,000, 2 years Submitted 6/15/07 PUBLICATIONS Full-length Publications Refereed Papers and Book Chapters -In my field the corresponding/senior author is usually either the first author or the last author. 1. Chernoff, E.A.G. and Overton, J. (1977) Scanning electron microscopy of chick epiblast on the vitelline membrane. Cell-substrate interactions. Develop. Biol. 57: 33-46, 1977. 2. Chernoff, E.A.G. and Overton, J. (1979) Organization of the migrating chick epiblast edge; attachment sites, cytoskeleton and early developmental changes. Develop. Biol. 72: 291307. 3. Overton, J., Meyer, R. and Chernoff, E.A.G. (1981) Epithelial cell-cell and cell-substrate contacts. In: Scanning Electron Microscopy/1981. SEM, Inc. Chicago, pp.297-305 (vol.II). 4. Chernoff, E.A.G. and Lash, J.W. (1981) Cell movement in somite devlopment in the chick. Inhibition of segmentation. Develop. Biol. 87: 212-219. 5. Chernoff, E.A.G. and Hilfer, S.R. (1982) Calcium dependence and contraction in somite formation. Tissue and Cell 14: 435-449. 6. Chernoff, E.A.G., Maresh, G.A. and Culp, L.A. (1983) Localization and characterization of a large, neurite-associated glycoconjugate. J. Cell Biol. 96: 661-668. 7. Maresh, G.A., Chernoff, E.A.G. and Culp, L.A. (1984) Heparan sulfate proteoglycans of human neuroblastoma cells: affinity fractionation on columns of platelet factor-4. Arch. Biochem. Biophys. 233: 428-437, 1984. 8. Chernoff, E.A.G. (1985) Cell movement and contraction in somite development. Scanning Electron Microscopy/1985. SEM, Inc., Chicago, pp.257-267.

9. Chernoff, E.A.G., (1986) Cell movement and contraction in somite development. In: "Studies in Embryogenesis", ed. G. C. Schoenwolf. SEM, Inc., Chicago, pp. 169-180. 10. Chernoff, E.A.G., (1988) The role of endogenous heparan sulfate proteoglycan in adhesion and neurite outgrowth from dorsal root ganglia. Tissue and Cell 20: 165-178. 11. Chernoff, E.A.G., (1989) Adhesion and fusion of the extraembryonic epiblast. Tissue and Cell 21: 735-746. 12. Chernoff, E.A.G., and Robertson, S. (1990) Epidermal growth factor and the onset of epithelial wound healing in embryonic epidermis. Tissue and Cell 22: 123-135. 13. Chernoff, E.A.G., Munck, C.M., Mendelsohn, L.G., and Egar, M.W., (1990) Primary culture of axolotl spinal cord ependymal cells. Tissue and Cell 22: 601-613. 14. O'Hara (nee Munck), C.M., M.W. Egar, and Chernoff, E.A.G., (1992) Reorganization of the ependyma during axolotl spinal cord regeneration: Changes in intermediate filament and fibronectin expression. Developmental Dynamics, 193: 103-115. 15. Chernoff, E.A.G. and Chernoff, D.A. (1992) AFM images of collagen fibers. Journal of Vacuum Science and Technology A, 10: 596-599. 16. Chernoff, E.A.G., Chernoff, D.A. and Kjoller, K. (1993) Contact and non-contact atomic force microscopy of type I collagen. Proceedings 51st Ann. Meeting of the Microscopy Society of America, ed.: G.W. Bailey and C.L. Rieder, San Francisco Press, pp. 518-519 17. O'Hara, C.M. and Chernoff, E.A.G. (1994) Growth factor modulation of injury-reactive ependymal cell proliferation and migration. Tissue and Cell, 26: 599-611 18. Kedishvili, N.Y., Gough, W.H., Chernoff, E.A.G., Hurley, T.D., Stone, C.L., Bowman, K.D., Popov, K.M., Bosron, W.F. and Li, T-K (1997) cDNA sequence and catalytic properties of a chick embryo alcohol dehydrogenase (ADH-F) that oxidizes retinol and 3,5hydroxysteroids. J. Biol. Chem. 272: 7494-7500. 19. Chernoff, E.A.G., Henry, L.C. and Spotts, T. (1998) An Ependymal Cell Culture System for the Study of Spinal Cord Regeneration. Wound Rep. Reg. 6: 435-444 20. Chernoff, E.A.G., OHara, C.M., Bauerle, D. and Bowling, M. (2000) Matrix Metalloproteinase Production in Regenerating Axolotl Spinal Cord. Wound Rep. Reg.8: 282-291 21. Chernoff,E.A.G., Clarke, D.O.,Wallace-Evers, J.L.,Hungate-Muegge, L.P. Smith, R.C. (2001) The Effects of Collagen Synthesis Inhibitory Drugs on Somitogenesis and Myogenin Expression In Cultured Chick and Mouse Embryos. Tissue and Cell, 33: 97110 22. Nye HLD, Cameron, J., Chernoff EAG and Stocum DL (2003) Extending the Table of Stages of Normal Development of the Axolotl: Limb Development. Devel Dyn 226:555-560 (Cover picture) 23. Showalter AD, Yaden BC, Chernoff EAG and Rhodes SJ (2003) Cloning and Analysis of Axolotl ISL2 and LHX2 LIM-Homeodomain Transcription Factors. Genesis (Developmental Genetics) 38:110-121. 24. Johnson CD, Narasimha Chary S, Chernoff EA, Zeng Q, Running MP, Crowell DN. (2005) Protein Geranylgeranyltransferase I Is Involved in Specific Aspects of Abscisic Acid and Auxin Signaling in Arabidopsis. Plant Physiol. 139:722-733. 25. Wilson J, Sato K, Chernoff EAG, Belecky-Adams T (2007) Expression Patterns of Chick Musashi-1 in the Developing Nervous System., Mech Of Dev, GeneExp 7:817-825

26. Sato K and Chernoff EAG (2007) The short-toes mutation of the Axolotl. Dev Growth Diff 49:469-478 27. Blazer-Yost, B.L., A. J. Banga, A. Amos, E. Chernoff, X. Lai, C. Li, S. Mitra and F.A. Witzmann. Effect of carbon nanoparticles on barrier function of renal epithelial cells. Nanotoxicology IN PRESS 8/4/10 28 Santosh, N; Windsor, L; Mahmoudi, B; Li, BB; Zhang, W; Chernoff, E; Rao, N; Stocum, D; Song, F.(2010) . Matrix Metalloproteinase Expression During Blastema Formation in Regeneration-Competent versus Regeneration-Deficient Amphibian Limbs Dev Dynam. IN PRESS 11/19/10 29.. Sato K, Corn A, Smith RC and Chernoff EAG (2007) Musashi Expression and Regeneration Competence in Xenopus laevis and Axolotl Spinal Cord. In Revision Invited or Contributed Review Articles, Peer-Reviewed 1. Chernoff, E.A.G., Stocum D.L. (1995) Developmental aspects of spinal cord and limb regeneration. Development, Growth and Differentiation., 37: 133-147 2. Chernoff, E.A.G. (1996) Spinal cord regeneration: a phenomenon unique to urodeles? International J. Develop. Biol. 40: 823-831. 3. Kedishvili, N.Y., Stone, C.L., Popov, K.M. and Chernoff, E.A.G. (1997) Role of alchohol dehydrogenases in steroid and retinoid metabolism. In: Molecular Biology of Carbonyl Metabolism. Edited by H. Weimer, D.W. Crab, T.G. Flynn. Plenum Press, NY. Adv. Exp. Med. and Biol. vol. 6, pp. 321-329. 4. Chernoff EAG, Sato K, Corn A and Karcavich RE (2002) Spinal Cord Regeneration: Intrinsic Properties and Emerging Mechanisms. Sem Cell Develop Biol 13:361-368 5. Nye HL, Cameron J, Chernoff EAG and Stocum DL. (2003) Regeneration of the Urodele Limb: A Review. Develop. Dyn. 226:280-294. 6. Chernoff EAG, Stocum DL, Nye HLD and Cameron JA (2003) Urodele Spinal Cord Regeneration and Related Processes. Devel Dyn 226:295-307. Popular Science Articles and Technical/Non-reviewed Articles 1. Chernoff, E.A.G. (1998) Nursing your tiger salamander through metamorphosis. Reptile Hobbyist #34 4 (4): 45-50 2. Chernoff, E.A.G. (1999) Acclimate your adult tiger salamander to life in captivity. Reptile Hobbyist #37, vol. 4 (7): 27-34 3. Chernoff, E.A.G. (1999) Taming your adult tiger salamander: use their behavior as a guide. Reptile and Amphibian Hobbyist #42, vol. 4 (12): 56-59 4. Chernoff, E.A.G. (2000) Where Does Tame Ambystomatid Salamander Behavior Come From? 5. What is normal behavior? A look at the literature. Reptile and Amphibian Hobbyist

#50, vol. 5 (8): 49-55 6. Chernoff, E.A.G. (2001) Tiger salamander behavior changes with growth and maturation. Reptile and Amphibian Hobbyist. #59, vol.6 (6): 49-54 7. Stocum DL, Nye HLD, Cameron JA and Chernoff EAG (2003) What we know about urodele (especially axolotl) early development: a review of gamete formation and fertilization. Axolotl News 30:4-11 (Publication of the Indiana University Axolotl Colony). print and web: http://www.indiana.edu/~axolotl/newsletter/currentissue.html. Issue 30.

Web Publications 1. Chernoff EAG (2002) Salt Solutions in Treating Salamander Diseases. Caudate Central Web site. http://www.caudata.org/caudatecentral/articles/salt.html Abstracts From Meeting Presentations (In Rank Only) 1. Chernoff, E.A.G., Chernoff, D.A. and Kjoller, K. (1992) Contact and non-contact AFM of type I collagen. Regional Scanning Probe Microscopy Users Meeting, Purdue University, West Lafayette, IN, December 18, 1992. Platform presentation 2. Chernoff, E.A.G., Chernoff, D.A. and Kjoller, K. (1992) Contact and non-contact atomic force microscopy of type I collagen. American Society for Cell Biology, Denver CO, November 1992, Molec. Biol. Cell, 3:227a. Poster presentation. 3. Chernoff, E.A.G., Chernoff, D.A., Kjoller, K. (1992) Contact and non-contact atomic force microscopy of type I collagen. Surface Biomaterials Symposium, October 1992, Minneapolis, MN, Platform Presentation 4. E.A.G. Chernoff, D.A. Chernoff, and K.J. Kjoller (1993) Contact and non-contact atomic force microscopy of collagen. Bulletin of the American Physical Society, 38: 119 5. E.A.G. Chernoff, D.A. Chernoff, and K.J. Kjoller (1993) Contact and non-contact atomic force microscopy of collagen. American Vacuum Society, p.162 6. Bauerle, D.J. and Chernoff, E.A.G. (1993) Matrix metalloproteinase production in spinal cord regeneration. Indiana Academy of Science, 109th Meeting Abstracts, p.20 7. Bauerle, D.J. and Chernoff, E.A.G. (1993) Matrix metalloproteinase production in spinal cord regeneration. Fourth International Symposium on Neural Regeneration, Asilomar, CA. 8. D.A. Chernoff and E.A.G. Chernoff (1993) Multimode atomic force microscopy of collagen. American Chemical Society National Meeting, POLY 0178. 9. Bauerle, D.J. and Chernoff, E.A.G. (1993) Matrix metalloproteinase production in spinal cord regeneration. International Workshop on the Molecular Biology of Axolotls and Other Urodeles, Indianapolis, IN. 10. Chernoff, E.A.G., Chernoff, D.A. and Kjoller, K. (1993) Contact and non-contact atomic force microscopy of type I collagen. 51st Ann. Meeting of the Microscopy Society of America

11. Chernoff. D.A. and Chernoff, E.A.G. (1994) Atomic force microscopy of collagen monomers, fibrils and fibrillar complexes. Materials Research Society, Fall Meeting, Dec. 1994, Denver, CO., p.599 12. Chernoff, E.A.G. (1994) Cutting the cord: spinal cord regeneration in urodele amphibians. Am. J. Zool. 34: 8AA. St. Louis, Jan. 1995. 13. Kedishvili, N., Chernoff, E. (1995) Identification of a novel alcohol dehydrogenase (ADH) in early chick embryo. Retinoid Society of America, Annual Meeting. 14. Chernoff, E.A.G., Chernoff, D.A. and Vogel, K. (1995) Proteoglycan/collagen interactions examined by atomic force microscopy. Eighth International Conference on Scanning Tunneling Microscopy/Spectroscopy (STM'95), Snowmass CO 15. Lee DL, Chernoff EAG and Smith RC (1996) Effects of collagen synthesis inhibitors on skeletal muscle development in vitro. Midwest Developmental Biology Meeting, March 1417, Chicago. 16. Wallace JL, Clarke DO, Hungate, LP, Chernoff EAG and Smith RC (1996) The effect of collagen synthesis inhibitors on myogenesis in vivo. Midwest Developmental Biology Meeting, March 14-17, Chicago 17. Chernoff EAG, Clarke DO, Hungate LP, Lee DL, Wallace JL and Smith RC (1996) The effects of collagen synthesis inhibitors on myogenesis in vitro and in vivo. 5th International Limb Development and Regeneration Conference. York, England, March 24-27. 18. Clarke DO, Hungate LP, Chernoff EAG, JL Wallace and Smith RC (1996) Inhibition of collagen synthesis in cultured mouse embryos: Effects on development and myogenin expression. Teratology Society Meeting, June 22-27, Keystone, CO. 19. J. Wallace Evers, E. Chernoff, D.Lee, D.O. Clarke, L. Hungate, R.C. Smith (1997) Midwest Developmental Biology Conference, May 1997, Indianapolis. Modulation of myogenin expression by collagen synthesis inhibitors. 20. J. Wallace Evers, E. Chernoff, D.Lee, D.O. Clarke, L. Hungate, R.C. Smith (1997) Keystone Conference on Molecular Control of Myogenesis. March 1997. Modulation of myogenin expression by collagen synthesis inhibitors. 21. M.J. Davis and E. Chernoff (1997) Development of an Antisense Oligonucleotide Delivery System. Midwest Developmental Biology Conference May 1997, Indiananpolis. 22. E. Chernoff, K Vogel, D. Chernoff (1997) International Digital Instruments Users Meeting (June, Santa Barbara, CA). Atomic Force Microscopy of Collagen/Small Proteoglycan Interactions. 23. Swartz, D.R., Chernoff, E. and Lim, S.S. (1997) Microtubules in cultured chick cardiac myocytes. Biophys. J. 72: Tu295 24. Henry, L.C. and Chernoff, E.A.G. (1998) An epithelial ependymal cell model system for spinal cord regeneration. 38th Annual Midwest Developmental Biology Conference, p. 10 25. Chernoff, E.A.G. (1999) Ependymal cells, calcium and spinal cord regeneration. 39th Annual Midwest Developmental Biology Conference, p.13 26. Nguyen, T., Sato, K., Neff, A., Chernoff, E., King, M., Mescher, A., Chalfant, C., Sankhavaram, P., Stocum, D. and Smith, R. (2000) Genes expressed during Xenopus limb and spinal cord regeneration using subtractive hybridization. 40th Annual Midwest Developmental Biology Conference, Poster presentation, p. 25

27. Chernoff, E.A.G. (2000) How to speak Ambystomatid. Human-salamander interactions in captivity. 40th Annual Midwest Developmental Biology Conference. May 25. After Banquet Address. Abstract P.20 28. Chernoff, E.A.G. (2000) Care and Behavior of terrestrial salamanders. AALAS Indiana Chapter, Eli Lilly and Company. Nov. 1 29. Chernoff, E.A.G. (2001) Axolotl Husbandry from Eggs to Adults. AALAS District V Regional Meeting, Indianapolis, April 26, 27 30. Chernoff, E.A.G., Sato, K., Smith R.C. (2001) Nrp-1 (Musashi) and Shh Expression in Ependymal Cells of Regenerating and Non-Regenerating Xenopus Spinal Cord. 41st Annual Meeting, American Society for Cell Biology, Washington, D.C. Dec.8-12. Poster. Molec. Biol. Cell 12: 366a 31. Karcavich, RE, Corn A., Squire, M, Sato, K and Chernoff, EA. (2002) Characterization of the cellular outgrowth resulting in spinal cord regeneration in the frog Xenopus laevis. 42nd Annual Midwest Regional Developmental Biology Meeting, University of Missouri, Columbia MO June 1-4 32. Sato, K, Smith, R.C. and Chernoff E.A.G. (2002) Nrp-1 (Musashi) and Shh Expression in Ependymal Cells of Regenerating and Non-Regenerating Xenopus Spinal Cord. 42nd Annual Midwest Regional Developmental Biology Meeting, University of Missouri, Columbia MO June 1-4 33. Sato K, Corn-Blevins A, Smith RC, Chernoff EAG. (2002) Nrp-1 (Musashi-1) Expression and regeneration competence in Xenopus laevis spinal cord in vivo and in vitro. Poster presentation at the Arthur M. Sackler Colloquium, National Academy of Sciences, Regenerative Medicine, Irvine, CA, October 18-20 34. Sato K, Corn A, Karcavich R, Chernoff EAG (2003) Neural Stem Cell Properties and Amphibian Spinal Cord Regeneration. Biocomplexity IV: Regenerative Biology and Medicine. International workshop at Indiana University Bloomington. May 14-18, 2003 ,p.10 35. Karcavich, RE, Corn, A, Chernoff EAG (2003) An in vitro method for studying spinal cord regeneration in Xenopus laevis. Biocomplexity IV: Regenerative Biology and Medicine. International workshop at Indiana University Bloomington. May 14-18, 2003 ,p.10 36. Sato K, Corn A, Karcavich R, Smith RC, Chernoff EAG. (2003) Neural stem cell properties and Xenopus spinal cord regeneration. Develop Biol 259:533-534. Society for Developmental Biology and International Society of Developmental Biologists Meeting. Boston, July 30-August 3, 2003. 37. Karcavich, RE, Sato, K and Chernoff EAG (2004) Xenopus brain an spinal cord culture as a source for neuronal stem (precursor) cells. Oral presentation. 44th Annual Midwest Regional Developmental Biology Meeting and Singer Symposium, June 5-8. Stowers Institute, Kansas City, MO, p. A5 38. Sato K, Karcavich, RE, Smith RC and Chernoff EAG (2004) Neural stem cell properties and Xenopus spinal cord regeneration. Poster. 44th Annual Midwest Regional Developmental Biology Meeting and Singer Symposium, June 5-8. Stowers Institute, Kansas City, MO. P.P16 (#33) 39. Sato, K, Karcavich RA, Smith RE and Chernoff EAG (2004) Neural stem cell marker, Musashi expression in Xenopus and axolotl limb. International Conference on Limb Development and Regeneration. Dundee Scotland, July 14-19 Oral Presentation

40. Wilson JM, Sato K, Chernoff E,Belecky-Adams TL (2005) Expression of RNA binding protein, MSI, in developing chick retina. Great Lakes Vison Research Confernce, Cincinnati OH 41. Sato, K, Smith RE and Chernoff EAG (2005) The possible existence of stem cells in Xenopus and axolotl limb regeneration. p. 37 Midwest Regional Developmental Biology, Chicago June 25-28 2005. Platform presentation 42. Wilson JM, Sato K, Chernoff E, Belecky-Adams TL (2006) Expression of the mRNA binding protein Msi-1 in developing chick retina. Great Lakes Vision Research Conference, Ann Arbor MI. 43. Sato K, Nguyen H, Chernoff EAG (2006) The analysis of the regeneration-incompetent axolotl mutant short toes. 9th International Conference on Limb Development and Regeneration, Awaji Japan, July 2006, Platform Presentation 44. Chernoff EAG and Sato K (2007) Stem/progenitor cells in amphibian limb and spinal cord regeneration. Experimental Biology Meetings, Washington DC May 1, Invited talk Regenerative Biology and Medicine Minisymposium, Abstract 320 45. F.A. Witzmann, A.D. Amos, E.A. Chernoff, S.M. Hong1, X.Y. Lai, H.N. Ringham, B.L. Blazer-Yost (2008).. Society of Toxicology 2008 meeting Seattle March 17-20 46. F.A. Witzmann, A.D. Amos, E.A. Chernoff, S.M. Hong1, X.Y. Lai, H.N. Ringham, B.L. Blazer-Yost (2008) Effect of carbon nanoparticle exposure on renal barrier epithelial cells in vitro Experimental Biology Meeting Apr 5-9 San Diego 47. Amos, A.D., F.A. Witzmann, E.A. Chernoff, D. Hong, X. Lai, H.N. Ringham and B.L. Blazer-Yost. Changes in 48.
cell function and protein expression of mouse renal principal cells, mpkCCD, after carbon nanoparticle exposure. FASEB. J. 22:942.2, 2008 D Sarria, HV Nguyen, MW Egar and EA Chernoff (2008) Meningeal organization and injury response in

amphibian spinal cord regeneration. Dev Biol 319: A122 49. K Sato and EA Chernoff (2008) Gene expression of axolotl limb regeneration mutant short toes. Dev Biol 319: 122-123 Internal grants SROP summers 2005 and 2006, Hai Nguyen, Mentor: Chernoff, $2,800 UROP 2005 Hai Nguyen: Mentor: Chernoff, $2000 LSAMP Summer $1,000. Mentor support for student project. Mentor: Chernoff, Summer 2004 Fluorescence Microscope for Embryology and Immunology teaching lab. IUPUI SOS . Technology Committee. Participant $24,000. Feb 1997 Purdue Research Foundation International Travel 1995 $800 for International Conference on Limb Development and Regeneration York England. PI-Chernoff 1996 Faculty Development Office Travel 5th International Neural Regeneration Symposium. $250 PI-Chernoff 1995 Axolotl Neuronal Cell Culture System IUPUI Grant-In-Aid for Research Total: $2,000, PIChernoff 1993 Grant-In-Aid for Travel: 4th International Neural Regeneration Meeting. $250, PI-Chernoff 1993 Urodele Meeting, Support for International Visitors. IU Bloomington, International Programs Office $500 PI-Chernoff 1993. A tissue culture system for the study of spinal cord regeneration IU, Grant-in-aid for research. $3,000 PI-Chernoff Fall 1992

CURRICULUM VITAE Guoli Dai, D.V.M., Ph.D. Assistant Professor Department of Biology Center for Regenerative Biology and Medicine Indiana University-Purdue University Indianapolis 723 W. Michigan Street, Indianapolis, IN 46202 Phone: (317) 278-3895 Fax: (317) 274-2846 Email: gdai@iupui.edu EDUCATION: 8/19878/1990: Ph.D., Molecular and Cellular Biology, College of Agricultural and Animal Sciences, Jilin University (New Name), P.R.China. 8/19848/1987: M.S., Molecular and Cellular Biology, Changchun Veterinary University, Changchun, P.R.China. 8/19808/1984: D.V.M., Changchun Veterinary University, Changchun, P.R.China POSITIONS: 8/2009Present: Assistant Professor, Department of Biology, Center for Regenerative Biology and Medicine, IUPUI, Indianapolis, IN. 9/20038/2009: Research Assistant Professor, Department of Pharmacology, Toxicology and Therapeutic; Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS. 10/20029/2003: Assistant Professor, Department of Internal Medicine, University of Texas Medical Branch, Galveston, TX. 8/19989/2002: Research Assistant Professor, Department of Molecular and Integrative Physiology, University of Kansas Medical Center. Associate Director, DNA Sequencing Core, Center for Reproductive Sciences, University of Kansas Medical Center. 4/19947/1998: Postdoctoral Fellow, Department of Molecular and Integrative Physiology, University of Kansas Medical Center. 8/19924/1994: Postdoctoral Fellow, Department of OB/GYN, Albert Einstein Medical Center, Philadelphia, PA. 8/1990 8/1992: Lecturer, College of Agricultural and Animal Sciences, Jilin University, P.R.China. RESEARCH GRANT: Ongoing Grant Support: 1RO1DK075968-01A2 (Dai): NIDDK
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04/01/200903/31/2014 $1,500,000 (total cost)

Nrf2, Hepatocyte Proliferation, and Liver Regeneration The goal of the project is to investigate the molecular mechanisms underlying transcription factor Nrf2regulated hepatocyte proliferation and liver regeneration. Role: Principal Investigator Grant Applications Reviewed by NIH or in Development: 1RO1DK084028-01 NIDDK Prolactin Receptor Signaling: Hepatocyte Proliferation During Pregnancy and Liver Regeneration The project focuses on investigating the role of hepatic prolactin receptor signaling system in regulating hepatocyte proliferation in response to pregnancy and liver injury. Role: Principal Investigator Status: First submission in October 2008. Reviewed by Hepatobiliary Pathophysiology Study Section and Unscored. In revision for resubmission. NIH RO1 Prolactin Receptor/MASH-1 Pathway: Modulation of Hepatic Stellate Cells The goal of the project is to determine the role of prolactin receptor/MASH-1 pathway in regulating the proliferation and differentiation of hepatic stellate cells during pregnancy and liver regeneration. Role: Principal Investigator Status: In development. Completed Grants: COBRE First Award (Dai) 07/01/2006 02/30/2009 Nrf2-ARE Pathway: Discovery of Novel Chemopreventive Compounds $225,000 (total cost) The goal of the project is to discover and identify novel chemopreventive compounds, which specifically activate Nrf2/ARE pathway, using a cell-based high throughput screening strategy. Role: Principal Investigator K-INBRE (Dai) 07/01/2005 06/30/2006 PXR Activation and Liver Regeneration $52,500 (total cost) The goal of the project was to evaluate the role of PXR in partial hepatectomy-induced liver regeneration. Role: Principal Investigator K-INBRE (Dai) 01/01/2005 06/30/2005 RXR-mediated Pathways Controlling Xenobiotics-induced Hepatocyte Proliferation $52,500 (totoal cost) The goal of this proposal was to elucidate the molecular mechanisms underlying RXRalpha-mediated hepatocyte proliferation. Role: Principal Investigator 5 RO3 HD37678 (Dai) 02/01/2000 01/31/2002 NICHD $150,000 (total cost) Intraplacental Pathway Modulating Trophoblast Cells The goal of this project was to define a pathway mediated by a placental hormone and its receptor controlling placental development. Role: Principal Investigator Andrew W. Mellon Reproductive Biology Grant Foundation (Dai)
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04/01/2000 3/30/2003

Maternal Adaptations to Implantation $420,000 (direct cost) The goal of this project was to investigate the roles of decidual signals in the maternal adaptations to implantation. Role: Junior Investigator PUBLICATIONS (Journal Articles): 1. G. Dai, G Xiong and Y Zhao. 1988. Analysis of protein, RNA fragments of recombinant virus C731 and preliminary determination of its immunogenicity. Bulletin of Changchun Veterinary University, 8:409-414. 2. G. Dai, Y Zhao and Y Shi. 1991. Studies on Construction of a Non- Pathogenic Adenovirus Vector in Genetic Engineering. Bulletin of Changchun Veterinary University, 11:317-324. 3. G. Dai and Y. Zhang. 1992. Studies on Expression of Pro-UK cDNA in SV40-COS System. Bulletin of Changchun University of Agricultural and Animal Sciences,12:220-227. 4. SCM Kwok, D Yang, G Dai, MJ Soares, S Chen and JP McMurtry. 1993. Molecular Cloning and Sequence Analysis of Two Porcine Seminal Proteins, PSP-I and PSP-II: New Members of the Spermadhesin Family. DNA and Cell Biology, 12:605-610. 5. SCM Kwok, G Dai and JP McMurtry. 1994. Molecular Cloning and Sequence Analysis of the cDNA Encoding Porcine Acrosin Inhibitor. DNA and Cell Biology, 13:389-394. 6. G Dai, SP Smeekens, DF Steiner, JP McMurtry and SCM Kwok.1995. Characterization of Multiple Prohormone Convertase PC1/3 Transcripts in Porcine Ovary. Biochimica et Biophysica Acta, 1264:1-6. 7. CB Cohick, G Dai, L Xu, S Deb, T Kamei, G Levan, C Szpirer, SCM Kwok and MJ Soares. 1996. Placental Lactogen-I Variant Utilizes the Prolactin Receptor Signaling Pathway. Molecular and Cellular Endocrinology, 116:49-58. 8. MJ Soares, BM Chapman, CB Cohick, CA Rasmussen, G Dai, T Kamei and KE Orwig. 1996. Differentiation of Trophoblast Endocrine Cells. Placenta,17:277-289. 9. G Dai, B Liu, C Szpirer, G Levan, SCM Kwok and MJ Soares. 1996. Prolactin-like Prolactin C Variant: Complementary DNA, Unique Six Exon Gene Structure, and Trophoblast Cell-Specific Expression. Endocrinology,137:5009-5019. 10. MJ Soares, G Dai, CB Cohick, H Mueller, and KE Orwig. 1996. The Rodent Placental Prolactin and Pregnancy. In: Endocrinology of Pregnancy, F.W. Bazer(ed), Humana Press Inc., New Jersey. 11. G Dai, W Imagawa, B Liu, C Szpirer, G Levan, SCM Kwok and MJ Soares. 1996. Rcho-1 Trophoblast Cell Placental Lactogens: Complementary DNA, Heterologous Expression, and Biological Activities. Endocrinology,137:5020-5027. 12. KE Orwig, G Dai, CA Rasmussen and MJ Soares. 1997. Decidual/Trophoblast Prolactin-related Protein: Characterization of Gene Structure and Cell-specific Expression. Endocrinology,138:2491-2500. 13. MJ Soares, H Muller, KE Orwig, TJ Peter, and G Dai. 1997. The Uteroplacental Prolactin Family and Pregnancy. Biology of Reproduction, 58:273-284. 14. G Dai, B Liu, BM Chapman and MJ Soares. 1998. A New Member of the Mouse Prolactin (PRL)-like Protein-C Subfamily, PRL-like Protein-C : Structure and Expression. Endocrinology,139(12): 5157-5163. 15. KE Orwig, MW Wolfe, CB Cohick, G Dai, TJ Peters and MJ Soares. 1998. Trophoblast-specific Regulation of Endocrine-related Genes. Trophoblast Research, 11:65-85.
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16. H Mueller, G Dai, and MJ Soares. 1998. Placental Lactogen-I (PL-I) Target Tissues Identified With an Alkaline Phosphatase-PL-I Fusion Protein. The Journal of Histochemistry & Cytochemistry, 46(6): 737-743. 17. G Dai, BM Chapman, D Wang, RA White, and MJ Soares. 1999. Prolactin-like Protein-A Gene Structure and Chromosomal Mapping. Mammalian Genome, 10: 78-80. 18. H Muller, B Liu, BA Croy, JR Head, JS Hunt, G Dai, and MJ Soares. 1999. Uterine Natural Killer Cells Are Targets For A Trophoblast Cell-Specific Cytokine, Prolactin-Like Protein-A. Endocrinology, 140: 4032-4039. 19. G Dai, MW Wolf, and MJ Soares. 1999. Distinct Regulatory Regions from the Prolactin-like Protein-C Variant Promoter Direct Trophoblast Giant Cell versus Spongiotrophoblast Cell-specific Expression. Endocrinology, 140: 4691-4698. 20. RH Renegar, CR Owens, G Dai, JP McMurtry, and SCM Kwok. 2000. Expression and localization of prohormone convertase 1/3 (SPC3) in porcine ovary. Mol Reprod Dev. 57(4):361-5. 21. N Sahgal, GT Knipp, B Liu, BM Chapman, G Dai, and MJ Soares. 2000. Identification of Two New Nonclassical Members of the Rat Prolactin Family. Journal of Molecular Endocrinology, 24: 95-105. 22. G Dai, D Wang, B Liu, J W Kasik, H Muller, RA White, GS Hummel, and MJ Soares. 2000. Three Novel Paralogs of the Rodent Prolactin Gene Family. Journal of Endocrinology, 166:63-75. 23. D Wang, R Ishimura, DS Walia, H Muller, G Dai, JS Hunt, N A Lee, JJ Lee, and MJ Soares. 2000. Eosinophils Are Targets For A Novel Uteroplacental Heparin-Binding Cytokine Decidual / Trophoblast Prolactin-related Protein. Journal of Endocrinology, 166:1-15. 24. T Kamei, S Jones, B Chapman, K MCGonigle, G Dai, and M Soares. 2000. The Phosphatidylinositol 3kinase/Akt Signaling Pathway Modulates the Endocrine Differentiation of Trophoblast Cells. Mol. Endocrinol, 6(7):1469-81. 25. G Dai, L Lu, S Tang, N Sahgal, and M Soares. 2002. Prolactin Family Miniarray: A Tool for Evaluating Uteroplacental-trophoblast Endocrine Cell Phenotypes. Reproduction, 24(6):755-65. 26. D Wiemers, L Shao, R Ain, G Dai, and M Soares. 2003. The Mouse Prolactin Gene Family Locus. Endocrinology, 44(1):313-25. 27. S Lee, G Dai, Z Hu, X Wang, J Du, and W Mitch. 2004. Regulation of Muscle Protein Degradation: Coordinated Control of Apoptotic and Ubiquitin-proteasome Systems by Phosphatidylinositol 3 Kinase. J. Am. Soc. Nephrol., 15(6):1537-45. (Equal contribution for the first and second authors) 28. R Ain, G Dai, J Dunmore, A Godwin, and M Soares. 2004. A Prolactin Pamily Paralog Regulates Reproductive Adaptations to A Physiological Stressor. Proc. Natl. Acad. Sci. U S A., 23;101(47):16543-8. 29. G Dai and YJ Wan. 2005. Animal Models of Xenobiotic Receptors. Current Drug Metabolism, 6(4):341-355. 30. V Rider, T Potapova, G Dai, and MJ Soares. 2005. Stimulation of A Rat Uterine Stromal Cell Line in Culture Reveals A Molecular Switch for Endocrine-dependent Differentiation. J. Endocrinology, 184(1):119-27. 31. G Dai, N Chou, L He, MA Gyamfi, JA Mendy, AL Slitt, CD Klaassen, and YJ Wan. 2005. Retinoid X Receptor Alpha Regulates the Expression of Glutathione S-transferase Genes and Modulates Acetaminophenglutathione Conjugation in Mouse Liver. Molecular Pharmacology, 68(6):1590-1596. 32. K Wang, AJ Mendy, G Dai, HR Luo, L He, and YJ Wan. 2006. Retinoids Activate the RXR/SXR-mediated Pathway and Induce the Endogenous CYP3A4 Activity in Huh7 Human Hepatoma Cells. Toxicological Sciences, 92(1):51-60.
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33. MA Gyamfi, MG Kocsis, L He, G Dai, AJ Mendy, YJ Wan. 2006. The Role of Retinoid X Receptor in Regulating Alcohol Metabolism. J. Pharmacol. Exp. Ther., 319(1):360-368. 34. G Dai, N Chou, L He, and YJ Wan. 2006. Acetaminophen Metabolism Does Not Contribute to Gender Difference in Its Hepatotoxicity in Mouse. Toxicological Sciences, 92(1):33-41. 35. SM Alam, T Konno, G Dai, L LU, D Wang, JH Dunmore, AR Godwin, MJ Soares. 2007. A Uterine Decidual Cell Cytokine Ensures Pregnancy-dependent Adaptations to A Physiological Stressor. Development, 134(2):407-15. 36. J Bustamante, G Dai, and MJ Soares. 2008. Pregnancy and Lactation Modulate Maternal Splenic Growth and Development of the Erythroid Lineage in the Rat and Mouse. Reproduction, Fertility and Development, 20(2):303-310 37. G Dai, L He, P Bu, and YJ Wan. 2008. Pregnane X Receptor Is Required for Normal Progression of Liver Regeneration. Hepatology, 47(4):1276-1287 38. Bustamante JJ, Copple BL, Soares MJ, Dai G. 2010. Gene profiling of maternal hepatic adaptations to pregnancy. Liver Int. 30(3):406-415 Manuscripts reviewed and in final revision: 39. G Dai, JJ Bustamante, and MJ Soares. Maternal Hepatic Growth Response to Pregnancy in the Mouse. MEETING ABSTRACTS:
1. SCM Kwok, D Yang, G Dai, MJ Soares, S Chen, and JP McMurtry. 1993. Molecular Cloning and Sequence

Analysis of Two Porcine Seminal Proteins, PSP-I and PSP- II. Biology of Reproduction, 48(suppl.1):108. 2. CB Cohick, G Dai, L Xu, S Deb, T Kamei, SCM Kwok and MJ Soares. 1995. Heterologous Expression and Characterization of Placental Lactogen-I Variant. 77th Annual Meeting of the Endocrine Society. Programs and Abstracts, Abstracts#P3-659. 3. MJ Soares, BM Chapman, CA Rasmussen, G Dai, T Kamei and KE Orwig. 1996. Differentiation of Trophoblast Endocrine Cells, The Maternal-Placental-Fetal Dialoque. International Workshop Sponsered By The Science and Technology Agency of Japan, Maui, Hawaii. 4. G. Dai and M.J. Soares. 1996. Prolactin-like Protein-C Variant: Complementary DNA, Unique Six Exon Gene Structure, and Expression. 10th International Congress of Endocrinology, Program & Abstracts, Volume I, Abstract# P1-717, P314. 5. Orwig, G. Dai and M.J. Soares. 1996. Decidual Prolactin Related Protein Gene Structure Is Unique Among Prolactin Family Members. 10th International Congress of Endocrinology, Program & Abstracts, Volume I, Abstract# P1-714, P313. 6. G Dai and M.J. Soares. 1996. Rcho-1 Trophoblast Cell Placental Lactogens: Complementary DNAs, Heterologous Expression, and Biological Activities. Biology of Reproduction, 54(suppl.1):123. 7. MJ Soares, BM Chapman, TJ Peters, KE Orwig, T Kamei, CB Cohick and G Dai. 1996. Trophoblast Cell Differentiation: Acquisition of Endocrine Activities. 13th Annual Rochester Trophoblast Conference, Banff, Canada.

8. H Mueller, G Dai, and MJ Soares. 1997. Generation and characterization of a placental lactogen-I (PL-I)Alkaline Phosphatase Fusion Protein for the Identification of PL-I Target Tissues. 79th Annual Meeting of Endocrine Society, Program & Abstracts, P2-437, 394. 9. G Dai, MW Wolf, and MJ Soares. 1997. Distinct Regulatory Regions from the Prolactin-like Protein-C Variant Promoter Direct Trophoblast Giant Cell versus Spongiotrophoblast Cell-specific Expression. 79th Annual Meeting of Endocrine Society, Program & Abstracts, P2-438, 394. 10. G Dai, T Takahashi, B Liu, BM Chapman, H Muller and MJ Soares. 1998. Heterologous Expression and Identification of Target tissues for Prolactin-like Protein-C (PLP-C) and PLP-C Variant (PLP-Cv). 80th Annual Meeting of the Endocrine Society, New Orleans, LA, Abstract P3-460. 11. H Muller, G Dai, BA Croy, JR Head, B Liu and MJ Soares. 1998. Pregnancy-associated Naturai Killer Cells Are regulated by a Trophoblast-specific Cytokine. 80th Annual Meeting of the Endocrine Society, New Orleans, LA, Abstract P3-498. 12. G Dai, B Liu, BM Chapman, KE Orwig, D Wang and MJ Soares. 1998. A New Mouse Member of the Prolactin-like Protein-C Subfamily: Structure and Expression. 80th Annual Meeting of the Endocrine Society, New Orleans, LA, Abstract P3-462. 13. G. Dai, T. Takahashi, B. Liu, and M.J. Soares. 1999. Identification of A Potential Intraplacental Regulatory Pathway. International Workshop on Embryogenesis and Implantation. Hawaii,Abstracts #P31, Program and Abstract Book, 105. 14. G Dai, D Wang, B Liu, J W Kasik, H Muller, RA White, GS Hummel and MJ Soares. 2000. Three Novel Paralogs of the Rodent Prolactin Gene Family. Annual meeting of Society of Biology of Reproduction. Oral presentation. Biology of Reproduction, 62(suppl.1):256. 15. N Sahgal, S Tang, L Lu, G Dai, MJ Soares. 2001. Placental Responses to Fetal Death: Depletion of Glycogen Cells and Upregulation of Insulin-like Growth Factor Binding Protein-2 and Nutrient Transport Protein Messenger RNAs. 83rd Annual Meeting of the Endocrine Society, Denver, Colorado. Abstract P3-2. 16. T Kamei, SR Jones, BM Chapman, JM Beal, S Tang, L Lu, G Dai, and MJ Soares.2001. Activation of the Phosphatidylinositol 3-Kinase/AKT Signaling Pathway During Trophoblast Cell Differentiation. 83rd Annual Meeting of the Endocrine Society, Denver, Colorado. Abstract P3-22. 17. G Dai, L Lu, S Tang, N Sahgal, MJ Soares. 2001. Evaluation of Placental/Trophoblast Cell Phenotypes with A Prolactin Family DNA Miniarray. 83rd Annual Meeting of the Endocrine Society, Denver, Colorado. Abstract P3-30. 18. G Dai, N Chou, L He, MA Gyamfi, JA Mendy, and YJ Wan. 2005. Retinoid X Receptor Alpha Regulates the Expression of Glutathione S-transferase Genes and Modulates Acetaminophen-glutathione Conjugation in Mouse Liver. Society of Toxicology, Central State Chapter meeting, 2004. 19. G Dai, N Chou, L He, and YJ Wan. 2006. Gender Difference of Acetaminophen-induced Hepatotoxicity in Mouse. Society of Toxicology, Central State Chapter meeting, 2004, Best poster award. 20. G Dai, N Chou, L He, and YJ Wan. 2006. Acetaminophen Metabolism Does Not Contribute to Gender Difference in Its Hepatotoxicity in Mouse. Society of Toxicology, New Orleans, 2005, Oral presentation. 21. G Dai, L He, P Bu, and YJ Wan. 2007. Pregnane X Receptor Is Required for Normal Progression of Liver Regeneration. Society of Toxicology Central State Chapter Meeting, Iowa. MEMBERSHIPS OF PROFESSIONAL ORGANIZATIONS:
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American Association for the Advancement of Science (AAAS) Society of Toxicology (SOT) American Diabetes Association (ADA) JOURNAL ad hoc REVIEWER Endocrinology Molecular Pharmacology Journal of Pharmacology and Experimental Therapeutics Placenta COMMITTEE Graduate Committee (Department of Biology) The Cellular and Developmental Biology Qualifying Exam Committee (Department of Biology) INVITED TALK Maternal Hepatic Adaptations to Pregnancy, 12/1/2009, Department of Physiology, Medical School, Indiana University, Indianapolis, IN Prolactin Receptor Signaling: Hepatocyte Proliferation During Pregnancy and Liver Regeneration, 3/30/2010, Biocomplexity Institute, Indiana University, Bloomington

Andrew R. Kusmierczyk Assistant Professor Department of Biology Indiana University-Purdue University Indianapolis Indianapolis, IN akusmier@iupui.edu Education 1993 - 1997 1997 - 2003 2003 2003 - 2009 B.Sc (Honors, First Class) - Biochemistry Queens University; Kingston, ON, Canada PhD - Biology Brown University; Providence, RI Postdoctoral Associate - Brown University (Providence, RI) Department of Molecular Biology, Cell Biology, and Biochemistry Postdoctoral Fellow - Yale University (New Haven, CT) Department of Molecular Biophysics and Biochemistry

Professional Appointments 2009 - 2010 Associate Research Scientist - Yale University (New Haven, CT) Department of Molecular Biophysics and Biochemistry 2010 - present Assistant Professor - IUPUI (Indianapolis, IN) Department of Biology Awards and Honors 1993 - 1997 1993 - 1997 1996 2004 - 2005 2004 - 2007 2005 - 2008 Lilias Kathleen Dolan Entrance Scholarship in Biochemistry Queens University; Kingston, ON, Canada Canada Scholarship in Science and Technology Ministry of Industry; Government of Canada John D. Schultz Science Student Scholarship Heart and Stroke Foundation of Ontario James Hudson Brown and Alexander B. Coxe Postdoctoral Fellowship in the Medical Sciences Yale University ($ 36,000) Terry Fox Foundation Postdoctoral Fellowship in Biomedical Sciences National Cancer Institute of Canada ($ 38,000 per annum) [application ranked 1st overall; award declined in order to accept CIHR award] CIHR Postdoctoral Fellowship Canadian Institutes of Health Research ($ 50,000 per annum) [awarded in 2004; deferred start date until 2005]

Publications 1. Kusmierczyk, A.R., and Martin, J. (2000) High salt-induced conversion of Escherichia coli GroEL into a fully functional thermophilic chaperonin. J. Biol. Chem. 275, 33504-33511.

2. Kusmierczyk, A.R., and Martin, J. (2001) Chaperonins keeping a lid on folding proteins. FEBS Lett. 505, 343-347. (Review) 3. Kusmierczyk, A.R., and Martin, J. (2001) Assembly of chaperonin complexes. Mol. Biotechnol. 19, 141-152. (Review) 4. Kusmierczyk, A.R., and Martin, J. (2003) Nucleotide-dependant protein folding in the group II chaperonin from the mesophilic archaeon Methanococcus maripaludis. Biochem. J. 371, 669-673. 5. Kusmierczyk, A.R., and Martin, J. (2003) Nested cooperativity and salt dependance of the ATPase activity of the archaeal chaperonin Mm-cpn. FEBS Lett. 547, 201-204. 6. Hill, B.C., Becker, L., Anand, V., Kusmierczyk, A., Marcovina, S.M., Rahman, M.N., Gabel, B.R., Jia, Z., and Koschinsky, M.L. (2003) A role for apolipoprotein(a) in protection of the low-density lipoprotein component of lipoprotein(a) from copper-mediated oxidation. Arch. Biochem. Biophys. 412, 186-195. 7. Li, X., Kusmierczyk, A.R., Wong, P., Emili, A., and Hochstrasser, M. (2007) beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint. EMBO J. 26, 2339-2349. 8. Kusmierczyk, A.R., Kunjappu, M.J., Funakoshi, M., and Hochstrasser, M. (2008) A multimeric assembly factor controls formation of alternative 20S proteasomes. Nat. Struct. Mol. Biol. 15, 237244. Featured on the cover and reviewed in: Rina Rosenzweig, R., and Glickman, M.H. (2008) Forging a proteasome -ring with dedicated proteasome chaperones. Nat. Struct. Mol. Biol. 15, 218 - 220 (2008) 9. Kusmierczyk, A.R., and Hochstrasser, M. (2008) Some assembly required: dedicated chaperones in eukaryotic proteasome biogenesis. Biol. Chem. 389, 1143-1151. (Review) 10. Hochstrasser, M., Deng, M., Kusmierczyk, A.R., Li, X., Kreft, S.G., Ravid, T., Funakoshi, M., Kunjappu, M., and Xie, Y. (2008) Molecular Genetics of the Ubiquitin-Proteasome System: Lessons from Yeast. Ernst Schering Found. Symp. Proc. (1), 41-66. 11. Kusmierczyk, A.R., Kunjappu, M.J., Kim, R.Y., and Hochstrasser, M. A Conserved 20S Proteasome Assembly Factor Requires a C-terminal HbYX Motif for Proteasomal Precursor Binding (in review at Nat. Struct. Mol. Biol.). Invited Lectures 2009 2009 2009 2010 Carnegie Mellon University (Pittsburgh, PA), Department of Biological Sciences University of Nebraska-Lincoln (Lincoln, NE), Department of Biochemistry SUNY Upstate Medical University (Syracuse, NY), Department of Biochemistry Indiana University-Purdue University Indianapolis (Indianapolis, IN) Department of Biology

Conferences and Presentations Oral Presentations: 2008 FASEB Summer Research Conference: Ubiquitin and Cellular Regulation Hochstrasser, M., and Kusmierczyk A.R. The roads not taken: Proteasome assembly and misassembly in vivo Poster Presentations: 2001 Canadian Society of Biochemistry, Molecular and Cellular Biology, Winternational 2001 Kusmierczyk, A.R., and Martin, J. Communication breakdown? Insights from two intermediate domain mutants of GroEL. Biochem. Cell. Biol. 79, 643. 2002 Gordon Research Conference on Protein Folding Dynamics

2002 2005 2007 2007

2008

2008 2009

Kusmierczyk, A.R., and Martin, J. Chaperonin from the mesophilic archaeon Methanococcus maripaludis Cold Spring Harbor Meeting: Molecular Chaperones and the Heat Shock Response Kusmierczyk, A.R., and Martin, J. Chaperonin from the mesophilic archaeon Methanococcus maripaludis Cold Spring Harbor Meeting: The Ubiquitin Family Kusmierczyk, A.R., and Hochstrasser, M. A Trans-vivo approach to identify proteasome subunit interactions during 20S proteasome assembly Cold Spring Harbor Meeting: The Ubiquitin Family Kusmierczyk, A.R., and Hochstrasser, M. Subunit interactions during 20S proteasome assembly EMBO Conference: Ubiquitin and Ubiquitin-like Modifiers in Cellular Regulation Kusmierczyk, A.R., and Hochstrasser, M. Short-lived Assembly Imaging Label (SAIL): A novel in vivo assay for protein complex assembly reveals aggregation-prone defects in proteasome assembly Cold Spring Harbor Meeting: Molecular Chaperones and Stress Responses Kusmierczyk, A.R., Kunjappu, M., Funakoshi, M., and Hochstrasser, M. A Proteasome specific chaperone regulates assembly of alternative proteasomes - Implications for a conserved stress response FASEB Summer Research Conference: Ubiquitin and Cellular Regulation Kusmierczyk, A.R., and Hochstrasser, M. Visualizing proteasome assembly events in vivo Cold Spring Harbor Meeting: The Ubiquitin Family Kusmierczyk, A.R., Kim, R., Kunjappu, M.J., Funakoshi, M., and Hochstrasser, M. The role of assembly factors in 20S proteasome biogenesis

Current Trainees Graduate 2010 - present Lindsay Hammack Teaching Experience 2000 2001 Teaching assistant BI127 Advanced Biochemistry Teaching assistant BI028 Introductory Biochemistry MS

Professional Activities 2001 2007 - 2008 2008 - 2009 Admissions Committee Member - Brown University Department of Molecular Biology, Cell Biology, and Biochemistry Organizer - Yeast at Yale Organized monthly seminar series for yeast-oriented research labs at Yale Connecticut State Science Fair Judge - Senior High School Category

CURRICULUM VITAE - Norman Douglas Lees

Department of Biology Indiana University Purdue University Indianapolis Indianapolis, IN 46202 Telephone: 317-274-0588 Fax: 317-274-2846 e-mail: nlees@iupui.edu

Personal: Birth: September 16, 1945 Marital Status: Married; Stephanie Louise (Lofton) Children: Matthew Thomas, 1981; Megan Emily, 1984; Ethan Douglas, 1986; Mitchell Jordan, 1992 Education: Ph.D., Biological Sciences, Northwestern University, 1973 A.B., Biology, Providence College, 1967 Professional Experience: Professor of Biology and Special Assistant to the Dean, 2010Emeritus Faculty of University College, 2010Adjunct Professor of Biomedical Engineering, 2002-present Professor and Chairman of Biology, 1997-2010 Founding Faculty of University College, 1997-present Adjunct Associate Professor of Education, 1995-1997 Associate Professor and Chairman of Biology, 1992-1997 Associate Professor of Biology, IUPUI, 1980-1992 Assistant Professor of Biology, IUPUI, 1973-1980 Teaching Assistant, Northwestern University, 1967-1973 Professional Memberships past and present: American Society for Microbiology (ASM) Indiana Branch ASM Sigma Xi (IU Medical Center Chapter) Indiana Academy of Science American Association for Higher Education Honors, Awards and Special Appointments: American Men and Women of Science Johnson and Johnson Focused Giving Symposium Invited Presenter, 1995

University of Qatar Commemorative Medal, 1998 Advisory Board of the National Chairpersons Conference (sponsored by Kansas State University), 2002-present Full Member, Indiana University Graduate Faculty, 2003 Biotechnology Advisory Board, 2004-6 Advisory Board, The Department Chair, Jossey-Bass (Wiley) Pub., 2007-present Teaching: Courses taught: BIOL 420/520, Cell Biology BIOL 421/521, Cell Biology Lab BIOL N105, Botany (lab and recitation only) BIOL 104, Principles of Biology BIOL K101-K103, Concepts of Biology, modular format BIOL K203, Concepts of Biology IV BIOL K101, Concepts of Biology I - Plants BIOL N107, Zoology BIOL K323, Genetics Lab BIOL 697/540, Topics in Biotechnology, selected lectures BIOL 530, Introductory Virology BIOL K356, Microbiology BIOL 532, Topics in Bacteriology BIOL K357, Microbiology Lab New courses developed: BIOL K356, Microbiology BIOL K357, Microbiology Lab BIOL 530, Introductory Virology BIOL 532, Topics in Bacteriology Degree programs developed: Pre-Professional Master of Science, 1993-present. Associate of Science in Applied Biotechnologies (ASAB), ICHE approval 6/04, launched 8/04 Bachelor of Science in Biotechnology (BSB), ICHE approval 6/04, launched 8/04 Service: School and University: IUPUI Faculty Council, 1976-1978; 1981-1983, 1991-93 Nominating Committee, IUPUI Faculty Council, 1982-1983 Purdue System Curriculum Committee, 1978-1980 IUPUI Academic Procedures Committee, 1984-1987 Faculty Council Subcommittee on Facilities Utilization, 1985 Steering Committee for Campus Development, 1976-1977 Long Range Planning Committee, 1976-1979 Campus Development Committee, 1976-1979 2

Chairperson, Faculty Council Committee on Resources and Planning, l977-1978 Resources and Planning Committee, 1976-1977, 1978-1979 IUPUI Faculty Board of Review II, 1984 Faculty Council Athletic Affairs Committee, 1983-1985 IUPUI Honors Council, 1988-present Chair, 1993-2007 IUPUI Graduate Council Curriculum Subcommittee 1988-1989 Chairperson IUPUI Graduate Council Curriculum Subcommittee 19891990 Scholastic Delinquencies and Readmission Committee, School of Engineering and Technology, 1973-1976 Scholastic Delinquencies and Readmission Committee, School of Science, 1974-1976, 1989-1995 Search and Screen, Assistant Dean, 1982 School of Science Bulletin Committee, 1982-1997 School of Science Service Committee, 1986-1987 School of Science Academic Appeals Committee (Chair) 1987 School of Science Committee on Academic Counseling 1987 Fall Convocation Committee, 1986 School of Science Brochure Development Committee, 1991 School of Science Chairmans Council, 1992 - present Search and Screen Committee, Geology Chair, 1992-93 New Faculty Orientation Committee, 1993-2000 Executive Committee, Biomechanics and Biomaterials Research Center (BBRC) PhD Program 1994-95 Science and Education Committee, 1993 IUPUI Grade Inflation Committee, 1994-96 Science and Medicine Collaborative Programs Committee, 1994-1998 Campus Consolidation Planning Committee, 1995-96 Strategic Directions Discussion Group on Assessment, 1995-96 Science and Liberal Arts ad hoc Chair Group, 1995 Tissue Regeneration and Artificial Tissues Planning Committee, 1995-98 Campus Task Force on Research and Graduate Education, 1996-97 Committee on the Individually Designed Major, 1996-98 Chancellor's Fall Convocation Committee, 1996-1999 Campus Group of Chairs, 1994-present Advisory Board, Center for Earth and Environmental Sciences (CEES), ex officio, 1996-1998 Steering Committee, Program in Cellular and Integrative Biology (CIB), ex officio, 1996-98 Kellogg Project, Peer Review of Professional Service, Review Committee, 1997 Pew Charitable Trusts Grant, IUPUI Advisory Committee, 1997 Search & Screen, School of Liberal Arts Dean, 1997-98 Biomedical Engineering (BME) Advisory Committee, 1997-2003 BME Undergraduate Curriculum Committee 2001-present Search & Screen, University College Dean, Associate Dean, and Assistant Dean, (Chair), 1997-98 3

Search & Screen, Director of Honors, 1998 Faculty and Librarian Review & Enhancement Planning Team (Convener), (Post-Tenure Review) 1998-2002 School of Science Unit (Promotion & Tenure) Committee, 1998-99, 2003-04 Freshman Scholarship Review and Interview Committee, 1999-present School of Science Post-Tenure Review Implementation Committee (Chair), 1999 University College Collaborative Governance Group, 1999-2000 Dean's Advisory (Leadership) Council, 2000-2006 Gateway Group, promoting best practices in student retention, 2000-2006 Primary Committee (Chair), Department of Computer Science, 2000 Primary Committee, Department of Computer Science, 2004-2007 University College Curriculum Committee 2000-01 School of Science Animal Facility Committee, 2000-10 Review Committee, Dean of University College, 2001-02 Purdue University Strategic Planning Task Force, 2001 Connect Tech Day Planning Committee, 2001 School of Liberal Arts Individualized Major Program Committee, 2001-present University College Academic Policies & Procedures Committee (Chair) 2001-2006 Office of Professional Development Learning Community on Faculty Work 2002-03 University College (Campus) Undergraduate Admissions Committee (Chair) 2002-present Search & Screen, Associate Director of Honors, 2002 Bepko Scholars and Fellows Program Committee, 2003 Bepko Scholars and Fellows Selection Committee, 2004, 2006-present Administrative Review for the Director of the Honors Program, 2003-04 (Chair) University College Executive Committee, 2003-2005 Enrollment Management Council, 2004-present Enrollment Management Steering Committee, 2004-present Search & Screen, Associate Dean, School of Science, 2004 Search & Screen, Forensic Biologist, 2004-05 Campus Action Team Committees, 2006-07 Guaranteed Professional Program Admissions (GPPA) Student Employment (Chair) Masters Degree Programs Space Shortage Department of Earth Sciences PhD Program Committee, 2007-8 School of Science Strategic Planning: Administrative Team (Chair), 2007 Search & Screen, IUPUI Director of Admissions, 2007 Search & Screen (Chair), Chairperson of Chemistry & Chemical Biology, 2007 Search & Screen, Dean of Science, 2007-08 Search & Screen, Department of Earth Sciences, 2008 Program Review Committee, IUPUI Honors Program, 2008 Search & Screen, Dean of the IUPUI Honors College, 2008-09 University College 10th Anniversary Task Force, 2008-09 University College Task Force: Connections with Degree Programs, 2008 Committee for the Revision of the Program Review Guidelines, 2008-10 4

Crispus Attucks Medical Magnet High School (CAMMHS) Advisory Board, 2009-. Council on Retention and Graduation, 2009Search & Screen (Chair), Chairperson of Physics, 2009 Primary Committee, Department of Earth Sciences, 2009 Task Force for the Review of Science Signature Centers, 2009-10 Search & Screen, Forensic Biologist, 2009-10 Task Force on Student Employment, 2010-11 Primary Committee, Department of Chemistry & Chemical Biology, 2010-11 Search & Screen (Chair), Chairperson of Chemistry & Chemical Biology, 2010-11 Third-Year Committee (Chair), IU School of Dentistry faculty member, 2011 Departmental Service: Department Primary Committee, chair, 1992-2010, member, 2010Bulletin Committee, 1981-98 Curriculum Committee, 1979-1996 Department Class Scheduling, 1979-present Brochure Committee Chairperson, 1981 Liaison-Purdue System Biology Departments, 1982-present Reappointment, Tenure & Promotion Committee, 1975-77, 1979-1981, 1982-present Chair, 1992 2010, 2010Director of Department Undergraduate Admissions, Audits, and Degree Certifications, 1983-present Director of Graduate Programs, 1984-2010 Search and Screen-Biology Chair, 1976, 1985 Department Review Committee, 1985 Graduate Committee Chairperson, 1984-2010, member 2010Search and Screen- several including most recently - Molecular Biologist, 1989-1991, Cell/Molec. Biology, 1993-94, Anatomy and Physiology Lecturers, 1996, IUPUC Lecturer, 1997, Forensic Biologist, 2009-10, Multiple Faculty, 2009-10 (Chair) Committee for Graduate and Undergraduate Recruitment, 1991 Advising: Undergraduate: Biology Program Pre-pharmacy, Pre-optometry 198097, Graduate Program, 1984-2010. Professional Service: Indiana Academy of Science section vice chair (Microbiology and Molecular Biology), 1990-1991 Indiana Academy of Science section chair (Microbiology and Molecular Biology), 1991-1992 Local Arrangements Committee Co-chair, 1995 annual meeting of the Indiana Academy of Science, 1993-95 Section Co-Chair (Microbiology), Intel International Science and Engineering Fair, Indianapolis, IN, May, 2006 Grants (funded): IUPUI Commitment to Excellence Fund, Life-Health Sciences Research Internships at IUPUI: Extending the Engagement with Simon Rhodes, 2006-present, $250,000 per year. IUPUI Commitment to Excellence Fund, "The Freshman Work Program: A Tool for 5

Retention and Recruitment", 2003-present, $50,000 year 1, $100,000 year 2 and beyond. IUPUI Office of Student Scholarships, "Linking Student Work and Student Learning: A Mechanism for Recruitment and Retention", 2002-03, $17,000. IUPUI Office of Professional Development, Travel Award, 2001, $750 IUPUI Office of Student Scholarships, "Linking Student Work and Student Learning: A Mechanism for Recruitment and Retention", 2001-02, $16,000. IUPUI Office of Student Scholarships, "Linking Student Work and Student Learning: A Mechanism for Increased Retention", 2000-01, $10,000. IUPUI Faculty Development Travel Award, 1998, $400. U. S. Army, Defense Women's Health Research Program, "Determining Antifungal Target Sites in the Sterol Pathway of the Yeasts Candida and Saccharomyces", 1995-99, $218,846, Co-PI with M. Bard IUPUI Faculty Development Travel Award, 1995, $500. Johnson & Johnson Focused Giving Program, "Cloning of Yeast Sterol Genes for the Purpose of Identifying New Targets for Antifungal Drugs" Co-PI with M. Bard. 1992-95. $150,000. School of Science Interdisciplinary Grant, 1987 PDP Grant, 1982 Purdue XL Summer Faculty Fellowship, 1981 Participant, Department Undergraduate Research Participation (URP) Grant, NSF, 1980 IUPUI Grant-in-Aid, 1980 Biomedical Sciences Research Grant, 1977 Biomedical Sciences Research Grant, 1975 IU Summer Faculty Fellowship, 1975 IU Foundation Supply Grant, 1974 Books: Lees, N. D., Chairing Academic Departments Traditional and Emerging Expectations, Bolton, MA, Anker Publishing (now held by Jossey-Bass), 2006, 352 pgs. ISBN 1933371-03-x. Research Publications: Lees, N. D. and Welker, N. E. Restriction and Modification in Bacillus stearothermophilus. J. Virol. 11: 606-609, 1973. Catterall, J. F., Lees, N. D., and Welker, N. E. Restriction and Modification in Thermophilic Bacilli. Microbiology 1976: 358-366, 1976. Bard, M., Lees, N. D., Burrows, L. S., and Kleinhans, F. W. Differences in Crystal Violet uptake and Cation-induced Death among Yeast Sterol Mutants. J. Bact. 135: 1146-1148, 1978. Kleinhans, F. W., Lees, N. D., Bard, M., Haak, R. A., and Woods, R. A. ESR

Determinations of Membrane Permeability in a Yeast Sterol Mutant. Chem. Phys. Lipids 23: 143-154, 1979. Lees, N. D., Bard, M., Kemple, M. D., Haak R. A., and Kleinhans, F. W. ESR Determinations of Membrane Order Parameter in Yeast Sterol Mutants. Biochim. Biophys. Acta 553: 469-475, 1979. Bard, M., Neuhauser, J. L., and Lees, N. D. Caffeine Resistance of Saccharomyces cerevisiae. J. Bact. 141: 999-1002, 1980. Lees, N. D., Lofton, S. L., Woods, R. A. and Bard, M. The Effects of Varied Energy Source and Detergent on the Growth of Sterol Mutants of Saccharomyces cerevisiae. J. Gen. Microbiol. 118: 209-214, 1980. Lees, N. D., Kemple, M. D., Barbuch, R. J., Smith, M. A. and Bard, M. Differences in Membrane Order Parameter and Antibiotic Sensitivity in Ergosterol-producing Strains of Saccharomyces cerevisiae. Biochim. Biophys. Acta 776: 105-112, 1984. Bard, M., Lees, N. D., Barbuch, R. J., and Sanglard, D. Characterization of a Cytochrome P-450 Deficient Mutant of Candida albicans. Biochem Biophys. Res. Commun. 147: 794-800, 1987. Bard, M., Lees, N. D., Burnett, A. S., and Parker, R. A. Isolation and Characterization of Mevinolin Resistant Mutants of Saccharomyces cerevisiae. J. Gen. Microbiol. 134: 1071-1078, 1988. Lees, N. D., Kleinhans, F. W., Broughton, M. C., Pennington, D. A., Ricker, V. A. and Bard, Martin. Membrane Fluidity Alterations in a Cytochrome P450deficient Mutant of Candida albicans. Steroids 53: 567-578, 1989. Lees, N. D., Broughton, M. C., Sanglard, D., and Bard, M. Azole Susceptibility and Hyphae Formation in a Cytochrome P450-deficient Mutant of Candida albicans. Antimicrob. Agents and Chemother. 34: 831-836, 1990. Broughton, M. C., Bard, M., and Lees, N. D. Polyene Resistance in Ergosterol Producing strains of Candida albicans. Mycoses 34: 75-83, 1991. Lees, N. D., Arthington, Beth A., and Bard, M.. The Genetics and Molecular Biology of the Genes Functioning Late in the Sterol Biosynthetic Pathway of Saccharomyces. Regulation of Isopentenoid Metabolism. Eds. W. D. Nes, E. J. Parish, and J. M. Trzaskos. ACS Symposium Series 497: 246-259, 1992. Bard, M, Lees, N. D., Turi, T., Craft, D., Cofrin, L., Barbuch, R., Koegle, C., and Loper, J. C. Sterol Synthesis and Viability of erg11 (Cytochrome P450 lanosterol demethylase) Mutations in Saccharomyces cerevisiae and Candida albicans. Lipids 28: 963-967, 1993. Lees, N. D., Skaggs, B., Kirsch, D. R., and Bard, M. Cloning of the Late 7

Genes in the Ergosterol Biosynthetic Pathway of Saccharomyces cerevisiae - A Review. Lipids, 30: 221-226, 1995. Bard, M., Bruner, D. A., Pierson, C. A., Lees, N. D., Biermann, B., Frye, L., Koegel, C., and Barbuch, R. Cloning and Characterization of ERG25, the Saccharomyces Gene Encoding the C-4 Sterol Methyl Oxidase. Proc. Nat. Acad. Sci. USA 93: 186-190, 1996. Lees, N. D., Bard, M., and Kirsch, D. Biochemistry and Molecular Biology of Sterol Synthesis in Saccharomyces cerevisiae. In: Biochemistry and Function of Sterols. Eds. E. J. Parish and W. D. Nes. CRC Press, pp. 85-99, 1997. Pierson, C. A., Gachotte, D., Lees, N. D., Barbuch, R., Koegel, C., and Bard, M. A Yeast Sterol Auxotroph (ERG25) is Rescued by Addition of Azole Antifungals and Reduced Levels of Heme. Proc. Nat. Acad. Sci. USA 94: 1117311179, 1997. Jensen-Pergakes, K. L., Kennedy, M. A., Lees, N. D., Barbuch, R., Koegle, C., and Bard, M. Sequencing, Disruption, and Characterization of the Candida albicans Sterol Methyltransferase (ERG6) Gene: Susceptibility Studies in erg6 Mutants. Antimicrob. Agents Chemother. 42: 1160-1167, 1998. Daum, G., Lees, N. D., Bard, M., and Dickson, R. Biochemistry, Cell Biology and Molecular Biology of Lipids of Saccharomyces cerevisiae. Yeast 14: 1471-1510, 1998. Kennedy, M. A., Johnson, T. A., Lees, N. D., Barbuch, R., Eckstein, J. A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 Sterol Methyl Oxidase Gene (ERG25) and Expression of ERG25 Conditional Lethal Mutants in Saccharomyces cerevisiae. Lipids, 35: 257-262, 2000. Aaron, K. E., Pierson, C. A., Lees, N. D. and Bard, M. The Candida albicans ERG26 Gene Encoding the C-3 Sterol Dehydrogenase (C-4 decarboxylase) is Essential for Growth. FEMS Yeast Res., 1(2): 93-101, 2001. Jia, N., Arthington-Skaggs, B., Lee, W., Pierson, C.A., Lees, N.D., Eckstein, J., Barbuch, R., and Bard, M. The Characterization of the Candida albicans Sterol C14 Reductase, Encoded by the ERG24 Gene, as a Potential Antifungal Target Site. Antimicrob. Agents Chemother., 46: 947-957, 2002. Lees, N. D. and Bard, M. Sterol Biochemistry and Regulation in the Yeast Saccharomyces cerevisiae. Top. Cur. Genet. 6: 213-240, 2003. Rogers, K. M., Pierson, C. A., Culbertson, N. T., C. Mo, A. M. Sturm, Eckstein, J., Barbuch, R., Lees, N. D., and Bard, M. Disruption of the Candida albicans CYB5 Gene Results in Increased Azole Sensitivity. Antimicrob. Agents Chemother., 48: 3425-3435, 2004. Pierson, C. A., Jia, N., Mo, C., Lees, N. D., Sturm, A. S., Barbuch, R., Eckstein, J., 8

and Bard, M. Isolation, Characterization and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-keto Reductase. Med. Mycol., 42: 461-473, 2004. Bard, M., Sturm, A. M., Pierson, C. A., Brown, S., Rogers K. M., Nablinger, S., Eckstein , R. Barbuch R., Lees, N. D., Howell S. A., and Hazen, K. C. Sterol Uptake in Candida glabrata: Rescue of Sterol Auxotrophic Strains. Diagnos. Microbiol. Infect. Dis., 52: 285-293, 2005. Special Citations of Administrative Contributions: The following published works were disseminated through the Tomorrows Professor Email Newsletter from the Center for Teaching and Learning at Stanford University. Each citation was comprised of excerpted quotes, author biographies and a full text of the contribution. The Newsletter is distributed to over 25,000 subscribers at 650 institutions in 100 countries. TP Message #802, 6/1/07, N. D. Lees, Guiding Faculty Careers in: Chairing Academic Departments - Traditional and Emerging Expectations, Chapter 15, pages 197222, 2006. TP Message #846, 2/13/08, D. J. Malik and N. D. Lees, Working Effectively with the Dean, The Department Chair, 18(3): 1-3, 2008. TP Message #924, 2/24/09, D J. Malik and N. D. Lees, The Accountability Movement: Its Role, Opportunities and Meaning for Chairs, The Department Chair, 19(3): 3-5, 2009. TP Message #1038, 9/4/10, N. D. Lees, Suggestions for Making Chair Work More Satisfying and Attractive, The Department Chair, 21(1): 18-20, 2010. The following published works are available at the Department Chair Online Resources Center (http://www.acenet.edu/resources/chairs/) sponsored by the American Council on Education (ACE). Malik, David J., and N. Douglas Lees. Making Faculty Evaluation Count, The Department Chair 11(2): 6-8, 2000, posted 6/26/03. Barr, Susan H., N. Douglas Lees, and Betsy E. Brown. Preparing Chairs for Expanded Roles in Post-Tenure Review: New Perspectives for Chairs, The Department Chair 11(2): 9-10, 2000, posted 6/26/03. Jenski, Laura J. and N. Douglas Lees. Developmental Models for Chair Workload Delegation, The Department Chair 12(1): 17-18, 2001, posted 6/26/03. Jenski, Laura J. and N. Douglas Lees. Department Planning Within the Context of Institutional Expectations, The Department Chair 13(4): 14-17, 2003, posted 11/4/03.

Lees, N. Douglas. Retaining and Graduating Undergraduates, The Department Chair 16(4): 5-7, 2006, posted 12/15/06. Lees, N. Douglas. Chairing Academic Departments: Traditional and Emerging Expectations, ISBN 1-933371-03-x, table of contents and brief description only, posted 12/15/06. Malik, David J. and Lees, N. Douglas. The Accountability Movement: Its Role, Opportunities and Meaning for Chairs, The Department Chair, 19(3): 3-5, 2009, posted 7/21/09. Lees, N. D., Malik, D. J. and Vemuri, G. Essentials of Chairing Academic Departments, The Department Chair, 20(2): 1-3, 2009, posted 11/30/09. ScienceCareers (a publication of AAAS) citations: Based on: N. D. Lees, Guiding Faculty Careers in: Chairing Academic Departments Traditional and Emerging Expectations, Chapter 15, pages 197-222, 2006, an interview resulted in the publication at: http://sciencecareers.sciencemag.org/career_magazine/previous_issues/articles/2 009_06_26/caredit.a0900081 A major reference: Lees, N. Douglas. Chairing Academic Departments: Traditional and Emerging Expectations, ISBN 1-933371-03-x, was listed as a major resource in: http://sciencecareers.sciencemag.org/career_magazine/previous_issues/articles/2 009_06_26/caredit.a0900080 Postings on the The Jossey-Bass Department Chair Insider, an email resource for department chairs, under Ask the Department Chair Insider: September 2010, Vol. 1 How can chairs meet the increased expectations of their position? January 2011, Vol. 1 What are some options for chairs to continue their teaching and research activities? Administrative and Other Publications: Lees, N. D., and Malik, D. J. Fostering Elements of Change in a Department. The Department Chair 6(1): 1, 19-20, 1995. Malik, D. J., and Lees, N. D. Departments in Transition: The Role of Faculty and Program Evaluation, Proceedings of Twelfth Annual Conference Academic Chairpersons: The Many Faces of Evaluation, 45: 108-118, 1995. Lees, N. D., and Malik, D. J. Faculty Workload and Accountability:

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Considerations in the Development of Practical Models. Proceedings of the Thirteenth Annual Conference Academic Chairpersons: Approaches to Accountability, 46: 151-162, 1996. Lees, N. D., and Malik, D. J. Maintaining Teaching Balance in the Department. The Department Chair, 7(3): 11-13, 1997. Malik, D. J., and Lees, N. D. Program Review: A Mechanism for Enhancing Academic Opportunity in an Era of Change. Proceedings of the Fourteenth Annual Conference Academic Chairpersons: Changing Answers to Recurring Questions, 47: 163-172, 1997. Lees, N. D., and Malik, D. J. Can External Review Catalyze Department Change? The Department Chair, 8(1): 13-14, 1997. Lees, N. D., and Malik, D. J. Establishing a Campus Chair Community: Collaboration for Change. Proceedings of the Fifteenth Annual Conference The Academic Department: The Cornerstone of Higher Education, 48: 122-128, 1998. Turner, R. C., Lees, N. D., Mullen, E. T., Malik. D. A., and Ford, D., Creating a Community of Chairs. The Department Chair, 9(2): 9-10. 1998. Lees, N. D. and Malik, D. J. Representing the Department Effectively to its Constituencies: Precursors to Change. Proceedings of the Sixteenth Annual Conference Transforming the Academic Department. 49: 165-174, 1999. Lees, N. D. and Malik, D. J. Creating a Link between Accountability and Department Improvement. The Department Chair, 10(1): 1, 18-19, 1999. Lees, N. D., Hook, S., and Powers, G. Post-Tenure Review: Changes for Faculty and Challenges for Department Chairs, The Department Chair, 10(2): 7-8, 1999. Lees, N. D. and Malik, D. J. Faculty Evaluation and the Chair Role: Applications of Workload-Based Measures for Merit Pay and Post-Tenure Review, Proceedings of the Seventeenth Annual Conference Celebrating Success: Sharing Best Practices, 50: 143-152, 2000. Barr, S. H., Lees, N. D., and Brown, B. Preparing Chairs for Expanded Roles in Post-Tenure Review: New Perspectives for Chairs, The Department Chair, 11(2): 9-10, 2000. Malik, D. J. and Lees, N. D. Making Faculty Evaluation Count, The Department Chair, 11(2): 6-8, 2000. Hook, S. A., Lees, N. D., and Powers, G. Post-Tenure Review (SPEC Kit 261). Association of Research Libraries, Washington, DC, 121 pages, October 2000. Lees, N. D., Brown, B., and Barr, S. H. Preparing Chairs for Expanded 11

Roles in Post-Tenure Review: Faculty Evaluation and the Annual Review, The Department Chair, 11(3): 7-9, 2001. Brown B., Barr, S. K., and Lees, N. D., Preparing Chairs for Expanded Roles in Post-Tenure Review: Providing Feedback to Faculty, The Department Chair, 11(4): 7-9, 2001. Lees, N. D. and Jenski, L. J. Reducing Chair Workload and Enhancing Department Services, Proceedings of the Eighteenth Annual Academic Chairpersons Conference The 21st Century Department: New Challenges, New Solutions, 51: 101-109, 2001. Lees, N. D. and Malik, D. J. Preparing New Chairs, Proceedings of the Eighteenth Annual Academic Chairpersons Conference The 21st Century Department: New Challenges, New Solutions, 51: 93-100, 2001. Jenski, L. J. and Lees, N. D. Developmental Models for Chair Workload Delegation, The Department Chair, 12(1): 17-18, 2001. Lees, N. D. and Malik, D. J. Move over Faculty Development: Let Chairs Train Chairs, The Department Chair, 12(2): 10-12, 2001. Lees, N. D. The Evolution of Post-Tenure Review at Indiana University Purdue University Indianapolis, In: Post-Tenure Faculty Review and Renewal: Experienced Voices, (Licata, C. M. and Morreale, J. C., eds.), American Association for Higher Education, Washington, DC, pages 97-110, 2002. Evenbeck, S., Lees, N. D., and Chism, N. Campus to Department Collaboration in Promoting Student Success: The Critical Role of Department Chairs in Retention Initiatives, The Department Chair, 12(4): 24-26, 2002. Lees, N. D. and Jenski, L. J. Balancing Chair and Faculty Workloads in Academic Departments, Academic Leader, 18(4): 1,7, 2002. Lees, N. D. and Jenski, L. J. A Nested Mission & Goals Approach to Department Strategic Planning, Proceedings of the Nineteenth Annual Academic Chairpersons Conference The Changing Role of Department Chairs, 52: 113-121, 2002. Malik, D. J. and Lees, N. D. Tips to the Department Chair, Proceedings of the Nineteenth Annual Academic Chairpersons Conference The Changing Role of Department Chairs, 52: 123-131, 2002. Chism, N. V., Lees, N. D., and Evenbeck, S. Faculty Development for Teaching Innovation through Communities of Practice, Liberal Education, 88(3): 34-41, 2002. Lees, N. D. and Malik, D. J. Strategies for Successful Chairing. Public Work, The Department Chair, 13(3): 13-15. 2003.

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Jenski, L. J. and Lees, N. D. Department Planning within the Context of Institutional Expectations, The Department Chair, 13(4): 14-17, 2003. Malik, D. J. and Lees, N. D. Strategies for Successful Chairing. Private Work and Scholarship, The Department Chair, 13(4): 1-3, 2003. Jenski, L. J., Walczak, M., and Lees, N. D. Selecting New Chairs: Matching Institutional and Applicant Expectations, Proceedings of the Twentieth Annual Academic Chairpersons Conference Visions of Departmental Leadership, 53: 145153, 2003. Lees, N. D. and Malik, D. J. Chair Leadership Beyond the Department, Proceedings of the Twentieth Annual Academic Chairpersons Conference Visions of Departmental Leadership, 53: 163-172, 2003. Jenski, L. J., Walczak, M. M., and Lees, N. D. Defining Institutional and Applicant Expectations During Chair Selection, The Department Chair, 14(1): 3,6-7, 2003. Lees, N. D. and Hook, S. A. Post-Tenure Review at Indiana University Purdue University Indianapolis: A Three Year Status Report, In: Post-Tenure Faculty Review and Renewal II: Reporting Results & Shaping Policy, (Licata, C. M. and Brown, B. E. eds.), Anker Publishing, Bolton, MA, pages 163-170, 2004. Malik, D. J. and Lees, N. D. Global Leadership and the Department Chair, The Department Chair, 14(3): 1-3, 2004. Lees, N. D. Raising the Bar for Department Chairs, The Department Chair, 14(4): 9-10, 2004. Jenski, L. J. and Lees, N. D. Adding Ideas, Subtracting Criticism, Dividing Work, and Other Ways to Count on Advisory Groups, The Department Chair, 15(1): 13-15, 2004. Jenski, L. J. and Lees, N. D. Using Advisory Committees to Promote Department Needs and Objectives, Proceedings of the Twenty First Annual Academic Chairpersons Conference Choosing the Right Path among Many Options: Creating a Meaningful Niche, 54: CD, 6 pages, 2004. Lees, N. D. and Malik, D. J. Creating External Partnerships to Enhance Department Relevance, Image and Fiscal Stability, Proceedings of the Twenty First Annual Academic Chairpersons Conference Choosing the Right Path among Many Options: Creating a Meaningful Niche, 54: CD, 8 pages, 2004. Lees, N. D. Taking the Department Chair Position Seriously, The Department Chair, 15(2): 1-3, 2004. Lees, N. D. The Department Chair Position as a Step in a Career Path, The Depart-

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ment Chair, 15(3): 3-5, 2005. Lees, N. D. and Brown, B. E. Staffing Academic Departments, Proceedings of the Twenty Second Annual Academic Chairpersons Conference, The Chairs Role in Empowering Change, 55: CD, 16 pages, 2005. Jenski, L. J. and Lees, N. D. Using Public Information Forums to Generate Support for New Initiatives, Proceedings of the Twenty Second Annual Academic Chairpersons Conference, The Chairs Role in Empowering Change, 55: CD, 5 pages, 2005. Jenski, L. J., Lees, N. D. and Harrison, M. A. Getting Attention For New Initiatives Without Making It Just More Work!, The Department Chair, 16(1): 15-17, 2005. Brown, B. E. and Lees, N. D. Planning Departments of the Future, The Department Chair, 16(1): 3-6, 2005. Lees, N. D. and Malik, D. J. Collaborative External Partnerships: Improving Department Finances and Image, The Department Chair, 16(2): 18-20, 2005. Lees, N. D. Chair Leadership in Campus and Department Initiatives in Student Retention, Proceedings of the Twenty Third Annual Academic Chairpersons Conference, Changing Leadership: Expectations for Chairs 56: CD, 8 pages, 2006. Lees, N. D. Retaining and Graduating Undergraduates: New Leadership Roles for Department Chairs, The Department Chair, 16(4): 5-7, 2006. Lees, N. D. and Licata C. M. Faculty Evaluation: Continuing Challenges for Chairs, Proceedings of the Twenty-Third Annual Academic Chairpersons Conference, Changing Leadership: Expectations for Chairs 56: CD, 10 pages, 2006. Licata, C. M. and Lees, N. D. The Chair as Evaluation Charlatan, The Department Chair, 17(2): 16-18, 2006. Lees, N. D. Academic Advising: Choosing a Model That Works for Your Department, Proceedings of the Twenty-Fourth Academic Chairpersons Conference, Academic Leadership: Building Responsive and Responsible Communities, 57: CD, 11 pages, 2007. Lees, N. D. Rethinking Academic Advising, The Department Chair, 17(4): 17-20, 2007. Lees, N. D. and Malik, D. J. Interacting with the Dean, Proceedings of the TwentyFourth Academic Chairpersons Conference, Academic Leadership: Building Responsive and Responsible Communities, 57: CD, 8 pages, 2007. Lees, N. D. Maintaining Disciplinary Competitiveness While Chair, The Department Chair, 18(1): 9-10, 2007.

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Malik, D. J. and Lees, N. D. Working Effectively with the Dean, The Department Chair, 18(3): 1-3, 2008. Lees, N. D. A review of: Campus Crisis Management: A Comprehensive Guide to Planning, Prevention, and Recovery, Eugene L. Zdziarski II, Norbert W. Dunkel, J. Michael Rollo, and Assoc., The Department Chair, 18(4): 29-31, 2008. Lees, N. D. and Vemuri, G. Interdisciplinarity: Moving Beyond Traditional Boundaries, Proceedings of the Twenty-Fifth Academic Chairpersons Conference, Defining Department Leadership: Engaging Academic Communities for Success, 58: CD, 10 pages, 2008. Lees, N. D. and Malik, D. J. Accountability and the Role of the Department Chair, Proceedings of the Twenty-Fifth Academic Chairpersons Conference, Defining Department Leadership: Engaging Academic Communities for Success, 58: CD, 9 pages, 2008. Lees, N. D. and Vemuri, G. Creating Department Advantage through Interdisciplinary Ventures, The Department Chair, 19(2): 16-18, 2008. Malik, D. J. and Lees, N. D. The Accountability Movement: Its Role, Opportunities and Meaning for Chairs, The Department Chair, 19(3): 3-5, 2009. Lees, N. D. and Vemuri, G. Faculty Recruitment: Trends, Challenges and Opportunities, Proceedings of the Twenty-Sixth Academic Chairpersons Conference, What is on the Horizon, 59: CD, 9 pages, 2009. Lees, N. D., Vemuri, G and Malik, D. J. Advice for Department Chairs, Proceedings of the Twenty-Sixth Academic Chairpersons Conference, What is on the Horizon, 59: CD, 10 pages, 2009. Lees, N. D. and Vemuri, G. New Challenges in Faculty Recruiting, The Department Chair, 20(1): 3-4, 2009. Lees, N. D. and Malik, D. J. Facilitating and Enhancing Research and Scholarship, Proceedings of the Twenty-Sixth Academic Chairpersons Conference, What is on the Horizon, 59: CD, 9 pages, 2009. Lees, N. D., Malik, D. J. and Vemuri, G. Essentials of Chairing Academic Departments, The Department Chair, 20(2): 1-3, 2009. Gilbert, B. L., Lees, N. D. and Rhodes, S. J. The Life-Health Sciences Internships Program: Research and Professional Experience Internships as an Undergraduate Retention Tool. In: R. Hayes (Ed.), Proceedings of the 5th National Symposium on Student Retention, 5: 404-410, 2009. Lees, N. D. and Malik, D. J. Are Research & Scholarship and Higher Education Reform Compatible Concepts?, The Department Chair, 20(3): 4-7, 2010.

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Lees, N. D. Structuring Chair Work for Long-term Productivity and Personal Satisfaction, Proceedings of the Twenty-Seventh Academic Chairpersons Conference, A New Generation of Accountability, 60: CD, 9 pages, 2010. Lees, N. D. Suggestions for Making Chair Work More Satisfying and Attractive, The Department Chair, 21(1): 18-20, 2010.

Lees, N. D. Review of Good Mentoring: Fostering Excellent Practice in Higher

Education, Mentoring & Tutoring: Partnership in Learning 18(4): 439-443, 2010. Lees, N. D. Developing Persuasive Arguments for Resources, The Department Chair, 21(4): in press, 2011. Lees, N. D. Planning Your Escape: Exiting the Chair Position, Proceedings of the Twenty-Eighth Annual Academic Chairpersons Conference, Transforming Higher Education: The Department Chairs Role, 61: in press, 2011. Lees, N. D. Preparing for Your Departure as Chair, The Department Chair, 22(1): in press, 2011. Abstracts: Kleinhans, F. W., M. Bard, Lees, N. D., and R. A. Haak. Increased Permeability of Yeast Membrane Mutants to Ni++ as Detected by ESR. Bull. Amer. Phys. Soc. 22: 77, 1977. Kemple, M. D., F. W. Kleinhans, Lees, N. D., and M. Bard. EPR Study of Yeast Sterol Mutants. Bull. Amer. Phys. Soc. 24: 346, 1979. Bard, M., Lees, N. D., Kleinhans, F.W., Barbuch, R., and Sanglard, D. Characterization of a Cytochrome P450 Deficient Mutant of Candida albicans. Proc. Ind. Acad. Sci. 103: 64, 1987. Broughton, M. C., Lees, N. D., and Bard, M. Azole Sensitivity and Hyphal Formation of a Cytochrome P450-deficient Mutant of Candida albicans. Proc. Ind. Acad. Sci., 104: 53, 1988. Broughton, M. C., Bard, M., and Lees, N. D. Polyene Resistance in Ergosterol-producing Strains of Candida albicans. Proc. Ind. Acad. Sci., 106: 61, 1990. Craft, D., Turi, T., Loper, J. C., Barbuch, R., Koegle, C., Cofrin, L., Lees, N. D., and Bard, M. Sterol Synthesis and Viability of erg11 (Cytochrome P450 lanosterol demethylase) Mutations in Saccharomyces cerevisiae and Candida albicans. Yeast Genetics and Molecular Biology meeting, Univ. Wisconsin, 1993. Malik, D. J. and Lees, N. D. Departments in Transition: The Role of Faculty

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and Program Evaluation, 12th Annual Academic Chairpersons Conference, Academic Chairpersons: The Many Faces of Evaluation, 12: 6. 1995. Johnson, B. G., Schoepp, D. D., and Lees, N. D. Measurement of receptormediated cytidine diphosphateidiacylglycerol formation in rat brain slices. Poster presentation, Society for Neuroscience Annual Meeting, Indianapolis, May 16, 1995. Bard, M., Bruner, D., Pierson, C.A., Lees, N. D., Biermann, B., Frye, L., Koegel, C., and Barbuch, R. The Cloning and Characterization of ERG25, the S. cerevisiae Gene Encoding C-4 Sterol Methyl Oxidase. Yeast Cell Biol. Mtg. Cold Spring Harbor, NY, p. 226, 1995. Lees, N. D. and Malik, D. J. Faculty Work: Approaches to Accountability, 13th Annual Academic chairpersons Conference, Academic Chairpersons: Approaches to Accountability, 13: 4. 1996. Malik, D. J. and Lees, N. D. Models for Faculty Workload Accountability. American Association for Higher Education, Forum on Faculty Roles & Rewards: Faculty Careers for a New Century. 4: 67, 1996. Kennedy, M. A., Pergakes, K. J., Lees, N. D., and Bard, M. Cloning of the Candida albicans ERG6 Gene. Proc. Ind. Acad. Sci. 112: 86-87, 1996. Johnson, T. A., Kennedy M. A., Lees, N. D., and Bard, M. Cloning of the Candida albicans ERG25 Gene. Proc. Ind. Acad. Sci. 112: 86, 1996. Malik, D. J and Lees, N. D. Program Review: A Mechanism for Enhancing Academic Opportunity in an Era of Change, 14th Annual Academic Chairpersons Conference, Academic Chairpersons: Changing Answers to Recurring Questions, 14: 4. 1997. Malik, D. J. and Lees, N. D. An Opportunity for Departmental Change through External Review. American Association for Higher Education, Forum on Faculty Roles & Rewards: Changing Realities and the Transformation of Faculty Work. 5: 37, 1997. Lees, N. D. and Malik, D. J. Establishing a Campus Chair Community, 15th Annual Academic Chairpersons Conference, Academic Departments: The Cornerstone of Higher Education, 15: 3. 1998. Lees, N. D., Mullen, E. T., Turner, R., Ford, D., and Malik, D. J. A Community of Chairs: Collaboration for Change. American Association for Higher Education, Forum on Faculty Roles & Rewards: Faculty Working in Learning Organizations. 6: 15, 1998. Lees, N. D., Malik, D. J., Ford, D., Turner, R., and Mullen, E. T. Creating a Community of Chairs. American Association for Higher Education, Forum on Faculty Roles & Rewards: Faculty Working in Learning Organizations. 6: 54, 1998. 17

Malik, D. J. and Lees, N. D. Representing the Department to its Constituencies: Precursors to Change, 16th Annual Academic Chairpersons Conference, Transforming the Academic Department, 16: 6. 1999. Lees, N. D., Hook, S., and Powers, G. Faculty and Librarian Review and Enhancement: A Developmental Plan for Post-Tenure Review, American Association for Higher Education, Forum on Faculty Roles & Rewards: The Academic Calling - Changing Commitments and Complexities. 7: 35. 1999. Lees, N. D. and Malik, D. J. The Chair and Faculty as Representatives to the Departmental Constituencies Can Lead to Change, American Association for Higher Education, Forum on Faculty Roles & Rewards: The Academic Calling Changing Commitments and Complexities. 7: 66. 1999. Kennedy, M. A., Johnson, T.A., Lees, N. D., Barbuch, R., Eckstein, J. A., and Bard, M. Cloning and Sequencing of the Candida albicans C-4 Sterol Methyl Oxidase Gene (ERG25) and Expression of an ERG25 Conditional Lethal Mutation in Saccharomyces cerevisiae. Inform 10: S70. 1999. Aaron, K., Lees, N., and Bard, M. Cloning and Disruption of the ERG26 Gene in the Pathogenic Yeast Candida albicans. Proc. Ind. Acad. Sci. 115: 70. 1999. Kennedy, M. A., Johnson, T. A., Lees, N. D., Barbuch, R., Eckstein, J. A., and Bard, M. Identification of the C-4 Sterol Methyl Oxidase Gene (ERG25) from Candida albicans and Expression of ERG25 Conditional Lethal Mutations in Saccharomyces cerevisiae. Proc. Ind. Acad. Sci. 115: 69. 1999. Brown, B., Barr S. H., and Lees N, D. Role of the Department Chair in PostTenure Review. Proc. Eighth Annual American Association for Higher Education Forum on Faculty Roles & Rewards: Scholarship Reconsidered Reconsidered. 8: 6. 2000. Lees, N. D. and Malik, D. J. Faculty Evaluation: Applications of WorkloadBased Measures for Merit Pay and Post-Tenure Review, 17th Annual Academic Chairpersons Conference, Celebrating Success: Sharing Best Practices, 17: 4. 2000. Jia, N., Randall, S., Lees, N., and Bard, M. Cloning and Disruption of the ERG27 and ERG24 Genes in the Yeast Candida albicans. Proc. Ind. Acad. Sci. 116: 80. 2000. Lees, N. D. and Jenski, L. J. Reducing Chair Workload and Enhancing Department Services, 18th Annual Academic Chairpersons Conference, The 21st Century Department: New Challenges, New Solutions, 18: 6. 2001. Lees, N. D. and Malik, D. J. Preparing New Chairs, 18th Annual Academic Chairpersons Conference, The 21st Century Department: New Challenges, New Solutions, 18: 7. 2001.

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Brown, B. E., Lees, N. D. and Barr, S. H. Post-tenure Review and the Department Chair: Providing Feedback and Promoting Faculty Development. Proc. Ninth Annual American Association for Higher Education, Forum on Faculty Roles & Rewards, The Changing Professoriate. 9: 80. 2001. Malik, D. J. and Lees, N. D. Tips to the Department Chair, 19th Annual Academic Chairpersons Conference, The Changing Role of Department Chairs, 19: 4. 2002. Lees, N. D. and Jenski, L. J. A "Nested Mission & Goals" Approach to Department Strategic Planning, 19th Annual Academic Chairpersons Conference, The Changing Role of Department Chairs, 19: 5. 2002. Barr, S. H., Brown B., and Lees N. D. Post-Tenure Review and the Department Chair: Engaging Faculty through Effective Written and Verbal Feedback, Proc. Tenth Annual American Association for Higher Education, Forum on Faculty Roles and Rewards: Knowledge for What? The Engaged Scholar. 10: 70. 2002. Lees, N. D., and Jenski, L. J. Strategies for Chairs in Opening Faculty Cultures to Change, Proc. Tenth Annual American Association for Higher Education, Forum on Faculty Roles and Rewards: Knowledge for What? The Engaged Scholar. 10: 50. 2002. Jenski, L. J., Walczak, M., and Lees, N. D. Selecting New Chairs: Matching Institutional and Applicant Expectations, 20th Annual Academic Chairpersons Conference, Visions of Departmental Leadership, 20: 5. 2003. Malik, D. J. and Lees, N. D. Chair Leadership Beyond the Department, 20th Annual Academic Chairpersons Conference, Visions of Departmental Leadership, 20: 4. 2003. Lees, N., and Chism, N. Playing Well with Others: A Community of Practice on Retention, AAHE Learning to Change Conference, Good work in Challenging Times, 1: 45. 2003. Lees, N. D., and Brown, B. Rethinking Faculty Appointments, AAHE Learning to Change Conference, Good work in Challenging Times, 1: 24. 2003. Pierson, C. A., Jia, N., Mo, C., Lees, N. D., Sturm, A. S., Eckstein, J., Barbuch, R., and Bard, M. Isolation, characterization and regulation of the Candida albicans ERG27 gene encoding the sterol 3-keto reductase. Proc. 15th Congress Soc. Human Animal Mycol. 15: 410. 2003. Jenski, L. J. and Lees, N. D. Using Advisory Groups to Promote Department Needs and Objectives, 21tst Annual Academic Chairpersons Conference, Choosing the Right Path among Many Options: Creating a Meaningful Niche, 21: 5. 2004. Lees, N. D. and Malik, D. J. Creating External Partnerships to Enhance

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Department Relevance, Image, and Fiscal Stability, 21tst Annual Academic Chairpersons Conference, Choosing the Right Path among Many Options: Creating a Meaningful Niche, 21: 6. 2004. Jenski, L. J. and Lees, N. D. Riding the Waves of Generational Change, AAHE Learning to Change Conference, Learning in 3D, 2: 36. 2004. Lees, N. D. Engagement through Collaboration: Transforming the Local Economy through Innovative Academic Programming, AAHE Learning to Change Conference, Learning in 3D, 2: 41. 2004. Lees, N. D. and Brown, B. E. Staffing Academic Departments, 22nd Annual Academic Chairpersons Conference, The Chairs Role in Empowering Change, 22: 3. 2005. Jenski, L. J. and Lees, N. D. Using Public Information Forums to Generate Support for New Initiatives, 22nd Annual Academic Chairpersons Conference, The Chairs Role in Empowering Change, 22: 6. 2005. Lees, N. D. The Freshman Work Program: A Tool for Student Success and Retention, AAHE National Conference on Higher Education, Rallying the Trendsetters in Higher Education, pp. 71-72, 2005. Lang, W., Joyce, J., Lees, N. D., Cothrel, A., and Lee, K. F. Creating a Community College System with the Help of Industry in Indiana: Preparing for a Scientific and Economic Revolution, BIO 2005, Community College Program, P. 2, 2005. Lees, N. D. Chair Leadership in Campus and Department Initiatives in Student Retention, 23rd Annual Academic Chairpersons Conference, Changing Leadership: Expectations for Chairs, 23: 6. 2006. Lees, N. D. and Licata, C. M. Faculty Evaluation: Continuing Challenges for Chairs, 23rd Annual Academic Chairpersons Conference, Changing Leadership: Expectations for Chairs, 23: 7. 2006. Malik, D. J and Lees, N. D. Interacting with the Dean, 24th Annual Academic Chairpersons Conference, Building Responsive and Responsible Communities, 24: 3. 2007. Lees, N. D. Academic Advising: Choosing a Model that Works for your Department, 24th Annual Academic Chairpersons Conference, Building Responsive and Responsible Communities, 24: 5. 2007. Lees, N. D. and Vemuri, G. Interdisciplinarity: Moving Beyond Traditional Boundaries, 25th Annual Academic Chairpersons Conference, Defining Departmental Leadership: Engaging Academic Communities for Success, 25: 5. 2008. Lees, N. D. and Malik, D. J. Accountability and the Role of the Department Chair,

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25th Annual Academic Chairpersons Conference, Defining Departmental Leadership: Engaging Academic Communities for Success, 25: 6. 2008. Gilbert, B.L., Lees, N.D., and Rhodes, S.J. Improving retention through the LifeHealth Sciences Internships program at Indiana University-Purdue University Indianapolis (IUPUI). In R. Hayes (Ed.), Proceedings of the 4th National Symposium on Student Retention, P. 366, 2008. Lees, N. D. and Vemuri, G. Faculty Recruitment: Trends, Challenges and Opportunities, 26th Annual Academic Chairpersons Conference, What is on the Horizon, 26: 3. 2009. Lees, N. D., Vemuri, G and Malik, D. J. Advice for Department Chairs, 26th Annual Academic Chairpersons Conference, What is on the Horizon, 26: 7. 2009. Lees, N. D. and Malik, D. J. Facilitating and Enhancing Research and Scholarship, 26th Annual Academic Chairpersons Conference, What is on the Horizon, 26: 8. 2009. Gilbert, B.L., Lees, N.D., and Rhodes, S.J. Improving Retention through the LifeHealth Sciences Internships Program at Indiana University-Purdue University Indianapolis (IUPUI). In: Proceedings of the 5th National Symposium on Student Retention, P. 404, 2009. Lees, N. D. Structuring Chair Work for Long-Term Productivity and Personal Satisfaction, 27th Annual Academic Chairpersons Conference, A New Generation of Accountability, 27: 4. 2010. Gilbert, B.L., Lees, N.D., and Rhodes, S.J. Bridging the Gap: The Life-Health Sciences Internship Program. Association of American Universities and Colleges (AAU&C). Creativity, Inquiry, and Discovery: Undergraduate Research In and Across the Disciplines. LEAP Featured Session, P. 10, 2010. Gilbert, B.L., Lees, N.D., and Rhodes, S.J. Educate, Engage, Enlighten: Learning Outcomes of Undergraduates Participating in the Life-Health Sciences Internship Program. Midwest Cooperative Education and Internship Association (MCEIA) Conference, 2010. Lees, N. D. Planning Your Escape: Exiting the Chair Position, 28th Annual Academic Chairpersons Conference, Transforming Higher Education: The Department Chairs Role, 28: 9. 2011. Recent Invited or Competitive Presentations: Departments in Transition: The Role of Faculty and Program Evaluation with D. Malik, "Academic Chairpersons: The Many Faces of Evaluation", 12th Annual Academic Chairpersons Conference, Orlando, FL, February, 1995.

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Cloning and Characterization of Genes in Sterol Biosynthesis and Metabolism for the Identification of New Antifungal Target Sites, with M. Bard, Johnson & Johnson Focused Giving Symposium, New Brunswick, NJ, November, 1995. Models for Faculty Workload Accountability with D. Malik, Fourth AAHE Conference, Forum on Faculty Roles & Rewards, Faculty Careers for a New Century, Atlanta, GA, January, 1996. Faculty Work: Approaches to Accountability co-presented workshop with D. Malik, "Academic Chairpersons: Approaches to Accountability", 13th Annual Academic Chairpersons Conference, Orlando, FL, February, 1996. The Biology One Year Pre-professional M.S. Degree at IUPUI, State-wide Conference for Pre-medical Advisors, Indiana University School of Medicine, Indianapolis, IN, September, 1996. Strategies and Surprises for New Administrators, Workshop, IUPUI Office of Faculty Development, with R. Turner and D. Malik, Indianapolis, IN, November 15, 1996. Program Review: A Mechanism for Enhancing Academic Opportunity in an Era of Change, with D. Malik, "Academic Chairpersons: Changing Answers to Recurring Questions", 14th Annual Academic Chairpersons Conference, Orlando, FL, February, 1997. An Opportunity for Departmental Change through External Review, with D. Malik, AAHE Conference, Forum on Faculty Roles & Rewards, Changing Realities and the Transformation of Faculty Work, San Diego, CA, January, 1997. A Plan to Enhance Student Success in Large Introductory Courses, co-presented with J. Kremer, R. Larter, and R. White, 1997 Indiana Institute, Indianapolis, IN, June, 1997. Professional Development of Chairs, round table with D. Malik and R. Turner, AAHE Summer Academy, Snowbird, UT, July, 1997. Establishing a Community of Chairs: A Prelude to Campus Change, Panel with R. Turner, D. Malik, and E. Mullen, "Conversation with Peg Miller, President of the AAHE", Indianapolis, IN, October, 1997. Establishing a Campus Chair Community, with D. Malik, "The Academic Department: The Cornerstone of Higher Education", 15th Annual Academic Chairpersons Conference, Orlando FL, February, 1998. A Community of Chairs: Collaboration for Change, with E. T. Mullen, R. Turner, D. Ford and D. Malik, American Association for Higher Education, Forum on Faculty Roles & Rewards, Faculty Working in Learning Organizations, Orlando FL, January, 1998. Creating a Community of Chairs, with D. Malik, D. Ford, R. Turner, and E. T. Mullen, American Association for Higher Education, Forum on Faculty Roles & Rewards, Faculty Working in Learning Organizations, Orlando FL, January, 1998.

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Biology at IUPUI and the Proposed Curriculum for a B. Sc. in Biological Science at the University of Qatar, University of Qatar, Doha, Qatar, April 1, 1998. Department Chairs as Agents of Change, with E. T. Mullen, American Association of Higher Education Summer Academy, Vail, CO, June, 1998. Faculty and Librarian Review and Enhancement: A Developmental Plan for Post-Tenure Review, with S. A. Hook, and G. Powers, American Association for Higher Education, Forum on Faculty Roles & Rewards, The Academic Calling: Changing Commitments and Complexities, San Diego, CA, January, 1999. The Chair and Faculty as Representatives to the Department Constituencies Can Lead to Change, with D. Malik, American Association for Higher Education, Forum on Faculty Roles & Rewards, The Academic Calling: Changing Commitments and Complexities, San Diego, CA, January, 1999. Representing the Department Effectively to its Constituencies: Precursors to Change, with D. Malik, "Academic Chairpersons: The Transformation of the Academic Department", 16th Annual Academic Chairpersons Conference, Orlando, FL, February, 1999. A Community of Chairs & Chair Preparation and Development, with D. Ford, University of Dayton, March, 1999. Academic Programs & Research in Biology at IUPUI, Dow AgroSciences, Indianapolis, IN, May 19, 1999. The IUPUI Biology One Year M.S.: Current Status and Five Year Report, State-wide Conference for Pre-medical Advisors, Indiana University School of Medicine, Indianapolis, IN, September 8, 1999. Preparing for Post-Tenure Review: Expanded Roles for Chairs in Faculty Evaluation & Faculty Development, with S. Hook, G. Powers, J. Dickerson-Putman, R. A. Harris, and S. Kahn, preview workshop, IUPUI, Indianapolis, IN, November 12, 1999. Role of the Department Chair in Post-Tenure Review, workshop with B. Brown and S. Barr, American Association for Higher Education, Forum on Faculty Roles & Rewards: Scholarship Reconsidered Reconsidered, New Orleans, LA, February 3, 2000. Faculty Evaluation and the Chair Role: Applications of Workload-Based Measures for Merit Pay and Post-Tenure Review, with D. Malik, "Celebrating Success: Sharing Best Practices", 17th Annual Academic Chairpersons Conference, Orlando, FL, February 9, 2000. Preparing for Post-Tenure Review: Expanded Roles for Chairs in Faculty Evaluation & Faculty Development, with W. Schneider, G. Powers, and S. Kahn, workshop, IUPUI, Indianapolis, IN, February 25, 2000. The Annual Review: The Critical Element in Faculty Evaluation and Post-Tenure Review, workshop, Winthrop University, Rock Hill, SC, April 7, 2000. 23

The Chair and New Roles in Faculty Evaluation: Workload Assignments, Career Planning, and Post-Tenure Review, workshop, University of Nebraska - Lincoln, Lincoln, NE, May, 5, 2000. The IUPUI Biology 1 Year Pre-Professional M.S.: As We Begin Year 8, Conference for Pre-medical Advisors, Indiana University School of Medicine, Indianapolis, IN, September 6, 2000. Expanded Roles for Chairs in Post-Tenure Review at IUPUI, workshop, with J. Dickerson-Putman, W. Schneider, G. Powers, S. Hook, and S. Kahn, IUPUI, Indianapolis, IN, September 29, 2000. Faculty Evaluation for Tenure and Post-Tenure Review, workshop with S. Barr and B. Brown, Columbia College, Chicago, IL, October 26, 2000. Post-Tenure Review & the Department Chair, Idaho State University, Pocatello, Idaho, November 7, 2000. Applications of Faculty Evaluation to Post-Tenure Review, workshop, with B. Brown, Virginia Military Institute, Lexington, VA, November 13, 2000. Post-Tenure Review and the Department Chair: Providing Feedback and Promoting Faculty Development, workshop with S. Barr and B. Brown, American Association for Higher Education, Forum on Faculty Roles & Rewards: The Changing Professoriate, Tampa, FL, February 1, 2001. Reducing Chair Workload and Enhancing Department Services, with L. Jenski, The 21st Century Department: New Challenges, New Solutions, 18th Annual Academic Chairpersons Conference, Orlando, FL, February 8, 2001. Preparing New Chairs, with D. Malik, The 21st Century Department: New Challenges, New Solutions, 18th Annual Academic Chairpersons Conference, Orlando, FL, February 9, 2001. Faculty Evaluation: New Applications for Chairs in Post-Tenure Review, workshop with S. Barr, The 21st Century Department: New Challenges, New Solutions, 18th Annual Academic Chairpersons Conference, Orlando, FL, February 9, 2001. Extended Roles for Chairs in Post-Tenure Review at IUPUI, workshop, with J. Dickerson-Putman, G. Powers, S. Hook, and S. Kahn, IUPUI, Indianapolis, IN, February 23, 2001. Re-visiting Faculty Evaluation, West Virginia University, Morgantown, WV, July 16, 2001. The Role of the Department Chair in Senior Faculty Development, West Virginia University, Morgantown, WV, July 16, 2001.

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Working with Post-Tenure Review: Implications for Department Chairs, workshop with S. Hook, G. Powers, and J. Lucke, IUPUI, Indianapolis, IN, November 16, 2001. Post-Tenure Review and the Department Chair: Engaging Faculty through Effective Written and Verbal Feedback, workshop with S. Barr and B. Brown, American Association for Higher Education, Forum on Faculty Roles and Rewards: Knowledge for What? The Engaged Scholar, Phoenix, AZ, January 24, 2002. Strategies for Chairs in Opening Faculty Cultures to Change, with L. Jenski, American Association for Higher Education, Forum on Faculty Roles and Rewards: Knowledge for What? The Engaged Scholar, Phoenix, AZ, January 27, 2002. Tips to the Department Chair, with D. Malik, The Changing Role of Department Chairs, 19th Annual Academic Chairpersons Conference, Orlando, FL, February 6, 2002. A Nested Mission & Goals Approach to Department Strategic Planning, with L. Jenski, 1The Changing Role of Department Chairs, 9th Annual Academic Chairpersons Conference, Orlando, FL, February 7, 2002. Round Table Discussion on Tips to Chairs, with D. Malik, The Changing Role of Department Chairs, 19th Annual Academic Chairpersons Conference, Orlando, FL, February 8, 2002. A Sampling of Best Practices from Gateway Courses: Experiments of Promise, IUPUI Town Hall Meeting on Retention, Indianapolis, IN, March 6, 2002. Off to a Good Start through Professional Development Planning, IUPUI New Faculty Orientation, panelist, Indianapolis, IN, August 15, 2002. The IUPUI Biology 1 Year Pre-Professional M.S.: Closing in on the First Decade, StateWide Conference for Pre-Medical Advisors, Indiana University School of Medicine, Indianapolis, IN, September 4, 2002. Workforce Development Initiative: Flexible Degrees in Applied Biotechnology, Indiana Biotechnology Corporations, Eli Lilly & Co., Indianapolis, IN, October 31, 2002. Isolation, Characterization, and Regulation of the Candida albicans ERG27 Gene Encoding the Sterol 3-keto Reductase, Johnson & Johnson Focused Giving Symposium, New Brunswick, NJ, December 3, 2002. Selecting New Chairs: Matching Institutional and Applicant Expectations, with L. Jenski and Mary Walczak, Visions of Departmental Leadership, 20th Annual Academic Chairpersons Conference, Orlando, FL, February 6, 2003. Chair Leadership Beyond the Department, with D. Malik, Visions of Departmental Leadership, 20th Annual Academic Chairpersons Conference, Orlando, FL, February 5, 2003.

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The Major Issues Facing Departments in the Near Future - A National Perspective, Chair Leadership Series, Office of Professional Development, IUPUI, Indianapolis, IN, February 19, 2003. Rethinking Faculty Appointments, with B. Brown, AAHE Learning to Change Conference, Good Work in Challenging Times, Washington, D. C., March 16, 2003. Playing Well with Others: A Community of Practice on Retention, with N. Chism and S. Evenbeck, AAHE Learning to Change Conference, "Good Work in Challenging Times", Washington, D. C., March 16, 2003. The Department of Biology at IUPUI and Its Relevance to the Central Indiana Life Sciences Initiative, Central Indiana Life Sciences Initiative, IUPUI, August 25, 2003. The IUPUI 1 Year Pre-Professional Master of Science: Beginning the Second Decade, Statewide Conference for Pre-Medical Advisors, Indiana University School of Medicine, Indianapolis, IN, September 3, 2003. Disruption of the Candida albicans CYB5 Gene Results in Increased Azole Sensitivity, Johnson & Johnson Focused Giving Symposium, New Brunswick, NJ, December 2, 2003. BioFuture: The IUPUI School of Science Degree Programs in Biotechnology, Biotechnology Curriculum Development Conference, IUPUI, Indianapolis, IN, December 16, 2003. Using the External Review Process Wisely: Fostering Improvement and Understanding, with L. Jenski and D. Malik, Pre-conference workshop, Choosing the Right Path among Many Options: Creating a Meaningful Niche, 21tst Annual Academic Chairpersons Conference, Orlando FL, February 3, 2004. Using Advisory Groups to Promote Department Needs and Objectives, with L. Jenski, Choosing the Right Path among Many Options: Creating a Meaningful Niche, 21tst Annual Academic Chairpersons Conference, Orlando FL, February 5, 2004. Creating External Partnerships to Enhance Department Relevance, Image, and Fiscal Stability, with D. Malik, Choosing the Right Path among Many Options: Creating a Meaningful Niche, 21tst Annual Academic Chairpersons Conference, Orlando FL, February 5, 2004. Riding the Waves of Generational Change, with L. Jenski, AAHE Learning to Change Conference, Learning in 3D, San Diego, CA, April 3, 2004. Engagement through Collaboration: Transforming the Local Economy through Innovative Academic Programming, AAHE Learning to Change Conference, Learning in 3D, San Diego, CA, April 2, 2004. Ensuring Your Own Development as a Department Chair, University of North Carolina Leadership Institute, Chapel Hill, NC, June 7, 2004. 26

The IUPUI School of Science Degree Programs in Biotechnology, Baxter Pharmaceutical Solutions, Bloomington, IN, July 22, 2004. Sterol Uptake in Candida glabrata: Implications for Antifungal Efficacy, with M. Bard, Johnson & Johnson Focused Giving Symposium, New Brunswick, NJ, November 30, 2004. Staffing Academic Departments, with B. Brown, The Chairs Role in Empowering Change, 22nd Annual Academic Chairpersons Conference, Orlando FL, February 9, 2005. Using Public Information Forums to Generate Support for New Initiatives, with L. Jenski, The Chairs Role in Empowering Change, 22nd Annual Academic Chairpersons Conference, Orlando FL, February 10, 2005. The History of Biotechnology Program Development in Central Indiana, Biotechnology Internship Development Conference, IVY Tech State College, Indianapolis, Lawrence Campus, February 17, 2005. BioFuture: The Status of Biotechnology Degree Programs at IUPUI, Biotechnology Internship Development Conference, IVY Tech State College, Indianapolis, Lawrence Campus, February 17, 2005. The Freshman Work Program: A Tool for Student Success and Retention, AAHE National Conference on Higher Education, Rallying the Trendsetters in Higher Education, Atlanta, GA, March 18, 2005. Biotechnology and Academia, with D. Stocum and K. Lee, Attain Conference, Empowerment through Employment: Bridging Perceptions Promoting Diversity, Indianapolis, IN, March 29, 2005. Creating a Community College System with the Help of Industry in Indiana: Preparing for a Scientific and Economic Revolution, with W. Lang, J. Joyce, A. Cothrel and K. Lee, BIO 2005, Community College Program, Philadelphia, PA, June 19, 2005. Roundtable on Human Capital Trends in Indianas Life Sciences Industries, sponsored by Indianapolis Private Industry Council, Inc., Thomas B. Miller & Associates Offices, Indianapolis, IN, August 31, 2005. The Biology One-Year Pre-Professional M. S., Statewide Conference for Pre-Medical Advisors, Indiana University School of Medicine, Indianapolis, IN, August 31, 2005. Chair Leadership in Campus and Department Initiatives in Student Retention, Changing Leadership: Expectations for Chairs, 23rd Annual Academic Chairpersons Conference, Orlando, FL, February 9, 2006. Faculty Evaluation: Continuing Challenges for Chairs, with C. Licata, Changing Leadership: Expectations for Chairs, 23rd Annual Academic Chairpersons Conference, Orlando, FL, February 10, 2006. 27

Managing Change, University of North Carolina Leadership Institute, Chapel Hill, NC, May 24, 2006. The Changing Roles of the Department Chair: Then, Now, and into the Future, Leadership Development: Workshop for New Department Chairs and Program Directors, IUPUI, October 27, 2006. Fostering Change: Framing Issues and Overcoming Resistance, IUPUI School of Liberal Arts, November 17, 2006 Effective Chairing in an Era of Change, Keynote Address, SOCHE (Southwestern Ohio Council for Higher Education) Academic Leaders Conference, Chairing for the Future, Wittenberg University, Springfield, OH, December 8, 2006. Fostering Your Own Development as Department Chair, facilitator/presenter at two sessions, SOCHE (Southwestern Ohio Council for Higher Education) Academic Leaders Conference, Chairing for the Future, Wittenberg University, Springfield, OH, December 8, 2006. Interacting with the Dean, with D. J. Malik, Academic Leadership: Building Responsive and Responsible Communities, 24th Annual Academic Chairpersons Conference, Orlando, FL, February 7, 2007. Academic Advising: Choosing a Model that Works for Your Department, Academic Leadership: Building Responsive and Responsible Communities, 24th Annual Academic Chairpersons Conference, Orlando, FL, February 8, 2007. The One-Year Pre-professional M. S. in Biology, State-wide Pre-Medical Advisors Conference, Indiana University School of Medicine, Indianapolis, IN, August 29, 2007. Continuing Chair Challenges: Evaluating Faculty and Facilitating Faculty Careers, preconference workshop with C. M. Licata, Defining Departmental Leadership: Engaging Academic Communities for Success, 25th Annual Academic Chairpersons Conference, Orlando, FL, February 5, 2008. Interdisciplinarity: Moving Beyond Traditional Boundaries, with G. Vemuri, Defining Departmental Leadership: Engaging Academic Communities for Success, 25th Annual Academic Chairpersons Conference, Orlando, FL, February 6, 2008. Accountability and the Role of the Department Chair, with D. J. Malik, Defining Departmental Leadership: Engaging Academic Communities for Success, 25th Annual Academic Chairpersons Conference, Orlando, FL, February 7, 2008. Faculty Recruitment: Trends, Challenges and Opportunities, with G. Vemuri, What is on the Horizon, 26th Annual Academic Chairpersons Conference, Orlando, FL, February 11, 2009.

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Advice for Department Chairs, with D. Malik and G. Vemuri, What is on the Horizon, 26th Annual Academic Chairpersons Conference, Orlando, FL, February 12, 2009. Facilitating and Enhancing Research and Scholarship, with D. J. Malik, What is on the Horizon, 26th Annual Academic Chairpersons Conference, Orlando, FL, February 12, 2009. Acing the Academic Job Search, presentation with D. Burr and S. Rhodes, IU School of Medicine Office of Postdoctoral Affairs and Preparing Future Faculty Program, Indianapolis, IN, September 29, 2009. Structuring Chair Work for Long-Term Productivity and Personal Satisfaction, A New Generation of Accountability, 27th Annual Academic Chairpersons Conference, Orlando, FL, February 11, 2010. A Conversation on Effective Chairmanship, with J. Gladden, K. Johnson, and V. John, IUPUI New Chairs Orientation, Indianapolis, IN, September 3, 2010. Planning Your Escape: Exiting the Chair Position, Transforming Higher Education: The Department Chairs Role, 28th Annual Academic Chairpersons Conference, Orlando, FL, February 11, 2011. Other Professional Activities: Interim Instructor, Independent Study Course in Contemporary Biology, 1978-1981 Consultant for Vitamins Inc., Chicago, IL, Summer 1981, Fall, 1991. Textbook Consultant, C. V. Mosby Co., 1978; Prentice Hall 1997; McGraw Hill, 2003 Interim Director of NSF sponsored course for secondary teachers, 1978 Research Grant Reviewer, Veterans Administration, 1992; USDA, 1995 Consultant for Indiana Farmer's Insurance Co., 1990 Exam Question Author, New York State Regents Exam in Microbiology, 1990 Manuscript Reviews: Lipids, 1992, 1994, J. Biomed. Biotech., 2003, J. Scholarship Teaching & Learning, 2008, 2009, Arch. Biochem. Biophys., 2008 NSF Academic Research Infrastructure Workshop, Washington, DC, February, 1995 Curriculum Consultant, University of Qatar, 1997-98 Pew Charitable Trusts Colloquium on "Evaluation of Academic Departments: A Strategy Paper for the Pew Charitable Trusts" by Jon Wergin and Judi Swingen, Washington, DC, March, 1999. Anker Publishing, book prospectus consultant, 1999. Book Prospectus Review, Jossey-Bass, 2010. Review (written) of Time Management for Department Chairs by C. K. Hansen, Jossey-Bass, 2010. Textbook Reviews Virology, Wm. C. Brown, 1992. The World of Microbes, Pommerville & Shagam, Prentice Hall, 2000. Consulting on post-tenure review, Idaho State University, 2000. 29

References: N. Douglas Lees

Dr. Edward Berbari Professor and Chair Department of Biomedical Engineering IUPUI 317-274-3728 eberbari@iupui.edu Dr. Scott Evenbeck President, New Community College, CUNY Former Dean, University College, IUPUI 212-652-2079 Scott.Evenbeck@mail.cuny.edu Dr. Christine Licata Senior Associate Provost Rochester Institute of Technology, NTID 585-475-2953 cmlnbt@rit.edu Dr. David J. Malik Executive Vice Chancellor for Academic Affairs, Indiana University Northwest; Chancellors Professor and Former Chair, Department of Chemistry, IUPUI 219-980-6966 dmalik@iupui.edu Dr. Bart Ng Acting Dean School of Science IUPUI 317-274-0625 bng@iupui.edu Dr. Simon Rhodes Professor of Cellular & Integrative Physiology and Associate Dean for Graduate Studies Indiana University School of Medicine 317-274-5273/317-278-7917 srhodes@iupui.edu Dr. David Stocum Professor of Biology and Former Dean, School of Science IUPUI 317-274-0627 dstocum@iupui.edu

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July 22, 2010 CURRICULUM VITAE NAME: Li, (Last) Jiliang (First)

EDUCATION AND TRAINING UNDERGRADUATE: M.D., Beijing Medical University, China, 1990 (Present name: Peking University Health Science Center) GRADUATE: Ph.D., Kagawa Medical University, Japan, 2000 (Present name: Kagawa University Faculty of Medicine) POST DOCTORAL: Indiana University School of Medicine, 2000-2002 ACADEMIC APPOINTMENTS 2006Assistant Professor, Department of Biology, Indiana University-Purdue University Indianapolis (IUPUI), Indianapolis, U.S.A 2002-2006 Research Assistant Professor, Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, U.S.A 2000-2002 Postdoctoral Fellow, Department of Anatomy and Cell Biology Indiana University School of Medicine, Indianapolis, U.S.A 1997-2000 Graduate Research Associate, Department of Orthopedic Surgery Kagawa Medical University, Japan 1996-1997 Research Fellow, Department of Orthopedic Surgery Kagawa Medical University, Japan 1995-1996 Attending Doctor, Department of Orthopedic Surgery Beijing Medical University, China 1993-1995 Chief Resident, Department of Orthopedic Surgery Beijing Medical University, China 1990-1993 Resident, Department of Orthopedic Surgery Beijing Medical University, China 1989-1990 Internship, Peking Union Medical College Hospital, China OTHER APPOINTMENTS AND PROFESSIONAL CONSULTANTSHIPS 2006Adjunct Assistant Professor, Department of Anatomy and Cell Biology, Indiana University School of Medicine 2006Indiana University Center for Regenerative Biology and Medicine LICENSURE AND CERTIFICATION: N/A. PROFESSIONAL ORGANIZATION: 2001Member of American Society for Bone and Mineral Research (ASBMR) 2004Member of Orthopedic Research Society (ORS) 2001Member of International Chinese Hard Tissue Society (ICHTS) 2004-2006 Member of American Society for Gravitational and Space Biology (ASGSB) 2005Member, of American Association of Anatomists (AAA)

July 22, 2010 20052008 The American Association for the Advancement of Science (AAAS) Member of International Bone & Mineral Society

HONORS AND AWARDS: 2006 AIMM/ASBMR John Haddad Young Investigator Award 2005 ORS New Investigator Recognition Awards finalist 2004 Alice L. Jee Young Investigator Award 34th International Sun Valley Workshop on Skeletal Tissue Biology, USA 2002 ASBMR Young Investigator Award American Society for Bone and Mineral Research, USA 2002 Louis V. Avioli Best Article Award, Calcified Tissue International. 2000-2002 Postdoctoral fellowship Indiana University School of Medicine, Indiana, USA 2001 Finalist in 2001 year's New Investigator Recognition Awards Orthopedic Research Society 2001 Annual Meeting, San Francisco, USA 2001 The Young Investigator Travel Award 31st International Sun Valley Hard Tissue Workshop, Idaho, USA 2001 2001 Travel Award International Chinese Hard Tissue Society, USA 2000 Nishida award (Most Outstanding Ph.D. Graduate of the year) Kagawa Medical University, Kagawa, Japan 1997-2000 Japanese Government Scholarship for Ph.D. student Kagawa Medical University, Kagawa, Japan 1996 Japanese Government Scholarship for research student Kagawa Medical University, Kagawa, Japan PROFESSIONAL SERVICE Manuscript Reviews (8/2006 present) 1. Bone - 2 times (2007) + 3 times (2009) + 5 times (2010) 2. Alcoholism: Clinical and Experimental Research - 1 time (2008) 3. Calcified Tissue International - 2 times (2008) + 3 times (2009) + 6 times (2010) 4. Indian Orthopedic journal -1 time (2008) + 1 time (2010) 5. Journal of Biomedical Science and Engineering (JBISE) 1 time (2009) 6. Osteoporosis International 1 time (2010) 7. Cell Adhesion and Migration 2 times (2010) Conference Abstract Committee 1. 2010 Orthopedic Research Society Annual Meeting a total of 58 abstracts. Ad Hoc Grant Reviewer 2. North Carolina Biotechnology Center, 2008 3. National Aeronautics and Space Administration (NASA) Experimental Program to Stimulate Competitive Research (EPSCoR) in South Carolina, 2009 UNIVERSITY SERVICE IUPUI:

July 22, 2010 1. Reviewer for the IUPUI UROP program 20072. Undergraduate Research Opportunities Program Symposium 3-28-2008 3. Project SEED Summer Research Program, Indiana Section, American Chemical Society, June 2 - July 25, 2008 4. The Diversity Scholars Research Program (Brianna Scott) 2008 Fall 5. Committee of Bowling-Jones-Russo Award 2009 6. Committee of Chancellors Award for Undergraduate Research 2009 7. IUPUI Multidisciplinary Undergraduate Research Institute, June 1 July 31, 2009 8. Bridges to the Baccalaureate Program, summer 2009 School: 1. Vivarium Committee 2008 - 2010 Departmental: 1. Graduate Cell and Developmental Biology Ph.D. Qualifying Exam Committee 2. Seminar Committee January 20083. Graduate Committees: Joseph Martin, Chair Kaarthik Chandrasekhar, Chair America Newnum, Chair Hongkang Zhou, Chair Keith Rennier (BME), Member 4. Search Committee for Faculty in Forensic Biology 2009 5. Search Committee for Faculty in Biology 2009 -2010 OTHER PROFESSIOANL ACTIVITIES INVITED LECTURES/SEMINARS (while in rank) 2007 Mechanotransduction in Bone at Peking University First Hospital, Peking University Health Science Center, Beijing, China. (6-6-2007) 2007 How bone cells response to fatigue loading? at Biomedical Engineering Seminar, Indiana University and Purdue University Indianapolis 2009 Molecular basis of mechanotransduction in living bone at Department of Cellular and Integrative Physiology, Indiana University School of Medicine (3-3-2009) COMMUNITY SERVICE 1. Hoosier science and Engineering fair 2007 2. Indiana Science Olympiad Regional Competition (2-9-2008) 3. The 2009 Indiana State Science Olympiad (3-28-2009) CONFERENCE PRESENTATIONS (while in rank) 1. Nelson MT2, Keith J2, Li B, Stocum D, Li J (2010) Electrospun nanofiberous composite scaffolds for improved stem cell Growth and Proliferation. 56th Annual Meeting of the Orthopedic Research Society, New Orleans, LA. 2. Li J (2009) Stat3 Regulates Bone Development and Load-Induced Bone Formation. IBMS Sun Valley Workshop: Musculoskeletal Biology 39th International workshop, Sun Valley, Idaho.

July 22, 2010 3. Martin JR1, Nelson MT2, Yu X, Ferries IK1, Chandrasekhar K1, Li J (2009) Osteoblast-specific inactivation of Stat3 decreases mechanical responsiveness. J Bone Miner Res. 24 (S1): S19. (Plenary poster) 4. Doyon AR2, Li J (2009) Effects of parathyroid hormone and glucocorticoid on fracture repair in mice. 23rd National Conference on Undergraduate Research (NCUR), La Crosse, Wisconsin 5. Gaddy AR2, Roper RJ, Li J (2009) Bone Development in a Down Syndrome Mouse Model. 23rd National Conference on Undergraduate Research (NCUR), La Crosse, Wisconsin. 6. Blazek J1, Gaddy A2, Meyer R2, Li J, Roper R (2009) Altered bone formation and homeostasis in the TS65DN mouse model of Down Syndrome, 23rd International Mouse Genome Conference. La Jolla, CA 7. Stepan JJ, Dobnig H, Burr DB, Li J, Ma YL, Sipos A, Petto H, Pavo I (2008) Histomorphometric Changes by Teriparatide in Alendronate Pre-treated Women with Osteoporosis. In 29th Annual Meeting of American Society for Bone and Mineral Research, Montral, Quebc, Canada 8. Li J, Doyon AR, Ferries IK (2008) Effects of Parathyroid Hormone on Fracture Repair in Glucocorticoid Treated Mice. In 29th Annual Meeting of American Society for Bone and Mineral Research, Montral, Quebc, Canada. 9. Li B, Rao N, Robling AG, Song F, Li J, Stocum D 2008 Morphological study of retinoic acid-induced malformation of skeletal tissues during axolotal limb regeneration. 38th International Sun Valley Workshop, August 3-6, 2008. 10. Li B, Rao N, Li J, Stocum D 2008 Axolotl limb regeneration: implications for bone regeneration therapies. First Midwest conference on stem cell biology & therapy May 9-11, Rochester, MI USA 11. Li B, Rao N, Li J, Stocum D 2008 Biomaterial screening for cell-baed bone regeneration therapies. Indiana Microscope Society Spring meeting, Bloomington, IN. April 18, 2008. 12. Stepan JJ, Li J, Burr DB, Michalska D, Dobnig H, Petto H, Sipos A, Pavo I 2008 Early change in a bone formation biochemical marker correlates with histomorphometric bone formation activity after 2 year teriparatide treatment in postmenapausal women with osteoporosis. European Calcified Tissue Society 34th European Symposium on Calcified Tissue. 13. Li J, Turner CH 2007 Role of the L-Type voltage-gated calcium channel Cav1.3 in bone Mechanotransduction. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii. 14. Li J, Burr DB, Stepan JJ, Dobnig H, Fahrleitner-Pammer A, Sipos A, Mullarney T, Westmore M, Sato M, Pavo I 2007 Teriparatide Improves Bone Microarchitecture in Postmenopausal Women Previously Treated with Alendronate. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii. 15. Dobnig H, Stepan JJ, Burr DB, Fahrleitner-Pammer A, Li J, Sipos A, Mullarney T, Sato M, Pavo I 2007 Teriparatide Reduces Bone Microdamage Accumulation in Postmenopausal Women Previously Treated with Alendronate. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii.

July 22, 2010 16. Pavo I, Stepan JJ, Li J, Michalska D, Sipos A, Petto H, Burr DB, Dobnig H 2007 The microdamage accumulation is more frequent in patients with impaired trabecular bone microstructure. European Calcified Tissue Society 34th European Symposium on Calcified Tissue 5/7-9/2007, Copenhagen, Denmark. 17. Bell CL, Li J, Burr DB 2006 The Role of Cav 1.3 in Mechanotransduction (Abstract # G116) in 2006 Annual Biomedical Research Conference for Minority Students (ABRCMS), 11/8-11/2006 at Anaheim, CA. 18. Li J, Li L, Simmons CR, Zhao Q, Edenberg HJ, Turner CH, Burr DB 2006 Microarray Analysis of the Molecular Profile after Mechanical Loading in Bone. In 27th Annual Meeting of American Society for Bone and Mineral Research, Philadelphia, PA. 19. Stepan JJ, Burr DB, Pavo I, Sipos A, Li J, Michalska D, Petto H, Dobnig H 2006 The correlations between serum markers and dynamic histomorphometry are more pronounced in alendronate-treated than naive patients with postmenopausal osteoporosis. In 27th Annual Meeting of American Society for Bone and Mineral Research, Philadelphia, PA. STUDENTS ADVISED Graduate Students: Hongkang Zhou, Ph.D. Candidate in Biology, 2009America Newnum, MS Candidate in Biology, 2009Keith Rennier, MS Candidate in Biomedical Engineering 2008Joseph Robert Martin, MS in Biology, 2007-2009 Kaarthik Chandrasekhar, MS in biology, 2007-2009 Undergraduate Research and Thesis (K493 K494): Anthony R. Doyon Ashley V. Sloan Muuz Zerusenay Desta Durell Lamond Dancy Manuel Carlos De La Rosa Jr. Anna Fown-Yee Leung Pfumojena C. Mushonga Briana Janel Scott Stephanie Marge Flaig Lemma G Gonfa Brittany L Eisenmenger Heli Ashish Desai Anthony Douglas Kiesel Amber Nanette Brown GRANTS AND FELLOWSHIPS IN PREPARATION 2011-2015 Stat3 Signaling in Bone. NIH 1 R01 AR057429-01 (To be re-submitted on July 2010)

July 22, 2010 2011-2013 1R03AR059943-01, Mitochondrial activity in bone metabolism. NIH Priority score: 38 (To be re-submitted on June 2010)

PENDING Principal Investigator External Grants: 2010 Chemical Genomics Approach to Discover Useful Small Molecule Compounds for Musculoskeletal Regeneration. DoD PRORP Hypothesis Development Award. 2011-2012 Cell therapy to enhance re-vascularization of allograft bone, NIH R21. Co-Investigator External Grants: 2010 NIH UO1 (PI: Theresa Guise) ONGOING Principal Investigator None. Co-Investigator External Grants: 2010-2013 Special Congressional Appropriation Regenerative Medicine for Battlefield Injuries. $1,000,000 (PI: David Stocum) 2008-2013 Connexin 43 hemichannels and signaling in bone, NIH 1R01AR05364301A2 (PI: Lilian Plotkin) 2005-2010 Mechanotransduction: Puringergic Signaling in Bone. NIH 1R01AR051901-01A1. (PI: Randall Duncan) Internal Grants: 2007-2010 IUPUI Signature Center - Biomechanics and Biomaterials Research Center, (PI: Charles Turner) 2007-2010 IUPUI Signature Center - Indiana Center for Systems Biology and Personalized Medicine, (PI: Jake Chen) Consultant External Grants: 2010-2015 Molecular Basis of Mechanotransduction in Bone Cells. NIH RO1 (PI: Jun You, PhD. The Pennsylvania State University College of Medicine) COMPLETED Principal Investigator External Grants: 2004-2007 Mechanisms of stochastic resonance on bone cells. NASA (NNA04CD04G). Total cost: $433,867.00. 2003-2005 Healing of stress fracture in an animal model (9/1/2003 9/30/2005), United States Army (DAMD17-03-1-0729). Total cost: $200,209.00.

July 22, 2010 Internal Grants: 2006-2009 IUPUI Signature Center: Center for Regenerative Biology and Medicine (PI: David L. Stocum) 2006-2009 IUPUI Signature Center: Math Bioscience Center (PI: Benzion Boukai) 2009 IUPUI Multidisciplinary Undergraduate Research Institute (MURI) Mentorship Project Proposal, $11,800. 2007-2008 Evaluation of Role of Signal Transducers and Activators of Transcription 3 in Bone Formation. IUPUI Research Support Funds Grant (RSFG). Total cost: $35,000. 2007 2007 Student Technology Funds, Request for Installation of Luminescent Image Analyzer, Total cost: $20,000. 2002-2003 IU School of Medicine Research Core Pilot Grant (7/1/02-6/30/03). Total cost: $9,600 Co-Investigator External Grants: 2005-2009 Genetic Analysis of Bone Structure and Strength. NIH 5R01AR046530-07. (PI: Charles H. Turner) 2003-2007 Remodeling Suppression: Damage Accumulation and Bone Toughness, NIH R01 AR47838 (PI: David Burr) 2002-2007 Dose response effects of residronate and alendronate on bone remodeling and microdamage accumulation, P & G Pharmaceutical. $287,155 (PI: David Burr) 2002-2006 Fatigue life of bone following treatment with anti-resorptive agents, Eli Lilly & Co. $ 22,494 (PI: David Burr) Mentor Internal Grants: 2007-2008 Rachel Meyer (BME Senior), Bone structure and mechanical properties of Down Syndrome mice. IUPUI Undergraduate Research Opportunities Program (UROP) award. 2007-2008 Anthony Doyon (Biology Junior), Parathyroid hormone (1-34) enhances fracture healing in glucocorticoid treated mice. IUPUI Undergraduate Research Opportunities Program (UROP) award SUBMITTED BUT NOT FUNDED Principal Investigator 2010-2013 1R03AR059943-01, Mitochondrial activity in bone metabolism. NIH Priority score: 38 2010-2012 Segment Defect Regeneration and custom vascularized bone grafts, Defense Advanced Research Projects Agency (DARPA) Young Faculty award 2010-2013 1R15 AR059625-01 Endothelial progenitor cells in composite nanofibers for musculoskeletal regeneration. NIH 2010-2013 Resident microvascular endothelial progenitor cells for segment defect regeneration. DoD PRORP Idea Development Award

July 22, 2010 2010-2013 2010-2013 2010 2009 2008 2008 2008 2007 Resident microvascular endothelial progenitor cells in healing of bone allografts. Musculoskeletal Transplant Foundation Electrospun Nanofibers for bone and muscle regeneration DoD Defense Medical Research and Development Program Chemical Genomics approach to discover useful small molecular compounds for bone regeneration. Pilot funding for Research use of core facilities, Indiana Clinical and Translational Sciences Institute Summer Faculty Grant from Purdue Research Foundation Stat3 Signaling in Bone. NIH 1 R01 AR057429-01 The 2009 competition for Howard Hughes Medical Institute Early Career Scientists Pew Charitable Trusts for the Pew Scholars Program in the Biomedical Sciences Role of STAT3 in Bone Adaptation, National Osteoporosis Foundation

Co-Investigator 2010-2015 The NF1 gene regulates skeletal remodeling and repair. NIH RO1 (PI: Fengchun Yang) 2010-2015 Innovative All-in-one High-performance Dental Restorative System. NIH RO1 (PI: Dong Xie) 2010-2012 Development of the Axolotl as a Research Model for Musculoskeletal Segment Defect Regeneration. NIH Challenge Grant (PI: David Stocum) EFFORTS MADE FOR IMPROVEMENT OF TEACHING, RESEARCH OR SERVICES WORKSHOP ATTENDED 1. Purdue Graduate Mentoring Workshop (10/5/2006) 2. IUPUI New Faculty Breakfast (10/6/2006) 3. Benjamin Cummings Science Workshop Strategies for Success Workshop at Indianapolis, IN (10/21/2006) 4. Faculty Grant Writing Workshop, School of Science (9/21/2006) 5. AT & T Summer Leadership Forum. June 29, 2007 6. Introduction to Oncourse CL (8/8/2007) 7. Grant writing by Robert J. Hickey (2008) 8. Scientific writing from the readers perspective (July 28/29/2008) 9. Grant writing workshop - write winning grants (11/17/2008) 10. Proposal Writing Workshop for NSF/DUE Programs, 3/6/2009 11. RO3 Writing Workshop, June 26, July 10 & 24, August 14 & 21, 2009 12. 2009 Power Up Your Pedagogy, 11/6/2009 13. IUPUI NSF Speaker Series: NSF Day AM & PM Sessions, 11/13/2009 14. NSF Career Program, once per month from1/22-2010 6/18/2010 15. NIH Grant Writing Workshop, Less is More: Practical Approaches to Writing Compelling Grant Proposals in Fewer Pages, 3/25/2010 SYMPOSIA OR MEETING ATTENDED

July 22, 2010 1. Research Retreat - Growing collaborative clinical and basic research. Indiana University School of Medicine, July 23, 2007 2. National Science Foundation Day at Butler, October 5, 2007 3. Meeting of Math Bioscience Center, October 26, 2007 4. 1st Midwest Conference on Stem Cell Biology and Therapy May 9-11, 2008 5. IUPUI 2009 Midway to Tenure workshop, October 2, 2009 OTHER ITEMS OR ENTRIES NOT ALREADY ADDRESSED 1. 2008 Promotion & Tenure Workshop (3-28-2008) 2. 2009 Promotion & Tenure Workshop (3-24-2009) RESEARCH PUBLICATIONS *** Work done at IUPUI. Written at IUPUI ** Work done both elsewhere and IUPUI. Written at IUPUI * Work revised at IUPUI. 1. *** Blazek JD, Gaddy A, Meyer RJ, Roper RJ, Li J 2010 Disruption of bone development and homeostasis by trisomy in Ts65Dn Down syndrome mice. Bone (in press) (PMID: 20870049) 2. *** Landrigan MD, Li J, Turnbull TL, Burr DB, Niebur GL, Roeder RK 2010 Contrast-enhanced micro-computed tomography of fatigue microdamage accumulation in human cortical bone. Bone (in press) (PMID: 20951850) 3. ***Van der Jagt OP, Piscaer TM, Schaden W, Li J, Kops N, Jahr H, Waarsing JH, M. de Jong V, H Weinans H 2010 Unfocused extracorporeal shock waves induce anabolic effects in rat bone. J Bone Joint Surg Am. (In press) 4. ***Stepan JJ, Burr DB, Li J, Ma YL, Petto H, Sipos A, Dobnig H, FahrleitnerPammer A, Michalska D, Pavo I. 2010 Histomorphometric Changes by Teriparatide in Alendronate Pre-treated Women with Osteoporosis. Osteoporosis International. 21(12):2027-36. (PMID: 20135094) 5. ***Doyon A, Ferries IK, Li J 2010 Glucocorticoid attenuates the anabolic effects of Parathyroid hormone on fracture repair. Calcifi Tissue Int 87:68-76. 6. ***Sloan AV, Martin JR, Li S, Li J 2010 Parathyroid hormone and bisphosphonate have opposite effects on stress fracture repair. Bone 47:235-40 7. ***Zhang Q, Carlson J, Ke HZ, Li J, Kim M, Murphy K, Mehta N, Gilligan J, Vignery A 2010 Dramatic increase in cortical thickness induced by femoral marrow ablation followed by a three-month treatment with PTH in rats. J Bone Miner Res 25(6):1350-9. 8. ***Li J, Zhao L, Ferries IK, Jiang L, Desta MZ, Yu X, Yang, Z, Duncan RL, Turner CH 2010 Sleletal phenotype of mice with a null mutation in Cav1.3 L-type calcium channel. J Musculoskelet Neuronal Interact 10(2):180-7. 9. ***Dobnig H, Stepan JJ, Burr DB, Li J, Michalska D, Sipos A, Petto H, FahrleitnerPammer A, Pavo I 2009 Teriparatide reduces bone microdamage accumulation in postmenopausal women previously treated with alendronate. J Bone Miner Res. 24(12):1998-2006. (Picture by J. Li on the cover).

July 22, 2010 10. ***Alam I, Sun Q, Koller DL, Liu L, Liu Y, Edenberg HJ, Li J, Foroud T, Turner CH 2009 Differentially expressed genes strongly correlated with femur strength in rats. Genomics. 94 (4):257-262. 11. ***Li J, Meyer RL, Duncan RL, Turner CH 2009 P2X7 Nucleotide Receptor Plays an Important Role in Callus Remodeling During Fracture Repair. Calcif Tissue Int 84(5):1227-35. 12. ***Moh A, Zhang W, Yu S, Wang J, Xu X, Li J, Fu X-Y 2008 STAT3 sensitizes insulin signaling by negatively regulating GSK-3. Diabetes. 57(5):1227-35. 13. **Liu D, Genetos DC, Shao Y, Geist DJ, Li J, Ke HZ, Turner CH, Duncan RL 2008 Activation of extracellular-signal regulated kinase (ERK1/2) by fluid shear is Ca2+ and ATP dependent in MC3T3-E1 osteoblasts. Bone. 42(4):644-52. 14. **Li J, Waugh LJ, Hui SL, Burr DB, Warden SJ 2007 Low-intensity pulsed ultrasound and nonsteroidal anti-inflammatory drugs have opposing effects during stress fracture repair. J Orthop Res. 25(12):1559-67. 15. **Stepan JJ, Burr DB, Pavo I, Sipos A, Michalska D, Li J, Fahrleitner-Pammer A, Petto H, Westmore M, Michalsky D, Sato M, Dobnig H 2007 Low bone mineral density is associated with bone microdamage accumulation in postmenopausal women with osteoporosis. Bone. 41(3):378-85. 16. **Follet H, Li J, Phipps RJ, Hui S, Condon K, Burr DB 2007 Residronate and Alendronate suppress osteocyte apoptosis following cyclic fatigue loading. Bone 40(4): 1172-1177. 17. *Sawakami K, Robling AG, Ai M, Pitner ND, Liu D, Warden SJ, Li J, Maye P, Rowe DW, Duncan RL, Warman ML, Turner CH 2006 The Wnt co-receptor LRP5 is essential for skeletal mechanotransduction but not for the anabolic bone response to parathyroid hormone treatment. J Biol Chem. 281(33):23698-711. 18. *Castillo AB, Alam I, Tanaka SM, Levenda J, Li J, Warden SJ, Turner CH. 2006 Low-amplitude, broad-frequency vibration effects on cortical bone formation in mice. Bone. 39(5):1087-96. 19. *Iwata K, Li J, Follet H, Burr DB 2006 Bisphosphonates suppress periosteal osteoblast activity independently of resorption in rat femur and tibia, Bone. 39(5):1053-8. 20. Li J, Liu D, Ke HZ, Duncan RL, Turner CH 2005 Osteogenesis after mechanical loading is linked to the P2X7 nucleotide receptor. J Biol Chem, 280(52):42952-9. 21. Li J, Miller MA, Hutchins GD, Burr DB 2005 Imaging bone microdamage in vivo with positron emission tomography. Bone 37(6):819-24. 22. Warden SJ, Hurst JA, Sanders MS, Turner CH, Burr DB, Li J 2005 Bone adaptation to a mechanical loading program significantly increased skeletal fatigue resistance. J Bone Miner Res 20(5):809-16. (TITLE ON COVER) 23. Li J, Sato M, Jerome C, Turner CH, Fan Z, Burr DB 2005 Microdamage accumulation in the monkey vertebra does not occur when bone turnover is suppressed by 50% or less with estrogen or raloxifene. J Bone Miner Metab 23S:4854. 24. Yu X, Chen S, Potter OL, Murthy SM, Li J, Pulcini JM, Ohashi N, Winata T, Everett ET, Ingram D, Clapp WD, Hock JM 2005 Neurofibromin and its inactivation of Ras are prerequisites for osteoblast functioning. Bone 36(5):793-802.

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July 22, 2010 25. Komatsubara S, Mori S, Mashiba T, Li J, Nonaka K, Kaji Y, Akiyama T, Miyamoto K, Cao Y, Kawamishi J, Norimatsu H 2004 Suppressed bone turnover by load-term bisphosphonate treatment accumulates microdamage but maintains intrinsic material properties in cortical bone f dog rib. J Bone Miner Res 19(6):999-1005. 26. Burr DB, Miller L, Grynpas M, Li J, Boyde A, Mashiba T, Hirano T, Johnston CC (2003) Tissue mineralization is increased following one-year treatment with high doses of bisphosphonates in dogs. Bone 33:960-969. 27. Li J, Duncan RL, Burr DB, Gattone VH, Turner CH 2003 Parathyroid hormone enhances mechanically induced bone formation, possibly involving L-type voltagesensitive calcium channels. Endocrinology 144:1226-1233. 28. Komatsubara S, Mori S, Mashiba T, Ito M, Li J, Kaji Y, Akiyama T, Miyamoto K, Cao Y, Kawamishi J, Norimatsu H 2003 Long-term treatment of Incadronate Disodium accumulates microdamage but improves the trabecular bone microarchitecture in dog vertebra. J Bone Miner Res 18:512-520. 29. Robling AG, Li J, Shultz KL, Beamer WG, Turner CH 2003 Evidence for a skeletal mechanosensitivity gene on mouse Chromosome 4. FASEB 17(2):324-326. 30. Tanaka SM. Li J, Duncan RL, Yokota H, Burr DB, Turner CH 2003 Effects of broad frequency vibration on cultured osteoblast. J Biomechanics 36:73-80. 31. Li J, Duncan RL, Burr DB, Turner CH 2002 L-Type calcium channels mediate mechanically induced bone adaptation in vivo. J Bone Miner Res 17:1795-1800. 32. Li J, Burr DB, Turner CH 2002 Suppression of prostaglandin synthesis with NS-398 has different effects on endocortical and periosteal bone formation induced by mechanical loading. Calcif Tissue Int 70:320-329. 33. Li J, Mashiba T, Burr DB 2001 Bisphosphonate treatment suppresses not only stochastic remodeling but also the targeted repair of microdamage. Calcif Tissue Int 69:281-286. 34. Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, and Norimatsu H 2001 Longterm effect of Incadronate Disodium (YM-175) on fracture healing of femoral shaft in growing rats. J Bone Miner Res 16:429-436. 35. Li J, Mori S, Kaji Y, Kawanishi J, Akiyama T, Norimatsu H 2000 Concentration of bisphosphonate (Incadronate) in callus area and its effects on fracture healing in rats. J Bone Miner Res 15:2042-2051. 36. Akeda M, Ueno K, Mori S, Kaji Y, Li J, Kawanishi J, Akiyama T, Li C, Norimatsu, H 2000 Histomorphometric evaluation after a single administration of incadronate disodium in rat proximal tibia. J Jpn Soc Bone Morphom 10(2):1-5. 37. Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H 1999 Effects of bisphosphonate (YM175) on fracture healing of long bone in rats. J Bone Miner Res 14:969-979. 38. Li J, Mori S, Mashiba T, Kaji Y, Taki M, Komatsubara S, Kawanishi J, Norimatsu H 1998 Preadministration of Incadronate Disodium can prevent bone loss in rat proximal tibial metaphysis when induced by hindlimb immobilization by bandage. Bone 23:459-463. 39. Kaji Y, Mori S, Kawasaki K, Li J, Li C, Kawanishi J, Akiyama T, Norimatsu H 1998 Changes in osteoclasts after a single administration of incadronate disodium. J Jpn Soc Bone Morphom 8:151-157.

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July 22, 2010 40. Kawanishi J, Mori S, Tsuji S, Kawasaki K, Li J, Kaji Y, Akiyama T, Norimatsu H 1998 Comparison of bone mineral density of calcaneus measured by three different ultrasound machines. Osteoporosis Japan 6: 183(351)-184(352). 41. Norimatsu H, Mori H, Kawanishi J, Kaji Y, Li J 1997 Immobilization as the pathogenesis of osteoporosis: experimental and clinical studies." Osteoporosis Int 7(Suppl.3): S57-S62. 42. Fang Z, Li J, Shi X 1997 Malignant soft tissue fibrous histocytoma. Chinese J Practical Surg 6:329-331. 43. Kaji Y, Mori S, Mashiba T, Kawasaki K, Li J, Norimatsu H, Nonaka K 1996 Evaluation of bone mineral densities by DXA and pQCT after short term administration of Incadronate Disodium. J Jpn Soc Bone Morphom 6:253-257. 44. Taki M, Mori S, Mashiba T, Komatsubara S, Ohtsuka I, Li J, Kawanishi J, Kaji Y, Norimatsu H, Nonaka K 1996 Prevention of osteopenia in hindlimb immobilized rats with bandage by preadministration of Incadronate Disodium. J Jpn Soc Bone Morphom 6:205-211. 45. Otsuka I, Mori S, Mashiba T, Komatsubara S, Taki M, Li J, Kawanishi J, Kaji Y, Norimatsu H, Nonaka K 1996 Prevention of osteopenia in ovariectomized rats by preadministration of Incadronate Disodium. J Jpn Soc Bone Morphom 6:221-227. 46. Komatsubara S, Mori S, Mashiba T, Ohtsuka I, Taki M, Kawanishi J, Kaji Y, Li J, Norimatsu H, Nonaka K 1996 Prevention of osteopenia in sciatic neurectomized rats by preadministration of Incadronate Disodium. J Jpn Soc Bone Morphom 6:213-220. 47. Li, J., Ma, Z., Fang, Z. 1996 Synovial chondromatosis of sternoclavicular articulation. Chinese J Orthopedics 16:790. 48. Li H, Ma Z, Li J, Shi X 1996 The plasma concentration of high-dose Methotrexate chemotherapy for patients with osteosarcoma. Chinese J Orthopedics 16:305-308. 49. Ma Z, Li H, Gao S, Mi C, Fan Z, Li J, Shi X 1995 Limb-salvage surgery in 60 patients with osteosarcoma. Chinese J Surg 33(2): 82-85. BOOK CHAPTERS Mori S, Li J, Kawaguchi Y 2000 The histological appearance of stress fracture (Chapter 10). In Burr DB, Milgram C (eds) Musculoskeletal fatigue and stress fractures. CRC press, Boca Raton, FL, USA, p 149-159 ABSTRACTS (while in rank out of a total of 83 abstracts) 1. Nelson MT2, Keith J2, Li B, Stocum D, Li J (2010) Electrospun nanofiberous composite scaffolds for improved stem cell Growth and Proliferation. 56th Annual Meeting of the Orthopedic Research Society, New Orleans, LA. 2. Li J (2009) Stat3 Regulates Bone Development and Load-Induced Bone Formation. IBMS Sun Valley Workshop: Musculoskeletal Biology 39th International workshop, Sun Valley, Idaho 3. Martin JR1, Nelson MT2, Yu X, Ferries IK1, Chandrasekhar K1, Li J (2009) Osteoblast-specific inactivation of Stat3 decreases mechanical responsiveness. J Bone Miner Res. 24 (S1): S19. (Plenary poster) 4. Doyon AR, Li J (2009) Effects of parathyroid hormone and glucocorticoid on fracture repair in mice. 23rd National Conference on Undergraduate Research (NCUR), La Crosse, Wisconsin

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July 22, 2010 5. Gaddy AR, Roper RJ, Li J (2009) Bone Development in a Down Syndrome Mouse Model. 23rd National Conference on Undergraduate Research (NCUR), La Crosse, Wisconsin. 6. Blazek J, Gaddy A, Meyer R, Li J, Roper R (2009) Altered bone formation and homeostasis in the TS65DN mouse model of Down Syndrome, 23rd International Mouse Genome Conference. La Jolla, CA 7. Stepan JJ, Dobnig H, Burr DB, Li J, Ma YL, Sipos A, Petto H, Pavo I (2008) Histomorphometric Changes by Teriparatide in Alendronate Pre-treated Women with Osteoporosis. In 29th Annual Meeting of American Society for Bone and Mineral Research, Montral, Quebc, Canada J Bone Miner Res. 22 (S1): S6. 8. Li J, Doyon AR, Ferries IK (2008) Effects of Parathyroid Hormone on Fracture Repair in Glucocorticoid Treated Mice. In 29th Annual Meeting of American Society for Bone and Mineral Research, Montral, Quebc, Canada. J Bone Miner Res. 22 (S1): S19. 9. Li B, Rao N, Robling AG, Song F, Li J, Stocum D 2008 Morphological study of retinoic acid-induced malformation of skeletal tissues during axolotal limb regeneration. 38th International Sun Valley Workshop, August 3-6, 2008. J Musculoskelet Neuronal Interact 2008; 8(4):359. 10. Li B, Rao N, Li J, Stocum D 2008 Axolotl limb regeneration: implications for bone regeneration therapies. First Midwest conference on stem cell biology & therapy May 9-11, Rochester, MI USA 11. Li B, Rao N, Li J, Stocum D 2008 Biomaterial screening for cell-baed bone regeneration therapies. Indiana Microscope Society Spring meeting, Bloomington, IN. April 18, 2008. 12. Stepan JJ, Li J, Burr DB, Michalska D, Dobnig H, Petto H, Sipos A, Pavo I 2008 Early change in a bone formation biochemical marker correlates with histomorphometric bone formation activity after 2 year teriparatide treatment in postmenapausal women with osteoporosis. European Calcified Tissue Society 34th European Symposium on Calcified Tissue. 13. Li J, Turner CH 2007 Role of the L-Type voltage-gated calcium channel Cav1.3 in bone Mechanotransduction. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii. J Bone Miner Res 21(S1):S489. 14. Li J, Burr DB, Stepan JJ, Dobnig H, Fahrleitner-Pammer A, Sipos A, Mullarney T, Westmore M, Sato M, Pavo I 2007 Teriparatide Improves Bone Microarchitecture in Postmenopausal Women Previously Treated with Alendronate. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii. J Bone Miner Res 21(S1):S28. 15. Dobnig H, Stepan JJ, Burr DB, Fahrleitner-Pammer A, Li J, Sipos A, Mullarney T, Sato M, Pavo I 2007 Teriparatide Reduces Bone Microdamage Accumulation in Postmenopausal Women Previously Treated with Alendronate. In 28th Annual Meeting of American Society for Bone and Mineral Research, Honolulu, Hawaii. J Bone Miner Res 21(S1):S28. 16. Pavo I, Stepan JJ, Li J, Michalska D, Sipos A, Petto H, Burr DB, Dobnig H 2007 The microdamage accumulation is more frequent in patients with impaired trabecular bone microstructure. European Calcified Tissue Society 34th European Symposium on Calcified Tissue 5/7-9/2007, Copenhagen, Denmark.

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July 22, 2010 17. Bell CL, Li J, Burr DB 2006 The Role of Cav 1.3 in Mechanotransduction (Abstract # G116) in 2006 Annual Biomedical Research Conference for Minority Students (ABRCMS), 11/8-11/2006 at Anaheim, CA. 18. Li J, Li L, Simmons CR, Zhao Q, Edenberg HJ, Turner CH, Burr DB 2006 Microarray Analysis of the Molecular Profile after Mechanical Loading in Bone. In 27th Annual Meeting of American Society for Bone and Mineral Research, Philadelphia, PA. 20. Stepan JJ, Burr DB, Pavo I, Sipos A, Li J, Michalska D, Petto H, Dobnig H 2006 The correlations between serum markers and dynamic histomorphometry are more pronounced in alendronate-treated than naive patients with postmenopausal osteoporosis. In 27th Annual Meeting of American Society for Bone and Mineral Research, Philadelphia, PA. 21. Li J 2006 ATP, P2 receptors and L-type calcium channels in the response of bone to loading. In 2006 Advances in mineral metabolism AIMM/ASBMR John Haddad young investigators meeting. Snowmass, CO. 4/10-15/2006. 22. Li J, Warden SJ, Waugh L, Burr DB 2006 Effect of low intensity pulsed untrasound and a cyclooxygenase-2 inhibitor on stress fracture repair. Trans Orthop Res Soc 31: Paper No: 0311; In 52nd annual meeting of Orthopaedic Research Society, Chicago 3/19-22/06. 23. Li J, Stockwell SW, Simmons CR 2006 Activation of Extracellular-Signal Regulated Kinase (ERK1/2) by stochastic resonance is Ca2+ dependent in MC3T3E1 osteoblasts. Experimental Biology meeting, San Francisco, April 1-4, 2006. 24. Stepan JJ, Burr DB, Pavo I, Sipos A, Michalska D, Li J, Petto H, Westmore M, Michalsky D, Sato M, Dobnig H 2005 Prevalent fractures, low bone mineral density and ageing are associated with greater histomorphometric indicators of microdamage accumulation in postmenopausal women. In 26th Annual Meeting of American Society for Bone and Mineral Research, Nashville, Tennessee. 25. Turner CH, Alam I, Sun Q, Li J, Fuchs RK, Edenberg HJ, Koller DL, Foroud T, Econs MJ 2005 The leptin receptor strongly influences bone structure and strength: results from a genome-wide screen in rats. In 26th Annual Meeting of American Society for Bone and Mineral Research, Nashville, Tennessee. 26. Warden SJ, Hurst JA, Sanders MS, Turner CH, Burr DB, Li J 2005 Exerciseinduced Bone Adaptation Significantly Increases Skeletal Fatigue Resistance. Medicine & Science in Sports & Exercise 37(5): S452, 2005 27. Warden SJ, Hurst JA, Sanders MS, Turner CH, Burr DB, Li J 2005 Bone adaptation to a mechanical loading program significantly increases skeletal fatigue resistance. Trans Orthop Res Soc 30: Paper No: 0652; In 51st annual meeting of Orthopaedic Research Society, Washington D. C. 2/20-23/05. 28. Li J, Miller MA, Hutchins GD, Burr DB 2005 Imaging bone microdamage in vivo with positron emission tomography. Trans Orthop Res Soc 30: Paper No: 0033; In 51st annual meeting of Orthopaedic Research Society, Washington D. C. 2/20-23/05. 29. Li J, Liu D, Ke HZ, Duncan RL, Turner CH 2005 P2X7 nucleotide receptor mediates the osteogenic response to mechanical loading through prostaglandin E2. Trans Orthop Res Soc 30: Paper No: 0379; In 51st annual meeting of Orthopaedic Research Society, Washington D. C. 2/20-23/05.

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July 22, 2010 30. Follet H, Li J, Burr DB 2005 Both residronate and Alendronate significantly reduce osteocyte apoptosis. Trans Orthop Res Soc 30: Paper No: 1544; In 51st annual meeting of Orthopaedic Research Society, Washington D. C. 2/20-23/05. 31. Sawakami K, Robling AG, Pitner ND, Warden SJ, Li J, Ai M, Warman ML, Turner CH 2005 Site-specific osteopenia and decreased mechanoreactivity in Lrp5-Mutant mice. Trans Orthop Res Soc 30: Paper No: 0386; In 51st annual meeting of Orthopaedic Research Society, Washington D. C. 2/20-23/05. 32. Li J, Liu D, Ke HZ, Duncan RL, Turner CH 2004 Osteogenesis after mechanical loading requires the P2X7 nucleotide receptor. In 25th Annual Meeting of American Society for Bone and Mineral Research, Seattle, Washington. 33. Liu D, Li J, Turner CH, Duncan RL 2004 P2X7 Purinergic receptor activation modulates prostaglandin synthesis and release from osteoblasts in response to fluid shear. In 25th Annual Meeting of American Society for Bone and Mineral Research, Seattle, Washington. 34. Sawakami K, Robling AG, Pitner ND, Warden SJ, Li J, Ai M, Warman ML, Turner CH 2004 Site-specific osteopenia and decreased mechanoreactivity in Lrp5-Mutant mice. In 25th Annual Meeting of American Society for Bone and Mineral Research, Seattle, Washington. 35. Li J, Burr D 2004 High-resolution PET can image bone microdamage in vivo. In 34th Sun Valley Workshop on Skeletal Tissue Biology, August 1-4, 2004. 36. Li J, Burr D, Fan Z, Rho JY, Jerome C, Sato M, Turner CH 2003 Raloxifene and Estrogen Do Not Affect Microdamage Accumulation or State of Mineralization in Monkey Vertebra. J Bone Miner Res 18(suppl 1): S164; In 24th Annual Meeting of American Society for Bone and Mineral Research, Minneapolis, MN, USA. 9/19-23/03. 37. Li J, Duncan RL, Burr DB, Turner CH 2002 Parathyroid hormone enhances mechanically induced bone formation through activation of L-type voltagesensitive calcium channels. J Bone Miner Res 17(suppl 1): S191; In 24th Annual Meeting of American Society for Bone and Mineral Research, San Antonio, Texas, USA. 9/20-24/02. (ASBMR Young Investigator Award) 38. Li J, Burr DB, Duncan RL, Turner CH 2002 Calcium channels mediate mechanically induced bone adaptation in vivo. Trans Orthop Res Soc 27: Paper No: 0041; In 48th annual meeting of Orthopaedic Research Society, Dallas, TX, USA. 2/10-13/02. 39. Tanaka SM, Li J, Duncan RL, Burr DB, Yokota H, Turner CH 2002 Mechanical vibration stimulates osteoblasts cultured in collagen gel. Trans Orthop Res Soc 27: Poster No:0534; In the 48th annual meeting of Orthopaedic Research Society, Dallas, TX, USA. 2/10-13/02. 40. Li J, Norvell SM, Ponik SM, Pavalko FM, Burr DB, Turner CH 2001 Relationship Between Prostaglandins and Cyclooxygenase-2 in Response to Mechanical Stimuli and Their Role in Load-induced Bone Formation. J Bone Miner Res 16(suppl 1): S482; In 23rd Annual Meeting of American Society for Bone and Mineral Research, Phoenix, Arizona, USA. 10/12-16/01. 41. Tanaka SM, Li J, Duncan RL, Burr DB, Turner CH 2001 Mechanical stimulation with broad frequency vibration promotes differentiation of osteoblasts in 3Dculture. J Bone Miner Res 16(suppl 1): S481; In 23rd Annual Meeting of

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July 22, 2010 American Society for Bone and Mineral Research, Phoenix, Arizona, USA. 10/12-16/01. 42. Li J, Duncan RL, Burr DB, Turner CH 2001 L-Type Calcium Channels Mediate Mechanically Induced Bone Adaptation in vivo. Journal of Musculoskeletal & Neuronal Interactions 2(1): 98-99; In 31st International Sun Valley Hard Tissue Workshop, Sun Valley, Idaho, USA. 8/6-10/01. (The Young Investigator Travel Award; 2001 Travel Award) 43. Li J, Mashiba T, Burr DB, 2001 Bisphosphonate treatment suppresses targeted repair of microdamage. Trans Orthop Res Soc 26: 0320; In the 47th annual meeting of Orthopaedic Research Society, San Francisco, USA. 2/25-28/01. (Semi-Final in 2001 year's New Investigator Recognition Awards) 44. Li J, Mori S, Kaji Y, Akiyama T, Norimatsu H 2000 Microdamage accumulation and biomechanical properties of dog rib after 3 years bisphosphonate treatment. J Bone Miner Res 15 (suppl 1): S227 & S308; In 22nd Annual Meeting of American Society for Bone and Mineral Research, Toronto, Canada. 9/22-26/00. (Plenary Poster) 45. Kawaguchi Y, Mori S, Hasegawa T, Oka S, Li J, Norimatsu H 2000 Pathological characteristics and gene expression at fatigue fracture in vivo. The 15th Annual Orthopedic Research Meeting of the Japanese Orthopedic Association. Kyoto, Japan. 9/28-29/00. 46. Mori S, Li J, Li C 2000 Effect of suppression of bone resorption on fracture healing. Journal of Japanese association of mechanics 99-37: 89-90. In The 12th bioengineering conference Kanezawa, Japan, 1/11-12/00. 47. Ueno K, Yamori T, Mori S, Kaji Y, Li J, Akiyama T, Norimatsu H 2000 Prevention of bone loss by a single administration of incadronate disodium in OVX rats. The 20th Meeting of Japanese Society of Bone Morphometry. Nagasaki, Japan 6/23-24/00. 48. Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Li C, Norimatsu H 1999 Effects and concentration of bisphosphonate in bone during fracture healing of rat's femur. The 14th Annual Orthopedic Research Meeting of the Japanese Orthopedic Association. Nara, Japan. 10/7-8/99. 49. Mori S, Li J, Kaji Y, Akiyama T, Li C, Norimatsu H 1999 Bisphosphonate effects and concentration in bone during fracture healing of rat's femur under Incadronate treatment. In 21st Annual Meeting of American Society of Bone and Mineral Research, St. Louis, USA. 9/30-10/4/99. 50. Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H 1999 Preadministration of Incadrnate disodium can prevent bone loss in rat's proximal tibiae induced by ovariectomy at 8 weeks after administration. In 17th annual meeting of Japanese Society of Bone and Mineral Metabolism, Osaka, Japan. 7/29-31/99. 51. Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H 1999 Long term effects of Incadronate disodium (YM-175) on fracture healing of rat's femur. The 19th Meeting of Japanese Society of Bone Morphometry, Kitakyushou, Japan, 6/25-26/99 52. Ueno K, Akeda M, Mori S, Kaji Y, Li J, Kawanishi J, Akiyama T, Li C, Norimatsu H 1999 Concentration of Incadronate disodium in rat tibia after a

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July 22, 2010 single administration. The 19th Meeting of Japanese Society of Bone Morphometry, Kitakyushou, Japan, 6/25-26/99 53. Li J, Mori S, Kaji Y, Kawanishi J, Li C, Akiyama T, Norimatsu H 1999 Effects of Bisphosphonate on early callus formation of fracture healing in rats. In The 3rd International Congress on Osteoporosis, Xi'an, China, 3/31-4/4/99. 54. Li C, Mori S, Li J, Kaji Y, Akiyama T, Kawanishi J, Norimatsu H 1999 Long term effects of Incadronate disodium (YM-175) on fracture healing of rat's femur. In The 3rd International Congress on Osteoporosis, Xi'an, China, 3/31-4/4/99. 55. Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Akiyama T, Li C, Norimatsu H 1999 Fracture healing of long bone under bisphosphonate (YM175) treatment in rats. In the 45th annual meeting of Orthopaedic Research Society, Anaheim, CA. USA. 2/1-4/99. 56. Li J, Mori S, Mashiba T, Taki M, Kaji Y, Kawanishi J, Komatsubara S, Norimatsu H 1998 Short-term administration of Incadronate Disodium(YM-175) can prevent osteopenia on rat's tibial metaphysis induced by hindlimb immobilization. In the 3rd Combined Meeting of the Orthopaedic Researach Societies, Hamamatsu, Japan. 9/28-30/98. 57. Li J, Mori S, Kaji Y, Kawanishi J, Akiyama T, Ueno K, Akeda M, Li C, Norimatsu H 1998 Effect YM-175 on callus formation during fracture healing in rats. J Jpn Soc Bone Miner Res 16(2):298; In 16th annual meeting of Japanese Society of Bone and Mineral Metabolism, Tokyo, Japan. 8/ 5-8/98. 58. Akiyama T, Mori S, Kawanishi J, Kaji Y, Li J, Li C, Ueno K, Norimatsu H 1998 Effect of pre-treatment with bisphosphonate on steroid-induced osteoporosis. J Jpn Soc Bone Miner Res 16(2):299; In the 16th annual meeting of Japanese Society of Bone and Mineral Metabolism, Tokyo, Japan. 8/5-8/98. 59. Kawanishi J, Mori S, Li J, Kaji Y, Akiyama T, Norimatsu H 1998 Factors affect bone mineral density of calcaneus in dialysis patients. J Jpn Soc Bone Miner Res 16(2):328; In 16th annual meeting of Japanese Society of Bone and Mineral Metabolism, Tokyo, Japan. 8/5-8/98. 60. Akeda M, Ueno K, Mori S, Kaji Y, Li J, Kawanishi J, Akiyama T, Kawasaki K, Norimatsu H 1999 Histomorphometric evaluation after a single administration of incadronate disodium in rat proximal tibia. J Jpn Soc Bone Morphom 8:S29; In the 18th Meeting of Japanese Society of Bone Morphometry, Okayama, Japan, 6/26-27/98 61. Akeda M, Kaji Y, Mori S, Li J, Norimatsu H 1998 Histomorphometric evaluation after single administration of Incadronate disodum in rat proximal tibia. J Bone Miner Metab 16:55. 62. Norimatsu H, Mori S, Kawanishi J, Kaji Y, Li J 1997 Immobilization as the Pathogenesis of Osteoporosis (Experimental and Clinical Studies). Osteoporosis Int 7(Suppl 2):26; In the 2nd International Conference on Osteoporosis, Osaka, Japan. 11/13-16/97. 63. Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Akiyama T, Norimatsu H 1997 Effects of Bisphosphonate on Fracture Healing of Rat's Femur. Osteoporosis Int 7(Suppl 2):39; In the 2nd International Conference on Osteoporosis, Osaka, Japan. 11/13-16/97.

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July 22, 2010 64. Norimatsu H, Mori S, Kawanishi J, Kaji Y, Li J 1997 Bone changes during immobilization and mobilization period after arthropplastic surgery. Osteoporosis Int 7(Suppl 2):69; In the 2nd International Conference on Osteoporosis, Osaka, Japan. 11/13-16/97. 65. Li J, Mori S, Kaji Y, Norimatsu H 1997 Effects of bisphosphonate on fracture healing in rats. Cent Jpn Orthop Trauma 40:67; In the 89th annual meeting of the Central Japan Association of Orthopaedic Surgery & Traumatology, Kobe, Japan, 10/9-10/97. 66. Kaji Y, Mori S, Kawasaki K, Mashiba T, Li J, Norimatsu H 1997 Single Administration of Incadronate Disodium increases osteoclast number in vivo in mice. J Bone Miner Res 12(1): S423; In the 19th Annual Meeting of the American Society for Bone and Mineral Research, Cincinnati, OH, USA. 9/10-14/97, abstr. S281 67. Mori S, Li J, Kaji Y, Mashiba T, Kawasaki K, Kawanishi J, Norimatsu H 1997 Effect of Incadronate Disodium on fracture heasling of rat's femur. In 28th International Sun Valley Workshop on Hard Tissue Biology, Idaho, U.S.A., 8/11/11-16/97. abst. p4. 68. Li J, Mori S, Kaji Y, Mashiba T, Kawanishi J, Norimatsu H 1997 Effect of YM175 on Fracture Healing in Rats: Evaluation by Histomorphometric Analysis. J Jpn Soc Bone Miner Res 15(2):211; In the 15th Meeting of Japanese Association for Bone and Mineral Metabolism, Omiya, Japan, 7/24-26/97. 69. Kawanishi J, Mori S, Mashiba T, Kawasaki K, Li J, Kaji Y, Norimatsu H 1997 Osteoporosis in adult screened by ultrasound measurement. J Jpn Soc Bone Miner Res 15(2):279; In the 15th Meeting of Japanese Association for Bone and Mineral Metabolism, Omiya, Japan, 7/24-26/97. 70. Kaji Y, Mori S, Kawasaki K, Li J, Kawanishi J, Norimatsu H 1997 Time-course effects of pretreatment of Incadronate Disodium on differentiation and proliferation of osteoblast and osteoclast in mice. J Jpn Soc Bone Miner Res 15(2):284; In the 15th Meeting of Japanese Association for Bone and Mineral Metabolism, Omiya, Japan, 7/24-26/97. 71. Kawanishi J, Mori S, Tsuji S, Kawasaki K, Li J, Kaji Y, Norimatsu H 1997 Comparison of bone mineral density of calcaneus measured by different ultrasound machines. J Jpn Soc Bone Morphom 7:S14; In the 17th Meeting of Japanese Society of Bone Morphometry, Takamatsu, Japan, 7/4-5/97. 72. Kaji Y, Mori S, Kawasaki K, Mashiba T, Li J, Kawanishi J, Norimatsu H 1997 Time-course changes in osteoclasts after a single administration of incadronate disodium in mice. J Jpn Soc Bone Morphom 7:S72; In the 17th Meeting of Japanese Society of Bone Morphometry, Takamatsu, Japan, 7/4-5/97. 73. Komatsubara S, Mori S, Mashiba T, Kaji Y, Kawanishi J, Li J, Ohtsuka I, Norimatsu H 1997 Preadministration of incadronate disodium can prevent proximal tibia bone loss in rats with hindlimb immobilization induced by sciatic neurectomy. J Bone Miner Metab. 15: 42, 97. In the 6th Chugoku meeting for bone and mineral metabolism, Okayama, Japan, June 1996. 74. Li J, Mori S, Kaji Y, Kawanishi J, Taki M, Komatsubara S, Otsuka I, Norimatsu H 1997 Preadmnistration of Incadronate disoduim can prevent prosximal tibia bone loss in rats with banadage-induced hindlimb immobilization. J Bone Miner

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July 22, 2010 Metab. 15: 42-43, 1997. In the 6th Chugoku meeting for bone and mineral metabolism, Okayama, Japan, June 1996. 75. Kaji Y, Mori S, Kawanishi J, Mashiba T, Kawasaki K, Li J, Akeda M, Norimatsu H 1997 Severe spinal osteoporosis with myelodysplastic syndrome. J Bone Miner Metab 15:48. In the 6th Chugoku meeting for bone and mineral metabolism, Okayama, Japan, June 1996. 76. Li J, et al. "Effect of YM-175 on Fracture Healing in Rats: Evaluation by X-ray and Biomechanics" J Jpn Orthop Assoc 70(8):S1498; In the 11th Annual Orthopedic Research Meeting of the Japanese Orthopedic Association. Kagoshima, Japan. 10/17-18/1996. 77. Kaji Y, Mori S, Mashiba T, Li J, Kawanishi J, Taki M, Komatsubara S, Ohtuka I, Norimatsu H 1996 Time-course of bone changes after short-term treatment with incadronate disodium in rats. J Jpn Orthop Assoc 70(8):S1236; In the 11th Annual Orthopedic Research Meeting of the Japanese Orthopedic Association. Kagoshima, Japan. 10/17-18/1996. 78. Kaji Y, Mori S, Mashiba T, Li J, Otsuka I, Komatsubara S, Kawanishi J, Norimatsu H 1996 Bisphosphonate treatment induces sclerotic band in long bones in rats. J Jpn Soc Bone Morphom 6:145; In the 16th Meeting of Japanese Society of Bone Morphometry. Niigata, Japan. 8/29-30/1996. 79. Komatsubara S, Mori S, Mashiba T, Otsuka I, Taki M, Kawanishi J, Kaji Y, Li J, Norimatsu H 1996 Prevention of osteopenia in sciatic neurectomized rats by preadministration of Incadronate Disodium. J Jpn Soc Bone Morphom 6:146; In the 16th Meeting of Japanese Society of Bone Morphometry. Niigata, Japan. 8/29-30/1996. 80. Mori S, Mashiba T, Kaji Y, Li J, Kawanishi J, Komatsubara S, Otsuka I, Norimatsu, H 1996 Effects of bisphosphonate on fracture healing. J Jpn Soc Bone Morphom 6:147; In the 16th Meeting of Japanese Society of Bone Morphometry. Niigata, Japan. 8/29-30/1996. 81. Mashiba T, Mori S, Kawanishi J, Kaji Y, Li J, Norimatsu H 1996 X-ray characteristics of proximal femur in patients with fractures of femur necks. J Jpn Soc Bone Morphom 6:181; In the 16th Meeting of Japanese Society of Bone Morphometry. Niigata, Japan. 8/29-30/1996. 82. Li J, Mori S, Mashiba T, Kaji Y, Kawanishi J, Taki M, Komatsubara S, Otsuka I, Norimatsu H 1996 Short-term Preadministration of Incadronate Disodium can prevent bone loss of Proximal Tibia of Hindlimb Immobilized Rats by Bandage. J Jpn Soc Bone Morphom 6:196; In the 16th Meeting of Japanese Society of Bone Morphometry. Niigata, Japan. 8/29-30/1996. 83. Mori S, Li J, Kaji Y, Mashiba Y, Otsuka I, Komatsubara S, Norimatsu H, Nonaka K 1996 Effects of bisphosophonates on fracture healing of rat's femur. Bone 19(3);154; In 7th Bone Morphometry Congress, Italy, 10/6-10/1996. 84. Kawanishi J, Mori S, Mashiba T, Kaji Y, Li J, Norimatsu H 1996 Risk factors related to compressive vertebral fractures in adult. J Jpn Soc Bone Miner Res 14(2):89; In the 14th Meeting of Japanese Association for Bone and Mineral Metabolism, Osaka, Japan, 7/18-20, 1996. 85. Komatsubara S, Mori S, Mashiba T, Otsuka I, Taki M, Kawanishi J, Kaji Y. Li J, Norimatsu H 1996 Comparison of osteopenia induced by bandage immobilization

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July 22, 2010 and sciatic neurectomy in rats. J Jpn Soc Bone Miner Res 14(2):260; In the 14th Meeting of Japanese Association for Bone and Mineral Metabolism, Osaka, Japan, 7/18-20, 1996. 86. Kawanishi J, Mori S, Mashiba T, Kaji Y, Li J, Norimatsu H 1996 Osteoporotic fractures in male. J Jpn Soc Bone Miner Res 14(2):266; In the 14th Meeting of Japanese Association for Bone and Mineral Metabolism, Osaka, Japan, 7/18-20, 1996. 87. Mashiba T, Mori S, Kaji Y, Kawasaki K, Kawanishi J, Li J, Akeda M, Norimatsu H 1996 Lasting effect of bisphosphonate on bone resorption after short-term treatment in rats. J Jpn Soc Bone Miner Res 14(2):278; In the 14th Meeting of Japanese Association for Bone and Mineral Metabolism, Osaka, Japan, 7/18-20, 1996. 88. Kaji Y, Mori S, Mashiba T, Kawasaki K, Kawanishi J, Li J, Funayama M, Norimatsu H 1996 Time-course analysis after YM-175 treatment in rat bones. J Jpn Soc Bone Miner Res 14(2):338; In the 14th Meeting of Japanese Association for Bone and Mineral Metabolism, Osaka, Japan, 7/18-20/1996. TEACHING Lecture Courses Course # Course Title BME 352 TISSUE BEHAVIOR & PROP BIOL 697 MOLECULAR & CELL BONE BIOLOGY BME 352 TISSUE BEHAVIOR & PROP BIOL 697 MOLECULAR & CELL BONE BIOLOGY BME 352 TISSUE BEHAVIOR & PROP BIOL 69700 MOLECULAR & CELL BONE BIOLOGY BME 35200 TISSUE BEHAVIOR & PROP

Term 2007 Spring 2007 Fall 2008 Spring 2008 Fall 2009 Spring 2009 Fall 2010 Spring

N 13 31 16 34 12 31 33

Credits Global scores 3 3 3 3 3 3 3 4.33 4.37 4.28 3.52 4.55

Individualized Instruction Course# Students 698 K493 K493 K493 698 698

Year Credit Enrollment Hours Spring Fall Summer Martin, Joseph Robert 2007 6 X Desta, Muuz Zerusenay 2007 1 X Doyon, Anthony Robert 2007 1 X Sloan, Ashley Vaden 2007 1 X Martin, Joseph Robert 2008 9 X X Chandrasekhar, Kaarthik S 2008 9 X X

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July 22, 2010 K493 K493 K493 K494 K494 K493 K493 K493 K493 K493 K494 698 698 K490 K493 K493 K493 K493 K493 K494 K494 K494 K490 K493 K494 K493 K493 K493 K493 K494 Desta, Muuz Zerusenay Doyon, Anthony Robert Sloan, Ashley Vaden Desta, Muuz Zerusenay Sloan, Ashley Vaden Dancy, Durell Lamond De La Rosa Jr, Manuel Carlos Leung, Anna Fown-Yee Mushonga, Pfumojena C Scott, Briana Janel Doyon, Anthony Robert Martin, Joseph Robert Chandrasekhar, Kaarthik S Dancy, Durell Lamond Dancy, Durell Lamond Flaig, Stephanie Marge Gonfa, Lemma G Leung, Anna Fown-Yee Mushonga, Pfumojena C De La Rosa Jr, Manuel Carlos Leung, Anna Fown-Yee Mushonga, Pfumojena C Eisenmenger, Brittany L Gonfa, Lemma G Gonfa, Lemma G Desai, Heli Ashish Kiesel, Anthony Douglas Desai, Heli Ashish Brown, Amber Nanette Desai, Heli Ashish 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2008 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2009 2010 2010 2010 1 1 1 1 1 1 1 1 1 1 1 6 6 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X

Mentoring Citations: Key: Level 1: Professors who had a remarkable and positive influence Level 2: Exceptional professors (Top 3) who had a remarkable and positive influence Level 3: Professors who especially influenced the whole course of a students life and had an extraordinary impact. Year 2007 2008 2009 Level 1 1 1 10 Level 2 1 1 5 Level 3 0 0 2

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July 22, 2010 Peer Teaching Evaluation by: Dr. David Stocum in 2007 fall Dr. Hiroki Yokota in 2009 spring Dr. Guoli Dai in 2009 fall New Course Development: 1. BME 352: CELL AND TISSUE BEHAVIOR AND PROPERTIES 2. BIOL 697 SPECIL TOPICS: MOLECULAR AND CELLULAR BONE BIOLOGY Mentor of Interns, Undergraduate Students and Non-thesis Graduate Students 2006 Fall Sarah E. Dolan (Biology Freshman Worker Program, IUPUI) Amelia Reiling, (Non-thesis M S student) 2007 Spring Sarah E. Dolan (Biology Freshman Worker Program, IUPUI) Rachel Meyer, (Biomedical Engineering, UROP) 2007 Fall Rachel Meyer, Top female student (UROP) Trevy A. Ramos, (Non-thesis M S student) Sharayah Marie Webb (Biology Freshman Worker Program, IUPUI) 2008 Spring Ian K. Ferries, (Non-thesis M S student) 2008 Summer Maycdon Sprowl (Project SEED Summer Research Program) Ian K. Ferries, (Non-thesis M S student) 2008 FallBrianna Scott (IUPUI Diversity Scholars Research Program) 2008 Fall Chelsea Quirk and Emily Royal (Biology Freshman Worker Program, IUPUI) 2009 Spring Chelsea Quirk (Biology Freshman Worker Program, IUPUI) Shuo Li and Tolga Caner (Non-thesis M S student) Mark Tyler Nelson (BME undergraduate) 2009 Summer Joshua P. Keith, Tyler Nelson, Brionna Davis IUPUI Multidisciplinary Undergraduate Research Institute (MURI) Erica Walsh, (Project SEED Summer Research Program) Michelle R. Archie (Bridges to the Baccalaureate) 2009 Fall Nicholas A. Jones (Biology Freshman Worker Program, IUPUI) Mark Tyler Nelson (BME undergraduate) 2010 Spring Nicholas A. Jones (Biology Freshman Worker Program, IUPUI) Mark Tyler Nelson (BME undergraduate) Teaching Philosophy I acquired my teaching interests and ideas from my experience being a student in China and Japan as well as being a researcher in Japan and United States. I graduated from one of the top medical schools in China and got extensive training in basic sciences and clinics. As an international student in Japan, I had studied and worked in the fields of Biomedical Engineering and Molecular Biology. Since then, my research theme has always been a combination of biology and engineering. Teaching courses related to both biology and engineering is my greatest satisfaction at IUPUI. When I started my teaching career at IUPUI in 2007, I had talked to experienced colleagues, learned from the experts at the Center for Teaching and Learning and attended many workshops and seminars to

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July 22, 2010 design new courses, improve my teaching skills and learn to use advanced teaching technologies. Peer evaluations further help me improve my teaching skills significantly. I always understand what I am teaching, prepare well-organized materials, and respect students. Course development has been a primary focus in rank. Since 2006, I have created two new courses, one undergraduate level course in the Department of Biomedical Engineering, School of Engineering IUPUI and one graduate level course in the Department of Biology, School of Science IUPUI. Because both of these two courses are related to my research interests in bone cell biology and regeneration, I really enjoy teaching these subjects. Students comment that the dynamic range of class sessions and my encouragement prompt them to do their best work. Almost all of my students hold my class in high regard, as the global scores given by the students range 3.52 to 4.55 out of 5. The BME352 Cell/Tissue Behavior and Properties course (3 credits) introduces the undergraduate students to the biological principles of cellular/tissue behaviors and properties. Topics include: fundamental concepts of cellular structure and tissue organization, biomolecular elements and their properties, cell shape, cell adhesion and migration, mechanotransduction, pattern formation in embryos, and stem cell and tissue regeneration. BIOL 697 Special Topics Molecular & Cell Bone Biology is a 3 credits graduate course. This course concentrates on the cellular and molecular concepts of bone and cartilage, and applications to engineering concepts. The topics include bone development and growth, cartilage and chondrocyte, signal transduction (intra- and intercellular signaling) in bone cells, mesenchymal stem cells and skeletal regeneration, tissue engineering (bone grafting), gene therapy, skeletal imaging, and cancer bone metastasis. This course is intend to provide fundamental molecular and cellular mechanisms relating to skeletal biology and disorders to graduate students in Biology, Anthropology and Biomedical Engineering at Indiana and Purdue University and IUPUI, students in the Basic Sciences at the IU School of Medicine and Dentistry, and clinical fellows in Orthopedic Surgery, Rheumatology and Endocrinology. Mentoring individual students to do research in the laboratory is another part of my teaching responsibility. I have supervised 22 undergraduate students in Biology and 2 undergraduate students in Biomedical Engineering, 6 non-thesis MS graduate students, 4 thesis MS candidates (3 in Biology and 1 in Biomedical Engineering) and 1 PhD candidates. Two MS graduate students graduated in 2009. One MS graduate is studying in IU School of Medicine. The other is a technician in a Medical Research Laboratory at IU. Working with students is also a great learning experience for me. I have learned how to guide students to look for a research topic, make a research plan, design experiments, analyze, interpret and present the data. I am convinced that training students in the lab is really important. I put a lot of effort into attracting students to my lab and help them gain research experience. Anthony Doyon received Undergraduate Research Award in 2009 and studies Podiatric Medicine. Heli Desai is the most Outstanding Bachelor of Science in Biology at 2010 and will enter a medical school. I chaired two committees of two Master Degree students. Currently I chair two committees for one Master Degree student and one Ph.D. Degree candidate. I am also a member of committee for one Master Degree student in Biomedical Engineering. I have taught the students the experimental techniques and supervised readings, study design,

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July 22, 2010 data analysis and thesis preparation. I have also supervised the Diversity Scholars Research Program and Independent Research and Capstone Research on a variety of topics with a senior Biology major. My future goals in teaching include: 1) to develop a set of materials and mechanisms to help students who are performing poorly, such as increase in office hours, addition of more animations and movies, lab tour, etc., 2) Textbooks and software for the courses I teach are not available. I have to list several books for students reference. I would like to write a textbook containing all the materials for each course in the future, 3) to attract more motivated students to participate in the research programs in my laboratory and help them succeed. RESEARCH My research interests are in skeletal tissue biology and regeneration. For many years I have studied the mechanisms by which exercise builds bone with the goal of identifying novel drug targets linked to increased bone strength. Recently, we have found a transcription factor called Stat3 plays an important role in mechanotransduction, a process that converts mechanical signals into biological signals in bone cells. Currently, our research has focused on the role of Stat3 in bone metabolism and fracture repair because patients with Stat3 mutations have osteoporosis and pathological fractures. We are also studying the application of vessel wall derived endothelial colony forming cells (ECFCs) in fracture repair and bone regeneration. We seed ECFCs in Type I collagen and hydroxyapatite and Tri-calcium phosphates (HA/TCP) scaffolds and use the cell-scaffold complexes to bone defect site to regenerate new bone tissues. Our long-term objectives are to identify drug targets for treatment of osteoporosis and bone damages. Significance of Research Ten million people in the US suffer from osteoporosis, a disease of reduced bone mass and debilitating fractures. Another 30-40 million in this country are at risk for this disease. Parathyroid hormone (PTH) is currently the only FDA-approved drug for osteoporosis that induces new bone growth, however, the molecular mechanisms responsible for this anabolic effect are not understood. Since Stat3 mediates mechanically induced bone formation, it is useful to investigate the potential roles of Stat3 as mediators of the skeletons anabolic response to PTH. Study of the roles of Stat3 in bone formation will provide new insight into molecular mechanisms underlying bone anabolic response. The novel Stat3 signaling in osteoblasts (bone-forming cells) could be a molecular switch that signals cells to increase bone formation. The findings will increase our knowledge of how osteoblastogenesis is regulated in vivo. We believe that the Stat3 signaling pathway has considerable potential for the identification of new treatment targets aimed at improving bone health. In particular, this project may provide new strategies to treat osteoporosis and prevent minimal trauma fracture in patients with Jobs Syndrome, a disease caused by Stat3 mutations. Furthermore, since Stat3 is also involved in cancer and cancer metastasis, understanding the roles of Stat3 in bone metabolism may help prevent and treat bone cancer metastasis. The repair of large bone and/or joint defects remains a major clinical orthopedic challenge. Bone is a highly vascularized tissue dependent on the close spatial and temporal connections between blood vessels and bone cells to maintain skeletal integrity.

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July 22, 2010 Angiogenesis plays a pivotal role in skeletal development and bone fracture repair. Current approaches to repair bone defects include the use of bone graft (autologus and allogeneic) or implants (polymeric and ceramic). These methods face significant limitations due to insufficient supply, potential diseases transmission, rejection, cost and the inability to integrate with the surrounding host tissue. The engineering of bone tissue offers new therapeutic strategies to improve skeletal repair. However, an active blood vessel network is an essential pre-requisite for these to survive and integrate with existing host tissue. Our goal is to produce bone grafts by combining stem cells and growth factor release scaffolds that can promote angiogenesis and osteogenesis. Grant Funding My goal is to continue to obtain external funding through National Institutes of Health (NIH), Department of Defense (DoD), National Science Foundation (NSF), and/or private research foundations. With the support of my previous award from National Aeronautics and Space Administration (NASA), we imported three mouse colonies in 2007 and generated bone specific Stat3 knockout mice. We have fully characterized the bone and loading phenotype of these mice. Our data suggest that Stat3 plays an important role in maintenance of bone mass and strength and mediates bone anabolic response to mechanical stimulation. Based on these preliminary data, I submitted a NIH RO1 grant application in October 2008. Although this application was not recommended for funding at that time, I am planning to revise this grant application and submit a new RO1 in 2010. In addition, our recent data disclose a close relationship between Stat3 and mitochondrial function. Knockout of Stat3 leads to inhibition of mitochondrial activity as demonstrated by decreased NADH oxidase activity and increased intracellular Reactive oxygen species (ROS) in osteoblasts. Based on these data, a NIH RO3 application was submitted in October 2009. The Impact/Priority score for this RO3 was 38. (The NIAMS RO3 payline is 28 this year.) I am planning to re-submit this RO3 in June 2010. For our bone regeneration project, we have successfully grown and characterized vessel derived ECFCs in culture. Animal studies using bone defect model in rats have shown ECFCs lead to new blood vessel formation and further accelerate fracture healing and induce new bone formation in vivo. In addition, we have built an Electrospinning System to produce nanofibers with a variety of biomaterials, including collagen, polymers, etc. Based on those data, I submitted 7 external grant applications and 2 internal grant applications in 2009 and the beginning of 2010 to seek funding to continue this project. One Department of Defense application and a NIH R21 application are pending. In addition, I get an opportunity to re-submit the internal grant application to Indiana Clinical and Translational Sciences Institute in 2010. Besides the abovementioned grant applications as Principal Investigator, I have been involved in 2 grant applications as Co-Investigator in 2010. As a Co-I, we received grant form Department of Defense through Special Congressional Appropriation (PI: Dr. David Stocum) in 2009. I am also a Co-I in a NIH UO1 (PI: Theresa Guise). I obtained one new internal award, IUPUI Multidisciplinary Undergraduate Research Institute (MURI) Mentorship Project in 2010. Overall, I have been very active to seek external funding. I believe that I am on the right track to produce high quality preliminary data to ensure our success in obtaining

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July 22, 2010 additional external funding in the near future. Patents & Disclosures A disclosure titled by Bone Regeneration using Endothelial Colony Forming Cells (#100081), was submitted to the Office of Technology Commercialization at January 12, 2010 by Jiliang Li and Mervin C. Yoder. Publications I have published 14 articles in peer-reviewed journals, including The Journal of Biological Chemistry, The Journal of Bone and Mineral Research, Bone, Osteoporosis International, Calcified Tissue International, Diabetes, Genomics, The Journal of Orthopedic Research and The Journal of Musculoskeletal and Neuronal Interaction, since 2006. One of the figures that I produced for one manuscript is featured on the cover of December 2009 issue of The Journal of Bone and Mineral Research, the most prestigious journal in bone field. In addition, three manuscripts were revised and re-submitted. Two more manuscripts are in preparation and will be submitted this year. Presentations I participated in local, national and international meetings regularly. I have presented my own work at least 2-3 meetings at the national conferences per year. Collaborations My collaborators include: 1. David Burr, Ph.D. Professor and Chair, Department of Anatomy and Cell Biology, IU School of Medicine. Healing of stress fracture in an animal model and Imaging microdamage in vivo 2. Charles Turner, Ph.D., Chancellor Professor and Director, Department of Biomedical Engineering, IUPUI and Orthopedic Research Laboratory, IU School of Medicine. Mechanisms of stochastic resonance on bone cells and Mechanotransduction: Puringergic Signaling in Bone 3. Mervin C. Yoder, M.D. Endothelial Colony Forming Cell for Bone Regeneration 4. Hiroki Yokota, Ph.D. Professor of Biomedical Engineering, IUPUI. Mitochondrial function in bone metabolism 5. Zhong-Yin Zhang, Ph.D. Professor and Chair, Department of Biochemistry and Molecular Biology, IU School of Medicine. Chemical Genomics approach to discover useful small molecular compounds for bone regeneration 6. Randall Duncan, Ph.D. Professor and Chair, Department of Biology, University of Delaware. Mechanotransduction: Puringergic Signaling in Bone 7. Xin-Yuan Fu, Ph.D. Professor, Department of Microbiology and Immunology, IU School of Medicine. Stat3 signaling in Bone 8. Stuart Warden, Ph.D., IU School of Health & Rehabilitation Sciences. Healing of stress fracture in an animal model 9. Siu L. Hui, Ph.D. Professor, Division of Biostatistics, Department of Medicine, IU School of Medicine Healing of stress fracture in an animal model 10. Jake Chen, Ph.D. School of Bioinformatics. An integrated proteomics analysis of bone tissues in response to mechanical stimulation

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July 22, 2010 11. Randall Roper, Ph.D. Department of Biology, School of Science. Bone structure and mechanical properties of Down Syndrome mice 12. David Stocum, Ph.D. Department of Biology, School of Science. Regenerative Medicine for Battlefield Injuries and Development of the Axolotl as a Research Model for Musculoskeletal Segment Defect Regeneration 13. Dong Xie, Ph.D., Department of Biomedical Engineering, IUPUI. Innovative All-in-one High-performance Dental Restorative System 14. Fengchun Yang, Ph.D., Department of Pediatrics, IU School of Medicine. The NF1 gene regulates skeletal remodeling and repair 15. Lilian Plotkin, Ph.D. Indiana University School of Medicine currently, University of Arkansas for Medical Sciences previously. Connexin 43 hemichannels and signaling in bone 16. Jun You, PhD. The Pennsylvania State University College of Medicine. Molecular Basis of Mechanotransduction in Bone Cells 17. Agnes Vignery, Ph.D. Associate Professor, Department of Orthopaedics and Cell biology, Yale School of Medicine. 18. OP van der jagt, Ph.D. Orthopaedic Research Laboratory, Erasmus MC, The Netherlands. Unfocused extracorporeal shock waves induce anabolic effects in rat bone 19. Therasa Guise, Ph.D. Professor, University of Virginia. Stat3 mediates TGF-beta Receptor R1 signaling in cancer bone metastasis Future plans My research goals for the next several years include: 1) to obtain additional external funding from the national Institutes of Health, Department of Defense, and/or National Science Foundation, 2) to publish more papers in high impact scientific journals, and 3) to obtain the national and international recognition for my research program. SERVICE My service has been at the departmental, School of Science and IUPUI levels. 1) I have worked with Dr. Teri Belecky-Adams to organize our department seminars since January 2008. I chaired two graduate committees. I am in Graduate Cellular and Developmental Biology Qualifying Exam Committee. I served in Search Committee for Faculty in Forensic Biology 2009. I also served in the Search Committee for Faculty in Biology 2009 - 2010. 2) I have been in School of Science Vivarium committee to plan a new research building. 3) At campus level, I have been a reviewer for IUPUI Undergraduate Research Opportunities Program (UROP), Committee for Bowling-JonesRusso Award and Chancellors Award for Undergraduate Research, and mentor for the Diversity Scholars Research Program and Project SEED Summer Research Program, Indiana Section, American Chemical Society. I am also a mentor for Multidisciplinary Undergraduate Research Institute (MURI) team. I have also been involved in community service. I was a judge in Hoosier Science and Engineering Fair at 2007, and facilitated Cell Biology competition in Indiana State Science Olympiad twice. Since 2006, I have reviewed 14 manuscripts for 5 peer-reviewed journals. I had also review 2 grant proposals for two state research organizations.

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July 22, 2010 My future goals in service are: to Chair a committee, to continue serving the school of Science and IUPUI, and to serve in a professional society or association at the national level.

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Anna L. Malkova, PhD

CURRICULUM VITAE Name: Anna Malkova, Ph.D. Present Rank: Associate Professor of Biology Personal Data: Born: St. Petersburg, Russia, July 15, 1965 Business Address: Department of Biology IUPUI, SL336 723 West Michigan St. Indianapolis, IN 46202 Phone: 317-278-5717 Fax: 317-274-2846 e-mail: amalkova@iupui.edu EDUCATION AND TRAINING Ph.D./1993 B.S./1987 St. Petersburg State University, St. Petersburg, Russia, Genetics. St. Petersburg State University, St. Petersburg, Russia, Genetics.

Postdoctoral Training: 2000-2003 Research Associate, Brandeis University, Waltham, MA. 1997-2000 Postdoctoral Fellow, Brandeis University, Waltham, MA. (Mentor: J.E. Haber). 1993-1996 Postdoctoral Fellow, Brandeis University, Waltham, MA. (Mentor: J.E. Haber). ACADEMIC APPOINTMENTS 2009- present 2003-2009 2000-2003 1997-2000 1996-1997 1993-1996 1987-1993 Associate Professor, Department of Biology, Indiana UniversityPurdue University Indianapolis. Assistant Professor, Department of Biology, Indiana University-Purdue University Indianapolis. Research Associate, Brandeis University, Waltham, MA. Postdoctoral Fellow, Brandeis University, Waltham, MA. Staff Scientist, St. Petersburg State University, St. Petersburg, Russia. Postdoctoral Fellow, Brandeis University, Waltham, MA. Graduate student, St. Petersburg State University, St. Petersburg, Russia.

OTHER APPOINTMENTS 10/2005-present Adjunct Assistant Professor, Department of Medical and Molecular Genetics, Indiana University School of Medicine.

Anna L. Malkova, PhD

PROFESSIONAL ORGANIZATIONS 2006-present 2000-present American Society for Microbiology Genetic Society of America

HONORS AND AWARDS 2009 2010 Outstanding Leadership and Mentoring of Undergraduate Research IUPUI Center for Research and Learning Award. School of Science Research Award

PROFESSIONAL SERVICE Manuscript Reviews (08/2003- present) Ad hoc reviewer for the following magazines: Mutation Research, Genome Research, Molecular Cell, Molecular and Cellular Biology, Genetics, PLoS Genetics, Genes and Development, Nucleic Acid Research. Grant Application Panel Member -Served as a reviewer for the following NIH study sections:
ZGM1 MBRS (2008), and GGG (2006, 2007).

-Served as an ad hoc reviewer for the Wellcome Trust panel, Molecules, Genes, and Cells Department, in 2007 and 2009. INVITED SEMINARS. 1. 2. 3. 4. 5. 5. 6. 7. Eppley Seminar Series, University of Nebraska Medical School, Omaha, October 21, 2009. Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, May 30, 2008. Laboratory of Molecular Genetics, National Institutes of Environmental Health Science, Research Triangle Park, NC, May 17, 2007. Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, TX, Aug 1, 2006. Department of Medical and Molecular Genetics, School of Medicine, IUPUI, Indianapolis, IN, Sept 30, 2005. (Presentation in the Medical and Molecular seminars series.) Department of Medical and Molecular Genetics, School of Medicine, IUPUI, Indianapolis, IN, May 18, 2005. (Presentation in the Genetics Research Club series.) Department of Biochemistry, School of Medicine, IUPUI, Indianapolis, IN, May 4, 2004. Department of Agronomy, Purdue University, West Lafayette, IN, Feb 18, 2004. 2

Anna L. Malkova, PhD

RESEARCH GRANTS. EXTERNAL NIH, 1R01GM084242-01 Amplification of risk resulting from mis-routing of double-strand break repair. Dates: 7.01.08 - 6.30.13 Funding Level: $1,341,000 Role on the grant: PI NIH, 1R03ES016434-01 Visualization of break-induced replication. Funding Level: $179,862 Role on the grant: PI NIH, 1R15GM074657-01A1 Mechanism of break-induced replication in yeast. Funding Level: $207,000 Role on the grant: PI NIH, Supplemental, 3R15GM074657-01A1S1 Mechanism of break-induced replication in yeast. Funding Level: $34,000 Role on the grant: PI INTERNAL MURI Research Grant Development and Implementation of Novel Biosensors for Mutagenic Exposures Dates: 11.01.09 - 5.15.10 Funding Level: $14,000 Role on the grant: PI PRF Research Grant Investigating the genetic consequences of failed break-induced replication. Dates: 6.01.08 - 5.31.09 Funding Level: $16,375 Role on the grant: PI RSFG Analysis of molecular intermediates of break-induced replication. Funding Level: $35,000 Role on the grant: PI RSFG Mechanism of break-induced replication in yeast. Funding Level: $30,000 Role on the grant: PI Dates: 7.01.07 - 6.30.08 Dates: 7.01.08 - 6.30.10

Dates: 3.01.06 - 2.28.09

Dates: 8.21.06 - 2.28.09

Dates: 1.01.06 - 12.31.06

Anna L. Malkova, PhD American Cancer Society (Institutional grant) Mechanism of BIR and gross chromosomal rearrangements. Funding Level: $20,000 Role on the grant: PI Grant-in-Aid for Research Identification of molecular mechanisms of break-induced replication. Dates: 4.15.04 - 4.14.05 Funding Level: $3,000 Role on the grant: PI Dates: 5.15.04 -7.15.04 PRF Summer Research faculty grant Mechanisms of DSB DNA repair by break-induced replication. Funding Level: $7,000 Role on the grant: PI Grant-In-Aid for women (OPD) Mechanisms of gross chromosome rearrangements. Funding Level: $1,500 Role on the grant: PI

Dates: 1.01.06 - 12.31.06

Dates: 1.01.04 -12.31.04

RESEARCH PUBLICATIONS Angela Deem, Andrea Keszthelyi, Tiffany Blackgrove, Alexandra Vayl, Barbara Coffey, Ruchi Mathur, Andrei Chabes, and Anna Malkova. .Break-induced Replication Is Highly Inaccurate, PLoS Biol., in press. Chung Y., Zhu, Papusha A., MalkovaA., and Ira.G. Elimination of processive resection at DNA double-strand breaks leads to de novo telomere formation and enhances gene targeting, pending minor revisions in PLoS Genet. (2010), 6 (5): e1000948. Westmoreland J, Ma W, Yan Y, Van Hulle K, Malkova A, Resnick MA. RAD50 is required for efficient initiation of resection and recombinational repair at random, gammainduced double-strand break ends. PLoS Genet. (2009), ;5(9):e1000656 Downing B., Morgan R., VanHulle K., Deem A., and Malkova A. Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51 Mutation Research, (2008), 645:9-18. Deem A., Barker K., VanHulle K., Downing B., Vayl A., and Malkova A. Defective break-induced replication leads to half-crossovers in Saccharomyces cerevisiae. Genetics, (2008), 179: 1845-1860. VanHulle K., Lemoine F. J., Narayanan V., Downing B., Hull K., McCullough C., Bellinger M., Lobachev K., Petes T. D., and Malkova A. Inverted DNA repeats channel repair of distant double-strand breaks into chromatid fusions and chromosomal rearrangements. Mol. Cell. Biol., (2007), 27: 2601-2614.

Anna L. Malkova, PhD Malkova A., Naylor A.L., Yamaguchi M., Ira G., and Haber J. RAD51-dependent breakinduced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion. Mol. Cell. Biol., (2005), 25: 933-944. Malkova A., Swanson J., German M., McCusker J.H., Housworth E.A., Stahl F.W., and Haber J.E. Gene conversion and crossing-over along the 405-kb left arm of Saccharomyces cerevisiae chromosome VII. Genetics, (2004), 168: 49-63. Ira G., Malkova A., Liberi G., Foiani M., and Haber J.E. Srs2 and Sgs1-Top3 suppress crossovers during double-strand break repair in yeast. Cell, (2003), 115: 401-11. Lee S.-E., Pellicioli A., Sugawara N., Vaze M., Malkova A., Foiani M., and Haber J. Yeast Rad52 and Rad51 recombination proteins define a second pathway of DNA damage assessment in response to a single double-strand break. Mol. Cell. Biol., (2003), 23: 8913-8923. Malkova A., Signon L., Schaefer C., Naylor M., Murakami H., Ohta K., Newlon C., and Haber J. RAD51-independent break-induced replication to repair a broken chromosome depends on a distant enhancer site. Genes Dev., (2001), 15: 1055-1060. Signon L., Malkova A., Naylor M., Klein H., and Haber J. Genetic requirements for breakinduced replication repair of a chromosomal double-strand break. Mol. Cell. Biol., (2001), 21: 2048-2056. Lee S.-E., Pellicioli A., Malkova A., Foiani M., and. Haber J.E. The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a single double-strand break. Curr. Biol., (2001), 11: 1053-1057. Malkova A., Klein F., Leung W.-Y., and Haber J.E. HO endonuclease-induced recombination in yeast meiosis resembles Spo11-induced events. Proc. Natl. Acad. Sci. USA, (2000), 97: 14500-14505. Lee S.E., Pellicioli A., Demeter J., Vaze M.P., Gasch A.P., Malkova A., Brown P.O., Botstein D., Stearns T., Foiani M., and Haber J.E. Arrest, Adaptation, and Recovery following a chromosome double-strand break in Saccharomyces cerevisiae. Cold Spring Harbor Symposia on Quantitative Biology, Vol. LXV, Cold Spring Harbor Laboratory Press, (2000), 303-313. Leung W., Malkova A., and Haber J.E. Gene targeting by linear duplex DNA frequently occurs by assimilation of a single strand that is subject to preferential mismatch correction. Proc. Natl. Acad. Sci. USA, (1997), 94: 6851-6856. Malkova A., Ivanov E.L., and Haber J.E. Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication. Proc. Natl. Acad. Sci. USA, (1996), 93: 7131-7136.

Anna L. Malkova, PhD Malkova A., Ross D., Dawson M., Hoekstra F., and Haber, J. E. Meiotic recombination initiated by a double-strand break in rad50 delta yeast cells otherwise unable to initiate meiotic recombination. Genetics, (1996), 143: 741-754. Gordenin D.A., Lobachev K.S., Degtyareva N.P., Malkova A.L, Perkins E, and Resnick M.A. Inverted DNA repeats: a source of genomic instability. Mol. Cell. Biol., (1993), 13: 5315- 5322. Gordenin D.A., Malkova A.L., Petersen A., Kulikov V.N., Pavlov Y.I., Perkins E., and Resnick M.A. Transposon Tn5 excision in yeast: the influence of DNA polymerases alpha, delta, epsilon and repair genes. Proc. Natl. Acad. Sci. USA, (1992), 89: 3785-3789. Gordenin D.A., Prosciavicus Y.Y., Malkova A.L., Trofimova M.I., and Petersen A. Yeast mutants with increased bacterial transposon Tn5 excision. Yeast, (1991), 7: 37-59. BOOK CHAPTERS AND REVIEW ARTICLES . ABSTRACTS Malkova A., Downing B., VanHulle K., and Deem A. Single-strand annealing between inverted repeats initiated by double-strand breaks. Abstracts of the FASEB Summer Research Conference DNA palindromes: roles, consequences and implications of structurally ambivalent DNA, 2008: 6. Deem A., VanHulle K., Downing B., Hull K., Morgan R., and Malkova A. Deleterious pathways channeling repair of double-strand breaks into chromosomal rearrangements. Abstracts of the FASEB Summer Research Conference Genetic recombination and genome rearrangements, 2007: 70. VanHulle K., Lemoine F. J., Narayanan V., Downing B., Hull K., McCullough C., Bellinger M., Lobachev K., Petes T. D., and Malkova A. Inverted DNA repeats channel repair of distant double-strand breaks into chromatid fusions and chromosomal rearrangements. Abstracts of the FASEB Summer Research Conference Yeast chromosome structure, replication, and segregation, 2006: B23. VanHulle K., Lemoine F. J., Narayanan V., Downing B., Hull K., McCullough C., Bellinger M., Lobachev K., Petes T. D., and Malkova A. Inverted DNA repeats channel repair of distant double-strand breaks into chromatid fusions and chromosomal rearrangements. Abstracts of the Gordon Research Conference Mutagenesis, 2006: T12. Deem A., VanHulle K., Lemoine F. J., Narayanan V., Downing B., Hull K., McCullough C., Bellinger M., Lobachev K., Petes T. D., and Malkova A. Inverted DNA repeats channel repair of distant double-strand breaks into chromatid fusions and chromosomal rearrangements. Abstracts of the SALK Institute Research Conference on DNA replication and genomic integrity, 2006: 76. Haber J.E., Ira G., Malkova A., and Sugawara N. Repairing a double-strand chromosome break by homologous recombination: revisiting Robin Holliday's model. Philos. Trans. R. Soc. Lond. B. Biol. Sci., (2004), 359(1441): 79-86.

Anna L. Malkova, PhD VanHulle K., Lemoine F. J., Narayanan V., Downing B., Hull K., McCullough C., Bellinger M., Lobachev K., Petes T.D., and Malkova A. Inverted DNA repeats channel repair of distant double-strand breaks into chromatid fusions and chromosomal rearrangements. Abstracts of the 8th Annual Midwest DNA repair symposium, 2006: 12. Bellinger M., Lemoine F., VanHulle K., Hull K., Noble E., Petes T., and Malkova A. The mechanism of chromosomal rearrangements resulting from RAD51-independent repair of DNA breaks. Abstracts of the FASEB Summer Research Conference Genetic recombination and genome rearrangements, 2005: 70. Malkova A. Characterization of gross chromosomal rearrangements resulting from RAD51independent repair of DNA breaks. Abstracts of the FASEB Summer Research Conference Yeast chromosome structure, replication, and segregation, 2004: B7. Malkova A., Naylor M.-A., Yamaguchi M., Haber J.E. RAD51-dependent break-induced replication differs in kinetics and checkpoint responses from RAD51-mediated gene conversion. Abstracts of the FASEB Summer Research Conference Genetic recombination and genome rearrangements, 2003: 63. Malkova A., Ira G., Yamaguchi M., Naylor M., and Haber J.E. Mechanisms of breakinduced replication and the maintenance of telomeres without telomerase. Abstracts of the papers presented at the 2003 meeting on Telomeres and Telomerase, 2003: 197. Haber J.E., Lee S-E., Vaze M.B., Malkova A., Pellicioli A., and Foiani M. Checkpoint responses and repair of a single broken chromosome in Saccharomyces. Abstracts of the papers presented at the at the Weimar meeting on DNA repair, 2000. Haber J., Lee S-E., Vaze M., Malkova A., Naylor M., Signon L., Holmes A., and Sugawara N. Checkpoint responses and repair of double-strand breaks in Saccharomyces. Abstracts of the meeting of the American Association of Cancer Research, 2000: 47. Malkova A., Ira G., Yamaguchi M., Naylor M., Kraus E., and Haber J.E. Role of breakinduced replication in repair of double-strand breaks in yeast. Abstracts of the National Academy of Sciences Colloquium Links between Recombination and Replication: Vital Roles of Recombination, 2000. Haber J.E, Ira G., Malkova A., Sugawara N. and Naylor M. Exploring chromosome structure and function by recombination. Abstracts of the FASEB Summer Research Conference Yeast Chromosome Structure, Replication and Segregation, 2000: T22. Malkova A., Klein F., Leung W.-Y., and Haber J.E. Meiotic and Mitotic pathways of HOinduced double-strand break repair in the yeast Saccharomyces cerevisiae. Abstracts of the Gordon research Conference Meiosis, 2000: P23. Lee S.E., A. Pellicioli, Demeter J., Vaze M.P., Gasch A.P., Malkova A., Brown P.O., Botstein P.O., Stearns T., Foiani M., and Haber J.E. Genetic Analysis of Adaptation process from the damage-inducible G2/M cell cycle checkpoint in Saccharomyces cerevisiae. LXV Cold Spring Harbor Symposium on Quantitative Biology Biological Responses to DNA damage, 2000. Malkova A., and Haber J.E. Double-strand break repair and crossing-over in meiosis and mitosis. Abstracts of the 4th European Meiosis Meeting The Meiotic process: Nuclear Architecture, Recombination and Chromosomal Segregation, 1999: 6. Haber J.E., Bosco G., Colaiacovo M., Holmes A., Lee S-E., Malkova A., Paques F., Richard G.-F., and Sugawara, N. Multiple pathways of double-strand break repair in Saccharomyces: three model systems for human diseases. Abstracts of the conference on Yeast Genetics and human disease, 1999.

Anna L. Malkova, PhD Haber J.E., Bosco G., Colaiacovo M., Holmes A., Lee S.-E., Malkova A., Paques F., Richard G.-F., and Sugawara, N. Recombination and replication in Saccharomyces. Abstracts of the Keystone Symposia on Molecular and Cellular Biology Molecular Mechanisms in DNA Replication and Recombination, 1999: T21. Haber J.E., Bosco G., Colaiacovo M., Holmes A., Malkova A., Paques F., Richard, G.-F., and Sugawara, N. Recombination and replication in Saccharomyces. Abstracts of the DNA repair meeting in Japan, 1998: 28. Malkova A., Leung W.-Y., and Haber JE. Comparing of meiotic and mitotic repair of HOinduced DNA breaks in yeast. Abstracts of the Gordon Research Conference Meiosis, 1998: P20. Malkova A., Ross L., Dawson M., Hoekstra F. and J. E. Haber. Meiotic repair of HOinduced DNA breaks in the yeast Sacharomyces cerevisiae. Abstracts of the Gordon Research Conference Meiosis, 1996: P25. Haber J.E., Ivanov E., Sugawara N., Malkova A., and Bosco G. Mechanisms of Homologous and Nonhomologous Recombination in Saccharomyces cerevisiae. Abstracts of the Keystone Symposia on Molecular and Cellular Biology Molecular Mechanisms in DNA Replication and Recombination, 1996: T22. Malkova A. and Haber J.E. Competition between different repair events during mitosis and meiosis in yeast. Abstracts of the FASEB Summer Research Conference Yeast Chromosome Structure, Replication and Segregation, 1994: B28. Malkova A. and Haber J.E. Competition between different repair events during mitosis and meiosis in yeast. Abstracts of the Gordon Research Conference Meiosis, 1994: P12. Gordenin D.A., Malkova A.L., Petersen A., Kulikov V.N, Pavlov Y.I., Perkins E., and Resnick M.A. Marker-effect of bacterial transposon Tn5 in intragenic recombination in yeast. Abstracts of the Sixth Congress of N.I. Vavilov All-Union Society of Genetics and Breeding, 1992: 31.

Curriculum Vitae James Alan Marrs Personal: Born November 4, 1962 Married to Dr. Kathleen A. Marrs, 3 Children Education: University of Illinois at Urbana-Champaign, B. S. in Biology 1984. University of Illinois at Chicago, Ph. D. in Biology 1991. Advisor: Dr. G. Benjamin Bouck. Stanford University School of Medicine, Postdoctoral training in epithelial cell biology, 1991-1994. Sponsor: Dr. W. James Nelson. Academic Appointment: Assistant Professor of Medicine and Cellular & Integrative Physiology, Indiana University Medical Center. January 1995 to June 2001. Associate Professor of Medicine and Cellular & Integrative Physiology, Indiana University Medical Center. July 2001-August 2008. Associate Professor of Biology, Purdue University School of Science, and Associate Professor of Medicine and Cellular & Integrative Physiology, Indiana University School of Medicine. August 2008-present. Other Employment: Founding Member, INphoton, LLC, 2005-present. Manager 2005-2009. INphoton performs specialized and customized confocal and 2-photon microscopy studies for pharmaceutical and biotechnology companies. For most clients, we perform intravital microscopy, which is 2photon microscopy to visualize and measure physiological parameters in organs and tissues (kidney, liver, brain, tumors, etc.) of living rodents (see www.inphoton.com). Professional Organizations: Member, American Society for Cell Biology. Honors and Awards: National Eye Institute fellowship to attend the Fundamental Issues in Vision Research course at Woods Hole Marine Biology Laboratory, August 1994. Laboratory for Molecular Biology, University of Illinois at Chicago, predoctoral fellowship, 19891990. Invited Seminars: Indiana State University, Department of Biology, Terre Haute IN, November 2010. Indiana Clinical and Translational Sciences Institute (CTSI) Retreat, panel discussion, Purdue University, West Lafayette, September 2009. Indiana University-Purdue University Indianapolis, Department of Biology, May 2008. University of Tennessee, Department of Biology, May 2008. 1

Symposium on Tight Junctions, Annual Meeting of the American Society of Nephrologists, November 2007. Indiana University Bloomington, Biocomplexity Institute, Department of Physics, November 2005. Wabash College, Department of Biology, October 2005. University of Illinois at Chicago, Department of Biological Sciences, April 2005. Eli Lilly and Company, Investigational Toxicology, November 2004. Indiana University School of Medicine - Northwest Center, Department of Medical Sciences, October 2004. American Society for Cell Biology 2002 Annual Meeting, San Francisco CA, December 2002. University of Cincinnati, Departments of Surgery and Cell Biology, April 2002. Eighth International Workshop on Developmental Nephrology, International Pediatric Nephrology Association, Victoria, BC, August 2001. Medical College of Wisconsin, Department of Cell Biology, Neurobiology and Anatomy, October 2000. Stanford University, Department of Molecular and Cellular Physiology, April 2000. Indiana University School of Medicine - Evansville Center, Department of Medical Sciences, March 1999. University of North Carolina, Department of Physiology, March 1999. Cornell University Medical Center, Department of Biochemistry, June 1998. Organizer and Moderator, Symposium on Cell-Cell Adhesion Junctions, Annual Meeting of the American Society of Nephrologists, November 1997. Indiana University School of Medicine, Department of Medicine, Division of Endocrinology, October 1997. Indiana University School of Medicine - Northwest Center, Department of Medical Sciences, April 1997. Indiana University School of Medicine, Department of Biochemistry and Molecular Biology, February 1997. Purdue University, Department of Basic Medical Sciences, December 1996. University of Michigan, Department of Anatomy and Cell Biology, May 1996. University of Michigan, Department of Internal Medicine, May 1996. University of Illinois, Department of Biological Sciences, April 1996. Indiana University School of Medicine, Department of Anatomy, November 1995. Indiana University School of Medicine, Department of Physiology and Biophysics, October 1994. FASEB 1994 Annual Meeting, Anaheim CA, April 1994. The University of Georgia, Department of Zoology, February 1994. Indiana University School of Medicine, Department of Medicine, February 1994. Stanford University, Department of Medicine, January 1994. Washington University, Department of Cell Biology, November 1992. Fox Chase Cancer Center, October 1989. Annual Meeting, American Society of Plant Physiologists, Reno NV, July 1988. Teaching Assignments: Full Course (16 weeks, two 1.25 hour sessions per week). Indiana University-Purdue University Indianapolis. Cell Biology (BIOL K324). Fall Semester 2010. Full Course (16 weeks, 20 hours per week per student, 5 students). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 493). Fall Semester 2010.

Full Course (16 weeks, 2 student theses). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 494). Fall Semester 2010. Full Course (16 weeks, two 1.25 hour sessions per week). Indiana University-Purdue University Indianapolis. Stem Cell Biology (BIOL 697). Spring Semester 2010. Full Course (16 weeks, 1 student project). Indiana University-Purdue University Indianapolis. Capstone in Biology (BIOL 490). Spring Semester 2010. Full Course (16 weeks, 20 hours per week per student, 2 students). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 493). Spring Semester 2010. Full Course (16 weeks, 1 student thesis). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 494). Spring Semester 2010. Full Course (16 weeks, two 1.25 hour sessions per week). Indiana University-Purdue University Indianapolis. Stem Cell Biology (BIOL 697). Fall Semester 2009. Full Course (16 weeks, 1 student project). Indiana University-Purdue University Indianapolis. Capstone in Biology (BIOL 490). Fall Semester 2009. Full Course (16 weeks, 20 hours per week per student, 4 students). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 493). Fall Semester 2009. Full Course (16 weeks, 2 student theses). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 494). Fall Semester 2009. Full Course (16 weeks, two 1.25 hour sessions per week). Indiana University-Purdue University Indianapolis. Stem Cell Biology (BIOL 697). Spring Semester 2009. Full Course (16 weeks, 20 hours per week per student, 5 students). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 493). Spring Semester 2009. Full Course (16 weeks, 3 student theses). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 494). Spring Semester 2009. Full Course (16 weeks, 20 hours per week per student, 4 students). Indiana University-Purdue University Indianapolis. Undergraduate Research (BIOL 493). Fall Semester 2008. Team Taught Course (0.5 week, two 1.5 hour session per week). Indiana University Medical Center. Cellular Basis of Systems Biology (G717). Fall Semester 2007. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Cellular Physiology (F710). Fall Semester 2006. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Cellular Physiology (F710). Fall Semester 2005. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Spring Semester 2004. Team Taught Course (0.5 weeks, two 1.5 hour sessions per week). Indiana University Purdue University-Indianapolis, Department of Biology. Topics in Biotechnology (B540). Fall Semester 2003. Team Taught Course (1 week, five 30 minute lectures, and two 1 hour laboratory demonstrations per week). Indiana University Medical Center. Advanced Molecular Biology Methods (G910). Summer II Semester 2003 Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Spring Semester 2003. Team Taught Course (0.5 weeks, two 1.5 hour sessions per week). Indiana University Purdue University-Indianapolis, Department of Biology. Topics in Biotechnology (B540). Fall Semester 2002. Team Taught Course (1.5 weeks, two 1.5 hour sessions per week). Indiana University Purdue University-Indianapolis, Department of Biology. Topics in Biotechnology (B540). Fall Semester 2001. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 2001. 3

Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 2000. Co-Director. Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). Spring Semester 2000. Team Taught Course (2 weeks, one 3 hour sessions per week). Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). Spring Semester 2000. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 1999. Team Taught Course (2 weeks, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 1998. Co-Director. Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). 1998. Team Taught Course (4 weeks, one 3 hour sessions per week). Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). Spring Semester 1998. Team Taught Course (2.5 weeks, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 1997. Team Taught Course (2.5 weeks, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 1996. Co-Director. Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). 1996. Team Taught Course (5 weeks, two 90 minute sessions per week). Indiana University Medical Center. Epithelial cell biology (G760 Cellular Basis of Renal Function). Spring Semester 1996. Team Taught Course (1 week, two 2 hour sessions per week). Indiana University Medical Center. Molecular and Cellular Physiology (F705). Fall Semester 1995. Indiana University Medical Center. Committee for establishing a graduate course on imaging technologies in cell biology research. 1995. Teaching Assistant. Stanford University School of Medicine. Cell Biology of Physiological Processes. 1994. Other Teaching: Mentor, Research Associate: Swapnalee Sarmah. 2010-present. Mentor, Graduate Student, Department of Biology. Student: Teddy Moskowinski. 2009-present. Mentor, Postdoctoral Fellow, Research Associate, Assistant Scientist: Shobha Gopalakrishnan, PhD. August 1996- 2006. Current position: Scientist, Alba Pharmaceuticals. Mentor, Postdoctoral Fellow, Research Associate: Sherry Babb, PhD. June 1999- 2006. Current position, Research Assistant Professor, Indiana University School of Medicine. Mentor, Graduate Student, Department of Physiology and Biophysics. Student: Matthew Rendel. 1999-2000. Mentor, Graduate Student, Department of Physiology and Biophysics. Student: Brad Poteat. 19981999. Mentor, Graduate Student, Department of Physiology and Biophysics. Student: Kristi Sanborn. 1997998. Co-Mentor (with Dr. James Nelson), MSTP Student, Department of Molecular and Cellular Physiology, Stanford University School of Medicine. Student: Megan Troxell. 1995-2000. Mentor, Undergraduate Summer Biomedical Research Program. Student: Catherine Chung. Summer 1995.

Professional Service: Grant and Journal Review: Editorial Board Member, American Journal of Physiology: Cell Physiology: 2002-present. Editorial Board Member, American Journal of Physiology: Renal Physiology: 2010-present. Ad hoc reviewer, American Journal of Physiology. Ad hoc reviewer, Cell Adhesion and Communication. Ad hoc reviewer, Cell and Molecular Biology. Ad hoc reviewer, Developmental Dynamics. Ad hoc reviewer, Genesis. Ad hoc reviewer, Investigative Ophthalmology and Visual Sciences. Ad hoc reviewer, Journal of Cell Biology. Ad hoc reviewer, Journal of Cell Science. Ad hoc reviewer, Journal of Cellular Biochemistry. Ad hoc reviewer, Journal of Histochemistry and Cytochemistry Ad hoc reviewer, Journal of the American Society of Nephrology. Ad hoc reviewer, Kidney International. Ad hoc reviewer, Mechanisms of Development. Ad hoc reviewer, Molecular Biology of the Cell. Ad hoc reviewer, Oncogene. Ad hoc reviewer, PLOS Biology. Ad hoc reviewer, PLOS One. Ad hoc reviewer, Zebrafish. Ad hoc reviewer, National Science Foundation. July 1997. Ad hoc reviewer, VISC study section, National Institutes of Health. February 1998. Ad hoc reviewer, VISC study section, National Institutes of Health. October 1998. Ad hoc reviewer, Veterans Administration. September 1998. Ad hoc reviewer, NICHD Program project grant, National Institutes of Health. April 1999. Ad hoc reviewer, VISC study section, National Institutes of Health. October 1999. Ad hoc reviewer, VISC study section, National Institutes of Health. February 2000. Ad hoc reviewer, NICHD Program project grant, National Institutes of Health. April 2000. Ad hoc reviewer, National Institutes of Health, Special Emphasis Panel. July 2000. Ad hoc reviewer, Veterans Administration. September 2001. Ad hoc reviewer, NIDDK Program project grant, National Institutes of Health. December 2003 Ad hoc reviewer, NDPR study section, National Institutes of Health. February 2004. Ad hoc reviewer, Medical Research Council UK. August 2004. Ad hoc reviewer, CMBK study section, National Institutes of Health. February 2005. Ad hoc reviewer, Special Emphasis Panel related to Renal Transport and PKD, National Institutes of Health. July 2005. Ad hoc reviewer, LIRR Study Section, National Institutes of Health. October 2005. Ad hoc reviewer, LIRR Study Section, National Institutes of Health. June 2006. Ad hoc reviewer, MDCN-K Study Section, National Institutes of Health. June 2006. Ad hoc reviewer, MDCN-K Study Section, National Institutes of Health. March 2007. Ad hoc reviewer, NINDS Fellowship Study Section, National Institutes of Health. June 2007. Ad hoc reviewer, NINDS Fellowship Study Section, National Institutes of Health. March 2008. Ad hoc reviewer, Special Emphasis ZRG1 ETTN-A Study Section, National Institutes of Health. June 2009. 5

Ad hoc reviewer, CB-J (02) Study Section, National Institutes of Health. December 2009. University Service: Departmental: Member, Graduate Committee. Department of Cellular and Integrative Physiology. 2002-2005. Member, Search Committeee (Academic Specialist). Department of Biology. 2009. Member, Search Committeee (Faculty). Department of Biology. 2009. Member, Graduate Committee. Department of Biology. 2010-present. Member (Biology representative), School of Science Research Committee. 2008-present. Elected Member, IUPUI Faculty Council. 1998-2000. Member, Biomedical Research Committee. 2005-2008 Member, RSFG review committee. 2007-present.

School: Campus:

Student Service: Member, PhD advisory committee for Joshua Blasek, Department of Biology, Purdue School of Science. 2010-present. Member, PhD advisory committee for Rachna Sehgal, Department of Biology, Purdue School of Science. Completed, 2009. Member, PhD advisory committee for Qi Liu, Department of Biology, Indiana University Purdue School of Science. 2006. Member, PhD advisory committee for Hao Zhang, Department of Biochemistry and Molecular Biology, Indiana University School of Medicine. Completed, 2009. Member, PhD dissertation committee for Zhiqian Wang, Department of Cellular & Integrative Physiology, Indiana University School of Medicine. Completed, 2000. Member, PhD dissertation committee for Megan Troxell, Department of Molecular and Cellular Physiology, Stanford University School of Medicine. Completed, 2000. Chairman, PhD dissertation committee for Brad Poteat, Department of Physiology and Biophysics, Indiana University School of Medicine. Member, External member of PhD disertation committee for Da-Ming Li at The University of Illinois at Chicago. Completed, 1996. Chairman, PhD dissertation committee for Kristi Sanborn, Department of Physiology and Biophysics, Indiana University School of Medicine. Judge, Sigma Xi Graduate Student Research Competition, April 1997. Grants and Fellowships: National Institutes of Health, R01. Principal Investigator. Title: Signals regulating SAG development. 2009-2011. Annual direct costs, ave. $397,000. National Institutes of Health, NIAAA Center Grant; PI: David Crabb. Title: Center on Genetic Determinants of Alcohol Ingestion. Pilot Project Director and Collaborator on FAS research section. 2009-2011. Active; annual direct costs, ave. $45,000. National Institutes of Health, R21. Co-Investigator; PI: William Bosron. Title: Effect of ethanol on retinoid metabolism and signaling in zebrafish embryos. 2006-2008. Active; annual direct costs, ave. $125,000. Eli Lilly and Company, Co-Investigator; PI: Bruce Molitoris. Title: Multiphoton imaging program. 2005-2006. Annual direct costs, $30,000.

National Institutes of Health, R01. Principal Investigator. Title: Cadherins in the Developing Zebrafish Inner Ear. 2004-2007. Annual direct costs, ave. $250,000. National Institutes of Health, R01. Principal Investigator. Title: Cell adhesion function in the visual system. 2002-2005. Annual direct costs, ave. $250,000. National Institutes of Health, P50. Pilot Project Director (PI: Bruce Molitoris). Title: Zebrafish as a model to study kidney development (Grant Title: Center for Advanced Renal Microscopic Analysis.) 2002-2004. Annual direct costs, ave. $50,000 Fight For Sight-Prevent Blindness America, Postdoctoral grant to Sherry Babb. Principal Investigator/Mentor. Title: Role of Cadherins in Retinotectal Pathfinding. 2000-2001. Total budget $14,000. National Institutes of Health, P01. Section Director (PI: Bruce Molitoris). Section Title: Junctional complex dysregulation during ischemic injury. 2000-2005. Annual direct costs, ave. $185,000. National Institutes of Health, R01. Principal Investigator. Title: Junctional complex dysregulation during ischemic injury. 1998-2000. Annual direct costs, ave. $151,525. American Heart Association-Indiana Affiliate, Postdoctoral grant to Shobha Gopalakrishnan. Principal Investigator/Mentor. Title: Regulation of tight junction assembly in epithelial cells during renal ischemia. 1997-1999. Total budget $41,000. Showalter Trust Grant. Co-Principal Investigator with Dr. Simon Atkinson. Title: Cellular recovery mechanisms stimulated by growth factor treatment in ischemic acute renal failure. 1997-1998; Total budget $48,617. National Institutes of Health, R29. Principal Investigator. Title: Cell adhesion induced differentiation in eye tissue. 1996-2001. Annual direct costs, ave. $70,000. American Cancer Society, Institutional Grant. Principal Investigator. Title: Cell adhesion induced differentiation in vivo. 1995-1996. Total budget $10,700. American Cancer Society, postdoctoral fellowship, 1994-1995. American Heart Association, postdoctoral fellowship, 1994-1995: declined because of concurrent American Cancer Society award. National Institute of Health, postdoctoral fellowship, 1991-1994. Print and Electronic Publications: Research Articles (Refereed): Marrs, J. A., S. G. Clendenon, D. R. Ratcliffe, S. M. Fielding, Q. Liu and W. F. Bosron. 2010. Zebrafish fetal alcohol syndrome model: effects of ethanol are rescued by retinoic acid supplement. Alcohol. 44: 707-15. Clendenon, S. G., Shah, B., Miller, C. A., Schmeisser, G., Walter, A., Gattone, V. H. Barald, K. F., Liu, Q. and J. A. Marrs. 2009. Cadherin-11 controls otolith assembly: evidence for extracellular cadherin activity. Developmental Dynamics 238: 1909-1922. Lin, F., S. Chen, D. S. Sepich, J. R. Panizzi, S. G. Clendenon, J. A. Marrs, H. E. Hamm, and L. Solnica-Krezel. 2009. Galpha12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton. J. Cell Biol. 184: 909-921. Liu, Q., J. A. Marrs, R. L. Londraville, and A. L. Wilson. 2008. Cadherin-7 function in zebrafish development. Cell Tissue Res. 334: 37-45. Liu, Q., R. Londraville, J. A. Marrs, A. L. Wilson, T. Mbimba, T. Murakami, F. Kubota, W. Zheng, D. G. Fatkins. 2008. Cadherin-6 function in zebrafish retinal development. Dev Neurobiol. 68: 1107-1122. Wilson, A. L., Y.-c. Shen, S.G. Babb-Clendenon, J. Rostedt, B. Liu, K. F. Barald, J. A. Marrs, and Q. Liu. 2007. Cadherin-4 Plays a Role in the Development of Zebrafish Cranial Ganglia and Lateral Line System. Developmental Dynamics 236: 893-902. 7

Liu Q., R. A. Frey, S. G. Babb-Clendenon, B. Liu, J. Francl, A. L. Wilson, J. A. Marrs, and D. L. Stenkamp. 2007. Differential expression of photoreceptor-specific genes in the retina of a zebrafish cadherin2 mutant glass onion and zebrafish cadherin4 morphants. Exp Eye Res. 84:

163-175.

Babb-Clendenon S. G., Y.-c. Shen, Q. Liu, K. E. Turner, M. S. Mills, G. W. Cook, C. A. Miller, V. H. Gattone II, K. F. Barald, and J. A. Marrs. Cadherin-2 participates in the morphogenenesis of the zebrafish inner ear. J. Cell Sci. 119: 5169-5177. S. R. Young, C, Mumaw, J. A. Marrs, and D. G. Skalnik. 2006. Antisense Targeting of CXXC Finger Protein 1 Inhibits Genomic Cytosine Methylation and Primitive Hematopoiesis in Zebrafish. J. Biol. Chem. 281: 37034-37044. Gopalakrishnan, S., M. A. Hallett, S. Atkinson, and J. A. Marrs. 2007. aPKC-Par complex dysfunction and tight junction disassembly in renal epithelial cells during ATP-depletion. Am. J. Physiol. (Cell Physiol.) 292: C1094-C1102. Liu, B., Duff, R. J., Londraville, R. L., Marrs, J. A., Liu Q. 2006. Cloning and expression analysis of cadherin7 in the central nervous system of the embryonic zebrafish. Gene Expr Patterns. 7: 1522. Liu, Q., Duff, R. J., Liu, B., Wilson, A. L., Babb-Clendenon, S. G., Francl, J., Marrs, J. A. 2006. Expression of cadherin10, a type II classic cadherin gene, in the nervous system of the embryonic zebrafish. Gene Expr Patterns. 6: 703-710. Babb, S. G., Kotradi, S. M., Shah, B., Chiappini-Williamson, C., Bell, L. N., Schmeiser, G., Chen, E., Liu, Q. and J. A. Marrs. 2005. Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Developmental Dynamics 233: 930-945. Liu, Q., J. A. Marrs, E. Azodi, A. E. Kerstetter, S. G. Babb, and L. Hashmi. 2004. Differential expression of cadherins in the developing and adult zebrafish olfactory system. J Comp Neurol. 478: 269-281. Babb, S. G. and J. A. Marrs. 2004. E-cadherin regulates cell movements and tissue formation in early zebrafish embryos. Developmental Dynamics 230: 263-277. Kerstetter, A. E., E. Azodi, J. A. Marrs, and Q. Liu. 2004. Cadherin-2 function in the cranial ganglia and lateral line system of developing zebrafish. Developmental Dynamics 230: 137-143. Liu, Q., A. E. Kerstetter, E. Azodi, and J. A. Marrs. 2003. Cadherin-1, -2, and -11 expression and cadherin-2 function in the pectoral limb bud and fin of the developing zebrafish. Developmental Dynamics 228: 734-739. Novince, Z. M., E. Azodi, J. A. Marrs, P. A. Raymond, Q. Liu. 2003. Cadherin expression in the inner ear of developing zebrafish. Gene Expr Patterns. 3: 337-339. Gopalakrishnan, S., M. A. Hallett, S. J. Atkinson and J. A. Marrs. 2003. Differential regulation of junctional complex assembly in renal epithelial cell lines. Am. J. Physiol. Cell Physiol. 285: C102-C111. Gopalakrishnan, S., Dunn, K. W. and J. A. Marrs. 2003. Testing effects of signal transduction pathways on cadherin junctional complex assembly using quantitative image analysis. Methods 30: 218-227. Liu Q, Londraville RL, Azodi E, Babb SG, Chiappini-Williamson C, Marrs JA, Raymond PA. 2002. Up-regulation of cadherin-2 and cadherin-4 in regenerating visual structures of adult zebrafish. Exp. Neurol. 177: 396-406. Gopalakrishnan S, Dunn KW, and Marrs JA. 2002. Rac1, but not RhoA signaling, protects epithelial adherens junction assembly during ATP-depletion. Am. J. Physiol. (Cell Physiol.) 283: C261C272. Liu Q, Babb SG, Novince ZM, Marrs JA, and Raymond PA. 2001. Differential expression of cadherin-2 and cadherin-4 in the developing zebrafish visual system. Visual Neuroscience 18: 923-933.

Liu Q., Marrs JA, Chuang JC, and Raymond PA. 2001. R-cadherin expression in the zebrafish central nervous system and regulation by ventral midline signaling. Brain Res Dev Brain Res. 131: 1729. Babb SG, Barnett J, Doedens AL, Cobb N, Liu Q, Sorkin BC, Yelick PC, Raymond PA, and Marrs JA. 2001. Zebrafish E-cadherin: phylogenic analysis, expression during early embryogenesis and regulation during brain development. Developmental Dynamics 221: 231-237. Jin Y, Atkinson SJ, Marrs JA, Gallagher PJ. 2001. Myosin II light chain phosphorylation regulates membrane localization and apoptotic signaling of tumor necrosis factor receptor-1. J. Biol. Chem. 276: 30342-30349. Troxell, ML, Loftus DJ, Nelson WJ, and Marrs JA. 2001. Mutant cadherin effects on epithelial tubulogenesis, invasion, and transformation. J. Cell Sci. 114: 1237-1246. Troxell MT, Gopalakrishnan S, McCormack J, Poteat BA, Pennington J, Garringer SM, Schneeberger EE, Nelson WJ, Marrs JA. 2000. Inhibiting cadherin function by dominant mutant E-cadherin expression increases the extent of tight junction assembly. J. Cell Sci. 113: 985-996. Liu, Q, Sanborn KL, Cobb N, Raymond PA, Marrs JA. 1999. R-cadherin expression in the developing and adult zebrafish visual system. J. Comp. Neurol. 410: 303-319. Liu, Q, Marrs JA, Raymond PA. 1999. Spatial correspondence between R-cadherin expression domains and retinal ganglion cell axons in developing zebrafish. J. Comp. Neurol. 410: 290302. Troxell MT, Chen Y-T, Cobb N, Nelson WJ, Marrs JA. 1999. Cadherin function in junctional complex rearrangement and posttranslational control of cadherin expression. Am. J. Physiol. 276 (Cell Physiol.45): C404-C418. Gopalakrishnan, S., N. Raman, S. J. Atkinson and J. A. Marrs. 1998. Rho GTPase signaling regulates normal tight junction assembly and protects tight junctions from disassembly during cellular injury. Am. J. Physiol. 275 (Cell Physiol.44): C798-C809. Jou, T.-S., D. B. Stewart, J. Stappart, W. J. Nelson, and J. A. Marrs. 1995. Genetic and biochemical dissection of protein linkages in the cadherin-catenin complex. Proc. Natl. Acad. Sci. USA 92: 5067-5071. Marrs, J. A., C. Anderson-Fisone, M. C. Jeong, I. R. Nabi, C. Zurzolo, E. Rodriguez-Boulan, and W. J. Nelson. 1995. Plasticity in Epithelial Cell Phenotype: Modulation by Expression of Different Cadherin Cell Adhesion Molecules. J. Cell Biol. 129: 507-519. Marrs, J. A., E. W. Napolitano, C. Murphy-Erdosh, R. W. Mays, L. F. Reichardt and W. J. Nelson. 1993. Distinguishing roles of the membrane-cytoskeleton and cadherin mediated cell-cell adhesion in generating different Na+, K+-ATPase distributions in different polarized epithelia. J. Cell Biol. 123: 149-164. Marrs, J. A., and G. B. Bouck. 1992. The two major membrane skeletal proteins (articulins) of Euglena gracilis define a novel class of cytoskeletal proteins. J. Cell Biol. 118: 1465-1475. Rosiere, T. K., J. A. Marrs, and G. B. Bouck. 1989. A 39-kD plasma membrane protein (IP39) is an anchor for the unusual membrane skeleton of Euglena gracilis. J. Cell Biol. 110: 1077-1088. Review Articles: Marrs, J. A. 2010. Branching morphogenesis: Rac signaling PIX tubulogenesis. Focus on Pak1 regulates branching morphogenesis in 3D MDCK cell culture by a PIX and beta-1-integrindependent mechanism. Am. J. Physiol. Cell Physiol. (Epub ahead of print, Apr 28.). Marrs, J. A. and Liu, Q. 2010. Cadherin adhesion functions revealed in zebrafish. In: K. Yoshida, Ed. Molecular and Functional Diversities of Cadherin/Protocadherin. Research Signpost, Kerala, India. In Press. Marrs, J. A., Gopalakrishnan, S., and R. L. Bacallao. 2001. Tight junction and adherens junction dysfunction during ischemic injury. In: B. A. Molitoris, B. A. and Finn, W., Eds. Acute Renal

Failure: A companion to Brenner and Rector's The Kidney. W. B. Saunders Company, Philadelphia PA. pp. 132-142. Marrs, J. A. and B. A. Molitoris. 2001. Ischemia-induced tight junction dysfunction in the kidney. In: Cereijido, M. and Anderson, J. Eds. Tight junctions, Second Edition. CRC Press, Boca Ratton, FL. pp. 533-51. Molitoris, B. A., and J. A. Marrs. 1999. The role of cell adhesion molecules in ischemic acute renal failure. American Journal of Medicine. 106: 583-592. Marrs, J. A., and W. J. Nelson. 1998. Roles for the membrane-cytoskeleton in generating and maintaining epithelial cell polarity. In Principles of Medical Biology, (E. E. Bittar, Ed.) JAI Press, Greenwich CT. Volume 11 pp. 69-87. Marrs, J. A., and W. J. Nelson. 1996. Cadherin Cell Adhesion Molecules in Differentiation and Embryogenesis. Int. Rev. Cytol. 165: 159-205. Mays, R. W., W. J. Nelson, and J. A. Marrs. 1996. Generation of epithelial cell polarity: roles for protein trafficking, membrane-cytoskeleton, and E-cadherin-mediated cell adhesion. Cold Spring Harbor Symp. Quant. Biol. 60: 763-773. Marrs, J. A., and W. J. Nelson. 1994. Determinants of cell surface distributions of Na+, K+-ATPase in polarized epithelial cells. In The Sodium Pump. E. Bamburg and W. Schoner, eds. Darmstadt, Steinkopff, New York, NY. pp. 159-169. Fazio, M. J., J. A. Marrs, and G. B. Bouck. 1994. Protein kinases in protists. Protoplasma 181: 283290. Dubreuil, R. R., J. A. Marrs, and G. B. Bouck. 1992. The cytoskeleton in euglenoids: cell form, surface motility and cell replication are based on a membrane skeleton of repeating strips. In The Cytoskeleton of the Algae. D. Menzel, ed. CRC Press, Boca Ratton, FL. pp. 59-78.

10

Curriculum vitae
KATHLEEN A. MARRS, Ph.D. Associate Professor of Biology Associate Dean for Academic Affairs IUPUI Department of Biology 723 W. Michigan Street Indianapolis IN 46202-3273 Phone: (317) 278-4551 Fax: (317) 274-2846 e-mail: kmarrs@iupui.edu

ACADEMIC APPOINTMENTS 2009 - Present Associate Dean for Academic Affairs, School of Science, IUPUI 2009 - Present Director of the Woodrow Wilson Indiana Teaching Fellowship program at IUPUI 2009 - Present Director of UCASE, the Urban Center for the Advancement of STEM Education 2006 - 2009 Assistant Director of UCASE, the Urban Center for the Advancement of STEM Education, IUPUI School of Science and Indiana University School of Education 2004 - Present Associate Professor of Biology, Department of Biology, IUPUI 2001 - Present Adjunct Assistant Professor, University College, IUPUI 1998 - 2004 Assistant Professor of Biology, Department of Biology, IUPUI 1996 - 1998 Adjunct Assistant Professor, Biology Department, University of Indianapolis PROFESSIONAL PREPARATION 1993 - 1995 Stanford University, Palo Alto CA 1991 - 1993 Sandoz Agro Biotechnology, Palo Alto CA 1985 - 1990 University of Illinois at Chicago, Chicago IL 1980 - 1984 Illinois Wesleyan University, Bloomington IL

Postdoctoral Fellowship Postdoctoral Fellowship Ph.D. Biological Sciences B.A. Biology

HONORS AND AWARDS 2008 IUPUI Chancellors Award for Excellence in Teaching 2008 Excellence in Teaching Award, National Society of Leadership and Success 2006 IUPUI School of Science Trustees Teaching Award 2005 IUPUI Gateway Scholar, Office for Professional Development 2003 Faculty Colloquium on Excellence in Teaching (FACET) inductee 2003 John A. Curtis Lecture Award, American Society for Engineering Education 2002 IUPUI School of Science Trustees Teaching Award 2001 Project Kaleidoscope 21st Century Faculty Member 2001 IUPUI School of Science Teaching Award 2001 Golden Key, Honorary Inductee (Nominated by IUPUI Students) 2000 Project Kaleidoscope "Leader in Undergraduate STEM Education" 1999 IUPUI Network for Excellence in Teaching (NET) Award 1993 to 1994 Postdoctoral Fellowship: NSF/USDA/DOE Tri-Agency Program 1989 to 1990 Graduate Student Fellowship: University of Illinois at Chicago 1989 Cold Spring Harbor Laboratory Scholarship

Curriculum vitae
GRANTS AWARDED 1. Midwest Noyce Regional Conference at IUPUI (2010-2011) Principal Investigator: Kim Nguyen, Co-PIs: Kathleen Marrs, John Staver, and Signe Kastberg (NSF DUE #1002638) $353,300 2. Noyce Summer Internship Program at IUPUI (2009-2011) Kathleen Marrs, Co-PIs: Kim Nguyen, Dwight Schuster Supplement to Advance Urban Learning: Teach Science (NSF DUE # 0733788-02) $72,000. 3. Strategies for Advancing Math and Science Teaching in High Need Schools: The Midwest Regional Robert Noyce Conference (2008-2009) Kathleen Marrs and Kim Nguyen, Supplement to Advance Urban Learning: Teach Science (NSF DUE # 0733788-01) $90,000. 4. Urban Educators: Robert Noyce Scholarship for Mathematics and Science Teachers, Phase II (2009-2012) Principal Investigator: Kim Nguyen, Co-PIs: Kathleen A. Marrs, Signe Kastberg, (NSF DUE # 0934555) $599,993 5. The GK-12 Urban Educators Program at IUPUI (2008-2012) Principal Investigator: Kathleen A. Marrs, Co-PIs: Simon Rhodes, Pam Crowell, Lenore Tedesco, Andy Gavrin (NSF DUE # 0742475) $2,988,355 6. Advance Urban Learning: Teach Science (2007-2011) Principal Investigator: Kathleen A. Marrs, Co-PIs: Dwight Schuster, and Kim Nguyen (NSF DUE # 0733788) $749,933 7. Urban Educators: Robert Noyce Scholarship for Mathematics and Science Teachers (20052008) Principal Investigator: Charlie Barman, Co-PIs: Kathleen A. Marrs, Signe Kastberg, and Kim Nguyen (NSF DUE # 0531598) $486,912 8. Project SEAM, A Joint Venture for Collaborative Professional Development focused on Core Academic Student Preparation for Post-Secondary Success (2000-2004), Lilly Endowment, Indianapolis IN, Biology Committee Chair $6,615,000 (Biology $105,000) 9. WebScience: Creating an Active-Learner Classroom with WWW Technology to Improve Introductory Science and Mathematics Courses. (1999-2002), (NSF-DUE #9981111), PI: Andrew Gavrin, Co-PIs: Kathleen A. Marrs, Jeffrey Watt, Robert Blake. $141,500. PEER-REVIEWED PRINT AND ELECTRONIC PUBLICATIONS Peer-reviewed Publications While in Rank 1. Allen JR, Buckingham JE, Roper RJ, Marrs KA (2010) How Middle School Students Come Face to Face with Down Syndrome Research. Science Scope, 33:20-25, January 2010 2. Marrs KA (2009) Just-in-Time Teaching in the Biological Sciences; Simkins, S.P. & Maier, M.H. (Eds.). Just-in-Time Teaching Across the Disciplines. p. 81-99. Sterling, VA: Stylus Publishing LLC 3. Marrs KA and Chism GW III (2005) Just-in-Time Teaching for Food Science: Creating an Active Learner Classroom. Journal OF Food Science Education, 4:27-34. 4. Rutledge, M., Sadler, K., and Marrs, K.A (2005) Instructors Resource Guide for Biology: Understanding Life (Alters, Sandra and Brian), John Wiley and Sons, Hoboken NJ. 5. Marrs KA (2004). Design and Construction of a Prototype Just-in-Time Teaching Digital Library Metadata Scheme Linking Fundamental Concepts in Biology with Pedagogical

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Retrieval Tools. Electronic publication for national launch of JiTTDL: The Just-in-Time Teaching Digital Library (NSF-DUE-0333646) 6. Marrs KA (2004) How do you know what your students know? Determining Student Prior Knowledge Using Web-based Warm Up Exercises. in: Quick Hits For New Faculty: Teaching Strategies Aimed At First-Time Faculty, Indiana University Press, Bloomington IN, p 67-70. 7. Gavrin A., Watt JX, Marrs KA, and Blake RE, (2004) Using the Web to Enhance Classroom Learning with Just-in-Time Teaching. Computers in Education Journal, Vol. XIV (2)51-60. 8. Marrs KA and Novak G (2004) Just-in-Time Teaching in Biology: Creating an Active Learner Classroom Using the Internet Cell Biology Education, 3:49-61. 9. Marrs KA (2004) Determining Student Prior Knowledge and Misconceptions using Web-based Warm Up Exercises. In: Teaching Tips: Innovations in Undergraduate Science Instruction. Eds. Marvin Druger and Linda Crow, Society for College Science Teachers SCST Monographs, Special 25th Anniversary Issue, ISBN: 0873552458. 10. Gavrin AD, Watt JX, Marrs KA and Blake RE (2003) Just-in-Time teaching (JiTT): Using the Web to Enhance Classroom Learning. Proceedings of the 2003 American Society for Engineering Education 3220:1-13. 11. Marrs KA, Gilman S, Rhoton J (2003) NSTA Position Statement: The Importance of Teaching in Tenure and Promotion. Date of Adoption by NSTA: March 28, 2003. 12. Marrs KA, Blake RE and Gavrin AD (2003) Use of Internet Based Warm Up Exercises to Determine Students Prior Knowledge and Misconceptions in Biology, Chemistry, and Physics. Journal of College Science Teaching, 33(1):42-47. Peer-Reviewed Publications Prior to Rank 1. Marrs KA and Walbot V (1997) Expression and RNA splicing of the maize glutathione Stransferase Bronze2 gene is regulated by cadmium and other stresses. Plant Physiology, 113:93-102. 2. Marrs KA and Sinibaldi RM (1997) A heat-inducible and developmentally regulated DNAbinding factor interacts with conserved promoter regions of the maize hsp82, hsp81, and hsp17.9 gene families. Maydica, 42:211-216. 3. Marrs KA (1996) The functions and regulation of Glutathione S-transferases in plants. In: Annual Review of Plant Physiology and Plant Molecular Biology 47:127-158. ed. Russell Jones. Annual Reviews Inc., Palo Alto CA. 4. Marrs KA, Alfenito MR, Lloyd AM, and Walbot V (1995) The maize Bronze-2 encodes a glutathione S-transferase involved in the transfer of anthocyanins to the vacuole. Nature, 375:397-400. 5. Marrs KA, Murry L, and Rhodes CA (1995) Transformation of maize by electroporation of embryos. In: Protocols for Electroporation and Electrofusion of Plant and Animal Cells 55:121131. ed. Jac A. Nickoloff. Harvard Press, Cambridge MA.

Curriculum vitae
6. Marrs KA and Urioste J (1995) Transient gene expression analysis in electroporated maize protoplasts. In: Protocols for Electroporation and Electrofusion of Plant and Animal Cells 55:133145. ed. Jac A. Nickoloff. Harvard Press, Cambridge MA. 7. Urioste J, Marrs KA, Bodeau J, and Walbot V (1995) Gene transfer to protoplasts: transient expression for plasmid analysis. Invited review in: Gene Transfer to Plants. ed. I. Potrykus. Springer-Verlag. 8. Marrs KA, Casey ES, Capitant SA, Bouchard RA, Dietrich PS, Mettler IJ, and Sinibaldi RM (1993) Characterization of two maize HSP90 heat shock protein genes: expression during heat shock, embryogenesis, and pollen development. Dev. Genet., 14:27-41. 9. Kaufman LS, Gao G, Bhattacharya K, Tilghman J, Marsh J, Marrs KA, and Warpeha KMF (1993) Blue light regulation of Cab gene expression in the apical buds of peas and the cotyledons of Arabidopsis. In: Frontiers of Photobiology, pp 37-39. eds. A. Shima, M. Ichahashi, Y. Tujiwara and H. Takebe. Exerpta Medica, Amsterdam. 10. Kaufman LS, Marrs KA, Warpeha KMF, Gao G, Bhattacharya K, Tilghman J, and Marsh JF (1992) Blue-light regulated gene expression. In: Cellular Communication in Plants, pp 21-26.. ed. R. Amasino. Plenum, NY. 11. Marrs KA and Kaufman LS (1991) Rapid transcriptional regulation of the Cab and pEA207 gene families by blue light in the absence of protein synthesis. Planta, 183: 327-333. 12. Warpeha KMF, Marrs KA, and Kaufman LS (1990) Effects of blue light on plant growth. Section: Plant Growth. In: McGraw Hill Yearbook of Science and Technology, pp. 310-311. McGraw Hill, New York, NY. 13. Warpeha KMF, Marrs KA, and Kaufman LS (1989) Blue-light regulation of specific transcripts in Pisum sativum: fluence-response, time course, and reciprocity characteristics. Plant Physiol., 91: 1030-1035. 14. Marrs KA and Kaufman LS (1989) Blue-light regulation of transcription for nuclear-encoded genes in pea. Proc. Natl. Acad. Sci., 86:4492-4495. PRESENTATIONS Invited Presentations and Workshops Given While In Rank 1. Plenary Panel: Bringing Research to K-12 Classrooms: The Fellow, Teacher, PI Team (2010) Nikki Duvall, Joshua Blazek, Beverly Ransdell, Kathleen Marrs, NSF GK-12 National Meeting, Washington DC 2. The GK-12 Program: The Benefits of Having a Graduate Student in your Classroom HASTI (Hoosier Association of Science Teachers) Meeting, Indianapolis IN February 2010 3. Algal Bloom Investigation (2010) Angela M. Cowan, Brooke Furge, Lenore P. Tedesco, Kathleen A. Marrs, AAAS, San Diego CA (Poster) 4. Bringing Down Syndrome Research Into the Classroom (2009) Jared Allen and Kathy Marrs, GK-12 National Meeting, Washington DC March 2009 5. The Woodrow Wilson Indiana Teaching Fellowship (2009) Connie Bond, Kim Nguyen, Kathleen Marrs, National Science Foundation Robert Noyce Annual Meeting, Washington DC
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6. Rethinking Teacher Education: The Woodrow Wilson Indiana Teaching Fellowship Model (2009) James Fraser, Sidney Moon, James Lehman, Kathleen Marrs, Jennifer Drake, Angela Ridgway, Laurie Mullen, The American Association of Colleges for Teacher Education (AACTE) Annual Meeting, Chicago IL 7. Peer-led Team Learning: Preparing Talented Science Majors for Careers in Teaching (2009) Pratibha Varma-Nelson, Jessica Blackerby, Chad Higgenbotham, Kathleen Marrs, Midwest Noyce Conference: Strategies for Advancing Math and Science Teaching in High Need Schools, Indianapolis IN 8. Using Grad Students to Further STEM Education: The IUPUI GK-12 Urban Educators Program (2009) Diedre Adams, Jared Allen, Jane Buckingham, Kathy Marrs. The Indiana Conference on Learning, Indianapolis IN 9. IUPUI Partnerships in STEM Education: Workforce Development for Scientists and Teachers (2008) Kathleen A. Marrs, IUPUI / IU Foundation Community Luncheon - Building a Workforce for Tomorrow through Science, Technology, Engineering and Mathematics Education, Indianapolis IN 10. Student Learning and College Science Teaching (2008) Marrs KA Department of Biology, The University of Colorado Denver, Denver CO 11. Just in Time Teaching: a Research-Based Approach for Re-inventing Undergraduate Science Education (2008) Marrs KA, Department of Plant Biology, Division of Science and Mathematics Education, Michigan State University, East Lansing MI 12. In Pursuit of Meaningful College Science Teaching and Learning (2008) Kathleen A. Marrs, The Center for Research on College Science Teaching and Learning (CRCSTL), Michigan State University, East Lansing MI 13. Just in Time Teaching: a Research-Based Approach to Enhancing Undergraduate Student Success in Science (2008) Kathleen A. Marrs, Department of Biochemistry, Cellular, and Molecular Biology, The University of Tennessee Knoxville, Knoxville TN 14. From My Course to Our Curriculum: Integrating ePortfolio into the Major (2008) Susan Kahn, Kathleen A. Marrs, Michelle Roberts, Lee Vander Kooi (Workshop; Session 03C) The Assessment Institute of Indianapolis, Indianapolis IN 15. IUPUIs Transition to Teaching Program: Preparing Teachers for the 21st Century (2008) Charles Barman, Kathleen Allspaw, Linda Houser, Kathleen A. Marrs, Rebecca Schini, Kylee List, C. Layton Elliott, Brad Hess, The Hoosier Association of Science Teacher (HASTI) Annual Meeting, Indianapolis IN 16. So You Think They Got It? Measuring Student Learning in Ways That Matter (2007) Kathleen Marrs, Crystal Garcia, and Jennifer Beasley, Symposium on First-Year Student Success, Gateway to Graduation Initiative, IUPUI Center for Teaching and Learning 17. Biotechnology 101: From Cloning to Stem Cells (2007) Kathleen A. Marrs, Indiana Health Industry Forum (IHIF) Innovention 2007 Symposium

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18. Just-in-Time Teaching Workshop for the Boston Science Partnership (2007) Kathleen A. Marrs, Contextualized Content Courses (CCC) Instructors Workshop, University of Massachusetts Boston 19. Implementing the Seven Principles for Good Practice in Undergraduate Education (2006) Keith Anliker, Herbert Brant, Crystal Garcia, Bill Gronfein, Greg Keller, Kathleen Marrs, Melinda Philabaum, and Kate Thedwell. Gateway Scholars Workshop, Edward C. Moore Teaching Symposium 2006. Mission: Transformation of College Teaching; Challenge: How can we get there? IUPUI, Indianapolis IN 20. Just in Time Teaching: Strategies for Student Success (2006) Kathleen A. Marrs, BenjaminCummings Strategies for Success Workshop, IUPUI Indianapolis, IN 21. Just-in-Time Teaching: Using the Web as a Tool to Transform Teaching and Learning, and Advance the Scholarship of Teaching and Learning. (2004) Kathleen A. Marrs, The International Society for the Scholarship of Teaching and Learning, Indiana University, Bloomington IN. 22. Just-in-Time Teaching: Creating a Classroom-tested, Research-based Approach for Teaching and Learning Science (2004) NSF Chautauqua Workshop, US Air Force Academy, Colorado Springs CO (Workshop Co-organizer with Gregor Novak, Evelyn Patterson, and Jim Benedict) 23. Developing a Just-in-Time Teaching Approach for Teaching and Learning (2004) Kathleen A. Marrs and Andrew D. Gavrin, Faculty Development Conference, Indiana University Purdue University Fort Wayne (IPFW) Center for Enhancement of Teaching and Learning (CELT) 24. Just-In-Time Teaching: Creating an Interactive, Web-Based Course to Improve Student Learning and Classroom Performance. (2003) Kathleen A. Marrs and Andrew Gavrin, FACET Associate Faculty and Lecturer Conference, Indianapolis IN 25. Just-In-Time Teaching: Creating an Interactive, Web-Based Course to Assess Student Knowledge, Misconceptions, and Classroom Performance. (2003) Kathleen A. Marrs and Andrew Gavrin, The 2003 National Assessment Institute, Best Practices in Assessment, IUPUI, Indianapolis IN 26. JiTT: Using the Internet to Identify Student Misconceptions and Create an Active-Learner Classroom in Biology (2003) Marion College, Fall Seminar Series 27. Just-in-Time Teaching: Creating an Active-Learner Classroom using the Web to Improve Learning in Introductory Biology Courses (2003) NSF Chautauqua Workshop US Air Force Academy, Colorado Springs CO (Workshop Co-organizer with Gregor Novak, Evelyn Patterson, and Virginia Mathie) 28. Just-in-Time Teaching in Biology: Blending Active Learning with Internet Technology (2002) IUPUI Biology Department Seminar, Indianapolis IN 29. Just-in-Time Teaching: Using the Web as a Vehicle to Blend Active Learning and Instruction; or: What if students dont live for your course? (2002) Indiana University Purdue University Fort Wayne (IPFW), Fort Wayne IN.

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30. Just-in-Time Teaching: Using the Web as a Vehicle to Blend Active Learning and Instruction (2002) Kathleen A. Marrs and Robert Blake, National Science Teacher Association Meeting, San Diego 31. Using Just in Time Teaching To Determine Prior Knowledge and Assess Student Learning in Biology, Physics and Math (2002) Kathleen A. Marrs, Andrew D. Gavrin, and Jeffrey X. Watt, The 2002 National Assessment Institute, Best Practices in Assessment, Indianapolis IN 32. Just-in-Time Teaching: Using the Web to Identify Student Misconceptions in Biology, Chemistry, and Physics (2002) Kathleen A. Marrs Andrew Gavrin, and Robert Blake, National Science Teacher Association Meeting, San Diego 33. Just-in-Time Teaching: Creating an Interactive, Classroom-tested, Research-based Approach for Teaching and Learning in Introductory Science Courses (2002) NSF Chautauqua Workshop US Air Force Academy, Colorado Springs CO (Workshop Co-organizer with Gregor Novak, Evelyn Patterson, and Jim Benedict) 34. WebScience: Blending Active Learning with World Wide Web Technology (2001) Kathleen A. Marrs and Andrew D. Gavrin, Hoosier Association of Science Teachers (HASTI) 31st Annual Convention, Indianapolis IN 35. Project Seam: Bridging the Gap Between High School and College (2001) Gary L. Cooper, David Dowell, Todd W. Murphy, Elizabeth Thoas, Andrea P. Stonebreaker, Steve Bair, and Kathleen A. Marrs, Hoosier Association of Science Teachers (HASTI) 31st Annual Convention, Indianapolis IN 36. WebScience: The Use of the World Wide Web as a Vehicle for the Teaching and Learning of Biology (October 2001) Kathleen A. Marrs, Andrew D. Gavrin, and Jeff Watt, EduCause Conference, Indianapolis IN 37. Preparing Students for Other Worlds: The Integration and Application of a College Education. (2001) Kathleen A. Marrs, Anna Musick, Charles Barman, and Richard E. Ward, Edward C. Moore Teaching Symposium 2001: Strategizing for Learning: The Role of Outcomes in University Education, IUPUI, Indianapolis IN 38. Engaging Students in Large Classes: It Can be Done! (2000) Kathleen A. Marrs, Andrew D. Gavrin, and Nancy Chism, Edward C. Moore Teaching Symposium 2000: Pedagogies of Engagement Teaching for Deeper Understanding, IUPUI, Indianapolis IN 39. Just-in-Time Teaching: Creating an Active-Learner Classroom using the World Wide Web to Improve Learning in Introductory Biology Courses (2000) Kathleen A. Marrs, Project Kaleidoscope 2000 Summer Institute, Keystone, CO 40. Project Seam, a Joint Venture Focused on Core Academic Student Preparation for PostSecondary Success (2000) Gary L. Cooper, David Dowell, Todd W. Murphy, Elizabeth Thoas, Andrea P. Stonebreaker, Steve Bair, and Kathleen A. Marrs, Annual Meeting of the National Association of Biology Teachers (NABT), Orlando FL Presentations Prior to Rank 1. The Maize Bronze-2 Gene: A Colorful Tale of GSTSs and their Natural Substrates. (1997) International Workshop on Glutathione Transferases, Rome, Italy.

Curriculum vitae
2. The Maize Bronze-2 Gene Encodes a Glutathione S-transferase: an Essential Step in Localization of Anthocyanins to the Vacuole. (1994) Department of Botany and Plant Pathology, Purdue University, West Lafayette IN. 3. The Maize Bronze-2 Gene Encodes a Glutathione S-transferase that Localizes Anthocyanins to the Vacuole. (1994) Department of Plant Biotechnology, DowElanco, Zionsville IN.

4. Life in an Uncertain World: the Impact of Environmental Stress on Gene Expression and RNA Splicing in Maize. (1994) Department of Biology, Indiana University Purdue University at Indianapolis, Indianapolis IN. 5. Bronze-2 gene expression and RNA splicing are regulated by environmental stress. (1994) Maize Genetics Meeting, St. Charles IL. 6. The Impact of Heat Shock and Heavy Metal Stress on Gene Expression and RNA Splicing in Maize. (1994) Department of Biological Sciences, University of Louisville, Louisville KY. 7. The Impact of Environmental Stress on Gene Expression and RNA Splicing in Maize. (1993) Department of Plant Biology, Carnegie Institution of Washington, Stanford CA. 8. Life in an Uncertain World: the Impact of Environmental Stress on Gene Expression and RNA Splicing in Maize. (1993) Department of Agronomy, University of Illinois, Urbana IL. 9. Regulation of Maize 82kD and 18kD Heat Shock Gene Transcription by Sequence-Specific DNA Binding Proteins. (1992) Monsanto Corp, St Louis MO. 10. Challenges and Problems Associated with the Expression of Transgenes in Plants. (1992) Molecular Crop Agriculture for the Pacific Rim, Sacramento CA. 11. Transcriptional Regulation of Nuclear-Encoded Genes in Pisum sativum by Blue Light. (1991) Sandoz Crop Protection Corp, Palo Alto CA. 12. Blue Light Regulation of Nuclear Gene Expression in Pisum sativum. (1989) American Society of Plant Physiologists, Toronto Canada. 13. Transcriptional and Post-transcriptional Regulation of Gene Expression by Blue Light in Pea. (1989) Midwest Section of the American Society of Plant Physiologists, DeKalb IL. 14. Cryptochrome Regulation of Nuclear Gene Transcription in Pea. (1988) American Society of Plant Physiologists, Reno NV. 15. Cryptochrome Regulated Nuclear Gene Expression in Pisum sativum cv. Alaska. (1987) Midwest Section of the American Society of Plant Physiologists, DeKalb IL. PROFESSIONAL ORGANIZATIONS 2001 to present 2000 to present 2000 to present 1999 to present Indiana Academy of Science Project Kaleidoscope (PKAL) Hoosier Association of Science Teachers (HASTI) National Science Teachers Association (NSTA)
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Curriculum vitae
1999 to present 1998 to present Society of College Science Teachers (SCST) National Association of Biology Teachers (NABT)

Curriculum vitae
TEACHING AND INSTRUCTIONAL EFFECTIVENESS List of Courses Taught and Global Scores of Student Satisfaction (5 point scale): Contemporary Biology (Biology N100): (3 cr.) [1998-2003] Selected principles of biology with emphasis on issues and problems extending into everyday lives of the students taught using Just in Time Teaching (JiTT) methodology. (http://www.biology.iupui.edu/biocourses/N100/). Enrollment = ~400 students per year. Global Scores = 4.50 or above. Topics in Biotechnology (Biology 54000): (3 cr) [1998-Present] This Graduate course explores research techniques, applications and legal/ethical issues raised by biotechnology and recombinant DNA technology. Each year, the course content changes significantly to reflect the advances made in Biotechnology. (http://www.biology.iupui.edu/biocourses/biol540/) Enrollment = ~60 students per fall semester. Global Scores = 4.70 or above. Genetics Laboratory (Biology K323): (3 cr.) [1999-Present] Applied principles of genetics using model genetic organisms such as viruses, yeasts and Drosophila. Includes special projects for Honors students. Enrollment = 90 students per fall semester; 5 -10 of these students do an Independent Honors research project under my supervision. Global Scores = 4.60 or above. Cell Biology Laboratory (Biology K325): (3 cr) [1999-Present] Experiments on the molecular and biochemical basis of organization and function of the eukaryotic cell. Includes special projects for Honors students. Enrollment = 90 students per semester; 5 -10 of these students do an Independent Honors research project. Global Scores = 4.60 or above. Microbiology Laboratory (Biology K357): (3 cr) [1999-Present] Laboratory exercises to develop proficiency in aseptic cultivation and utilization of microorganisms, plus experimental investigations of microorganisms. Includes special projects for Honors students within each section. Enrollment = 90 students per fall semester; 5 -10 of these students do an Independent Honors research project. Global Scores = 4.60 or above. Concepts of Biology (Biology K101): (5 cr) [2004-present] This is the largest Majors course in the School of Science, with a yearly enrollment of 800 students. This course is taught using Just in Time Teaching (JiTT) (http://www.biology.iupui.edu/biocourses/k101/). Lecture plus supervision of 20 laboratory sections (graduate student TAs), and 20 recitation sections (undergraduate peer mentors). Approximately 24 K101 students take a separate honors section and do an Independent Honors research project. Global Scores = 4.40 or above. The Nature of Science and the Stem Disciplines (Educ S502): (3 cr) [2009-present] This is the first course for graduate students in the Woodrow Wilson Indian Teaching Fellowship, a selective statewide program for students seeking a masters degree and seeking secondary STEM teacher certification. Enrollment = 20 students per summer session. In addition, each semester I supervise ~20 students each year doing their senior capstone research project or paper (K490, K493, K494). Ability to Motivate Students - IUPUI Mentoring Citations: I have been given the following mentoring citations from graduating IUPUI seniors since 2001: 56 Level 1 citations (Remarkable and Positive Impact), 89 Level 2 citations (Remarkable and Positive - One of the Top 3), 46 Level 3 citations (Influenced the Course of My Life)

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Curriculum vitae
PROFESSIONAL AND UNIVERSITY SERVICE: (Italics = past service on this committee) Departmental: Curriculum Committee, Undergraduate Recruitment Committee, IUPUI Biology Club Advisor (Since 1999), IUPUI Pre-Pharmacy Club Advisor (Since 2005) School of Science: Educational Policies (Chair, Deans Office Liason), Assessment Committee (Deans Office Liason), Technology Committee (Chair), Nominations and Awards (Deans Office Liason), The Leadership Collaborative of the Science and Mathematics Teacher Imperative (TLC-SMTI), Association of Public and Land-Grant Universities (APLU), (IUPUI Team Leader) IUPUI Campus: Academic Affairs Committee AAC (Chair); Academic Policies and Procedures Committee (APPC); Undergraduate Curriculum Advisory Committee (UCAC) (Chair), P-20+ Talent Council, CRL (Center for Research and Learning) Leadership Team, 2012 Committee: (Accreditation), Faculty Council IU-System Wide: Educational Policies (Co-Chair) CURRENT SYNERGISTIC ACTIVITIES 1. Director of UCASE, The Urban Center for the Advancement of STEM Education Coordinate undergraduate Robert Noyce Teach Science Scholarship and Internship programs Oversee recruitment, project assessment, internships for undergraduate science majors 2. Director of Woodrow Wilson Indiana Teaching Fellowship program at IUPUI Coordinate Masters Degree program for 20 STEM students / year Oversee curriculum, project assessment, mentoring, and collaborate in statewide partnership 3. Principal Investigator of the IUPUI Urban Educators GK-12 Program at IUPUI (NSF-DGE) Coordinate 11 GK-12 Graduate Fellows / year working with local teachers grades 6-12 Themes: Medicine and Human Health and Discovering the Science of the Environment 4. Principal Investigator of the IUPUI Robert Noyce Teach Science Program (NSF-DUE) Coordinate scholarships for IUPUI students to become secondary STEM teachers Coordinate internships for IUPUI undergraduates interested in informal Science Education 5. The Leadership Collaborative of the Science and Mathematics Teacher Imperative (TLC-SMTI), Association of Public and Land-Grant Universities (APLU) IUPUI Team Leader of national effort to increase quality and quantity of STEM teachers Development of Implementation & Assessment Plan (IAP) for IUPUI Campus (Dec 2009) 6. Associate Dean for Academic Affairs, IUPUI School of Science Engage the faculty in curricular innovations to increase school-wide offerings of honors courses, dual major / dual degree programs, and interdisciplinary programs for science majors Increase the quality of the entering students, support and extend academic advising services, enhance the diversity of the student body, and improve the 4-year graduation rate. POSTDOCTORAL AND PH.D. THESIS ADVISORS Dr. Virginia Walbot, Department of Biological Sciences. Stanford University Dr. Ralph M. Sinibaldi, Operon Technologies, Inc. Dr. Lon S. Kaufman, Department of Biological Sciences, University of Illinois at Chicago A list of references is available upon request.
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Jason S. Meyer Curriculum Vita November 2010

Jason S. Meyer
Department of Biology, SL306 723 West Michigan Street Indianapolis, IN 46202 http://www.biology.iupui.edu/faculty/jmeyer.html Office Phone: (317) 274-1040 Home Phone: (608) 770-5464 Fax: (317) 274-2846 Email: meyerjas@iupui.edu

Education
1999 - 2004 1994 - 1998 Ph.D., Biological Sciences, University of Missouri-Columbia Field of Study: Neuroscience Advisor: Mark D. Kirk, Ph.D. B.A., Colgate University Major: Biology, Minor: Education

Professional Experience
2010-present Assistant Professor, Department of Biology, Indiana University Purdue University Indianapolis 2008-2010 Assistant Scientist, Waisman Center, Stem Cell Research Program, University of Wisconsin-Madison 2005-2008 Postdoctoral Research Associate, Waisman Center, Stem Cell Research Program, University of Wisconsin

Research Publications
Meyer JS, Howden S, Verhoeven A, Wright LS, Wallace KA, Shearer RL, Capowski E, Thomson JA, and Gamm DM, Induced Pluripotent Stem Cell-Based Phenotypic Correction of Disease Pathology from a Patient With Gyrate Atrophy, in preparation. Meyer JS, Capowski EE, Pinila I, Wallace KA, Wright LS, and Gamm DM, Highly Efficient Differentiation and Enrichment of Forebrain and Retinal Progenitors Derived From Human Embryonic and Induced Pluripotent Stem Cells, in preparation. Meyer JS, Shearer RL, Capowski E, Wright LS, Wallace KA, McMillan EL, Zhang SC, and Gamm DM (2009), Modeling Early Retinal Development with Human Embryonic and Induced Pluripotent Stem Cells, Proc Natl Acad Sci,106(39): 16698-703. Wright LS*, Meyer JS*, Capowski EE, and Gamm DM (2009), Derivation and characterization of human retinal progenitor cells, within Stem Cell Transplantation to the Retina: Development, Plasticity, Regeneration and Repair, Ed. by D. Sakaguchi, H. Klassen, and M. Young. Meyer JS, Tullis GT, Pierret CK, and Kirk MD (2009), Detection of Calcium Transients in Embryonic Stem Cells and Their Differentiated Progeny, Cell Mol Neurobiol, published online 5/28/09, DOI: 10.1007/s10571-009-9413-3.

Jason S. Meyer Curriculum Vita November 2010

Gamm D, Wright LS, Capowski EE, Shearer RL, Meyer JS, Kim HJ, Schneider B, Melvan JN, and Svendsen CN (2008), Regulation of Prenatal Human Retinal Neurosphere Growth and Cell Fate Potential by Retinal Pigment Epithelium and Mash1, Stem Cells 26(12): 3182-93. Zhang ZJ, Meyer JS, and Zhang SC (2007), hES differentiation: Neural cell lineages, within Human Embryonic Stem Cells, Ed. by J. Masters, B. Palsson, and J. Thomson. Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2006), Embryonic stem cell derived neural precursors incorporate into the degenerating retina and enhance survival of host photoreceptors, Stem Cells 24(2): 274-283. Meyer JS, Katz ML, and Kirk MD (2005), Stem Cells for Retinal Degenerative Disorders, Ann NY Acad Sci 1049:135-145. Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2004), Neural differenation of mouse embryonic stem cells in vitro and after transplantation into eyes of mutant mice with rapid retinal degeneration, Brain Res 1014(1):131-144. Kirk MD, Meyer JS, Miller MW, and Govind CK (2001), Dichotomy in Phasic-Tonic Neuromuscular Structure of Crayfish Inhibitory Axons, J Comp Neurol 435: 283-290.

Select Research Presentations

Invited Seminars: 2010 Iowa State University, Department of Genetics, Development & Cell Biology 2010 Indiana University School of Medicine, Department of Ophthalmology 2009 IUPUI, Department of Biology 2007 University of Wisconsin, WiCell Research Institute (recurring) 2004 University of Missouri, Department of Biology 2003 Harvard University, Schepens Eye Research Institute Symposia/Conference Presentations: Meyer JS, Capowski EE, Shearer RL, Wallace KA, Wright LS, Pinilla I, and Gamm DM (2009, October), Development of a model system for retinogenesis from a highly enriched population of human embryonic stem cell-derived retinal progenitors. Presented at the 2009 annual meeting of the Society for Neuroscience Meyer JS, Shearer RL, Capowski EE, Wright LS, McMillan EL, Zhang SC and Gamm DM (2008, November). Acquisition of a primitive retinal fate by human embryonic stem cells recapitulates normal development. Presented at the 2008 annual meeting of the Society for Neuroscience, Washington DC. Meyer JS, Gamm DM (2007-present). Use of human embryonic stem cells in neuroscience. Regularly invited speaker for the WiCell Foundation Introductory Course to Embryonic Stem Cells.

Jason S. Meyer Curriculum Vita November 2010 Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2004, September). Stem cell-mediated repair of the degenerating retina. Presented at the 13th Annual Growth Factor and Signal Transduction Symposium, Ames IA. Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2004, June). Stem cell-mediated repair of the degenerating retina. Presented at the 44th Annual Midwest Regional Developmental Biology Meeting, Kansas City, MO. Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2003, May), Neural-induced embryonic stem cells integrate into the retina and lead to a survival effect on photoreceptors in a mouse model of the neuronal ceroid lipofuscinoses. Presented at the 24th Midwest Neuroscience Meeting, Ames IA. Meyer JS, Katz ML, Maruniak JA, and Kirk MD (2003, April). Stem cell-mediated approaches to the neuronal ceroid lipofuscinoses. Presented at the 9th International Congress on the Neuronal Ceroid Lipofuscinoses, Chicago IL. Poster Presentations: Meyer JS, Wallace KA, Shearer RL, Capowski EE, Wright LS, and Gamm DM (2009, July), Modeling early retinal development with human embryonic and induced pluripotent stem cells, Presented at the 2009 meeting of the International Society for Stem Cell Research, Barcelona Spain. Meyer JS, Wallace KA, Shearer RL, Capowski EE, Wright LS, and Gamm DM (2009, April), Generation of Retinal Progenitor Cells and RPE From Human Induced Pluripotent Stem Cells, Presented at the 2009 meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale FL. Capowski EE, Meyer JS, Shearer RL, Wallace KA, Wright LS, and Gamm DM (2009, April), MITF Isoform Expression in Developing Human Retinal Cell Populations, Presented at the 2009 meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale FL. Meyer JS, Shearer RL, Capowski E, Wright LS, Lavaute TM, Pankratz M, Svendsen CN, Zhang SC, and Gamm DM (2008, April), The generation of early retinal cell types from human embryonic cells recapitulates a normal developmental timeline. Presented at the 2008 meeting of the Association for Research in Vision and Ophthalmology, Fort Lauderdale FL. Meyer JS, Wright LS, Shearer RL, Capowski E, Lavaute TM, Pankratz M, Svendsen CN, Zhang SC, and Gamm DM (2007, November). Acquisition of a primitive retinal fate by human embryonic stem cells mimics a normal developmental timeline. Presented at the 37th annual meeting of the Society for Neuroscience, San Diego CA.

Patents
Production, isolation, and use of multipotent retinal and forebrain progenitor neurospheres from human pluripotent stem cells (pending).

Jason S. Meyer Curriculum Vita November 2010

Funding
Pending Batten Disease Support and Research Association, Customized Stem Cell Approaches for the Neuronal Ceroid Lipofuscinoses, $19,500. American Health Assistance Foundation, National Glaucoma Research Foundation, Retinal Ganglion Cell Specification From Human Pluripotent Stem Cells, $100,000.

Teaching Experience
Teaching Positions University of Missouri-Columbia 2001-2002 Animal Physiology, Graduate Teaching Assistant 1999-2001 General Biology, Graduate Teaching Assistant Colgate University 1998 Animal Physiology, Undergraduate Teaching Assistant Commack Union Free School District 1999 7th Grade Physical Science, Teacher 1998-1999 Junior High Life and Physical Science, Teaching Assistant 1998-1999 Junior and Senior High Life Science Summer School, Teacher Formal University Training 2001-2002 Preparing Future Faculty Fellow, University of Missouri-Columbia 2001-2004 "Conversations in Life Science Teaching" Participant, University of Missouri-Columbia 2001 College Teaching Course, University of Missouri-Columbia 1994-1998 Teacher Certification Program, Colgate University (24 credit hrs) Student Mentorship 2009-present Mentored two undergraduate students on a research project examining the transcriptional regulation of retinal development from a primitive anterior neural population. 2005-2007 Provided mentorship to an undergraduate student on a research project examining the degenerative process in a mouse model of ALS. 2002-2003 Provided mentorship to an undergraduate student on a research project examining the ability of bone marrow-derived stem cells to acquire a neural fate.

Professional Memberships
Society for Neuroscience, Member International Society for Stem Cell Research, Associate Member Association for Research in Vision and Ophthalmology, Member

Stephen K. Randall, Ph.D.

2010

Curriculum vitae Randall, Stephen K. EDUCATION: Undergraduate: University of Connecticut, Storrs, CT, B.S. in Biology, 1976 Graduate: Indiana University, Bloomington, IN, Ph.D. in Biology, 1982 Post-doctoral: University of Maryland, College Park, MD, Botany Dept, 1982-1987 McGill University, Montreal, PQ, Canada, Biochem Dept, 1987-1990 ACADEMIC APPOINTMENTS: 2008-present 1996-present 1990-1995 1992-2001 1993-1996 1996-present Co-Director, IUPUI Signature Center for Membrane Biosciences Associate Professor, Department of Biology, IUPUI Assistant Professor, Department of Biology, IUPUI Faculty in Medical Biophysics Interdepartmental Program Adjunct Assistant Professor, Department of Biochemistry & Molecular Biology, IU School of Medicine, IUPUI Adjunct Associate Professor, Department of Biochemistry & Molecular Biology, IU School of Medicine, IUPUI

PROFESSIONAL AFFILIATIONS: Federation of American Societies for Experimental Biology American Society for Cell Biology American Society of Plant Physiologists Indiana Academy of Sciences Sigma Xi CURRENT TEACHING ASSIGNMENTS: Cellular Biochemistry (K484) , Independent 1 1 Research (K493) , Senior Thesis Research (K494) , Plant Biology Topics series, one 2 2 taught per year [ Topics in Plant Development (Biol 697), Topics in Plant Biochemistry, 2 2 Topics in Plant Genetics, Topics in Plant Growth and Development], Research: M.S. 1 thesis (Biol 698), Ph. D. (Biol 699) PAST TEACHING ASSIGNMENTS: Techniques in Biotechnology (Biol 548) , 3 Physiology of Membranes (F710)
1 2 1

indicates sole responsibility/year, indicates 50 % shared responsibility/year 3 indicates responsibility for three lecture/year (IUSM), not taught 2005, 2008-2010.

Stephen K. Randall, Ph.D.

2010

PROFESSIONAL SERVICE: a. University Co-director, IUPUI Signature Center for Membrane Biosciences (2009present) Radioactive Safety Committee (School Representative) Radioactive Drug Committee (School Representative) Radioactive Safety Council (School Representative) RSFG Standing Review Committee Budgetary affairs Committee (School Representative) At large IUPUI Faculty Council representative 2009-present) President School of Science Faculty Assembly (2007), Secretary School of Science Faculty Assembly (2006), Past-President School of Science Faculty Assembly (2008) General Studies Faculty Advisory Committee (School Representative) Council on Life-long Learning (School Representative) b. State and Regional Vice-Chair, Cell Biology section, Indiana Academy of Science (1992) Chair, Cell Biology section, Indiana Academy of Science (1993) Vice-Chair, Cell Biology section, Indiana Academy of Science (1999) Chair, Cell Biology section, Indiana Academy of Science, (2000) Mentor for LSAMP (Louis Stokes Alliances for Minority Participation) Program (2004) Mentor for McNair Scholarship Summer Research Program programs (20042006) Mentor for Minority Research Scholars Program b. National Chairman, Annual Meeting of the American Society for Plant Physiology, Cell Biology section (1992) Chairman, Annual Meeting of the American Society for Plant Physiology, Ion Transport section (1992) Chairman, Annual Meeting of the American Society for Plant Physiology, Enzymology and Metabolism III (1993) ad hoc Reviewer: Journals: Plant Physiology (Bethesda), Journal of Plant Physiology (Berlin), Plant Cell and Environment, Planta, Biochemistry, Cell Molecular Letters, Plant and Cell Physiology, Physiologia Plantarum. Plant Physiology and Biochemistry, Biologia Ad hoc reviewer: Grants: USDA, NSF, BARD Panel Member, NSF, Integrated Plant Biology (2002) Panel Member, NSF, Integrated Plant Biology (2004) Panel Member, NSF, Integrated Plant Biology (2005)

Stephen K. Randall, Ph.D.

2010

GRANTS AND FELLOWSHIPS AWARDED Internal Project Title Project Development Grant: Biogenesis of the higher plant vacuole Vacuolar-membrane biogenesis in higher plants Cellular and Biomolecular Spectroscopy, 1/1/94 (joint Biology and Chemistry proposal) I provided a support letter: The role of mitochondrial targetting sequences in the folding of precursor proteins. Identification of isoprenylated proteins in human breast cells. Elucidation of the Biochemical and physiological function of a vacuolar, calcium-binding proteins, VCaB42. Characterization of novel, isoprenylated heavy metal-binding proteins (co-PI, Dring Crowell) Elucidation of the Biochemical and physiological function of a vacuolar, calcium-binding proteins, VCaB42. Identifying targets for cold-stress in Arabidopsis using proteomics Funding source IUPUI NIH, Biomedical Research Support Grant (BRSG) Research Infrastructure Funds-IUPUI Duration 1991 19911992 1994 Total Award $1500 $3000

$251,094

The role of cold- and drought- stress responsive proteins in higher plants and the regulation of biochemical function by phosphorylation Pyrosequencing of cDNAs from cold-stress Fragaria vesca (strawberry), SK Randall P.I. Match with Graminor IUPUI Signature Center for Membrane Biosciences, Minto PI, Randall Co-PI , Wassall, Co-PI

American Cancer Society (Individual Allocation) Purdue Research Foundation Center for Earth and Environmental Sciences Purdue Research Foundation IUPUI Office for Professional Development (Grant-in Aidfor Research) IUPUI Office for Professional Development RSFG IUPUI International Development Funds (matched by Graminor, AC) IUPUI

19941995 19961997 1997

$8,000

$11,040 $4,000

19971998 20022003

$11,040 $3,000

20062007

$30,000

20072008

$7,500

20082011

$150,000

Stephen K. Randall, Ph.D.

2010

4 $34,000

Collaborative Development of Methods for IUPUI Center for Lipidomics and Proteomics in the membrane Center for Membrane Biosciences (CMB) Biology (Brenda Blacklock, Chemistry and Chemical Biology (CCB), PI; Robert Minto, CCB, and Stephen Randall, Biology, co-PIs)

2009-2010

External Project Title Molecular characterization of vacuolar membrane, calcium-binding proteins. S.K. Randall Research Opportunity Award (to support Dr. S. Speece) S.K. Randall Protein Prenylation in Plants (D. Crowell, S.K. Randall , Co-PIs) Research Experiences for Undergraduates (REU), Protein Prenylation in Plants (D. Crowell, S.K. Randall) Establishment of a Fluorescence Spectroscopy/cytometry laboratory (L.J. Jenski, PI, Co-PI's; W. Stillwell, S.K Randall, R.D. Allen) Protein isoprenylation in Plants (D.N. Crowell, , S.K. Randall) Prenylation of Oncogenic PTPCAAX Tyrosine Phosphatases (P.L. Crowell, P.I.; Co-P.I, D.N. Crowell, S.K. Randall Calcium-binding properties of cold- and dehydration-stress related proteins (S.K. Randall) Calcium-binding properties of cold- and dehydration-stress related proteins (S.K. Randall) Identification of molecular markers associated with winter survival in the cultivated strawberry, sub contract, (S.K. Randall) Pyrosequencing of cDNAs from cold-stress Fragaria vesca (strawberry), SK Randall P.I.

NSF, Cellular BioSciences NSF, Cellular BioSciences NSF, Cellular Biochemistry NSF, Cellular Biochemistry NSF

1992-1995 1993 1993-1996 1993

$202,707 $12,144 $240,000 $4,000

1994-1995

$36,780

NSF NIH USDA USDA Graminor, AC

1996-1999 1996-1999 1999-2002

$270,000 $469,519 $180,000

2002-2005, $125,000 no cost ext 2006 2007-2010 $116,961

Graminor, AC (matched with IUPUI

2007-2008

$10,000

Stephen K. Randall, Ph.D.

2010

Match with Graminor MRI: Acquisition of a High Resolution LC-MS/MS System, Minto, PI; Randall, Co-PI Sen CoPI, Blacklock CoPI Improving Winter Survival And Quality Of Norwegian Cultivated Strawberry (Alsheikh (P.I.), Randall, Rohloff, and Davik, Co-PIs) Identification of the stress signaling defect in commercial varieties of soybean through a comparative analysis with wild soybean.

International Development Funds) NSF-MRI

2008-2011

$479,590

Norwegian Research Council and Graminor Ac, United Soybean Board

2010-2014 $180,000

2010-2012

$150,000

RESEARCH PUBLICATIONS: 1IUPUI Graduate Student * indicates senior author 2IUPUI Undergraduate Student #indicates work performed IN RANK (since 1996) Authorship in the first and last position of a list of authors identifies the top two contributors to the work. These two authors often contribute equally, and thus both may be senior authors. Lesser contributions are indicated by authorship in the middle of the list of authors. PEER REVIEWED 1) Randall*, S.K., A.W. Ruesink. 1983 The orientation and integrity of plasma membrane vesicle obtained from carrot protoplasts. Plant Physiol. 73: 385-391. 2) Randall*, S.K., Y. Wang, H. Sze. 1985 Purification of the mitochondrial F1-ATPase from oat roots. Plant Physiol. 79: 957-962. 3) Randall*, S.K., H. Sze. 1986 Properties of the partially purified tonoplast H+-ATPase from oat roots. J. Biol. Chem. 261: 1364-1371. 4) Randall*, S.K., H. Sze. 1987 Probing the catalytic subunit of the tonoplast H+ATPase from oat roots. J. Biol. Chem. 262: 7135-7141. 5) Kaestner, K.H., S.K. Randall, H. Sze. 1988 Dicyclohexylcarbodiimide-binding proteolipid of the vacuolar proton ATPase from oat roots. J. Biol. Chem. 263: 1282-1287. 6) Lai, S., S.K. Randall, H. Sze. 1988 Peripheral and integral subunits in the tonoplast proton ATPase from oat roots. J. Biol. Chem. 263: 16731-16737. 7) Murali, N.S., Teramura, A.H., and S.K. Randall 1988 Response differences between two soybean cultivars with contrasting UV-B Radiation Sensitivities. Photochemistry and Photobiology 48 (5) pp. 653-657 8) Randall*, S.K., G.C. Shore. 1989 Import of a mutant mitochondrial precursor fails to respond to stimulation by a cytosolic factor. FEBS Letters 250: 561-564.

Stephen K. Randall, Ph.D.

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9) Randall*, S.K., H. Sze. 1989 Biosynthesis of the tonoplast proton ATPase. Plant Physiol. 89: 1292-1298. 10) Skerjanc, I., W.P. Sheffield, S.K. Randall, J. Silvias, G.C. Shore. 1990 Import of precursor proteins into mitochondria: Site of polypeptide unfolding. J. Biol. Chem. 265: 9444-9451. 11) Sheffield, W.P., G.C. Shore, S.K. Randall*. 1990 Mitochondrial precursor protein: Effects of 70 kDa heat shock protein on polypeptide folding, aggregation, and import competence. J. Biol. Chem. 265: 11069-11076. 12) Scherrer, L.C., K.A. Hutchison, E.R. Sanchez, S.K. Randall, and W.B. Pratt 1992 A heat shock complex isolated from rabbit reticulocyte lysate can reconstitute a functional glucocorticoid receptor-hsp90 complex. Biochemistry, 31 (32): 7325-7329 13) Randall*, S.K. 1992 Characterization of vacuolar, calcium-binding proteins. Plant Physiology, 100, 859-867 14) Randall*, S.K, M.S. Marshall, and D.N. Crowell, 1993 Protein isoprenylation in suspension-cultured tobacco cells. The Plant Cell, 5, 433-442 15) Biermann, B.J., T.A. Morehead2, S.E. Tate1, J.R. Price1, S.K. Randall, and D.N. Crowell. 1994 Novel isoprenylated proteins identified by an expression library screen. J. Biol Chem 269, (41) 25251-25254 16) Seals1, D.F., M.L. Parrish2, S.K. Randall*. 1994 A 42 kDa annexin-like protein is associated with plant vacuoles. Plant Physiology 106, 1403-1412 17) Morehead1, T.A., B.J. Biermann, D.N. Crowell, and S.K. Randall*, 1995 Changes in protein isoprenylation and growth in suspension-cultured tobacco cells. Plant Physiology 109, 277-284 #18) Cates1, C.A., Burke1, Y.D., Harvey1, K.A., Michael2, R.L. Stayrook1, K.R., Randall*, S.K., Crowell, P.L., and Crowell, D.N. 1996 Prenylation of oncogenic Human PTPCAAX protein tyrosine phosphatases. Cancer Lett. 110:49-55 #19) Biermann, B.J., Randall*, S.K., and Crowell, D.N. Identification and isoprenylation of plant GTP-binding proteins. 1996 Plant Molecular Biology 31: 1021-1028 #20) Seals1 D.F. and Randall* S.K. 1997 A vacuole-associated annexin protein, VCaB42, correlates with the expansion of tobacco cells. Plant Physiol 115, 753-761 #21) Frey1 R.K. and Randall* S.K. 1998 Initial steps in the assembly of the Vacuole-type + H -ATPase. Plant Physiol 118: 137-147 #22) Crowell, D.N., Sen S.E., and Randall, S.K. 1998 Prenylcysteine -carboxyl methyltransferase in suspension-cultured tobacco cells. Plant Physiology 118: 115-123 #23) Dykema, P.E., Sipes, P.R., Marie (formerly Zimeri), A., Biermann, B.J., Crowell, D.N., and Randall, S.K. 1999 A new class of proteins capable of binding transition metals. Plant Molec Biol 41, 139-150 #24) Lin, Y., Seals, D.F., Randall, S.K., and Yang, Z. 2001 Dynamic localization of Rop GTPases to the tonoplast during vacuole development. Plant Physiol 125, 241-251 #25) Heyen, B, Alsheikh, MK, Smith, E.A., Seals, D.F., and Randall, S.K. 2002 A vacuoleassociated calcium-binding protein, VCaB45 is regulated by phosphorylation. Plant Physiology 130, 675-687 #26) Mo, C, Valachovic, M, Randall, SK, Nickels, JT, and Bard, M 2002 Protein-protein interactions among C-4 demethylation enzymes involved in yeast sterol biosynthesis. Proc Natl Acad Sci USA 95 (15) 9739-9744

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#27) Werner, SR, Lee, PA, Schirtzinger, LM, DeCamp, MW, Crowell, DN, Randall, SK, and Crowell, PL 2003 Enhanced cell cycle progression and down regulation of p21cip1/Waf1 by PRL tyrosine phosphatases. Cancer Letters 202(2): 201-211. #28) Alsheikh, MK, Heyen, BJ, and Randall, SK 2003 Ion Binding properties of the dehydrin ERD14 are dependent upon phosphorylation. J. Biol Chem 278 (42) 40882-40889 (first published as JBC Papers in Press, August 13, 2003) #29) Alsheikh, MK, Svensson, JT, and Randall, SK 2005 Phosphorylation regulated ion-binding is a property shared by the acidic subclass dehydrins. Plant, Cell and Environment 28, 11141122 #30) Dumaual, CM, Sandusky, GE, Crowell, PL, and Randall, SK 2006 Cellular localization of PRL-1 and PRL-2 gene expression in normal adult human tissues. J. Histochemistry and Cytochemistry, (J Histochem Cytochem. 2006 Sep 6; [Epub ahead of print]) published in print: J. Histochem. Cytochem. 2006; 54: 1401 #31) Koehler, G, Weisel, TJ, and Randall SK, 2007 Transcript Expression Analysis Indicates Distinct Roles for Dehydrin Subclasses, Current Topics in Phytochemistry. 8, 73-83 #32) B. Teske, S. Taramino, M.S.A. Bhuiyan, N.S. Kumaraswami, S.K. Randall, R. Barbuch, J. Eckstein, G. Balliano, M. Bard, (2008) Genetic analyses involving interactions between the ergosterol biosynthetic enzymes, lanosterol synthase (Erg7p) and 3-ketoreductase (Erg27p), in the yeast Saccharomyces cerevisiae, BBA Molecular and Cell Biology of Lipids, Molecular and Cell Biology of Lipids Volume 1781, Issue 8, August 2008, Pages 359-366 doi: 10.1016/j.bbalip.2008.04.017 33) Rohloff, J., Kopka, J., Erban, A., Bones, A.M., Winge, P., Sparstad, Jrstad, T., Randall, S.K., Wilson, R., Davik, J., and Alsheikh, M. 2009 Metabolic, transcriptional, and proteomic profiling of cold response in Fragaria vesca. Acta Hort. (ISHS) 842:785-788 34) Koehler, G., Winge, P., Rohloff, J., Wilson, R., Alsheikh, M., Randall, S., 2010 Molecular cold responses for two strawberry cultivars: Comparison of proteomic and microarray analysis. Acta Hort. (ISHS) [submitted] 35) Rohloff, J., Koehler, G., Kopka, J., Erban, A., Wilson, R., Davik, J., Winge, P., Bones, A., Randall, S., and Alsheikh, M. An integrative omics approach reveals novel cold responses in fragaria [In review} BMP Plant Biology 36) Koehler, G., Wilson, R.C., Rohloff, J., Goodpaster, J.V., Sonsteby, A., Lai, Xianyin, Alsheikh, M., Randall, S.K. Proteomic study of low temperature responses in two strawberry varieties that differ in cold tolerance [in preparation].

BOOK CHAPTERS 1) Churchill, K.A., S.K. Randall, Y. Wang, H. Sze. 1984 Nitrate and DIDS inhibition of an anion sensitive, proton-pumping ATPase from oat roots. pp. 546-549 in J. Cram, K. Janacek, R. Rybova, K. Sigler (eds), Membrane Transport in Plants, Academia Praha, Czechoslovakia (proceedings of international symposia held in Prague, August 15-20, 1983.) 2) Sze, H., S.K. Randall, K.H. Kaestner, S. Lai. 1987 Vacuolar H+-ATPase from Oat Roots in C. Leaver, H Sze (eds) Plant Membranes: Structure, Function, Biogenesis. UCLA Symposium of molecular and cellular biology, Alan R Liss, Inc. New Series, Volume 63:195-207.

Stephen K. Randall, Ph.D.

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3) Randall*, S.K., H. Sze. 1987 Purification of the tonoplast anion-sensitive ATPase. Methods in Enzymology. 148, 123-131. #4) Crowell, D.N., B.J. Biermann, and S.K. Randall.* 1996 Identification of cDNA's encoding isoprenylated proteins. Molecular Biotechnology, Protocols Series. ,v.5, 253-258, Humana Press (USA). #5) Crowell, D.N., and Randall*, S.K. 1996 Protein farnesylation and phytohormone signal transduction. Trends in Plant Science 1, 407-408 #6) Randall*, S.K., and Crowell, D.N. 1999 Protein Isoprenylation in Plants, p 231-244. In Parish, E.J., and Nes, W.D., eds., Biochemistry and Function of Sterols. CRC Press, Inc., Boca Raton, FL Also published as: Randall* SK. Crowell DN. 1999 Protein isoprenylation in plants. [Review] Critical Reviews in Biochemistry & Molecular Biology. 34(5):325-38. TECHNICAL REPORTS #1) Patent entitled: Expression Library Screen by Prenylation of Expressed proteins, D.N. Crowell, B.J. Biermann, and S.K. Randall.* (Aug 3, 1999) Patent # 5,932,432 PLANT GENE REGISTER: Seals D.F. and Randall, S.K. 1999 Sequence analysis of a vacuole-associated annexin (accession number AF113545) from tobacco. (PGR 99-028) Plant Physiol 119. 1147. DNA SEQUENCES SUBMITTED TO GENBANK #Seals,D.F. and Randall,S.K., 1999 AF113545 Nicotiana tabacum vacuole-associated annexin VCaB42 (Anx3) mRNA, #Biermann,B.J., Randall,S.K. and Crowell,D.N.; 1996 U46929 Nicotiana tabacum GTP-binding protein NTGB3 mRNA U46928 Nicotiana tabacum GTP-binding protein NTGB2 mRNA U46927 Nicotiana tabacum GTP-binding protein NTGB1 mRNA U46926 Arabidopsis thaliana GTP-binding protein ATGB3 mRNA U46925 Arabidopsis thaliana GTP-binding protein ATGB2 mRNA U46924 Arabidopsis thaliana GTP-binding protein ATGB1 mRNA U64925 Nicotiana tabacum geranylgeranylated protein NTGP4 mRNA U64914 Nicotiana tabacum farnesylated protein NTFP2 mRNA U64913 Nicotiana tabacum farnesylated protein NTFP1 mRNA U64917 Glycine max farnesylated protein GMFP7 mRNA U64916 Glycine max farnesylated protein GMFP5 mRNA U64915 Glycine max farnesylated protein GMFP4 mRNA U64912 Arabidopsis thaliana farnesylated protein ATFP9 mRNA U64910 Arabidopsis thaliana farnesylated protein ATFP7 mRNA U64909 Arabidopsis thaliana farnesylated protein ATFP6 mRNA U64908 Arabidopsis thaliana farnesylated protein ATFP5 mRNA U64907 Arabidopsis thaliana farnesylated protein ATFP4 mRNA U64906 Arabidopsis thaliana farnesylated protein ATFP3 mRNA U64905 Arabidopsis thaliana farnesylated protein ATFP2 mRNA

Stephen K. Randall, Ph.D.

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U64904 Arabidopsis thaliana farnesylated protein ATFP1 mRNA U64926 Nicotiana tabacum geranylgeranylated protein NTGP5 mRNA U64924 Nicotiana tabacum geranylgeranylated protein NTGP3 mRNA U64923 Nicotiana tabacum geranylgeranylated protein NTGP2 mRNA U64922 Nicotiana tabacum geranylgeranylated protein NTGP1 mRNA U64921 Arabidopsis thaliana geranylgeranylated protein ATGP4 mRNA U64920 Arabidopsis thaliana geranylgeranylated protein ATGP3 mRNA U64919 Arabidopsis thaliana geranylgeranylated protein ATGP2 mRNA U64918 Arabidopsis thaliana geranylgeranylated protein ATGP1 mRNA U64911 Arabidopsis thaliana isoprenylated protein ATFP8 mRNA U13182 Glycine max clone GMFP6 isoprenylated protein mRNA U13181 Glycine max clone GMFP3 isoprenylated protein mRNA U13180 Glycine max clone GMFP2 isoprenylated protein mRNA U13179 Glycine max clone GMFP1 isoprenylated protein mRNA

PUBLISHED ABSTRACTS AND PRESENTATIONS 1Graduate student 2Undergraduate student 1) Randall*, S.K., A.W. Ruesink. 1981 Isolation and orientation of plasma membrane fragments obtained from carrot protoplasts. Botanical Society of America. Annual Meeting, Bloomington, Indiana. Misc Series Publication 160, p.51 2) Randall*, S.K., A.W. Ruesink. 1982 Proteins of the carrot plasma membrane. Annual meeting of the American Society of Plant Physiologists, Champaign, Urbana, IL. Plant Physiol. 69S: 94 3) Randall*, S.K., Y. Wang, H. Sze. 1984 Solubilization and purification of an anion sensitive ATPase from oat roots. Annual meeting of the American Society of Plant Physiologists, Davis, CA. Plant Physiol. 75S: 2 4) Randall*, S.K., H. Sze. 1984 Purification of the F1 component of the ATPase of oat root mitochondria. Annual meeting of the American Society of Plant Physiologists, Davis, CA. Plant Physiol. 75S: 22 5) Randall*, S.K., H. Sze. 1985 Identification of the structural components of the tonoplast ATPase from oat roots. Annual meeting of the American Society of Plant Physiologists, Providence, RI. Plant Physiol. 78S: 86 6) Randall*, S.K., S. Lai, H. Sze. 1986 Probing the functional subunits of the tonoplast ATPase (minisymposium I) Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA. Plant Physiol. 80S: 25 7) Randall*, S.K., K.H. Kaestner, H. Sze. 1987 Dicyclohexyl-carbodiimide binding to the 16 kDA proteolipid inhibits tonoplast ATPase activity. Annual meeting of the American Society of Plant Physiologists, St. Louis, MO. Plant Physiol. 83S: 112 8) Griffing, L.R., S.K. Randall, H. Sze, 1987 Ultrastructural localization of the nitratesensitive H+-ATPase. Annual meeting of the American Society of Plant Physiologists, St. Louis, MO. Plant Physiol. 83S, 45 10) Randall*, S.K., H. Sze. 1989 Biosynthesis of the tonoplast proton ATPase. Joint Meeting, American and Canadian Societies of Plant Physiologists, Toronto, Canada, Plant Physiol 89S: 157

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11) Randall*, S.K., G.S. Shore. 1989 Characterization of a cytosolic factor essential for the maintenance of import competence of a mitochondrial precursor protein. Annual Meeting of the American Society of Cell Biology, Houston, Texas. J. Cell Biol 109 (4) pt 2: 289a 12) Seals2, D.F., and S.K. Randall*. 1991 Identification and purification of a 40 kDa calcium-dependent, membrane binding protein associated with the vacuolar membrane in celery. Indiana Academy of Science, Evansville, IN. 107th Annual Meeting Program, p12 13) Randall*, S.K. 1991 Vacuole-specific, calcium-binding proteins. Indiana Academy of Science, Evansville, IN. 107th Annual Meeting Program, p14 14) Seals2, D.F., S. G. Babb2, and S.K. Randall*. 1991 Vacuole-specific calcium-binding proteins. Annual Meeting of the American Society of Plant Physiologists, Albuqueque, NM. Plant Physiol. 96S: 139 15) Frey1, R.K., and S.K. Randall*. 1992 In Vitro assembly of the vacuolar ATPase. Indiana Academy of Science, Ball State University, Muncie, IN. 108th Annual Meeting Program, p20 16) Seals1, D.F., and S.K. Randall*. 1992 Characterization of an annexin-like protein associated with celery vacuolar membranes. Indiana Academy of Science, Ball State University, Muncie, IN. 107th Annual Meeting Program, p20 17) Frey1, R. K., S.K. Randall*. 1992 In Vitro assembly of the V-type ATPase. Annual Meeting American Society of Plant Physiologists, Pittsburg, PA. Plant Physiol. 99S:40 18) Seals2, D. F., and S.K. Randall*. 1992 Purification and characterization of a calciumdependent, membrane-binding protein associated with vacuolar membranes. Annual Meeting American Society of Plant Physiologists, Pittsburg, PA. Plant Physiol. 99S: 25 19) Crowell, D.N., M.S., Marshall, and S.K. Randall*. 1993 Protein isoprenylation in cultured tobacco cells. ASBMB satellite meeting on Protein Prenylation. San Diego, CA FASEB Journal 7: 1042 20) Parrish2,M.L., and S.K. Randall*. 1993 The effect of a 42 kDa annexin-like protein on the calcium transport properties of the vacuole. Indiana Academy of Science, Cell Biology Section, Annual Meeting, W. Lafayette. 109th Annual Meeting Program, p22. 21) Seals1,D.F., and S.K. Randall*. 1993 Use of calcium green 2 as an indicator of free calcium concentrations: Application to determining the binding affinities of an annexinlike protein to vacuole membranes. Indiana Academy of Science, Cell Biology Section, Annual Meeting, W. Lafayette. 109th Annual Meeting Program, p22. 22) Tate1, S. E., D.N. Crowell, and S.K. Randall*. 1993 Approaches to identify cellular ligands of isoprenylated proteins. Indiana Academy of Science, Cell Biology Section, Annual Meeting, W. Lafayette. 109th Annual Meeting Program, p21. 23) Smith1, E.A., and S.K. Randall*. 1993 Purification of a 45 kDa calcium-binding protein associated with the celery vacuole. Indiana Academy of Science, Cell Biology Section, Annual Meeting, W. Lafayette. 109th Annual Meeting Program, p21. 24) Seals1, D.F., M.F. Parrish2, and S.K. Randall*. 1993 Characterization of an annexinlike protein associated with celery vacuole membranes. Annual meeting of the American Society of Plant Physiologists, Minneapolis, MN. Plant Physiol. 102S: 108. 25) Crowell, D.N., M.S. Marshall, and S.K. Randall*. 1993 In Vitro assay for farnesyl:protein transferase and geranylgeranyl:protein transferase Type I activity in

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cultured tobacco cells. Annual meeting of the American Society of Plant Physiologists, Minneapolis, MN. Plant Physiol. 102S:26 26) Randall*, S.K., L.J. Pajunar2, and D.N. Crowell. 1993 In Vivo labeling of tobacco cells with 14C-mevalonate reveals isoprenylated proteins. Annual meeting of the American Society of Plant Physiologists, Minneapolis, MN. Plant Physiol. 102S: 26 27) Seals1, D.F., K.S. Frasier2, S.E. Eichholtz2, and S.K. Randall*. 1994 Immunological characterization of a 42 kDa annexin-like protein in plants. Indiana Academy of Science, IU-South Bend, 110th Annual Meeting Program, p22. 28) Frey1, R.K. and S.K. Randall*. 1994 In vitro analysis of the assembly requirements of the vacuolar ATPase. Indiana Academy of Science, IU-South Bend, 110th Annual Meeting Program, 21 . 29) Price1, J.R., B.J. Biermann, T.A. Morehead1, S.E. Tate1, S.K. Randall, and D.N. Crowell. 1994 Identification of novel soybean cDNA's encoding isoprenylated proteins. Indiana Academy of Science, IU-South Bend, 110th Annual Meeting Program, p63 30) Tate1, S.E., B.J. Biermann, J.R. Price1, D.N. Crowell, and S.K. Randall*. 1994 Identification and purification of a novel isoprenylated protein encoded by soybean cDNA. Indiana Academy of Science, IU-South Bend, 110th Annual Meeting Program, p64. 31) Smith1, E.A. and S.K. Randall*. 1994 Purification and characterization of a 45 kDa calcium-binding protein (VCaB45) associated with the celery vacuole. Indiana Academy of Science, IU-South Bend, 110th Annual Meeting Program, p21. 32) Biermann, B.J., S.K. Randall, S.E. Tate1, J.R. Price1, and D.N. Crowell. 1994 Prenylated proteins encoded by soybean cDNA's. Annual meeting of the American Society of Plant Physiologists, July 30-August 3, 1994, Portland, Oregon. Plant Physiol. 105S: 153 33) Seals1, D.F., K.S. Frazer2, S.E. Eichholtz2, and S.K. Randall*. 1994 Immunological characterization of a novel annexin-like protein from celery. Annual meeting of the American Society of Plant Physiologists, 1994, Portland, Oregon. Plant Physiol. 105S, 103 34) Biermann, B.J., D.N. Crowell, T.A. Morehead2, and S.K. Randall*. 1994 A novel expression library screening method detects isoprenylated soybean proteins. Annual meeting of the American Society of Plant Physiologists, 1994, Portland, Oregon. Plant Physiol. 105S, 117 35) Biermann, B.J., T.A. Morehead1, S.E. Tate1, J.R. Price1, D.N. Crowell, and S.K. Randall*. 1994 Detection of putative signal transduction mediators with a novel expression library screening method. American Society for Cell Biology, National Meeting, San Francisco, CA, Molecular Biology of the Cell 5, 263a 36) Seals1, D.F., M.L. Parrish2, K.S. Frazer2, S.E. Eichholtz2, and S.K. Randall*. 1994 An annexin-like protein is associated with vacuole membranes. American Society for Cell Biology, Nat'l Meeting, San Francisco, CA, Molecular Biology of the Cell 5, 390a 37) Crowell, D.N., T.A. Morehead1, B.J. Biermann, and S.K. Randall*. 1995 Temporal expression of farnesyl:protein transferase activity in tobacco cultures. Annual meeting of the American Society of Plant Physiologists, Charlotte, NC Plant Physiol. 108S: 119 38) Crowell, D.N., B.J. Biermann, S.E. Tate1, J.R. Price1, and S.K. Randall*. 1995 Characterization of novel isoprenylated proteins from soybean. Annual meeting of the American Society of Plant Physiologists, Charlotte, NC Plant Physiol. 108S: 119

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39) Seals1, D.F., B.J. Biermann, and S.K. Randall*. 1995 Cloning of a putative vacuoleassociated annexin protein from Arabidopsis thaliana. Annual meeting of the American Society of Plant Physiologists, Charlotte, NC Plant Physiol. 108S: 21 40) Cates1, C., P. L. Crowell, S.K. Randall, and D.N. Crowell. 1995 Identification of human mammary carcinoma cDNA's encoding farnesylated proteins. Indiana Academy of Science, IUPUI, Indianapolis, 111h Annual Meeting Program, p78 41) Seals1, D.F., B.J. Biermann, and S.K. Randall*. 1995 Cloning the plant vacuolar annexin gene in arabidopsis. Indiana Academy of Science, IUPUI, Indianapolis, 111th Annual Meeting Program, p22. #42) Crowell, P.L., Cates1, C.A., Burke1, Y.D., Harvey1, K., Crowell, D.N., and Randall*, S.K. 1996 Prenylation of novel oncogenic human PTP-CAAX protein tyrosine phosphatases. Proc American Assoc for Cancer Research 37, 576 #43) Seals1, D.F., Anderson2, T.A., and Randall*, S.K. 1996 Expression of VCaB42 correlates with the presence of mature vacuoles in tobacco cells: possible role for VCaB42 in vacuole biogenesis. Annual Meeting for the American Society of Plant Physiologists. San Antonio, TX. #44) Randall*, S.K., Biermann, B.J., and Crowell, D.N. 1996 A novel class of isoprenylated metal-binding proteins. Annual Meeting for the American Society of Plant Physiologists. San Antonio, TX. #45) Morehead1, T.A., Crowell, D.N., and Randall*, S.K. 1996 Cytokinin restores starch accumulation to cultured tobacco cells treated with inhibitors of isoprenoid biosynthesis. Annual Meeting for the American Society of Plant Physiologists. San Antonio, TX. #46) Seals1, D.F., Randall*, S.K., and Vigil, E.L. 1996 Drought stress impacts expression of vacuolar proteins in cotton embryos. Annual Meeting for the American Society of Plant Physiologists. San Antonio, TX. #47) Crowell, P.L., Cates, C.A., Burke, Y.D., Harvey, K.A., Michael, R.L. Stayrook, K.R., Randall*, S.K., Crowell, P.L., and Crowell, D.N. 1996 Prenylation of oncogenic human PTPCAAX protein tyrosine phosphatases. Lilly/IU Cancer Research Symposium, Indianapolis, IN. 1 #48 Seals DF and Randall SK (1997) Investigating the relationship between cell growth and a vacuole-associated annexin. Washington, D.C. Molecular Biology of the Cell 8, 31a 2 2 #49) Randall SK, Zimeri A-M, Sipes PR, Dykema PE, and Crowell DN (1997) Isoprenylated metal-binding proteins. Washington, D.C. Molecular Biology of the Cell 8, 443a 1 #50) Seals DF, and Randall SK (1997) Investigating the relationship between cell growth and the expression of a vacuole-associated annexin. Annual Meeting of the American Society of Plant Physiologists, Vancover, CA Plant Physiol. Supplement 114 (3), 287 1 #51) Seals DF and Randall SK (1997) Investigating a vacuole-associated annexin protein relative to the expansion of suspension cultures tobacco cells. Indiana Academy of Science 1 2 #52) Schirtzinger LM, Ayoubi SA, Crowell DN, Randall SK, and Crowell PL (1997) Expression of PTPcaax mRNAs in tumor cells. Indiana Academy of Science

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#53) Morehead TA, McNew JA, Randall SK, and Crowell DN (1997) NTGP1, a putative vesicle-snare protein from yeast. School of Science Graduate Student Council Research Symposium, Nov 7, 1997 Indianapolis, IN 1 1 #54) Downes BP, Seals DF, Crowell DN, and Randall SK (1987) The molecular machinery of plant cell enlargement. School of Science Graduate Student Council Research Symposium, Nov 7, 1997 Indianapolis, IN 1 #55) Seals DF and Randall SK (1997) Vacuole formation in plants: Functional role of the annexin protein, VCaB42 Midwest Development Biology meeting. Indianapolis, IN 1 #56) Seals DF and Randall SK (1997) The expression of a vacuole-associated annexin protein, VCaB42, correlates with increased cell volume in tobacco suspension cultures. Plant Biochemistry, Physiology, and Molecular Biology Symposium. April 16-19, 1997 Columbia, MO 1 #57) Morehead TA, Randall SK, and Crowell DN (1997) Characterization of a novel isoprenylated V-SNARE protein from tobacco. Plant Biochemistry, Physiology, and Molecular Biology Symposium. April 16-19, 1997 Columbia, MO #58) Crowell, D.N., Sen, S E., and Randall, S.K. (1998) Prenylcysteine carboxyl methyl transferase in plants: role in membrane-association of isoprenylated proteins. Annual meeting of the American Society of Plant Physiologists , Madison, Wisconsin, Abstract # 239 #59) Dykema, P.E., Crowell, D.N., and Randall, S.K. (1998) Isoprenylation and protein/protein interactions. Annual meeting of the American Society of Plant Physiologists , Madison, Wisconsin, Abstract #244 1 1 #60) Lee , P.A., Schirtzinger , L.M. Crowell, D.N., Randall, S.K., and Crowell, P.L. (1998) Effects of PTPcaax expression on cell proliferation. Midwest Developmental Biology, Indianapolis, IN #61) Morehead T.A., Crowell, D.N., and Randall, S.K.(1999)Membrane localization and function of a putative isoprenylated vesicle-SNARE protein from Nicotiana tabacum. Annual meeting of the American Society of Plant Physiologists, Baltimore MD. #62) Dykema, P.E., Crowell, D.N., and Randall, S.K. .(1999) A subclass of Arabidopsis thaliana farnesylated, metal binding proteins are regulated during silique and seed development. Annual meeting of the American Society of Plant Physiologists, Baltimore MD. 1 1 #63) Foutch , JL Seals , D. F. Randall, S. K. (1999) Altered expression of VCaB42, a vacuolar calcium binding annexin, in transgenic tobacco cells. Annual meeting of the American Society of Plant Physiologists, Baltimore MD. Abstract #776 1 #64) Torvik , CF, and Randall, S. K. (1999) Characterization of VCaB45, an Arabidopsis Calcium Binding Dehydrin. . Annual meeting of the American Society of Plant Physiologists, Baltimore MD. Abstract #491 #65) Crowell, D.N., and Randall, S.K. (1999) regulation of protein function and localization by post-translational isoprenylation. AiBS St. Louis, MO 1 1 #66) Lee , P.A., Schirtzinger , L.M. Crowell, D.N., Randall, S.K., and Crowell, P.L. (2000) Effects of PTPcaax protein tyrosine expression on cell proliferation. Association for Cancer Research. 2 1 2 #67) Werner , SR, Lee , PA, Decamp , MW, Randall, SK, Crowell, DN, and Crowell, PL. (2000) Farnesylation of the oncogenic protein tyrosine phosphatase PTPCAAX2 is

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required for its nuclear localization. Proceedings of the American Association for Cancer Research 41, 219 (abstract # 1394) 2 #68) Asghar , MS, and Randall, SK (2001) Expression of the ATFP3 gene in E. coli. IUPUI Undergraduate Symposium, Spring #69) Randall, SK, Smith, EA, and Heyen, BJ (2001) The calcium binding properties of a dehydrin protein are regulated by phosphorylation. Annual meeting of the American Society of Plant Physiologists, Providence R.I. Abstract #386 2 1 #70) Werner , SR, Moore , SA, Crowell, DN, Randall, SK, and Crowell, PM 2002 Overexpression of the PRL-2 protein tyrosine phosphatase gene in ovarian, uterine, breast, rectal, and stomach cancers: Association with down regulation of the cyclin dependent kinase inhibitor p21. Proceedings of the American Association for Cancer Research 43, 472. #71) Alsheikh, MK, and Randall, SK 2002 Calcium-binding activity of the dehydrin, ERD14, is regulated by phosphorylation. Mid-West section American Society of Plant Biologists. Miami University, Oxford, Ohio March 15-16 #72) Alsheikh, MK, Heyen, BJ, and Randall, SK (2002) Calcium-binding activity of the dehydrin, ERD14, is regulated by phosphorylation. Annual meeting of the American Society of Plant Biologists. Abstract #818, Denver, CO. 1 #73) Livingston , MR, Crowell, DN, Crowell, PL, and Randall, SK 2002 Identification of PTPCaaX2 protein interactors by T7 Phage display. October 11 Indiana Academy of Science V118: p 39, Butler University, Indianapolis, IN. #74) Alsheikh, MK and Randall, SK 2002 Calcium-binding activity of the dehydrin, ERD14, is regulated by phosphorylation. October 11 Indiana Academy of Science V118: p 37, Butler University, Indianapolis, IN. #75) Christman2 DL, and Randall SK 2002 RNA distribution and protein localization of ATFP3. October 11 Indiana Academy of Science V118: p 37, Butler University, Indianapolis, IN #76) Mo, C, Randall, S, and Bard, M 2002 Can we identify proteins in the ergosterol enzymatic complex? INGEN Proteomics Symposium, November 15, 2002, Indianapolis #77) Alsheikh, M and Randall, SK 2003 Identification of phosphorylation sites associated with the activation of calcium-binding of the dehydrin, ERD14. Purdue Proteomics Symposium, Feb 21, 2003 W. Lafayette, IN 1 1 2 #78) Werner , SR, Zambrano , CM, Sandusky, GE, Cummings , OW, Randall, SK, Crowell, DN, and Crowell, DN 2003 American Association for Cancer Research April 59, 2003 Toronto, CA #79) Alsheikh, MK, Svensson, J, and Randall, SK 2003 Ion binding of dehydrins is dependent on phosphorylation. Annual Meeting of the American Society of Plant Biologists, Abstract #899 , Honolulu, Hawaii #80) Alsheikh, MK and Randall, SK 2003 Phosphorylation regulated ion-binding is a property shared by several acidic subclass dehydrins. October 17, Indiana Academy of Science V119: p36 Anderson University, IN 1 #81) Koehler , G, Alsheikh, MK, and Randall, SK 2003 Subcellular location studies of dehydrins. October 17, Indiana Academy of Science V119: p38 Anderson University, IN 1 #82) Rusnak , KB, and Randall, SK 2003 Investigating the structure and function of ERD14. October 17, Indiana Academy of Science V119: p86 Anderson University, IN

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#83) Alsheikh, MK, Heyen, BJ, and Randall, SK 2003 Biochemical characterization of the stress-induced dehydrins. Mid-West Section Meeting of American Society of Plant Biology. March 20-21, Ames, Iowa #84) Randall, SK, Svensson, J, and Alsheikh, MK 2004 Phosphorylation dependent ion binding activity of acidic dehydrins. Plant Responses to Abiotic Stress, Keystone Symposium Santa Fe, NM Feb 19-23, 2004 1 #85) Koehler , G, Alsheikh, MK, and Randall, SK 2004 Localization of Dehydrins. April 14, 2004, Sigma Xi Competition, Indianapolis, IN 1 #86) Klanrit P, Webb MA, Pruitt RE, Lolle SJ, and Randall SK 2004 Hip (Hsp70interacting protein) is a calcium-binding protein. 8th ICCBMT (International Conference on the Chemistry and Biology of Mineralized Tissues), October 17-22, 2004, Banff Centre, Alberta, Canada 1 2 2 #87) Gage Koehler , Mohammed Abdul Moid , Don E. Fish Muath K Alsheikh, and Stephen K. Randall 2005 Subcellular localization and function of Arabidopsis dehydrins. Annual Meeting of the American Society of Plant Biologists, Abstract # Seattle, WA 1 #88) Gage Koehler and Stephen Randall 2005 Location and activation of dehydrins in Arabidopsis thaliana. Indiana Academy of Science V121: p48 St. Mary of the Woods, IN 2 1 2 #89) Mohammed Abdul Moid , Gage Koehler , Don E. Fish , and Stephen K. Randall 2005 Site-directed mutagenesis of ERD14. Indiana Academy of Science V121: p48 St. Mary of the Woods, IN 2 1 #90) Abdul Moid M, Koehler G, Randall SK 2006 Phosphorylation of the dehydrin , ERD14. MidWest Section, American society of Plant Biologists, Chicago, Ill March 24, 2006 2 #91) Mohammed Abdul Moid and Stephen K. Randall 2006 Site-directed mutagenesis of ERD14. 2006 IUPUI Undergraduate Research Symposium, Indianapolis, IN 1 #92) Yamasaki Y, Randall, SK. 2006 Characterization of dehydrins in Soybean. T/P ## MidWest Section, American Society of Plant Biologists, Chicago, Ill March 24, 2006 2 #93 Moid , Abdul, and Randall Stephen K 2006 Site-directed mutagenesis of possible phosphorylation sites in the dehydrin, ERD14 P09022, National Meeting of ASPB Aug 5-Aug 9, 2006, Boston, MA 1 #94 Koehler , Gage, and Randall, Stephen 2006 Characterization of the kinase responsible for phosphorylating the dehydrin Erd14. P09024 National Meeting of ASPB Aug 5-Aug 9, 2006, Boston, MA #95 ) M. Alsheikh, R. Wilson, J. Davik, A. Snsteby, and S. Randall 2006 Biotechnology plant breeding: Integration of omics technologies in the development of superior varieties. 5th Norwegian Arabidopsis meeting, Trondheim University, Norway 1 #96) Yamasaki Y, and S.K. Randall 2007 The analysis of vegetative dehydrin-like proteins in soybean. Abstract # 1833 National Meeting of ASPB, Chicago, Aug, 2007 1 #97) Koehler , G, and Randall SK 2007 Evaluating dehydrin expression in Arabidopsis thaliana. Abstract # 1864 National Meeting of ASPB, Chicago, Aug, 2007 1 #98) Koehler , G, Alsheikh , M, Wilson, R, Rohloff, J , Randall, S 2009 Comparative Analysis of Protein and Transcripts Associated with Cold Response in Cultivated

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Strawberry, Annual meeting American Society for Plant Biology, Hawaii, poster 08110 Abstract p. 157 1 #99) Yamasaki , Y, and Randall S 2009 Regulation of dehydrin expression in soybean 2009 Annual meeting American Society for Plant Biology, Hawaii, poster P08108, Abstract p. 157 1 #100) Yamasaki Y, and S.K. Randall 2009 Transcription factors and acidic dehydrin expression in Glycine max. Indiana Academy of Science 125th Annual Meeting October 23, 2009, Program p. 114 1 #101) Koehler , G, Alsheikh, M., Wilson, R., Rohloff, J, Goodpaster, J.V., and Randall SK 2009 Comparative analysis of proteins in two strawberry varieties that differ in freezing tolerance. Indiana Academy of Science 125th Annual Meeting October 23, 2009, program p. 110 1 #102) Alsheikh, M. Koehler , G., Rohloff, J., Wilson, R., Form, B., Sonsteby, A, Davik, J., and Randall, S. 2010 Identification of molecular markers associated with winter survival in the cultivated strawberry by integration of omics technologies. 28th International Horticultural Congress, Lisbon, Spain #103) Rohloff, J., Winge, P., Davik, J., Brurberg, M.B., Mockaitis, K., Shulaev, V., Randall, S.K., Bones, A.M., Alsheikh, M. 2010 Development of a customized Fragaria microarray chip and its application to abiotic and biotic stress responses in Fragaria sp. 28th International Horticultural Congress, Lisbon, Spain # 104) Rohloff, J., Randall, S.K., Bones, A.M., Alsheikh, M. 2010 Short- and long-term metabolic regulation in five strawberry cultivars under cold acclimation. 28th International Horticultural Congress, Lisbon, Spain

As of 2007, 28 undergraduates have appeared on abstracts or delivered talks.

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PRESENTATIONS: 1) Randall*, S.K., K.H. Kaestner, S.P. Lai, H. Sze. 1986 Probing the functional subunits of the tonoplast H+-ATPase from oat roots. 7th International Workshop on Plant Membrane Biology, Sidney, Australia. 2) Randall*, S.K., G.S. Shore. 1988 Characterization of cytosolic factors that stimulate import of mitochondrial precursor, pOCT. 4th Internat'l Congress of Cell biology, Montreal, August 14-19, 1988. 3) Randall*, S.K., K.H. Kaestner, S. Lai, H. Sze. 1988. Vacuolar H+-translocating ATPase of a higher plant. 14th Internat'l Congress of Biochemistry, Prague, Czechoslovakia, July 10-15, 1988. 4) Lai, S.P., S. K. Randall, and H. Sze. 1992 In vitro biosynthesis and membrane insertion of the 16 kDa proteolipid subunit from the vacuolar H+-translocating ATPase of oats. American Society for Cell Biology, Summer Research Conference, Airlie, Virginia. 5) Randall*, S.K., R.K. Frey1, and S.G. Babb2. 1992 Assembly of the vacuolar-type ATPase. 9th Internat'l Plant Membrane Workshop. Monterey, CA. 6) Frazer2, K. S., D.F. Seals1, and S.K. Randall*. 1995 Association of a calcium-binding protein with the higher plant vacuole. Poster presented at the E.C. Moore Symposium. 7) Randall, S.K., Frey, R.K., Foutch, J.L., and Seals, D.F. (1999) Studies on vacuole biogenesis and function. Annual meeting of the American Society of Plant Physiologists, Baltimore MD. Abstract # 44003 invited Mini-Symposium. THESIS COMMITTEES Undergraduate

(Senior thesis is Biology's capstone experience): >15 completed 5 at present Graduate level (M.S. nonthesis): 12 (4 at present) Graduate level (M.S. thesis): 12 completed (chaired 6) 2 chaired at present Graduate level (Ph.D.): 1 completed (chaired) 2 chaired at present

Mentor: School of Science Minority Research Scholarship Program, McNair Scholars Program, and LSAMP INVITED SEMINARS: 1) 2) 3) 4) 5) 6) Molecular/Cellular Biophysics Group, IUPUI (1990) Department of Plant Biology, University of Illinois (1991) Central Indiana Biology Teachers Focus Group (1992) Department of Biochemistry & Molecular Biology, IU School of Medicine (1992) Department of Botany and Plant Pathology, Purdue University (1993) The Renal Research Conference, IU School of Medicine (1993)

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7) Biology Department,Wabash College (1994) 8) Botany Department, University of Maryland (1994) 9) Biology Department, Indiana University-Purdue University at Fort Wayne (1995) 10) Medical Biophysics Seminar Series, IU Medical School and IUPUI School of Science (1995) 11) Biology Department, Purdue University (W. Lafayette), Philosophy of Biological Instruction Program (1996) 12) Annual meeting of the American Society of Plant Physiologists, Baltimore MD. Studies on vacuole biogenesis and function. Invited Mini-Symposium. (1999) 13) The Phosphorylation Workshop, Jackson Hole, Colorado Calcium-binding activity of dehydrins is regulated by dehydrins.(2002) 14) The Genetics/Physiological and Molecular Plant Biology (PMPB) group, University of Illinois, Champaign-Urbana, Title: Cold Stress, Dehydrins, and Phosphorylation (2003) 15) The Phosphorylation Workshop, Snowbird, Utah, October 28-31, 2004 In vitro interaction methods: Phage display and biotin labeling 16) LSAMP Summer Research Training Program at IUPUI, Biology Research Projects June 22, 2004. 17) Butler University 4-20-07, Impact of the Environment on Plant Productivity and Survival 18) Graminor, AC., Ilseng, Norway, 3-15-07, Understanding how plants respond to cold and drought, and mineral stress 19) The Norwegian University of Life Sciences, Aas, Norway, 3-17-07, Understanding how plants respond to cold and drought stress 20) LSAMP, 6-07, program presentation. 21) Food For Thought: Local Food, Global Impact, sponsored by IUPUI Office of Alumni Relations, IUPUI, March 6, 2010 Genetic Engineering of Food - w/John Watson 22) Biology Department, Exploring Environmental Stress Responses in Higher Plants departmental seminar, April 9, 2010 23) LSAMP research presentation, IUPUI, June 1, 2010, Soybeans and Biofuels Project 24) Atopic Disease Research Forum, August 11, 2010 "Creating hypoallergenic strawberries" Herman B Wells Center for Pediatric Research Indiana University School of Medicine

_________________________________________________________ (Date) Stephen K. Randall

RANDALL J. ROPER, PH.D.

ASSISTANT PROFESSOR DEPARTMENT OF BIOLOGY INDIANA UNIVERSITY-PURDUE UNIVERSITY INDIANAPOLIS (IUPUI) 723 WEST MICHIGAN STREET, SL 306, INDIANAPOLIS, IN 46202 PHONE: (317) 274-8131 FAX: (317) 274-2846 EMAIL: RJROPER@IUPUI.EDU

EXPERIENCE
Assistant Professor, Department of Biology, IUPUI 2006Investigator, Indiana Univ. Center for Regenerative Biology and Medicine 2006 Principal Investigator of basic science laboratory investigating genetic, cellular and molecular mechanisms leading to developmental phenotypes associated with Down syndrome (DS) Manage clinical investigation of neonatal phenotypes associated with DS to improve diagnosis and care of infants with DS Lead teams of ~10 individuals in basic science and clinical research environments Consultant, Marathon Solutions Corporation, Carmel, IN 2008-2010 Determined best practices and design for expanding research opportunities in health care organizations Postdoctoral Fellow, Johns Hopkins University School of Medicine 2001-2006 Department of Physiology, Laboratory of Dr. Roger Reeves Performed nationally acclaimed research in Department of Physiology and McKusickNathans Institute of Genetic Medicine on genetic, developmental and molecular biomarkers and etiology of traits associated with Trisomy 21 or DS Analyzed therapy to correct structural and neuronal deficiencies in DS mice Engineered new mouse models of DS using embryonic stem cells and cre/lox recombination Research Assistant, University of Illinois at Urbana-Champaign 1996-2001 Department of Veterinary Pathobiology, Laboratory of Dr. Cory Teuscher Localized quantitative trait loci (QTL) controlling susceptibility in mouse models of ovarian and testicular autoimmunity, multiple sclerosis and Lyme arthritis Collaborated with computer scientists and statisticians to apply permutation theory in QTL analysis using parallel processing on a supercomputer Research Assistant, Brigham Young University 1994-1996 Department of Microbiology, Laboratory of Drs. Richard Sagers and Bill Pitt Tested synergistic effect of ultrasound on antibiotics against P. aeruginosa and E. coli

EDUCATION AND TRAINING

Johns Hopkins University School of Medicine, Baltimore, MD 2001-2006 Postdoctoral Fellow with Dr. Roger H. Reeves Department of Physiology and McKusick-Nathans Institute of Genetic Medicine Research: Genetic, cellular, and molecular etiology of Down syndrome phenotypes University of Illinois at Urbana-Champaign, Urbana, IL 2001 Ph.D. Immunology and Genetics, Department of Veterinary Pathobiology Dissertation: Genetic control of experiment. induced reproductive autoimmune phenotypes Dissertation advisor: Dr. Lawrence B. Schook, Research advisor: Dr. Cory Teuscher Brigham Young University, Provo, UT 1995 B.S. Microbiology, Emphasis: Molecular Biology International Spring Stereology Workshop, Washington, DC 2002 The Jackson Laboratory, Bar Harbor, ME 2001 42nd Annual Short Course in Medical and Experimental Mammalian Genetics North Carolina State University, Raleigh, NC 1998 Summer Institute in Statistical Genetics

RANDALL J. ROPER, PH.D.

GRANTS, FELLOWSHIPS, AND OUTSTANDING ACHIEVEMENTS

National Institutes of Health (NIH) 2010-2013 NIDCR, Genetic mechanism of neural crest deficits in Down syndrome mice DE021034 $379,659 Blue River Community Foundation /Riley Childrens Foundation 2008-2010 Down Syndrome Research $4000 Jerome LeJuene Foundation, Paris, France 2011-2013 Mechanisms of craniofacial and appendicular bone development disrupted by trisomy in Down syndrome Pending R.J. Roper, P.I. Purdue Research Foundation 2008 Summer Faculty and International Travel Grants $9000 IUPUI Office of Research and Sponsored Programs 2008 Research Support Funds Grant $35,000 IUPUI Honors Program Research Fellow $6000 2007-2010 NIH Ruth L. Kirschstein National Research Service Award 2003-2006 NICHD, Mouse Models of Down Syndrome, HD043614 $138,000 IUPUI Trustees Teaching Award 2010 Positive impact on learning through the direct teaching of students Mentor of the Year Award 2010 Louis Stokes Alliances for Minority Participation (LSAMP), IUPUI Outstanding Research Poster, 18th International Mouse Genome Conference 2004 Mariangela and Diego Segre Graduate Fellowship 2001 Outstanding Poster Presentation, CMBTG Symposium, University of Illinois 2000 NIH Cell and Molecular Biology Training Grant 1997-2000 University of Illinois, Trainee, GM07283 University Fellowship for Academic Merit and Research Potential 1998-2001 University of Illinois Undergraduate Student Research Award 1994 Office of Research and Creative Work, Brigham Young University Trustees Scholar, Brigham Young University, Full tuition academic scholarship 1992-1995

TEACHING EXPERIENCE
Assistant Professor, Implantable Materials and Biological Response 2006Undergraduate Course, Department of Biomedical Engineering, IUPUI Assistant Professor, Molecular Genetics and Development Graduate Course 2008Department of Biology, IUPUI Major Professor for Graduate Students, IUPUI 2007Ph.D.: Joshua Blazek M.S.: Justin VanHorn, Jared Allen, Nichole Duvall, Cherie (Nicci) Billingsley, Samantha Deitz, Jeffrey Solzak Primary Research Advisor for Undergraduate Students, IUPUI 2006Students completed 2 semesters Independent Research and 1 semester Thesis writing: Heather Rumsey, Areef Kassam, Junita Singh, Sashana Gordon Jackson, Danny Carney, Emily Merkel Thomas, Samantha Deitz , Abby Newbauer, Andrew Darrah, Brady Harman Teaching Assistant, Organ Systems and Developmental Biology Courses 2004-2006 Johns Hopkins University School of Medicine Lecturer, Concepts and Topics in Immunology, University of Illinois 1999-2001 Lecturer and Teaching Assistant, Immunobiology Course & Microbiology Labs 1993-1995 Brigham Young University Spanish Instructor and Interpreter 1992-1997 Brigham Young University, Bromley Farms (Am. Fork UT), WORDLINK, Inc. (Champaign IL)

RANDALL J. ROPER, PH.D.

UNIVERSITY SERVICE

Chair, Graduate Recruitment Committee, Department of Biology, IUPUI 2010Dept. Chair Search Committee, Dept. of Biology, School of Science, IUPUI 2009-2010 Curriculum Committee, Department of Biology, IUPUI 2009-2010 Project SEED Mentor, IUPUI 2009 Institutional Biosafety Committee, Butler University, Indianapolis, IN 2008GK-12 Training Program Mentor, National Science Foundation (NSF) IUPUI 2008Chair, Cell and Developmental Biology Ph.D. Qual. Exam Committee, IUPUI 2008Master Planning Committee, School of Science, IUPUI 2008 Faculty Search Committees, Dept. of Biology and Regen. Biology and Medicine 2008 Department Graduate Committee Member, Department of Biology, IUPUI 2006-2009 Louis Stokes Alliances for Minority Participation (LSAMP) Mentor, IUPUI 2007Diversity Scholars Research Program (DSRP) Mentor, IUPUI 2006-2008 Program Committee, Biotechnology and Bioinformatics Symposium, (BIOT) 2007 Postdoctoral Representative, Medical School Council and University Health Services Advisory Committee, Johns Hopkins University School of Medicine 2002-2005 Vice President, Policy, Johns Hopkins University Postdoctoral Association 2002-2004 Co-Chair, Department Research Seminars, Department of Physiology, Johns Hopkins University School of Medicine 2002-2003 Student Member, NIH Cell and Molecular Biology Training Grant Executive Committee, University of Illinois 1999-2001 Committee Member, Department Seminar Series, Dept of Veterinary Pathobiology, University of Illinois 1999-2000 Co-Chair, NIH Cell and Molecular Biology/Molecular Biophysics Training Grant Annual Symposium, University of Illinois 1997-1999

PROFESSIONAL ORGANIZATIONS AND OTHER ACTIVITIES

Professional Organizations American Association for the Advancement of Science American Society of Human Genetics International Mammalian Genome Society Society for Developmental Biology Ad hoc Reviewer Manuscripts: American Journal of Medical Genetics: Part A, Brain Pathology, Genesis, Genome Biology, International Journal of Experimental Pathology, Journal of Pediatric Neurology, Disease Markers Grant Proposals: Research Grants Council (RGC) (Hong Kong), Medical Research Council (MRC)(London), Undergraduate Research Opportunities Program, Center for Research and Learning (IUPUI), Pew Scholars Program in Biomedical Sciences (IUPUI and Indiana Universities) Media Relations Interview with Dick Wolfsie, Mice give insight into Down syndrome, Broadcast on WISH TV, (Indianapolis, IN) January 15, 2009 (www.wishtv.com/dpp/on_tv/wolfsie/Mice_give_insight_to_Down_syndrome) YouTube recruitment for the Department of Biology, Discover Biology @ IUPUI School of Science, (www.youtube.com/user/IUPUIScience#p/u/0/ZXhP9EFGfzs) IUPUI Magazine, Experiment: Life in a lab offers many roles (Mentoring Samantha Deitz) Fall 2010 Session Moderator, 22nd Annual Butler University Undergraduate Research 2010 Conference, (Indianapolis, IN) Team Leader, Buddy Walk, Down Syndrome Indiana 2007Volunteer Leader and Counselor, Boy Scouts of America 1997-

RANDALL J. ROPER, PH.D.

PUBLICATIONS AND PRESENTATIONS

JOURNAL ARTICLES: Blazek,J.D., A. Gaddy, R. Meyera, R.J. Roper*, and J. Li. 2010. Disruption of bone homeostasis by trisomy in Ts65Dn Down syndrome mice. Bone. 2010 Sep 24. [Epub ahead of print] (*Roper is corresponding author). Oviatt D., M. Clement , Q. Snell, K. Sundberg, C.W. Lai, J. Allen, and R.J. Roper. 2010. Inferring gene regulatory networks from asynchronous microarray data with AIRnet. BMC Genomics 11(Suppl 2):S6. Deitz,S.L. and R.J. Roper. 2010. Trisomic and allelic differences influence phenotypic variability during development of Down syndrome mice. Submitted to Genetics. Under revision. Billingsley, C.N., J.A. Allen, J.D. Blazek, D.D. Baumann, A. Newbauer, A. Darrah, B.C. Long, B. Young, M. Clement, R.W. Doerge, and R.J. Roper. 2010. Altered mandibular development in Down syndrome mouse model linked to modified expression of non-trisomic genes. Submitted to Human Molecular Genetics. Blazek, J.D., C.N. Billingsley, A. Newbauer, and R.J. Roper. 2010. Embryonic and not maternal trisomy causes developmental attenuation in the Ts65Dn mouse model for Down syndrome. Dev Dyn. Jun;239(6):1645-53. (Highlighted in Dev Dyn. Dissecting Down syndrome models Oct 2010) Lorenzi, H., N. Duvall, S. M. Cherry, R. H. Reeves, and R. J. Roper. 2010. PCR prescreen for genotyping the Ts65Dn mouse model of Down syndrome. Biotechniques. 48(1):35-8. Allen J.R., J. E. Buckingham, R. J. Roper, and K. A. Marrs. 2010. How Middle School Students Come Face to Face with Down Syndrome Research. Science Scope. 33:20-25. Clement K., N. Gustafson, A. Berbert, H. Carroll, C. Merris, A. Olsen, M. Clement, Q. Snell, J. Allen, and R.J. Roper. 2010. PathGen: A transitive gene pathway generator. Bioinformatics. Feb 1;26(3):423-5. Epub 2009 Dec 4. Roper, R.J.*, J.F. VanHorn, C. Cain, and R.H. Reeves*. 2009. A neural crest deficit in Down syndrome mice is associated with deficient mitotic response to Sonic hedgehog. Mech Dev. 126:212-9. Epub 2008 Nov 21. (*Roper and Reeves are corresponding authors). Samy ET, K.M. Wheeler, R.J. Roper, C. Teuscher, and K.S. Tung. 2008. Cutting edge: Autoimmune disease in day 3 thymectomized mice is actively controlled by endogenous disease-specific regulatory T cells. J Immunol. 180:4366-70. Moore C.S. and R. J. Roper, 2007. The power of comparative and developmental studies for mouse models of Down syndrome. Mamm Genome. 18:431-43. Epub 2007 Jul 26. (Invited review for Mammalian Genome Special Issue Comparative Analysis of Systems: From Genomes to Physiology). VanHorn, J.F., and R.J. Roper, 2007. In vitro conformation of Down syndrome modifiers from pathway analyses. Proceedings of the 4th Biotechnology and Bioinformatics Symposium, pp. 7-11. Rungta, N., H. Carroll, E.G. Mercer, R.J. Roper, M. Clement, and Q. Snell. 2007. Analyzing gene relationships for Down syndrome with labeled transition graphs. FMCAD '07: Proceedings of the Formal Methods in Computer Aided Design, IEEE Computer Society, Washington DC, pp 216-219. (This is a peer-reviewed full-length publication).

RANDALL J. ROPER, PH.D.

PUBLICATIONS AND PRESENTATIONS (CONTINUED)

Journal Articles (Continued): Olson, L.E., R. J. Roper, C. L. Sengstaken, E. A. Peterson, V. Aquino, Z. Galdzicki, R. Siarey, M. Pletnikov, T. H. Moran and R. H. Reeves. 2007. Trisomy for the Down syndrome critical region is necessary but not sufficient for brain phenotypes of trisomic mice. Hum Mol Gen. 16:774-82. Epub 2007 Mar 5. Roper, R.J. and R.H. Reeves. 2006. Understanding the basis for Down syndrome phenotypes. PLoS Genet. Mar;2(3):e50. Roper, R.J.*, L.L. Baxter*, N.G. Saran, D.K. Klinedinst, P.A. Beachy and R.H. Reeves. 2006. Defective cerebellar response to mitogenic Hedgehog signaling in Down syndrome mice. Proc Natl Acad Sci U S A. 103:1452-6. Epub 2006 Jan 23. (*Roper and Baxter are co-first authors). Roper, R.J., H.K. St. John, J. Philip, A. Lawler, and R.H. Reeves. 2006. Perinatal loss of Ts65Dn Down syndrome mice. Genetics. 172:437-43. Epub 2005 Sep 19. Olson, L.E., R.J. Roper, L.L. Baxter, E.J. Carlson, C.J. Epstein, and R.H. Reeves. 2004. Down syndrome mouse models Ts65Dn, Ts1Cje, and Ms1Cje/Ts65Dn exhibit variable severity of cerebellar phenotypes. Dev Dyn. 230:581-9. Roper, R.J., R.D. McAllister, J. E. Biggins, S.D. Michael, S.H. Min, K.S. Tung, S.B. Call, J Gao, and C. Teuscher. 2003. Aod1 controlling day three thymectomy-induced autoimmune ovarian dysgenesis in mice encompasses two linked quantitative trait loci with opposing allelic effects on disease susceptibility. J Immunol. 170:5886-91. Butterfield, R.J.*, R.J. Roper*, D.M. Rhein, R.W. Melvold, L. Haynes, R.Z. Ma, R.W. Doerge, and C. Teuscher. 2003. Sex specific quantitative trait loci govern susceptibility to Theilers murine encephalomyelitis virus-induced demyelination (TMEVD). Genetics. 163:1041-6. (*Butterfield and Roper are co-first authors). Roper, R.J., R.Z. Ma, J.E. Biggins, R.J. Butterfield, S.D. Michael, K.S.K. Tung, R.W. Doerge, and C. Teuscher. 2002. Interacting quantitative trait loci control loss of peripheral tolerance and susceptibility to autoimmune ovarian dysgenesis after day three thymectomy in mice. J. Immunol. 169:1640-1646. Potter, M.R., S.R. Rittling, D.T. Denhardt, R.J. Roper, J.H. Weis, C. Teuscher, and J.J. Weis. 2002. Role of osteopontin in murine Lyme arthritis and host defense against Borrelia burgdorferi. Infect. Immun. 70:1372-1381. Roper, R.J., J.J. Weis, B.A. McCracken, C.B. Green, Y. Ma, K.S. Weber, D. Fairbairn, R. J. Butterfield, M.R. Potter, J.F. Zachary, R.W. Doerge, and C. Teuscher. 2001. Genetic control of susceptibility to experimental Lyme arthritis is polygenic and exhibits consistent linkage to multiple loci on chromosome 5 in four independent mouse crosses. Genes Immun. 2:388397. Butterfield, R.J., E.P. Blankenhorn, R.J. Roper, J.F. Zachary, R.W. Doerge, and C. Teuscher. 2000. Identification of genetic loci controlling the characteristics and severity of brain and spinal cord lesions in experimental allergic encephalomyelitis. Am. J. Path. 157:637-645. Roper, R.J., J.S. Griffith, C.R. Lyttle, R.W. Doerge, A.W. McNabb, R.E. Broadbent, and C. Teuscher. 1999. Interacting quantitative trait loci control phenotypic variation in murine estradiol-regulated responses. Endocrinology 140:565-561. Butterfield, R.J., E.P. Blankenhorn, R.J. Roper, J.F. Zachary, R.W. Doerge, J.D. Sudweeks, J. Rose, and C. Teuscher. 1999. Genetic analysis of disease subtypes and sexual dimorphisms in mouse experimental allergic encephalomyelitis (EAE): relapsing/remitting and monophasic remitting/nonrelapsing EAE are immunogenetically distinct. J. Immunol. 162:3096-3102.

RANDALL J. ROPER, PH.D.

PUBLICATIONS AND PRESENTATIONS (CONTINUED)
Journal Articles (Continued): Weis J., B.A. McCracken, Y. Ma, D. Fairbairn, J. Weis, R.J. Roper, J.F. Zachery, R.W. Doerge, and C. Teuscher. 1999. Identification of quantitative trait loci governing arthritis severity and humoral responses in the murine model of Lyme disease. J. Immunol. 162:948-956.

Roper, R.J., R.W. Doerge, S.B. Call, K.S.K. Tung, W.F. Hickey, and C. Teuscher. 1998. Autoimmune orchitis, epididymitis, and vasitis are immunogenetically distinct lesions. Am. J. Path. 152:1337-1345. Butterfield, R.J., J.D. Sudweeks, E.P. Blankenhorn, R.G. Korngold, J.C. Marini, J.A. Todd, R.J. Roper, and C. Teuscher. 1998. New genetic loci that control susceptibility and clinical symptoms of experimental allergic encephalomyelitis in inbred mice. J. Immunol. 161:18601867. Pitt, W.G., M.O. McBride, J.K. Lunceford, R.J. Roper, and R.D. Sagers. 1994. Ultrasonic enhancement of antibiotic action on gram-negative bacteria. Antimicrob. Agents Chemother. 38:2577-2582. Invited Talks: (Condensed from ~40) Roper, R.J. 2010. Altered mandibular development in Down syndrome linked to modified expression of non-trisomic genes. Department of Statistics, Purdue University, (West Lafayette, IN). Roper, R.J. 2009. Of mice and men: what a mouse can teach us about Down syndrome. International Mosaic Down Syndrome Society (Cincinnati, OH). Roper, R.J., M. Stanley, S. Stone, N. Duvall, C. Davis, C. N. Billingsley, J. Blazek, J. Allen, and J. VanHorn. 2009. Mice and men: face to face with Down syndrome. IUPUI Research Day (Indianapolis, IN). Roper, R.J. 2009. El ratn y el sndrome de Down (The mouse and Down syndrome) Cuenta Conmigo (Meeting for Hispanic Parents of children with Down syndrome) Down Syndrome Indiana (Indianapolis, IN). Roper, R.J. 2009. Developmental and genetic origins of neural crest deficit in Down syndrome mice. Department of Computer Science, Brigham Young University (Provo, UT). Roper, R.J. 2008. Using mouse models of Down syndrome to investigate traits and potential therapies, Down Syndrome Indiana (Indianapolis, IN). Roper, R.J. 2008. The ups and downs of Down syndrome mouse models, Indiana Branch, American Association of Laboratory Animal Sciences (Indianapolis, IN). Roper, R.J. 2008. Neural crest deficit in Down syndrome mice associated with Sonic hedgehog deficiency, Department of Medical and Molecular Genetics, Indiana University School of Medicine (Indianapolis, IN). Roper, R.J. 2007, Neural crest deficit in Down syndrome mice. Department of Statistics, Purdue University, (West Lafayette, IN). Roper, R. J. 2007. Neural crest deficit in Down syndrome mice. 46th Annual Midwest Developmental Biology Meeting (Chicago, IL). Roper, R. J. 2006. Neural crest deficit in Down syndrome mice. Department of Cellular and Integrative Physiology, IUPUI (Indianapolis, IN) Roper, R.J. 2006. Neural crest deficit in craniofacial precursors of Down syndrome mice. The 20th International Mouse Genome Conference. (Charleston, SC).

RANDALL J. ROPER, PH.D.

PUBLICATIONS AND PRESENTATIONS (CONTINUED)

Abstracts: (Condensed from >40) Blazek J.D., B. Harman, A. Gaddy, S. Deitz, and R.J. Roper. 2010. NFAT expression and localization in the mandibular precursor of Ts65Dn Down syndrome mice. GENETICS 2010: Model Organisms to Human Biology (Boston, MA) (Poster 75A, p. 23). Billingsley, C.N., J.A. Allen, J.D. Blazek, D.D. Baumann, A. Newbauer, A. Darrah, M. Clement, R.W. Doerge, and R.J. Roper. 2010. Altered mandibular development in Down syndrome mouse model linked to modified expression of non-trisomic homeobox genes. GENETICS 2010: Model Organisms to Human Biology (Boston, MA) (Poster 123A, p. 23). Roper R.J., M. Stanley, N. Duvall, N. Shepherd, S. Stone, C. Davis, C.N. Billingsley, and J. Blazek. 2009. The neural crest basis for craniofacial abnormalities associated with Down syndrome. 59th Annual Meeting American Society for Human Genetics, Honolulu, HI (Poster 2402). Blazek J., A. Gaddy, R. Meyer, J. Li and R.J. Roper. 2009. Altered bone formation and homeostasis in the Ts65Dn mouse model of Down syndrome. 23rd Annual International Mammalian Genome Conference, La Jolla, CA. (Poster 113, p. 125). Duvall N., S.B. Stone, C. Davis, M. Stanley, and R. Roper. 2009. A retrospective analysis of comorbid traits associated with craniofacial dysmorphology in infants with Down syndrome. 48th Annual Midwest Developmental Biology Meeting, Iowa City, IA (Talk 3, p. 10). Blazek J., A. Newbauer, and R.J. Roper. 2009. Developmental Delay in the Ts65Dn mouse model of Down syndrome. 48th Annual Midwest Developmental Biology Meeting, Iowa City, IA (Talk 19, p. 19). Deitz S., J. Allen, J. VanHorn, and R.J. Roper. 2008. Genetic basis of neural crest deficit in Down syndrome mice. 22nd International Mammalian Genome Conference (Prague, Czech Republic). (Poster 103, p. 126). Roper R.J., J.F. VanHorn, C.C. Cain, and R.H Reeves. 2007. Neural crest deficit in Down syndrome mice associated with Sonic hedgehog mitotic deficiency. Expert Workshop on the Biology of Chromosome 21 Genes: Towards the Gene-Phenotype Correlations in Down syndrome (Washington, D.C.). (Abstract 24 p. 80)

CURRICULUM VITAE NAME David Gordon Skalnik

EDUCATION UNDERGRADUATE: 1977-1981 University of California, Santa Barbara B.A. - Biochemistry and Molecular Biology, GPA 3.72 GRADUATE: 1981-1987 Department of Biological Sciences, Stanford University. Ph.D.- "Functional analysis of the membrane domain of HMG-CoA reductase". Thesis advisor: Robert Simoni, Ph.D.

POSTDOCTORAL: 1987-1991 Harvard Medical School (Children's Hospital). Project concerned the regulation of gene expression in phagocytes. This work was performed in the laboratory of Dr. Stuart Orkin, M.D., Howard Hughes Medical Institute ACADEMIC APPOINTMENTS 1991-1996 Assistant Professor of Pediatrics and Biochemistry & Molecular Biology Wells Center for Pediatric Research Indiana University School of Medicine 1996-2001 1992-1996 1997-present 2001-2010 2006-present 2010 present 2010 present Associate Professor of Pediatrics and Biochemistry & Molecular Biology, Indiana University Associate member - Graduate Faculty, Indiana University Full member - Graduate Faculty, Indiana University Full Professor of Pediatrics and Biochemistry & Molecular Biology, Indiana University Special appointment to the Graduate Faculty of Purdue University Professor of Biology, Indiana University Purdue University Indianapolis School of Science Associate Dean for Research and Graduate Education, Indiana University Purdue University Indianapolis School of Science

PROFESSIONAL ORGANIZATIONS American Association for the Advancement of Science American Society of Hematology American Society of Microbiology Society for Pediatric Research DNA Methylation Society American Association for Cancer Research American Society for Biochemistry and Molecular Biology 1

HONORS and AWARDS 1978-1981 Dean's list at U.C. Santa Barbara 1981 Graduated with high honors from U.C.S.B. with major of Biochemistry/Molecular Biology 1981-1986 NIH pre-doctoral Traineeship recipient - Stanford University 1987-1990 Awarded NIH (grant # 1F32 HL07607-01 BI-6) and American Cancer Society (grant #PF-3054) postdoctoral fellowships. ACS grant was activated. Total award- $68,150 1992-1995 American Cancer Society Junior Faculty Award 2004 Recipient of Trustee Teaching Award for excellence in teaching at Indiana University School of Medicine HISTORY of LABORATORY PERSONNEL Lab Member Title Years Elizabeth Eklund, M.D. Post-Doc 1991-1993 Darlene Barnard Andy Esche Wen Luo, B.S. Beverly Pero, M.S. Seung-won Lee, Ph.D. Elizabeth Ghareghaghi -Schnell Mary Maze, B.S. Shannon Hawkins, B.S. Xuemei Ou, M.S. Kui Shin Voo, Ph.D. Diana Catt, Ph.D. Britta Jacobsen, B.S. Denver Health Sci. Cntr. Technician Technician 1991-1993 1991

Current Position Professor of Medicine, Northwestern University Technician, Eli Lilly Technician, State of Indiana

Ph.D. Student 1992-1996 Senior Scientist, Ligand Pharmaceuticals, San Diego, CA Technician Post-Doc Post-Doc Technician 1992-1993 1993-1994 1993-1994 1994-1995 Technician, Eli Lilly Post-Doc, Purdue University Post-Doc, Vienna, Austria Dentist

M.D., Ph.D. 1994-2001 Assistant Professor, Baylor Univ. Combined Degree Student M.S. Student Post-Doc Post-Doc 1994-1997 Technician, Eli Lilly

1994-2000 1994-1998

Instructor, MD Anderson Asst. Scientist, I.U. Dental School Assistant Professor,

Ph.D. Student

1995-1999

Diana Carlone, Ph.D. Post-doc Instructor, Harvard Medical School Paula Ladd, M.S. Paula Ladd, M.S. 2 Technician

1996-2002 1998-1999

Res. Assistant Prof., U. Conn.;

Ph.D. Student 1999-2004 Univ. Washington Post-doc

Donghui Kan, M.S. Angela Nevin Jeong-Heon Lee, Ph.D.

Technician Technician Post-doc

1999-1999 Technician, Univ. Chicago 2000-2001 Research Scientist, Eli Lilly 2001-present

Suzanne Hart (Young), B.S.Ph.D. Student 2001-2005 Post-doc, IU Erika Zimmerman, B.S. Mike Bruno, B.S. Jill Sergesketter, B.S. Audrey Wehr, B.S. Technician Technician 2002-present 2002-2003

Ph.D. Student 2002-2007 Post-doc, MD Anderson Cancer Ph.D. Student 2002-2003 Resident, IU/Riley Hospital Ph.D. Student 2003-2008 Post-doc, IU

Rajarajeswari Muthukrishnan, B.S. Courtney Tate, B.S. Hitesh Kathuria, Ph.S.

Ph.D. Student 2004-2009 Post-doc, FBI forensic labs Post-doc 2005-2006, Assistant Professor, IU East (Richmond)

Patricia Pick-Franke, B.S. M.S. Student 2008-2010 Jyothi Mahadevan, B.S. Ph.D. Student 2010-present

Summer students/MSMS students: Heather McDaniels 1993 Jennette Brister (MEDIC-B) 1998 Melissa Rutigliano 1998, 1999 Allyson Fisher (MEDIC-B) 2000 Todd Bertrand, 2002 Andrea Shin 2003, 2004 Stuart Vik, 2005 Mica Kleber (MSMS), 2006 Gena Mooneyham (MSMS), 2006 Chong-Yang Lee, 2006 Amaris Evans, 2009 3

Joanna Campodonico, 2009

TEACHING B500, Introductory Biochemistry (3 credits). 1992-2009. Course director for 10 years. This lecture course is designed for non-Biochemistry Ph.D., non-degree graduate students. Biotechnology Certificate students, and Masters of Science in Medical Science (MSMS) students. Enrollment is ~50. B817, Eukaryotic Cell Biology (2-3 credits). 1992-2006. This graduate course was a component of the core curriculum for the Department of Biochemistry & Molecular Biology and covers a wide range of cell biology topics. I presented from one to 6 hours of lectures each year. Enrollment was 20-25. X604, Concepts in Health and Disease (Problem based learning). Served as facilitator for small groups of first year medical students. 1992-1995, 1998-1999. Q620, Human Cytogenetics (2002-present) Present a single lecture on genomic imprinting. Enrollment ~20. G865, Molecular Biology (3 credits), 2004-2006. This graduate course was a component of the core curriculum for the Department of Biochemistry & Molecular Biology and covered a wide range of molecular biology topics. I presented lectures on chromatin structure and epigenetics. Enrollment was ~25. B855, Research. I have directed the dissertation research of nine graduate students in the Biochemistry program. I also present an annual lecture to the pediatric hematology/oncology fellows on the topic of globin gene regulation and chromatin structure. PROFESSIONAL ACTIVITIES 1993-97, 1999-2005 Member of Graduate Program Admissions Committee for the Department of Biochemistry & Molecular Biology. 1993, 1994, 1998 1994-1997 1994-1997 1995-2001 4 Organized scientific retreats for IUSM hematopoiesis researchers Member of the Wells Center for Pediatric Research Advisory Committee. Co-chair of Education and Training Committee, Indiana University Cancer Center. Member of Institutional Biosafety Committee.

1997-2001 1997-2000 2001-2005 1991-1998

Alternate member of Institutional Review Board. Chair of the bridge funding/scientist track evaluation committee for the Wells Center Member of the Institutional Animal Care and Use Committee Operated an oligonucleotide synthesis core facility for the use of Wells Center and campus researchers. This facility produced approximately 5,000 oligonucleotides. Member of the Cancer Biology Training Program steering committee. Member of the steering committee for the Masters of Science in Medical Science (MSMS) program. Member of the admissions committee for the MSMS program. Member of IUSM biostatistics task force. Member of IUSM animal use task force. Member of the Indiana University Cancer Center (regulation of cell growth program). Member of search committee for Director of the Wells Center for Pediatric Research, IUSM. Member of search committee for Director of Pediatric Hematology/Oncology, IUSM. Member of faculty search committee for Developmental Cardiology research group within the Wells Center (Loren Field Director), IUSM. Member of faculty search committee in the area of chromosome structure and function for the Dept. of Medical & Molecular Genetics, IUSM. Organizer of the DNA-binding protein club, which met monthly to present/discuss current research on campus. Approximately a dozen laboratory participated in this series. Member of the Biotechnology training program steering committee. Faculty mentor for IUSM medical students. Member of mentoring committee for Dr. Virginia Meade, IUSM neonatology fellow.

2000-present 2001-present 2002-present 2002-2003 2003-2004 ~1998-present 1999 1999 2000-2002

2003

1992-2001

2001-present 2002-2004 2002-2004

2004-2006 2004-present 2005-present 2005-present 2005 2006-present 2006-present 2006-present 2006 2006-present 2007-2008 2007-present 2008-present 2008-2009 2009 2009-2010 2009-present 2010-present 2011-present

Chair, Laboratory Animal Resource Center (LARC) Advisory Committee Member of Indiana University Immunobiology Center Member of Advisory Board for IU Embryonic Stem cell core facility Member of Advisory Board for IU DNA Sequencing core facility Grant reviewer for IU seed grant program for NIH Roadmap initiative. Member of Pediatric Pulmonary faculty search and screen committee. Member of the IUPUI Research Support Fund Grant review panel. Member of review panel for Simmons Clinical Pilot Grants Member of review panel for IU Cancer Center and Purdue Cancer Center Pilot Grants Member of the IU Center for Regenerative Biology and Medicine Member of Educational Quality Measures Committee at IUSM (subcommittee of the data driven decision directive) Member of advisory core for IU mouse cytogenetics core facility Member of executive committee for IU immunobiology training program Member of Molecular Oncology faculty search and screen committee, dept of pediatrics, section of heme/onc Member of IU Proteomics task force committee Member of faculty search and screen committee for Dept of Biochemistry and Molecular Biology, IUSM Member of grant review panel for IUPUI Developing Diverse Researchers with Investigator Expertise (DRIVE) Member of IUPUI Conflict of Interest committee Chair, IUPUI School of Science Graduate Affairs committee

Member of numerous student thesis committees: Wen Luo Ph.D. 1992-1996 Shannon Hawkins M.D./Ph.D. 1994-2001 6

Xuemei Ou Xin Zhang Hu Hu Kent Hansen Edward Gize Britta Jacobsen Melissa Limp-Foster Marianne Price Pasutha Thunyakitpisal Yaohui.Xu Randy Shen Paula Ladd Xiaodong Xie Suzanne Hart (Young) Yu Li Jill Sergesketter (Butler) Audrey Wehr Qi Xu Luke Dannenberg Yahaira Naaldijk Marin Garcia Courtney Tate Raji Muthukrishnan Jeff Altenburg Jennifer Rodenberg Ph.D. Sunyong Tang Rachel Mullen Marcus Breese Kiho Lee (Purdue) Erin Breese Eric Benson Sirisha Pochareddy Ryan Widau Sowmya Jairam Lakshmi Reddy Palam Patricia Pick-Franke Raleigh Malik Soyoung Park Jyothi Mahadevan

M.S. Ph.D. M.S. M.D.,Ph.D. M.D., Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. M.S. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. M.S. (Purdue) Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. M.D./Ph.D. Ph.D. M.D., Ph.D. M.D., Ph.D. Ph.D. Ph.D. Ph.D. Ph.D. M.S. Ph.D. Ph.D. Ph.D.

1994-1997 1992-1998 1994-1996 1995-1999 1995-1999 1995-1999 1997-2001 1997-2001 1998-2000 1999-2001 1999-2001 1999-2004 1999-2001 2001-2005 2001-2004 2002-2007 2003-2004 2003-2004 2003-2006 2002-2008 2003-2006 2004-2009 2003-2008 2004-2007 2005-2008 2005-2010 2006-2010 2002-present 2006-2010 2006-2006 2006-2007 2006-2010 2006-2010 2007-present 2008-present 2008-2010 2009-present 2009-present 2010-present

Faculty mentoring committees: Ed Chan, M.D. 2004-2007 Tony Firulli, Ph.D 2004-2006 David Ingram, M.D. 2002-2006 Becky Chan, M.D., Ph.D. 2004-present Joeseph Ruiz, Ph.D. 2002-2006 Kathey Bethin, M.D. 2004-2007 Brenda Grimes, Ph.D. 2004-present Lindsey Mayo, Ph.D. 2007-present Shreevrat Goenka, Ph.D. 2007-present Manjari Mazumdar, Ph.D. 2010-present 7

Manuscript reviewer: Blood Genomics Gene Oncogene, European Journal of Hematology Journal of Biological Chemistry European Journal of Biochemistry Biochemica Biophysica Acta Cancer Research Journal of Leukocyte Biology Molecular and Cellular Biology Experimental Cell Research Infection and Immunity Human Mutation Cloning and Stem Cells Nature Structural and Molecular Biology Grant Reviewer Ad hoc grant reviewer: Veterans Administration Ad hoc grant reviewer: National Science Foundation Ad hoc grant reviewer: Cancer Research Fund, United Kingdom Ad hoc grant reviewer: National Institute of Child Health and Development Member of Breast Cancer Study Section, Dept. of the Army Member of Ovarian Cancer Study Section, Dept. of the Army Member of Prostate Cancer Study Section, Dept. of the Army Member of National Cancer Institute program project grant site visit review panel. Member of Komen Foundation breast cancer review panel, January 2006. Member of Breast Cancer Study Section, Dept of the Army, August 2006. Member of National Science Foundation Review Panel, April 2007. Member of Breast Cancer Study Section, Dept. of the Army, August 2007. Ad hoc reviewer, Wellcome Trust, 2007. Reviewer for Simmons Pilot Awards, IU cancer center, 2008. Chair and grant reviewer, special emphasis panel for ETTN/NIH. 8

Invited Seminars: Ball State University, 10/4/91, Regulation of gene expression in phagocytes FASEB meeting, Anaheim, CA, 4/9/92, Targeting of transgene expression to monocyte/macrophages Dept. of Pediatrics, IUSM, 2/8/93, Regulation of gene expression in phagocytes Northwest Medical Center, Gary, 4/22/94, Regulation of gene expression in phagocytes Wells Center/Walther/Heme-Onc combined seminar series, IUSM, 11/6/96, Regulation of gene expression in phagocytes University of Tennessee, Dept. of Biochemistry, Memphis, 3/97, Regulation of gene expression in phagocytes Public Health Research Institute, New York, 9/23/97, Regulation of gene expression in phagocytes Leukemia Society of America Symposium, Houston, 10/7/97, Role of CCAAT displacement protein in the regulation of myeloid cell gene expression. Dept of Biology, Purdue (IUPUI), 10/1/99, Regulation of gene expression in phagocytes Dept. of Biochemistry & Molecular Biology, IUSM, 1/17/2000, Cloning and functional analysis of CpG binding protein. University of Arizona, Genetics Program, Tuscon, 9/6/2000, Cloning and functional analysis of CpG binding protein. Dept. of Pediatrics, IUSM, 12/11/2000, Cloning and functional analysis of CpG binding protein University of Pennsylvania, 12/17/02, Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Dept. Pediatrics, IUSM, 2/10/03, Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Oregon Health Sciences University, 4/9/03, Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Indiana University School of Medicine (job interview for Chair position for the Department of Biochemistry & Molecular Biology), 5/27/03, Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Purdue University, Department of Animal Sciences. 11/2/04. Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure. 9

Indiana University (Bloomington), Medical Sciences Program. 9/19/05. CpG binding protein: a critical epigenetic regulator of cytosine methylation and the histone code. Indiana University, Department of Pediatrics. 1/12/06. CpG binding protein, a critical regulator of cytosine methylation and the histone code. Indiana University, Department of Anatomy and Cell Biology. 1/23/07. SETting the stage for epigenetic regulation. Indiana University, Department of Medical and Molecular Genetics. 2/13/08. SETting the stage for epigenetic regulation. Purdue University, Department of Biochemistry, 2/19/2008. SETting the stage for epigenetic regulation. Indiana University, Department of Pharmacology and Toxicology. 9/9/08. CXXC finger protein 1: A critical regulator of mammalian epigenetics. Syracuse University, Department of Biology. 11/14/08. CXXC finger protein 1: A critical regulator of mammalian epigenetics. Indiana University, Pediatric Research Faculty Seminar. 03/06/2009. CXXC finger protein 1: SETting the stage for epigenetic regulation. Ohio State University regional meeting on the Role of Epigenetics in Cancer: Mechanisms and Applications in Therapy. 11/09/2009. CXXC finger protein 1: SETting the stage for epigenetic regulation. Indiana University-Purdue University Indianapolis, Dept of Biology. (job interview for Chair position for the Biology Department) 02/17/10 Genetic and biochemical analyses of CXXC finger protein 1, an epigenetic regulator of vertebrate development. Indiana University Medical Sciences Program (Bloomington). 04/26/10. Genetic and biochemical analyses of CXXC finger protein 1, an epigenetic regulator of vertebrate development. FASEB Summer Research Conferences. Biological methylation: form DNA to histones and beyond. Genetic and biochemical analyses of CXXC finger protein 1, an epigenetic regulator of mammalian development. June 6-11, 2010, Carefree Resort, AZ. FUNDING HISTORY National Leukemia Association (Kirby Foundation Scholarship) Regulation of Gene Expression in Myeloid Cells Total direct costs: $18,000 1/1/91 to 12/31/92 Principal Investigator - David Skalnik Basic Science Research Grant (IUSM) Regulation of Gene Expression and Development in Phagocytes Total direct costs: $20,573 10

1/1/92 to 12/31/92 Principal Investigator - David Skalnik Riley Memorial Association Grant-in Aid #RMA 92-1 Regulation of Gene Expression in Myelomonocytic Cells Total direct costs: $14,951 7/1/92 to 6/30/93 Principal Investigator - David Skalnik (Award declined due to grant overlap) National Cancer Institute Program Project Grant (David Williams, program director) #PO1 CA59348 Program title: Dose Intensification by Gene Transduction Section title: Treatment of Neuroblastoma Using the Transgenic Mouse Model and Immune Modulation. Total direct costs for project: $2,257,441 Total direct costs for section: $468,885 10/1/92 to 9/30/96 Randy Hock and David Skalnik, Co-Principal Investigators National Institutes of Health - FIRST award #R29 CA58947 Title: Regulation of Gene Expression in Phagocytes Total direct costs: $350,000 3/1/93 to 2/30/98 Principal Investigator - David Skalnik American Cancer Society Junior Faculty Award #421 Title: Regulation of Gene Expression in Myelomonocytic Cells. Total direct costs: $90,500 7/1/92 to 6/30/95 Principal Investigator - David Skalnik National Institutes of Health Clinical Investigator Development Award (awarded to Dr. Elizabeth Eklund while a postdoctoral fellow in my laboratory) #K08 HL02915 Title: Tissue specific transcriptional regulation of gp91-phox Total direct costs: $238,402 8/1/93 to 7/31/96 Mentor - David Skalnik Riley Memorial Association Grant-in-Aid #RMA 95-2 Title: Identification and Cloning of Proteins That Interact with CCAAT Displacement Protein. Total direct costs: $23,972 7/1/95 to 6/30/96 Principal Investigator - David Skalnik 11

Arthritis Foundation Research Award Title: Transcriptional regulation of gp91-phox, a respiratory burst oxidase component Total direct costs: $208,275 7/1/96 to 6/30/99 Principal Investigator - David Skalnik American Heart Association Grant-in-aid Title: Regulation of lineage-specific gene expression in phagocytes Total Direct Costs: $120,000 7/1/96 to 6/30/99 Principal Investigator - David Skalnik (Award declined due to grant overlap) Arthritis Foundation Postdoctoral Fellowship (to support Dr. Kui Shin Voo) Title: Purification and cloning of a transcription factor involved in macrophage specific gene expression Total Direct Costs: $51,500 7/1/96 to 6/30/98 Mentor: David Skalnik National Institutes of Health - F32 HD07991 Postdoctoral Fellowship (to support Dr. Diana Carlone) Title: Ovarian Expression and Regulation of HAF-1 in the Rat Total Direct Costs: $25,000 12/1/96 to 8/25/97 Mentor: David Skalnik American Heart Association Postdoctoral Fellowship (to support Dr. Diana Carlone) Title: Expression and Functional Analysis of Hematopoietic Associated Factor Total Direct Costs: $41,000 7/1/97 to 6/30/99 Mentor: David Skalnik National Institutes of Health - RO1 competitive renewal of R29 grant RO1 CA58947-06 Title: Regulation of Gene Expression in Phagocytes Total Direct Costs: $701,937 3/1/98 to 1/31/03 Principal Investigator - David Skalnik Indiana University Cancer Center Development Award - Pilot Projects Title: AP endonuclease expression and myeloid leukemia sensitivity to Chemo/Radiotherapy Total Direct Costs: $10,000 Funds available to Skalnik: $3,300 1998 12

Principal Investigators: Kent Robertson and David Skalnik National Institutes of Health - RO1CA76643-01A1 Title: APE expression/leukemia response to chemo-radiotherapy Total direct costs: $813,095 Portion available to Skalnik: ~$250,000 7/1/98 to 6/30/02 Principal Investigator: Kent Robertson Co-investigators: Mark Kelley and David Skalnik National Institutes of Health, PO1 Title: Role of Rac2 in Hematopoiesis Total direct costs requested: 4/22/02 to 3/31/07 Total direct costs: ~$5,000,000 Portion available to Skalnik: ~$1,000,000 Principal Investigator: David Skalnik Indiana University School of Medicine: Core utilization grant Identification of proteins that interact with CpG binding protein and production of protein crystals containing this factor's DNA binding domain. PI: Skalnik 7/1/02 to 6/30/03 Total direct costs: $7,273 Indiana University School of Medicine Core utilization grant Creation of a conditional allele for CpG binding protein in mice. PI: Skalnik 7/1/03 to 6/30/04 Total direct costs: $10,000 National Science Foundation Title: Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Principal Investigator: David Skalnik 5/1/04 to 4/30/07 Total direct costs: $340,000 American Heart Association Pre-doctoral Fellowship (to support Suzanne Young) Title: Role of CpG binding protein in myeloid, erythroid and cardiac development. Total Direct Costs: $49,000 7/1/03to 6/30/05 Mentor: David Skalnik Indiana University School of Medicine: Core utilization grant Analysis of gene expression patterns in murine ES cells lacking CpG binding protein Principal Investigator: David Skalnik 7/1/05 to 6/30/06 Total direct costs: $10,000 Indiana University School of Medicine Core utilization grant 13

In vivo analysis of the developmental potential of CpG binding protein-null and rescued embryonic stem cells. Principal Investigator: David Skalnik 7/1/06 to 6/30/07 Total direct costs: $9230 National Institutes of Health, RO1 PI: Feng Zhou Co-investigator (10%): David Skalnik Epigenetic effects of alcohol exposure on embryonic development. 9/1/06 to 8/30/2010 National Science Foundation Title: Role of CpG binding protein in the regulation of cytosine methylation and chromatin structure Principal Investigator: David Skalnik 6/1/07 to 5/31/10 Total direct costs: $321,000 IUPUI Research Support Fund Grant Title: Role of CpG binding protein in hematopoiesis. Principal Investigator: David Skalnik 7/1/07 to 6/30/08 $35,000 IUPUI Research Support Funds Grant Title: Genomic targeting of mammalian Set1 methyltransferase complexes. Principal Investigator; David Skalnik 7/1/09 to 6/30/10. $55,000 Biomedical Research Grant (IUSM). Title: Role of Cfp1 in hematopoietic stem cell function. Principal Investigator: David Skalnik 7/1/09 to 6/30/10 $40,000 Core utilization grant, IUSM Title: Genomic targeting of Set1 histone methyltransferase complexes Principal Investigator: David Skalnik 7/1/10 to 6/30/12 $6,600 CURRENTLY ACTIVE

PUBLICATIONS 1. Skalnik, D.G., Brown, D.B., Brown, P.B., Hardeman, E.C., Friedman, R.C., Schimke, R.T., and Simoni, R.D. (1985) Mechanisms of overaccumulation of 3-

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hydroxy-3-methylglutaryl coenzyme A reductase in three compactin resistant cell lines. Journal of Biological Chemistry 260, 1991-1994. PMID: 3838302 2. 3. Skalnik, D.G., and Simoni, R.D. (1985) The nucleotide sequence of syrian hamster HMG-CoA reductase cDNA. DNA 4, 439-444. PMID:3841506 Klionsky, D.J., Skalnik, D.G., and Simoni, R.D. (1986) Differential translation of the genes encoding the proton-translocating ATPase of Escherichia coli. Journal of Biological Chemistry 261, 8096-8099. PMID: 2873137 Skalnik, D.G., Narita,H., Kent, C., and Simoni, R.D. (1988) The membrane domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase confers endoplasmic reticulum localization and sterol-regulated degradation onto galactosidase. Journal of Biological Chemistry 263, 6836-6841. PMID: 2834394 Skalnik, D.G., and Orkin, S.H. (1990) A rapid method for characterizing transgenic mice. Biotechniques 8, 34. PMID: 2322450 Skalnik, D.G., Dorfman, D.M., Perkins, A.S., Jenkins, N.A., Copeland, N.G.,and Orkin, S.H. (1990) trans. Assoc. Am. Physicians 103, 129-136. PMID: 2132524 Skalnik, D.G., Dorfman, D.M., Williams, D.A., and Orkin, S.H. (1991) Restriction of neuroblastoma to the prostate gland in transgenic mice. Molecular and Cellular Biology 11, 4518-4527. PMID: 1652058 Skalnik, D.G., Dorfman, D.M., Perkins, A., Jenkins, N., Copeland, N., and Orkin, S.H. (1991) Targeting of transgene expression to monocyte/ macrophages by the gp91-phox promoter and consequent histiocytic malignancies. Proceedings of the National Academy of Science, USA, 88, 8505-8509. PMID:1656446 Skalnik, D.G., Strauss, E.C., and Orkin, S.H. (1991) CCAAT displacement protein as a repressor of the myelomonocytic-specific gp91-phox promoter. Journal of Biological Chemistry 266, 16736-16744. PMID: 1885602 Neufeld, E.J., Skalnik, D.G., Lievens, P.M.J., and Orkin, S.H. (1992) Human CCAAT displacement protein is homologous to the Drosophila homeoprotein, cut. Nature Genetics 1, 50-55. PMID: 1301999 Skalnik, D.G., and Neufeld, E.J. (1992) Sequence-specific binding of HMG-I(Y) to the promoter of the gp91-phox gene. Biochemical and Biophysical Research Communications 187, 563-569. PMID: 8422257 Newburger, P.E., Skalnik, D.G., Hopkins, P.J., Eklund, E.A., and Curnutte, J.T. (1994) Mutations in the promoter region of the gene for gp91-phox in X-linked chronic granulomatous disease with decreased expression of cytochrome b558. Journal of Clinical Investigation 94, 1205-1211. PMID: 8083361 Eklund, E.A., Lee, S.W., and Skalnik, D.G. (1995) Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1. Gene 155, 231235. PMID: 7721096

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Eklund, E.A., and Skalnik, D.G. (1995) Characterization of a gp91-phox promoter element that is required for interferon gamma induced transcription. Journal of Biological Chemistry 270, 8267-8273. PMID: 7713934 Luo, W., and Skalnik, D.G. (1996) CCAAT displacement protein competes with multiple transcriptional activators for binding to four sites in the proximal gp91phox promoter. Journal of Biological Chemistry 271, 18203-18210. PMID: 8663528 Luo, W., and Skalnik, D. G. (1996) Interferon regulatory factor-2 directs transcription from the gp91-phox promoter. Journal of Biological Chemistry 271, 23445-23451. PMID: 8798551 Eklund, E. A., Luo, W., and Skalnik, D. G. (1996) Characterization of three promoter elements and cognate DNA-binding protein(s) necessary for interferongamma induction of gp91-phox transcription. Journal of Immunology 157, 2418-2429. PMID: 8805641 Pattison, S., Skalnik, D.G., and Roman, A.(1997) CCAAT displacement protein, a candidate regulator of differentiation specific human papillomavirus gene expression, binds a negative regulatory element within the 5' end of the human papillomavirus type 6 long control region. Journal of Virology 71, 2013-2022. PMID: 9032333 Voo, K.S.*, and Jacobsen, B.M*. (1998) Rapid resuspension of pelleted bacterial cells for miniprep plasmid DNA isolation. Biotechniques 24, 240-243. *Students in Skalnik lab. PMID: 9494723 Teerawatanasuk, N., Skalnik,D.G., and Carr, L.G. (1999) CCAAT displacement protein (CDP/Cut) binds a negative regulatory element in the human tryptophan hydroxylase gene. Journal of Neurochemistry. 72, 29-39. PMID: 9886051 Ou, X., Pollock, J., Dinauer, M.C., Gharehbaghi-Schnell, E., and Skalnik, D.G. (1999) Identification and functional characterization of the murine Rac2 gene promoter. DNA and Cell Biology. 18, 253-263. PMID: 10098607 Voo, K.S., and Skalnik, D.G. (1999) Elf-1 and PU.1 induce gp91-phox promoter activity via an element mutated in a subset of chronic granulomatous disease patients. Blood. 93, 3512-3520. PMID: 10233904 Catt, D., Hawkins, S., Roman, A., Luo, W., and Skalnik, D.G. (1999) Overexpression of CCAAT displacement protein represses the promiscuously active proximal gp91-phox promoter. Blood. 94, 3151-3160. PMID: 10556202 Catt, D., Luo, W., and Skalnik, D.G. (1999) DNA-binding properties of CCAAT displacement protein cut repeats. Cellular and Molecular Biology 45, 1149-1160. PMID: 10643964 Jacobsen, B.M., and Skalnik, D.G. (1999) YY1 binds five cis-elements and activates the myeloid cell-restricted gp91-phox promoter.Journal of Biological Chemistry 274, 29984-29993. PMID: 10514482

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Voo, K.S., Carlone, D.L., Jacobsen, B.M., Flodin, A., and Skalnik, D.G. (2000) Cloning of a mammalian transcriptional activator that binds unmethyalted CpG motifs and shares a CXXC domain with DNA methyltransferase, human trithorax, and methyl CpG-binding domain protein 1. Molecular and Cellular Biology 20, 2108-2121. PMID: 10688657 Hawkins, S.M., Kohwi-Shigematsu, T., and Skalnik, D.G. (2001) The matrix attachment region-binding protein SATB1 interacts with multiple elements within the gp91-phox promoter and is down regulated during myeloid differentiation. Journal of Biological Chemistry 276, 44472-44480. PMID: 11577075 Carlone, D.L., and Skalnik, D.G. (2001) CpG binding protein is necessary for early development. Molecular and Cellular Biology 21, 7601-7606. PMID: 11604496; PMCID: 99931. Lee, J.-H., Voo, K.S., and Skalnik, D.G. (2001) Identification and characterization of the DNA-binding domain of CpG binding protein. Journal of Biological Chemistry 276, 44669-44676. PMID: 11572867 Lee, J.-H., and Skalnik, D.G. (2002) CpG binding protein is a nuclear matrix- and euchromatin-associated protein localized to nuclear speckles containing human trithorax: Identification of nuclear matrix targeting signals. Journal of Biological Chemistry 277, 42259-42267. PMID: 12200428 Carlone, D.L., Hart, S.H.L., Ladd, P., and Skalnik, D.G. (2002) Cloning and characterization of the gene encoding the murine homologue of CpG binding protein. Gene 295, 71-77. PMID: 12242013 Ladd, P.D., Wilson, D.M., Kelley, M.R., and Skalnik, D.G. (2002) Identification of the human HEX1/hEXO1 gene promoter and characterization of elements responsible for promoter activity. DNA Repair 109, 1-12.PMID: 12531389 Lee, J.-H., Hart, S.H.L., and Skalnik, D.G. (2004) Histone deacetylase activity is required for embryonic stem cell differentiation. Genesis, 38, 32-38. PMID: 14755802 Ladd, P.D., Butler, J. S., and Skalnik, D.G. (2004) Identification of a genomic fragment that directs hematopoietic-specific expression of Rac2 and analysis of the DNA methylation profile of the gene locus. Gene, 341, 323-333. PMID: 15474314 Carlone, D.L. Lee, J-H., Young, S.R.L., Dobrota, E., Butler, J.S., Ruiz, J., and Skalnik, D.G. (2005) Reduced cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein. Molecular and Cellular Biology 25, 4881-4891. PMID: 15923607; PMCID: 1140577. Lee, J.-H. and Skalnik, D.G. (2005) CpG binding protein (CXXC finger protein 1) is a component of the mammalian Set1 histone H3-Lys4 methyltransferase complex, the analogue of the yeast Set1/COMPASS complex. Journal of Biological Chemistry 280, 41725-41731. PMID: 16253997

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Balch, C., Yan, P., Craft, T., Young, S., Huang, T. H-M., Skalnik, D.G., and Nephew, K.P. (2005) Antimitogenic and chemosensitizing effects of the methylation inhibitor Zebularine in ovarian cancer. Molecular Cancer Therapeutics 4, 1505-1514. PMID: 16253997 Young, S.R.L., Mumaw, C., Marrs, J.A., and Skalnik, D.G. (2006) Antisense targeting of CXXC finger protein 1 results in reduced cytosine methylation and disruption of hematopoiesis in Zebrafish. Journal of Biological Chemistry 281, 37034-37044. PMID: 17023431 Butler, J.S., Lee, J.H., and Skalnik, D.G. (2006) PAGE separation of hemimethylated or unmethylated oligonucleotide substrates to distinguish between maintenance and de novo DNA methyltransferase activity. Journal of Biochemical and Biophysical Methods 68, 195-199. PMID: 16901546 Lee, J.H., Tate, C., You, J., and Skalnik, D.G. (2007) Identification and characterization of the human Set1B histone H3-Lys4 methyltransferase complex. Journal of Biological Chemistry 282, 13419-13428. PMID: 17355966 Young, S.R.L., and Skalnik, D.G. (2007) CXXC finger protein 1 is required for normal proliferation and differentiation of the PLB-985 myeloid cell line. DNA and Cell Biology 26, 80-90. PMID: 17328666 Lee, J.-H. and Skalnik, D.G. (2008) Wdr82 is a CTD-binding protein that recruits the Set1A histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. Molecular and Cellular Biology 28, 609-618. PMCID: 2223426. Butler, J.S., Lee, J.-H., and Skalnik, D.G. (2008) CFP1 interacts with DNMT1 independently of its association with the Set1 histone H3 K4 methyltransferase complexes. DNA and Cell Biology 27, 533-543. PMCID: 2754740. Nephew, K.P., Balch, C., and Skalnik, D.G. (2008) Methyl group acceptance assay for the determination of global DNA methylation levels. Methods in Molecular Biology 507, 35-41. Butler, J.S., Palam, L.R., Tate, C.M., Sanford, J.R., Wek, R. C., and Skalnik, D.G. (2009) DNMT1 protein synthesis is reduced in CFP1-deficient embryonic stem cells. DNA and Cell Biology 28, 223-231. NIHMS: 157819. Muthukrishnan, R., and Skalnik, D.G. (2009) Identification of a minimal ciselement and cognate trans-factor(s) required for induction of Rac2 gene expression during K562 cell differentiation. Gene 440, 63-72. PMCID: 2697498. Tate, C.M., Lee,J.-H., and Skalnik, D.G. (2009) CXXC finger protein 1 contains redundant functional domains that support embryonic stem cell cytosine methylation, histone methylation, and differentiation. Molecular and Cellular Biology 29, 3817-3831. PMCID: 2704740. Tate, C.M., Fishel, M., Holleran, J., Egorin, M.J., and Skalnik, D.G. (2009) Embyronic stem cells deficient in epigenetic regulator CFP1 demonstrate hypersensitivity to DNA-damaging agents and decreased APE1 protein

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expression and endonuclease activity. DNA Repair 8, 1411-1423. NIHMSID:149634. 49. Tate, C.M., Lee, J.-H., and Skalnik, D.G. (2010) CXXC finger protein 1 restricts the Setd1A histone H3K4 methyltransferase complex to euchromatin. FEBS J. 277, 210-223. NIHMSID: 165117 Lee, J.-H., You, J., Dobrota, E., and Skalnik, D.G. (2010) Identification and characterization of a novel human PP1 phosphatase complex. Journal of Biological Chemistry 285, 24466-24476. Skalnik, D.G., Li, B., Young, S.R.L., Dobrota, E., Lee, J.-H., Tate, C., Haneline, L., HogenEsch, H., and Chun, K.T. The epigenetic regulator CXXC finger protein 1 is essential for definitive hematopoiesis and adult mouse survival. Manuscript in preparation. Srirangam, A., Mitra, R., Milani, M., Kathuria, H., Fukuda, S., Skalnik, D.G., Pelus, L., and Potter, D.A. The HIV protease inhibitor Ritonavir inhibits lung cancer cells, in part, by inhibition of surviving. Submitted to the Journal of Thoracic Oncology.

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Invited Reviews: 1. Skalnik, D.G. (2002) Transcriptional mechanisms regulating myeloid-specific genes. Invited review article (peer reviewed) in Gene, 284, 1-21. PMID: 11891042 2. 3. Skalnik, D.G. (2002) Transcriptional regulation in myeloid cells. Invited review article in Recent Research Developments in Immunology 4, 77-85. Skalnik, D.G. (2004) Methyl-CpG binding proteins, in Encyclopedia of Biological Chemistry (W.J. Lennarz and M.D. Lane, eds), Elsevier, Oxford, Vol. 2, pp 672675. Nephew, K.P., Balch, C., and Skalnik, D.G. (2009) Methyl group acceptance assay for the determination of global DNA methylation levels. In DNA Methylation: Methods and Protocols, Second edition, vol 507. Humana Press. Jorg Tost (ed). Skalnik, D.G. (2010) The epigenetic regulator Cfp1. Biomolecular Concepts. Invited review article (Peer reviewed). In Press. Skalnik, D.G. (2011) Methyl-CpG binding proteins, in Encyclopedia of Biological Chemistry (W.J. Lennarz and M.D. Lane, eds), Elsevier, Oxford, second ed., In Press. Skalnik, D.G. (2010) Genetic and biochemical analyses of Cfp1, an epigenetic regulator of mammalian development. International Drug Discovery, Sept/Oct.

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Abstracts and meeting symposia: 1. Skalnik, D.G., Dorfman, D.M., Perkins, A.S., Jenkins, N.A., Copeland, N.G., and Orkin, S.H. (1990) Targeting of transgene expression to monocytes by cytochrome b heavy chain 5'-flanking sequences induces histiocytic malignancies. Transaction of the Association of American Physicians 103, 129136. This abstract was presented orally by D.G.S. at the plenary session of the joint American Federation for Clinical Research/Association of American Physicians meeting, Washington, D.C., 1990. 2. Skalnik, D.G., Dorfman, D.M., Williams, D.A., and Orkin, S.H. (1990) An animal model for neuroblastoma. American Federation for Clinical Research/ Association of American Physicians/AFCR, Washington, D.C. This abstract was presented orally by D.G.S. at a mini-symposium during the conference. Hopkins, P.J., Skalnik, D.G., Eklund, E.A., Newburger, P.E., and Curnutte, J.T. (1992) Mutations in the gp91-phox gene promoter region result in clonal expression of cytochrome b558 and symptomatic chronic granulomatous disease. Blood 80, 251a. Presented orally by P.J.H. at the American Society of Hematology meeting, Anaheim, CA, December, 1992. Luo, W., and Skalnik, D.G. Transcriptional regulation of the gp91-phox gene. Presented by Wen Luo at the Federation of American Societies for Experimental Biology meeting, Washington, D.C., May 27, 1994 Eklund, E.A., Lee, S.-W., and Skalnik, D.G. Cloning of a human protein that exhibits DNA-binding activity and is similar to ribosomal protein S1. Blood 84, 132A. American Society of Hematology meeting, Dec. 1994, Nashville, TN. Eklund, E.A., Luo, W., and Skalnik, D.G. The gp91-phox promoter is under both positive and negative regulation. Phagocyte Gordon Conference, June 11-16, 1995, Plymouth, NH. Presented by D.G.S. as both a poster and a short oral presentation. Hawkins, S., Theobald, L., and Skalnik, D.G. Chemical induction of a differentiated phenotype of prostatic neuroendocrine cell lines derived from a transgenic mouse tumor model. M.D./Ph.D. student conference. July 15-17, 1995, Aspen, CO. Ou, X., Pollock, J., Dinauer, M., Lee, S.-W., Gharehbaghi-Schnell, E., and Skalnik, D. G. (1995) Identification and functional characterization of the murine Rac2 promoter. Blood 86, 28a. Presented as a poster at the American Society of Hematology meeting, Seattle, WA. Catt, D.M., Luo, W., and Skalnik, D.G. Study of the novel DNA-binding domains of CCAAT displacement protein, a repressor of the gp91-phox promoter. (1996) Presented to the American Society of Hematology meeting, Orlando, FL, December, 1996. Voo, K.S., and Skalnik, D.G. Molecular cloning of HAF-1, a novel transcription factor required for gp91-phox expression. (1996) Presented to the American Society of Hematology meeting, Orlando, FL, December, 1996.

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Jacobsen, B.M., Schnell, E., and Skalnik, D. G. Positive cis-acting elements in the proximal gp91-phox promoter. (1997) Presented to the American Society of Hematology meeting, San Diego, CA, December, 1997. Voo, K.S., Carlone, D.L., and Skalnik, D.G. Characterization of HAF-1, a novel transcription factor required for myeloid-specific expression of the gp91-phox gene. (1997) Presented to the American Society of Hematology meeting, San Diego, CA, December, 1997. Teerawatanusuk, N., Skalnik, D.G., and Carr, L.G. CCAAT displacement protein (CDP) negatively regulates human tryptophan hydroxlase gene. (1998) Presented to the Experimental Biology meeting, Carlone, D.L., Rothrock, J.K., Pescovitz, O.H., and Skalnik, D.G. (1998) Expression of a novel DNA-binding protein, HAF-1, in male germ cells. Presented to the Endocrine Society, New Orleans, June, 1998. Hawkins, S., and Skalnik, D.G. (1998) Differential binding of a tissue specific nuclear matrix protein, SATB1, to the proximal gp91-phox promoter during myeloid cell differentiation. M.D./Ph.D. student conference, July, Aspen, CO, Voo, K.S., and Skalnik, D.G. (1998) PU.1 and Elf-1 induce expression of gp91phox via a promoter element mutated in a subset of chronic granulomatous disease patients. Presented to the American Society of Hematology meeting, Miami, FL, December, 1998. Hawkins, S., Man, W., Kohwi-Shigematsu, T., and Skalnik, D. G. (1998) Differential binding of the nuclear matrix protein SATB1 to the gp91-phox promoter during myeloid cell differentiation. Presented to the American Society of Hematology, Miami, FL, December, 1998. Voo, K.S., Carlone, D. L., Jacobsen, B.M., Flodin, A., and Skalnik, D.G.(1999) Identification and characterization of a novel mammalian CpG binding transcriptional activator that shares a motif with DNA methyltransferase and HRX proteins. Abstract submitted for presentation at the annual international meeting of the International Society of Experimental Hematology (ISEH) Monaco. Collado, A., Skalnik, D., and Taylor, M. (1999) Possible involvement of novel elements in the IFN-g induction of INDO in ME180 and HEPG2 cell lines. Skalnik, D. and Jacobsen, B. YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91-phox promoter. Poster presented at American Society of Hematology 1999, New Orleans. Hawkins, S., and Skalnik, D.G. The gp91-phox promoter contains numerous binding sites for the nuclear matrix protein SATB1 and associates with the nuclear matrix during myeloid cell differentiation. Poster presented at American Society of Hematology 1999, New Orleans. Voo, K., Carlone, D., Jacobsen, B., Flodin, A., and Skalnik, D.G. Cloning of a mammalian transcriptional activator that binds unmethylated CpG motifs and

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shares a CXXC domain with DNA methyltransferase, human trithorax, and methyl-CpG binding domain protein. Poster presented at American Society of Hematology 1999, New Orleans. 23. Skalnik, D.G., and Voo, K.S. Characterization of the DNA-binding domain of CpG binding protein. Poster presented at American Society of Hematology 2000, San Francisco. Carlone, D.L., Lee, J.-H., Voo, K.S., Ruiz, J., and Skalnik, D.G. (2001) Analysis of the DNA-binding properties and in vivo function of CpG binding protein. AACR special conference Cancer and Chromosomal Organization/Epigenetics of Cancer. Palm Desert, CA, Oct 17-21. Selected also for oral presentation (top 10% of abstracts). Carlone, D.L., Ladd, P.D., Ruiz, J., and Skalnik, D.G. (2001) CpG binding proteinis crucial for early embryonic development. Keystone Symposium, Mechanisms of Eukaryotic Transcriptional Regulation.. Santa Fe, NM Feb. 26March 4. Lee, J.H., and Skalnik, D.G. (2002) CpG binding protein is a transcriptional activator that is enriched in active euchromatin regions and is associated with the nuclear matrix. Keystone Symposium - Chromatin structure and function, Jan. 26-31, 2002, Santa Fe, New Mexico. Ladd, P.D., and Skalnik, D.G. (2002) Identification and characterization of Rac2 genomic elements that direct hematopoietic cell-specific expression. Poster presented at the American Society of Cell Biology, December 14-18, San Francisco, CA. Carlone, D.L., Lee, J.H., and Skalnik, D.G. (2003) Defects in cytosine methylation and histone modification in embryonic stem cells lacking CpG binding protein. Presented at the Epigenetics and Cancer Gordon Conference, Jan 5-10, Ventura, CA. Carlone, D.L., Lee, J.H., and Skalnik, D.G. (2003) Defects in cytosine methylation and histone modification in embryonic stem cells lacking CpG binding protein. Presented at the Chromatin Keystone Symposium held Jan 1015, Big Sky, Montana. Chosen for oral presentation (top 10% of abstracts). Lee, J.H., Hart, S.R.L., and Skalnik, D.G. (2004) Histone deacetylase activity is required for embryonic stem cell differentiation. Presented as a poster at the Epigenetic Regulation Keystone Conference, January 21-26, Lake Tahoe, CA. Lee, J.H., Dobrota, E., and Skalnik, D.G. (2004) Decreased cytosine methylation and altered histone modifications in embryonic stem cells lacking CpG binding protein, a component of the set1 histone H3-Lys4 methyltransferase. AACR epigenetics and cancer symposium. Kona, Hawaii, Nov 10-14. Young, S., and Skalnik, D.G. (2004) CGBP is required for zebrafish hematopoiesis. Poster presented at the national meeting of the American Society of Hematology, San Diego, Dec. 3-7.

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Butler, J.S. and Skalnik, D.G. (2005) Role of the DNA methyltransferases in the CGBP-null phenotype. Poster presented at the Cancer Genetics and Epigenetics Gordon Conference, Ventura, CA, Jan 23-28. Lee, J.-H., Dobrota, E., and Skalnik, D.G. (2005) Decreased cytosine methylation and altered histone modifications in embryonic stem cells lacking CpG binding protein. Poster presented at the Cancer Genetics and Epigenetics Gordon Conference, Ventura, CA, Jan 23-28. Butler, J., and Skalnik, D.G. (2006) CXXC-finger protein 1 regulates DNA methyltransferase protein 1 expression and activity. Selected for oral presentation at the Midwest meeting of the Society for Pediatric Research, October 19-20, Indianapolis, IN. Butler, J., and Skalnik, D.G. (2006) CXXC-finger protein 1 regulates DNA methyltransferase protein 1 expression and activity. Poster presentation at the Cancer Biology Training Program Retreat. Asilomar, CA. Oct 28-30, 2006. Muthukrishnan, R., and Skalnik, D.G. (2006) Regulation of human Rac2 during human chronic myelogenous leukemia cell line (K562) differentiation. Poster presented a the American Society for Cell Biology meeting, December 9-13, San Diego, CA. Lee, J.-H., Tate, C.M., Youi, J.-S., and Skalnik, D.G. (2006) Characterization of the human Set1A and Set1B histone H3-Lys4 methyltransferase complexes. Selected for oral presentation. ASBMB symposium: Transcriptional regulation by chromatin and RNA polymerase II. November 2-5, Kiawah Island, S.C. Butler, J., and Skalnik, D.G. (2006) CXXC-finger protein 1 regulates DNA methyltransferase protein 1 expression and activity. Poster presentation at the Abcam Chromatin and Epigenetics conference, December 3-8, Dominican Republic. Courtney M. Tate, Jeong-Heon Lee, and David G. Skalnik (2007) CXXC Finger Protein 1 DNA Binding Activity or Association with the Set1 Complex are Important for Embryonic Stem Cell Differentiation and Appropriate Cytosine Methylation. Poster presentation at the Keystone Symposium on Chromatin, Breckenridge, CO, April 11-16. Tate, C.M. and Skalnik, D.G. (2007) CXXC finger protein 1 DNA binding activity or association with the Set1 complexes are important for embryonic stem cell differentiation and cytosine methylation. Poster presented at the Midwest society for pediatric research meeting, Indianapolis, IN. Muthukrishnan, R., and Skalnik, D.G. (2007) Regulation of human Rac2 during human chronic myelogenous leukemia cell line (K562) differentiation. Oral presentation at the Midwest society for pediatric research meeting, Indianapolis, IN.

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Muthukrishnan, R., and Skalnik, D.G. (2007) Regulation of human Rac2 during human chronic myelogenous leukemia cell line (K562) differentiation. Poster presentation at the American Society of Cell Biology, Washington DC, December, 2007. Lee, J.-H., Tate, C.M., Dobrota, E., Li, B., Young, S.R.L., Chun, K.T., and Skalnik, D. G. (2009) CXXC finger protein 1: SETting the stage for epigenetic regulation. Midwest symposium; Role of Epigenetics in Cancer: Mechanisms and Therapy. Ohio State University. Nov. 9. Invited speaker. Skalnik, D.G., Lee, J.-H., Tate, C.M., Drobrota, E., Li, B., Young, S.R.L., and Chun, K. T. (2010) FASEB Summer Research Conferences. Biological methylation: form DNA to histones and beyond. Genetic and biochemical analyses of CXXC finger protein 1, an epigenetic regulator of mammalian development. June 6-11, 2010, Carefree Resort, AZ. Invited speaker. Clouaire, T., Webb, S., Andrews, R., Auger, K., Skalnk, D.G., and Bird, A. (2010) Role of the CpG binding protein Cfp1 in mouse ES cells. EMBL epigenetics conference, Europe.

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CURRICULUM VITAE
DAVID L. STOCUM Office Address Department of Biology Indiana University-Purdue University 723 W. Michigan St. Indianapolis, IN 46202 Telephone: (317) 274-0627 Fax: (317) 274-2846 Email: dstocum@iupui.edu Home Address 611 N. Park St. Indianapolis, IN 46204 Telephone: (317) 636-8523

PERSONAL HISTORY
CITIZENSHIP U.S.A. ACADEMIC DEGREES B.A. M.S. Ph.D. 1961 1963 1968 Susquehanna University University of Illinois University of Pennsylvania (Biology and Psychology) (Zoology) (Cell and Developmental Biology)

ACADEMIC APPOINTMENTS University of Illinois, Urbana-Champaign Assistant Professor Department of Zoology Associate Professor Department of Genetics and Development Director, Honors Biology Program Professor Departments of Genetics and Development, and Anatomical Sciences Acting Head Department of Anatomical Sciences Professor College of Medicine Professor Department of Cell and Structural Biology 1968-1973

1973-1981

1974-1976 1981-1987

1984-1986

1984-1990

1987-1990

Indiana University -Purdue University Indianapolis Dean and Professor of Biology, School of Science Professor of Biology and Dean Emeritus Director, Indiana University Center for Regenerative Biology and Medicine July 1,1989-July 31, 2004

Aug 1, 2004-present

October 1, 2005-present

SCHOLARLY ACTIVITIES AND ACCOMPLISHMENTS

RESEARCH AND TEACHING INTERESTS Cell and Developmental Biology Regenerative Biology and Medicine Mechanisms of Regeneration Cell Transplantation Bioartificial Tissues Stimulation of Regeneration in vivo

MEMBERSHIP IN PROFESSIONAL AND CIVIC ORGANIZATIONS Council of Colleges of Arts and Sciences (1990-2004) American Association for the Advancement of Science American Association of Anatomists Federation of American Societies for Experimental Biology Society for Developmental Biology Wound Healing Society American Conference of Academic Deans (1990-2004) Rotary Club of Indianapolis (1995-2004) Literary Club of Indianapolis

HONORS AND AWARDS NIH Fellow in Developmental Biology, 1965-1968

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Associate in the Center for Advanced Study, University of Illinois, 1986-1990 Elected Fellow, American Association for Advancement of Science, 1973 Recipient, Singer Medallion For Regeneration Research, 1988 CONSULTANCIES Research consultant to Eli Lilly and Company on muscle development, April 15, 1996 April 14, 1997. Research consultant to Professor Hiroyuki Ide, Tohoku University, Japan, for a project entitled Retinoic Acid and Pattern Formation in Limb Regeneration, 1995-1997. Research Advisor, Yoshizato MorphoMatrix Project, Japan Science and Technology Corporation (formerly Research Development Corporation of Japan), April 1, 1996 - September 30, 1997. Cook Biotech, West Lafayette, IN, January 1999-present PUBLICATIONS ( *Peer Reviewed)

*Stocum, D.L. 1966 The effects of thiourea and cortisone acetate on duodenal length and diameter and on morphogenesis of duodenal villi in chick embryos. J. Morph. 118:183-196. *Stocum, D.L. 1968a The urodele limb regeneration blastema: A self-organizing system. I. Differentiation in vitro. Dev. Biol. 18:441-456. *Stocum, D.L. 1968b The urodele limb regeneration blastema: A self-organizing system. II. Morphogenesis and differentiation of autografted whole and fractional blastemas. Dev. Biol. 18:457-480. *Morzlock, F.V. and D.L. Stocum 1971 Patterns of RNA synthesis in regenerating limbs of the adult newt, Triturus viridescens. Dev. Biol. 24:106-118. *Morzlock, F.V. and D.L. Stocum 1972 Neural control of RNA synthesis in regenerating limbs of the adult newt, Triturus viridescens. Roux's Archiv Dev. Biol. 171:170-180. *Montalvo, F., H.E. Conrad, and D.L. Stocum 1972 Relative rates of protein synthesis and reaggregation in embryonic chick liver cells cultured in defined and supplemented media. Exp. Cell Res. 74:509-513. *Stocum, D.L. and G.E. Dearlove 1972 Epidermal-mesodermal interaction during morphogenesis of the limb regeneration blastema in larval salamanders. J. exp. Zool. 181:49-62. *Dearlove, G.E. and D.L. Stocum 1974 Denervation-induced changes in soluble protein content during forelimb regeneration in the adult newt, Notophthalmus viridescens. J exp. Zool. 190:317-328. *Stocum, D.L. 1975a Outgrowth and pattern formation during limb ontogeny and regeneration. Differentiation 3:167-182. *Stocum, D.L. 1975b Regulation after proximal or distal transposition of limb regeneration blastemas and determination of the proximal boundary of the regenerate. Dev. Biol. 45:112-136.

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Stocum, D.L. 1977 Tissue interactions in limb regeneration. In: Vertebrate Limb and Somite Morphogenesis. D.A. Ede, J.R. Hinchliffe, and M. Balls, eds. Cambridge University Press, Cambridge, pp. 347-372. *Stocum, D.L and D.A. Melton 1977 Self-organizational capacity of distally transplanted limb regeneration blastemas in larval salamanders. J. exp. Zool. 201:451-462. *Stocum, D.L. 1978a Regeneration of symmetrical hindlimbs in larval salamanders. Science 200:790-793. Stocum, D.L. 1978b Organization of the morphogenetic field in regenerating amphibian limbs. Am. Zool. 18:883-896. *Stocum, D.L. 1979 Stages of forelimb regeneration in Ambystoma maculatum. J. exp. Zool. 209:395-416. *Stocum, D.L., R.M. Davis, M. Leger, and H.E. Conrad 1979 Development of the tibiotarsus in the chick embryo: Biosynthetic activities of histologically distinct regions. J. Embryol. exp. Morph. 54:155-170. *Stocum, D.L. 1980a Patterns of mitotic index, cell density and growth in regenerating Ambystoma maculatum limbs. J. exp. Zool. 212:233-242. *Stocum, D.L. 1980b Autonomous development of reciprocally exchanged regeneration blastemas of normal forelimbs and symmetrical hindlimbs. J. exp. Zool. 212:361-371. *Stocum, D.L. 1980c Intercalary regeneration of symmetrical thighs in the axolotl, Ambystoma mexicanum. Dev. Biol. 79: 276-295. *Pescitelli, M.J. and D.L. Stocum 1980 The origin of skeletal structures during intercalary regeneration of larval Ambystoma limbs. Dev. Biol. 79:255-275. *Pescitelli, M.J. and D.L. Stocum 1981 Non-segmental organization of positional information in regenerating Ambystoma limbs. Dev. Biol. 82:69-85. *Stocum, D.L. 1981 Distal transformation in regenerating double anterior axolotl limbs. J. Embryol. exp. Morph. 65:3-18 (supplement). *Stocum, D.L. 1982 Determination of axial polarity in the urodele limb regeneration blastema. J. Embryol. exp. Morph. 71:193-214. *Stocum, D.L and J.F. Fallon 1982 Control of pattern formation in urodele limb ontogeny: A review and a hypothesis. J. Embryol. exp. Morph. 69:7-36. Stocum, D.L. 1983a Distal transformation in regenerating urodele limbs. In: Limb Development and Regeneration. A. Caplan, J. Fallon, R. Kelley, P. Goetinck, and J. MacCabe, eds. Alan Liss Pub., New York, pp. 468-476. Stocum, D.L. 1983b Morphogenesis of the amphibian limb regeneration blastema. In: Nerve, Organ, and Tissue Regeneration: Research Perspectives. F.J. Seil, ed. Academic Press, New York, pp. 377-407.

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*Nardi, J.B. and D.L. Stocum 1983 Surface properties of regenerating limb cells; Evidence for gradation along the proximodistal axis. Differentiation 25:27-31. Stocum, D.L and J.F. Fallon 1984 Urodele limb development. In: Pattern Formation: Primer in Developmental Biology. G. Malacinski and S.V. Bryant, eds. MacMillian Pub. Co., New York, pp. 507-520. *Stocum, D.L. 1984 The urodele limb regeneration blastema: Determination and organization of the morphogenetic field. Differentiation 27:13-28. Stocum, D.L. 1984 Animal Morphogenesis. Encyclopedia of Science and Technology, Macmillan Co. Technology, Macmillan Co. *Thoms, S.D. and D.L. Stocum 1984 Retinoic acid-induced pattern duplication in regenerating urodele limbs. Dev. Biol. 103:319-328. *Stocum, D.L. and S.D. Thoms 1984 Retinoic acid-induced pattern completion in regenerating double anterior limbs of urodeles. J. exp. Zool. 232:207-215. Stocum, D.L. 1985 The role of the skin in urodele limb regeneration. In: Control of Forelimb Regeneration. R. Sicard, ed. Oxford University Press, pp. 32-53. *Niazi, I.A., M.J. Pescitelli, and D.L. Stocum 1985 Stage-dependent effects of retinoic acid on urodele limb regeneration. Roux's Archiv Dev. Biol. 194:355 363. Stocum, D.L. 1986. Retinoids: Probes for understanding pattern regulation in regenerating amphibian limbs. In: The Developmental Implications of Intercellular Communication. R.M. Gorczynski, ed. Academic Press, New York, pp. 165-181. *Stocum, D.L. 1986 Morphogenesis of the Embryonic Limb. Encyclopedia of Science and Technology, Macmillan Co. *Kim, W. -S. and D.L. Stocum. 1986a Retinoic acid modifies positional memory in the anteroposterior axis of regenerating axolotl limbs. Dev. Biol. 114:170-179. *Kim, W. -S. and D.L. Stocum 1986b Effects of retinoic acid on regenerating normal and double half limbs of axolotls: Histological studies. Roux's Archiv Dev. Biol. 195:243-251. *Kim, W.-S and D.L. Stocum 1986c Retinoid-induced pattern duplication in regenerating limbs: Effects of synthetic retinoids and different amputation levels. Roux's Archiv Dev. Biol. 195:243251. *Slobin, J.A., D.L. Stocum, and W.D. O'Brien, Jr. 1986 Amphibian limb regeneration curves generated by the scanning laser acoustic microscope. J. Histochem. Cytochem. 34:53-56. *Stocum, D.L. and K. Crawford 1987 Use of retinoids to analyze the cellular basis of positional memory in regenerating axolotl limbs. Biochem. Cell Biol. 65:750761. Stocum, D.L. 1988 Modification of positional memory in regenerating limbs by retinoic acid. In: Regeneration and Development. S. Inoue, T. Shirai, M. Egar, S. Aiyama, J. Geraudie, T. Nobunaga and N.L. Sato, eds. Okada Pub. Co., Maebashi.

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*McCormick, A.M., H.E. Shubeita, and D. L. Stocum 1988 Cellular retinoic acid binding protein: Detection and quantitation in regenerating axolotl limbs. J. Exp. Zool. 245:270-276. *Crawford, K. and D.L. Stocum 1988a Retinoic acid coordinately proximalizes regenerate pattern and blastema differential affinity in axolotl limbs. Development 102:687-698. *Crawford, K. and D.L. Stocum. 1988b Retinoic acid proximalizes level-specific properties responsible for intercalary regeneration axolotl limbs. Development 104:703-712. Stocum, D.L. 1989 The use of retinoids to explore the cellular and molecular basis of positional memory in regenerating amphibian limbs. In: Recent Trends in Regeneration Research. V. Kiortsis, S., Koussoulakis and H. Wallace, eds. Plenum Press, New York, pp. 295-312. *Stocum, D.L. and V.I. Mitashov 1990 Pattern regulation in regenerating limbs. Ontogenez 27:5-31 (In Russian). English translation by Plenum Publishing Corp. *Ludolph, D.C., J.A. Cameron and D. L. Stocum 1990 The effect of retinoic acid on positional memory in the dorsoventral axis of regenerating axolotl limbs. Dev. Biol. 140:41-52. Stocum, D.L. and T.L. Karr 1990 (eds) The Cellular and Molecular Biology of Pattern Formation. Oxford University Press, New York. Stocum, D.L. and M. Maden 1990 Regenerating limbs. Methods in Enzymology 190:189-201. Stocum, D.L. 1991a Retinoic acid and pattern formation in regenerating limbs. Seminars in Dev. Biol. 2:199-210. *Stocum, D.L. 1991b Limb regeneration: a call to arms (and legs). Cell 67:5-8. Stocum, D.L. 1992 Dynamics of the gastrulation movements and determination of the mesoderm in amphibian embryos. In: Morphogenesis: An Analysis of the Development of Biological Structures. E.F. Rossomando and S. Alexander, eds. Marcel Dekker, Inc., New York, pp. 319-386. *Monkemeyer, J., D.C. Ludolph, J.A. Cameron and D.L. Stocum 1992 Retinoic acid-induced change in anteroposterior positional identity in regenerating axolotl limbs is dose-dependent. Dev. Dynamics 193:286-294. Ludolph, D.C., J.A. Cameron, and D.L. Stocum 1993 Asymmetric effects of retinoic acid on pattern formation in the transverse axes of regenerating axolotl limbs: Test of a hypothesis. In: Limb Development and Regeneration, J.F. Fallon, P.F. Goetinck, R.O. Kelley, and D.L. Stocum, eds. Wiley-Liss, Inc., New York, pp. 739-747. *Ludolph, D.C., J.A. Cameron, A.W. Neff, and D.L. Stocum 1993 Cloning and tissue specific expression of the axolotl cellular retinoic acid binding protein. Dev. Growth and Diff. 35: 341-347. *Ludolph, D.C., J.A. Cameron, and D.L. Stocum 1993 Test of a model for the effects of retinoic acid on urodele limb regeneration. Dev. Dynamics 198: 77-85. Stocum, D. L. 1995 Wound Repair, Regeneration and Artificial Tissues. Austin, TX; R. G. Landes Co.

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*Chernoff, E.A.G. and D.L. Stocum 1996 Developmental aspects of spinal cord and limb regeneration in urodeles. Dev. Growth and Diff. 37: 133-147. *Stocum, D.L. 1996 A conceptual framework for analyzing axial patterning in regenerating urodele limbs. Int. J. Dev. Biol. 40: (773-783). *Stocum, D.L. 1996 Tissue restoration: approaches and prospects. Wound Repair and Regeneration 4: 3-15. Stocum, D.L. 1997 New Tissues from Old. Science 276: 15 (Editorial). *Stocum, D.L. and P. M. Rooney 1997 Responding to resource constraints: a departmentally based system of responsibility center management. Change 29 (#5): 50-57. Stocum, D.L. 1998 Bridging the Gap: Restoration of Structure and Function in Humans. In: Cellular and Molecular Basis of Regeneration: From Invertebrates to Humans. P. Ferretti and J. Geraudie, eds., John Wiley and Sons, Ltd., pp.411-490. Stocum, D.L. 1998 Regenerative biology and engineering: strategies for tissue restoration. Wound Repair and Reg. 6: 276-290. *Stocum, D.L. 1998 New tissues for old. J. Minn. Acad. Sci. 63: 23-30. Stocum, D.L. 1999 Regenerative biology: a millennial revolution. Sem. Cell & Dev Biol 10: 433-440. Stocum, D.L. 1999 Limb regeneration: re-entering the cell cycle. Curr Biol 9: R644-R646. *Stocum, D.L. 1999 Regeneration of Vertebrate Tissues: Model Systems. Encyclopedia of Life Sciences, MacMillan Reference Ltd. Stocum, D.L. 2000 Transplantation and culture techniques for the analysis of urodele limb regeneration. In: Developmental Biology Protocols, Volume I, R.S. Tuan and C.W. Lo, eds, Humana Press Inc., Towota, NJ, pp. 67-74. *Stocum, D.L. 2000 Regeneration of the amphibian limb. Encyclopedia of Life Sciences, MacMillan Reference Ltd. *Stocum, D.L. 2000 Regenerative biology and medicine in the 21st century. Reg Med 1: 17-20. E-biomed, www.liebertpub.com/reg Stocum, D.L. 2000 Frog limb deformities: an eco-devo riddle wrapped in multiple hypotheses surrounded by insufficient data. Teratology 62:147-150. Stocum D. L. 2000 Urban universities: a model for the 21st century. Vital Speeches of the Day LXVI, #23: 715-718. *Picciolo GL and Stocum DL 2001 ASTM lights the way for tissue engineered medical products standards. ASTM Standardization News 29 (#1):29-35 Stocum D.L. 2001 Regeneration of the spinal cord. The World and I F ebruary: 132-139.

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*Stocum D. L. 2001 Regenerative biology and medicine: a new era in bioengineering. Cardiac and Vasc Reg 1:157-169. Stocum D. L. 2001 The evolution of 21st Century public higher education: the urban university as prototype. Metropolitan Universities Journal 12:10-19. Stocum DL 2001 Rx for tissue restoration: regenerative biology and medicine. Korean J Biol Sci 5: 91-99. *Loeffler IK, Stocum DL, Fallon JF, and Meteyer CU 2001 Leaping lopsided: a review of the current hypotheses regarding etiologies of limb malformations in frogs. Anat Rec (New Anat) 265:228-245. Stocum DL 2002 Stem cells in regenerative biology and medicine. Wound Rep Reg 94:29-42. Stocum DL 2002 Regenerative biology and medicine. J Musculoskel Neuron Interact 2 (3):1-4. Stocum DL 2002 Regenerative biology and medicine. J Musculoskel Neuron Interact 2 (4):383-384. Stocum DL 2002 A tail of transdifferentiation. Science 298:1901-1902. *Nye HLD, Cameron JA, Chernoff EG, Stocum DL 2003 Regeneration of the urodele limb: a review. Dev Dynam 226:280-294. *Stocum DL 2003 The regenerative biology of amphibians: gateway to a regenerative medicine. J Regen Med 3:1-6. *Nye HLD, Cameron JA, Chernoff EAG, Stocum DL 2003 Extending the table of stages of normal development of the axolotl: limb development. Dev Dynam 226:555-560. *Chernoff EAG, Stocum DL, Nye HLD, Cameron JA 2003 Urodele spinal cord regeneration and related processes. Dev Dynam 226:295-307. *King MW, Nguyen T, Calley J, Harty MW, Muzinich MC, Mescher AL, Chalfant C, NCho M, McLeaster K, McEntyre J, Stocum DL, Smith RC, Neff AW (2003) Identification of genes expressed during Xenopus laevis limb regeneration by using subtractive hybridization. Dev Dynamics 226:398-409.

*Stocum DL, Nye HLD, Cameron JA, Chernoff EAG 2003 What we know about urodele
(especially axolotl) early development: a review of gamete formation and fertilization. Axolotl Newsletter 30 (Summer): 4-11. Stocum DL 2003 Stem cells in amphibian regeneration. In: Stem Cell Handbook. Sell S, ed. Humana Press, Towota, NJ, pp 89-100. Stocum DL 2004 Amphibian regeneration and stem cells. In Regeneration: Beyond the Stem Cells. Heber-Katz E (ed). Springer Verlag, Heidelberg, pp 1-70. *Stocum DL, Nye HLD, Cameron JA, Chernoff EAG 2004 What we know about urodele (expecially axolotl) early development: a review of cleavage, gastrulation, and organogenesis. Axolotl Newsletter 30:4-10.

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Stocum DL 2004 Tissue Restoration Through Regenerative Biology and Medicine. Adv in Anat, Embryol and Cell Biol 176:1-104. Stocum DL 2004 Regenerative Biology and Medicine: An Overview. Cell Sci 1:1-14. Stocum DL 2005 Stem cells in CNS and cardiac regeneration. In: Advances in Biochemical Engineering/Biotechnology: Regenerative Medicine. Yannas IV (ed). Springer-Verlag, pp 135159. *Stocum DL 2005 Regeneration of Vertebrate Tissues: Model Systems. Encyclopedia of Life Sciences, MacMillan Reference Ltd (Revision of previous article) *Stocum DL 2005 Regeneration of the amphibian limb. Encyclopedia of Life Sciences, MacMillan Reference Ltd, (Revision of previous article) Stocum DL 2005 Morphogenesis. McGraw-Hill Encyclopedia of Science and Technology (Revision of previous article) Stocum DL 2006 Regenerative Biology and Medicine. San Diego, Academic Press/Elsevier, 465 pp. Stocum DL 2007 Acceptable nAGging. Science 318:754-755. Stocum DL 2008 Developmental mechanisms of regeneration and strategies of regenerative medicine. In: Principles of Regenerative Medicine, Atala A, Lanza R, Thompson J, Nerem R, eds. Academic Press/Elsevier, San Diego and New York, pp 100-125. *Palakal M, Sebastian T, Stocum DL 2008 Discovering implicit protein-protein interactions in the cell cycle using bioinformatics approaches. J Biomed Sci 15:317-331. Stocum DL 2008 The Realms of Science: Past, Present, and Future. Indiana Insight, Spring 2008. *Stocum DL, Zupanc GKH 2008 Strtetching the limits: Stem cells in regeneration science. Dev Dynam 237: 3646-3671. *Stocum DL 2009 Regeneration of Vertebrate Tissues: Model Systems. Encyclopedia of Life Sciences, MacMillan Reference Ltd (Revision of previous article) *Stocum DL 2009 Regeneration of the amphibian limb. Encyclopedia of Life Sciences, MacMillan Reference Ltd (Revision of previous article) *Stocum DL 2009 Regenerative Medicine. Encyclopedia Brittanica *Rao N, Jhamb D, Milner DJ, Li B, Song F, Wang M, Voss SR, Palakal M, King MW, Saranjami B, Nye HLD, Cameron JA, Stocum DL 2009 Proteomic analysis of blastema formation in regenerating axolotl limbs. BMC Biology 7:83 Hiles M, Stocum DL 2010 Dermis and non-dermis biologic grafts. General Surgery News, September.

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*Song F, Li B, Stocum DL 2010 Amphibians as research models for regenerative medicine. Organogenesis 6:141-150. *Feng L, Milner DJ, Xia C, Nye HLD, Redwood P, Cameron JA, Stocum DL, Fang N, Jasiuk I 2010 Long bone critical size defect repair by regeneration in adult Xenops laevis hindlimbs. Tiss Eng Part A, in press *Santosh N, Windsor LJ, Mahmoudi BS, Li B, Zhang W, Chernoff EA, Rao N, Stocum DL, Song F 2010 Matrix metalloproteinase expression during blastema formation in regenerationcompetent versus regeneration-deficient amphibian limbs. Dev Dynam, in press. Stocum DL, Rao N 2010 Mechanisms of blastema formation in regenerating amphibian limbs. nd In: Principles of Regenerative Medicine, 2 ed. Atala A, Lanza R, Thompson J, Nerem R, eds, in press *Jhamb D, Rao N, Milner DJ, Song F, Cameron JA, Stocum DL, Palakal MJ 2010 Network based transcription factor analysis of regenerating axolotl limbs. BMC Bioinformatics, revision submitted. *Nye HLD, Milner DJ, Miller P, Miller P, Redwood P, Li B, Stocum DL, Cameron JA 2010 Enhancement of blastema formation and limb regeneration in late-stage Xenopus laevis tadpoles. Wound Rep Reg, submitted, under revision. *Stocum DL, Cameron JA 2010 Looking proximally and distally: 100 years of Limb regeneration and beyond. Dev Dynam, submitted.

ABSTRACTS AND MEETING PRESENTATIONS Stocum, D.L. 1964 Effects of thiourea and cortisone acetate on morphogenesis of previllous ridges and villi in chick embryos. Anat. Rec. 148:340 (American Association of Anatomists, Denver, CO). Stocum, D.L. 1967 In vitro culture of larval urodele limb regenerates. Am. Zool. 7:753. (American Society of Zoologists, New York, NY). Dearlove, G.E. and D.L. Stocum 1974 Denervation-induced changes in soluble protein content of regenerating forelimbs of the adult newt, Notophthalmus viridescens. Am. Zool. 14:1262. (American Society of Zoologists, Phoenix, AZ). Stocum, D.L. 1979 Intercalary regeneration of double anterior limbs. Second Biennial Forum on Regeneration, Ann Arbor, MI. Stocum, D.L. 1980 Intercalary regeneration of double half-anterior thighs in the axolotl, Ambvstoma mexicanum. Anat. Rec. 196:181-182A. (American Association of Anatomists, Omaha, NE). Stocum, D.L. 1980 Intercalary regeneration of symmetrical thighs induced by symmetrical wrist blastemas. Am. Zool. 20-804. (American Society of Zoologists, Seattle, WA). Stocum, D.L. 1981 Determination of polarity in the urodele regeneration blastema. Am. Zool. 21:999. (American Society of Zoologists, Dallas, TX).

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Thoms, S.D. and D.L. Stocum 1983 Effects of retinoids on regenerating urodele limbs. (Fourth Biennial Regeneration Forum, Urbana, IL). Stocum, D.L., S.D. Thoms, and W.-S. Kim 1984 Effects of retinoids on positional information in regenerating limbs of the adult newt, Notophthalmus viridescens. Anat. Rec. 200:174A. (American Association of Anatomists, Seattle, WA). Kim, W.-S. and D.L. Stocum 1984 Retinoids posteriorize positional value in the AP axis of regenerating axolotl limbs. As. Zool. 24:126A. (American Society of Zoologist, Denver, CO). Klein, K.C. and D.L. Stocum 1984 Retinoic acid modifies level specific cell recognition and affinity in regenerating axolotl limbs. Am. Zool. 24:126A. (American Society of Zoologists, Denver, CO). Pescitelli, M.J. and D.L. Stocum 1985 Dolichol has no effect on axolotl limb regeneration. (Fifth Regeneration Forum, Michigan State University, East Lansing, MI). Kim, W.-S. and D.L. Stocum 1985 Effects of retinoic acid on AP axial pattern in regenerating axolotl limbs. (Fifth Regeneration Forum, East Lansing, MI). Crawford, K. and D.L. Stocum 1985 Retinoic acid modifies level-specific positional memory in regenerating axolotl limbs. (Fifth Biennial Regeneration Forum, East Lansing, MI). Stocum, D.L. and M.J. Pescitelli 1985 Effects of retinoic acid and dolichol on urodele limb regeneration. (International Society for Developmental Biology, Los Angeles, CA). Kim, W.-S. and D.L. Stocum 1986 Regeneration of retinoid-treated normal and double half axolotl limbs: Histological studies. Anat. Rec. (American Association of Anatomists, Reno, NV). Crawford, K. and D.L. Stocum 1986 Retinoic acid modifies level-specific cell interactions responsible for intercalary regeneration in axolotl limbs. (Midwest Regional Society for Developmental Biology, Bloomington, IN). Chao, M. and D.L. Stocum 1986 Effects of retinoic acid on the DV axis of regenerating axolotl limbs. (Midwest Regional Society for Developmental Biology, Bloomington, IN). Crawford K. and D.L. Stocum 1986 Retinoic acid modifies level specific cell interactions responsible for intercalary regeneration in axolotl limbs. Am. Zool. 26:60A. (American Society of Zoologists, Nashville, IN). McCormick, A.M., H.E. Shubeita, and D.L. Stocum 1987 Detection and quantitation of retinoic acid binding protein in regenerating axolotl limbs. Anat. Rec. (American Association of Anatomists, Washington, DC). Ludolph, D.C., J.A. Cameron, and D.L. Stocum 1990 Retinoic acid ventralizes positional memory in the dorsoventral axis of regenerating axolotl limbs. Anat. Rec. (American Association of Anatomists, Philadelphia, PA). Ludolph, D.C., R. Gonzales, J.A., Cameron and D.L. Stocum 1991 Test of Posteriorization ad ventralization in retinoic acid treated half limbs of regenerating axolotls. (Midwest Regionals Society of Developmental Biology, West Lafayette, IN).

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Uphues, M., J.A. Cameron and D.L. Stocum 1991 Dose-response of anteroposterior positional identity to retinoic acid in regenerating axolotl limbs. (Midwest Regional Society for Developmental Biology, West Lafayette, IN). Monkemeyer, J., J.A. Cameron, D.L. Stocum 1991 Dose-response of anteroposterior positional identity to retinoic acid in regenerating axolotl limbs. (Midwest Regional Society for Developmental Biology, West Lafayette, IN). Ludolph, D.C. and J.A. Cameron, G. Cox, A. Neff, and D.L. Stocum 1992 Isolation of cDNA clones for axolotl and Xenopus cellular retinoic acid binding protein. (Midwest Regional Society for Developmental Biology, Dayton, OH). Sato, K., Nguyen, T., Smith, R., Chernoff, E., Neff, A., Mescher, A., and Stocum, DL 2000 Subtractive cloning of genes active at regenerative and non-regenerative stages of Xenopis laevis. Midwest Regional Society for Developmental Biology, Urbana, IL Stocum DL and F Song 2007 An experimental approach to the analysis of proximodistal regeneration in regenerating amphibian limbs. Experimental Biology, Washington DC. Nye H, Milner D, Feng L, Li B, Rao N, Redwood P, Miller P, Miller P, Ringwald K, Saulog A, Wilson S, Stocum DL, Wheeler M, Jasiuk I, Cameron JA. (2008) Bioreactor-Based Experiments in Regeneration Biology. 47th Midwest Regional Developmental Biology Meeting, University of Iowa, Iowa City, IA Nye H, Milner D, Li B, Rao N, Miller P, Redwood P, Ringwald K, Stocum DL, Cameron JA (2008) Improving Xenopus laevis Hind Limb Regeneration with Sonic Hedgehog and Fibroblast Growth Factor 10. 47th Midwest Regional Developmental Biology Meeting, University of Iowa, Iowa City, IA Li B, Rao N, Robling AG, Song F, Stocum DL (2008) Axolotl Limb Regeneration: Implications for Bone Regeneration Therapies. First Midwest Stem Cell Biology Conference, Rochester, MI. Li B, Rao N, Robling A, Song F, Stocum DL (2008) Morphological Study of Retinoic Acid-Induced Malformation of Skeletal Tissues during Axolotl Limb Regeneration, Sun Valley Workshop on Skeletal Tissue Biology, ID Rao N, Jhamb D, Milner DJ, Li B, Wang M, Palakal M, King MW, Saranjami B, Nye HLD, Cameron JAC, Stocum DL (2009) Proteomic analysis of blastema formation in regenerating axolotl limbs. Stem Cell Biology Conference, Cold Spring Harbor, NY Nelson M, Keith J, Li B, Stocum D, Li J (2010) Electrospun Nanofibrous Composite Scaffolds for Improved Stem Cell Growth and Proliferation. Orthopaedic Research Society, New Orleans, LA

INVITED PROFESSIONAL PRESENTATIONS Symposia and Workshops British Society for Developmental Biology: Limb and Somite Development, Glasgow, Scotland, 1976.

13
American Society of Zoologists/Canadian Society of Zoologists: Neurotrophic and Morphogenetic Controls in Regeneration, Toronto, Canada, 1977. American Association of Anatomists: Pattern Formation and Morphogenesis, Miami, Florida, 1979. Second Biennial Forum on Regeneration: Ann Arbor, Michigan, 1979. Symposium on Morphogenesis and Pattern Formation: Ann Arbor, Michigan, 1980. British Society for Developmental Biology: Morphogenesis and Pattern Formation, Sussex, England, 1981. Third International Conference on Limb Development and Regeneration: Storrs, Connecticut, 1982. Veterans Administration Symposium: Regeneration Research: Harper's Ferry, Virginia, 1982. NASA Workshop on Developmental Biology: Washington, DC, 1984. NSF Workshop: Future Directions in Regeneration Research: Santa Cruz, California, 1985. Mid-Atlantic Developmental Biology Symposium: Bethesda, Maryland, 1985 (Keynote Speaker). Midwest Regional Developmental Biology Symposium: Cleveland, Ohio, 1985. Canadian Federation of Biological Societies: The Developmental and Cell Biology of Vertebrate Limb Regeneration, Guelph, Canada, 1986. American Society of Zoologists: New Approaches to the Cell Biology of Limb Regeneration, Nashville, Tennessee, 1986. Singer Society Symposium: Regeneration, Chicago, Illinois, 1988 (Keynote Speaker). NATO Advanced Research Workshop: Recent Trends in Regeneration Research, Athens, Greece, 1988 (Plenary Speaker). American Association of Anatomists: Limb Development and Regeneration, New Orleans, Louisiana, 1989. Midwest Regional Developmental Biology Symposium: West Lafayette, Indiana, 1990 (Keynote Speaker). Mini-Symposium on Advances in Limb Development and Regeneration: University of Wisconsin, Madison, Wisconsin, 1991. Singer Society Regeneration Symposium: University of Waterloo, Ontario, Canada, 1992. Orthopaedic Research Society/POSNA Symposium: New Insights Into The Growth Plate, New Orleans, Louisiana, 1994.

14
Japanese Society for Developmental Biology: Tohoku University, Sendai, Japan, 1994. Public Lecture to the City of Sendai: "Prospects for the Regeneration of Human Tissues and Organs: Natural and Artificial Tissue Engineering", Sendai, Japan, 1994. FASEB Summer Research Conference: Wound Healing and Regeneration: At the Interface, Vermont Academy, Saxton's River, Vermont, 1994. Singer Society Symposium: Development and Regeneration, University of Connecticut, Storrs, Connecticut, 1994 (Keynote Speaker). American Society of Zoologists Symposium: The Cutting Edge: Biology of Wound Healing and Regeneration, St. Louis, Missouri, 1995. Orthopaedic Research Society Symposium: Biology of the Growth Plate, Orlando, Florida, 1995. International Symposium on Wound Healing and Regeneration: Hiroshima, Japan, 1995. Keystone Symposium on Molecular and Cellular Biology: Wound Healing in Context, Taos, NM, 1996. Minnesota Academy of Science: Minneapolis, Minnesota, 1997 (Keynote Speaker). Japanese Society for Organogenesis and Tissue Engineering: Osaka, Japan, 1997. Orthopaedic Research Society Symposium: New Orleans, Louisiana, 1998. Japan Research & Technology Corporation Symposium: Stem Cells and Rejuvenation of Life, Oiso, Japan, 2000. Biotechnology: Breakthroughs for Better Living (Alumni Association Frontiers in Science Series, School of Science, IUPUI), Indianapolis, IN, 2001 American Society for Investigative Pathobiology, Stem Cell Workshop, Orlando, FL, 2001 Midwest Developmental Biology Meeting, Columbia, MO, 2001 (Keynote speaker). Hard Tissue Conference, Sun Valley, Idaho, 2001 Banbury Center Symposium, Stability and Reversal of the Differentiated State, Cold Spring Harbor, NY, 2001. Third International Workshop on Musculoskeletal and Neuronal Interactions, Corfu, Greece, 2002 American Society for Investigative Pathology, Workshop on Multilineage Stem Cells, San Diego, CA, 2003 DARPA Regenesis Workshop, San Diego, CA, March 2003 Public Lecture, Biocomplexity III Workshop, Regenerative Biology and Medicine, Bloomington IN, May 2003

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Plenary Lecture, Honors College Undergraduate Research Symposium, Central Missouri State University The Legacy of Frankenstein, Warrensburg, MO, March 2004 DARPA Workshop Accelerated Injury Repair, Washington DC, April 2004 Plenary Lecture, National Council for Undergraduate Research Annual Symposium, IUPUI, The Legacy of Frankenstein, Indianapolis, IN, April 2004 Plenary Lecture, Terre Haute Center for Medical Education, Establishing Successful Research Collaborations, Terre Haute, IN, May 2004 BIO 2004, Panel Chair, Regenerative Biology and Medicine, San Francisco, CA, June 2004 REGENERATE Symposium, Developing a Regenerative Medicine: The Regenerating Amphibian Limb as an Experimental System, Seattle, WA, June 2004 Euroconference: Cellular and Molecular Basis of Regeneration, The Amphibian Limb Regeneration Blastema: A Self-organizing System, San Feliu de Guixols, Spain, September 2004. Enplas Science and Society Lecture, The Legacy of Frankenstein, Biotechnology in the Modern Age , Kennesaw State University, Kennesaw, GA, April 2005. Plenary Lecture, 15th Annual Symposium of the Wound Healing Society, A Call to Amphibian Arms: Weapons for Mammalian Regeneration, Chicago, IL, May 2005. INTEL International Science Fair Workshop Lecture, The Legacy of Frankenstein: Regenerative Biology and Medicine, Indianapolis, IN, May 2006. Biocomplexity IV, Institute for Pure and Applied Mathematics Workshop: Systems Biology and Molecular Modeling. Principles of Developmental Biology andSelf-Organization of the Amphibian Limb Regeneration Blastema, University of California Los Angeles, May 2006. Biotechnology Conference, Indiana Vocational and Technical Institute: Regenerative Biology and Medicine: A Millennial Revolution, Bloomington, IN, October, 2006 Winter College, IUPUI: The Legacy of Frankenstein: Regenerative Biology and Medicine. Sanibel Harbour Resort and Spa, Fort Myers, FL, February, 2007. Biocomplexity V, American Physical Society: Regenerative Biology and Medicine: Research Opportunities for Physicists, Engineers and Mathematicians. Denver, CO, March 2007. Indiana State Science Fair, Keynote Speaker: The Legacy of Frankenstein: Regenerative Biology and Medicine. Indianapolis, IN, March 2007. Cuitting Edge Lectures, IUPUI: The Legacy of Frankenstein: Regenerative Biology and Medicine. Indianapolis, IN, October 2007. University of Florida Regeneration Project: Amphibian Limb Regeneration. Amelia Island Plantation, Jacksonville, FL, December 2007.

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Symposium on Biotechnology: Revolutions in Regeneration Science: iPS Cells and Beyond. Hiroshima University, March, 2008. Biocomplexity VII, Out on a Limb, Indiana University Bloomington, October 2009 Biocomplexity VII, Public Lecture: The Legacy of Frankenstein, Indiana University Bloomington, October 2009 Invited Seminars

1968

Department of Biology, Brown University Department of Biology, Emory University Department of Zoology, University of Illinois

1970 1972 1977 1978

Department of Anatomy, School of Medicine, University of Illinois at Chicago Department of Biology, Wittenberg University Department of Anatomy, School of Medicine, University of Wisconsin Department of Anatomy, School of Medicine, University of South Dakota Neurobiology and Behavior Program, University of Illinois

1979

Department of Zoology, Indiana University Department of Biology, Fisk University Department of Biology, Princeton University

1980 1981

Cell Biology Group, Department of Anatomy, School of Medicine, University of Michigan Department of Anatomical Sciences, University of Illinois Department of Cell and Developmental Biology, University of California, Irvine Medical Research Council, Laboratory of Molecular Biology, Cambridge, England

1982

Department of Zoology, Michigan State University (C.S. Thornton Memorial Lecture). Department of Zoology, University of Iowa Department of Biology, University of Illinois at Chicago

1983

1984

Department of Anatomy and Cell Biology, School of Medicine, University of Michigan Department of Anatomy, University of Kentucky

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Department of Zoology, Ohio State University 1985 1986 1987 Department of Zoology, University of Toronto Department of Anatomy, Rush Presbyterian-St. Lukes Medical Center Department of Molecular Biology, University of Texas at Dallas Department of Anatomy, School of Medicine, University of New Mexico Department of Zoology, Michigan State University Department of Zoology, University of Maryland, Department of Zoology, University of New Hampshire Department of Biology, Princeton University 1988 Department of Biology, University of Maryland, Baltimore County Department of Biology, Illinois Wesleyan University Department of Biology, Miami University of Ohio 1989 Department of Biochemistry, Indiana University School of Medicine School of Dental Medicine, Indiana University 1990 1992 1993 Department of Biology, DePauw University Department of Surgery, University of Minnesota Medical Sciences Program, Indiana University, Bloomington Department of Biology, Indiana University, Department of Biology, Tohoku University, Sendai, Japan Department of Biology, Sogang University, Seoul, Korea Department of Biology, Seoul National University, Seoul, Korea College of Medicine, Seoul National University, Seoul, Korea Yoshizato MorphoMatrix Project, Tsukuba, Japan Developmental Biology Program, Children's Research Foundation, University of Cincinnati 1994 Gerontology Group, Indiana University School of Medicine Department of Anatomy, Indiana University School of Medicine 1995 Department of Foods and Nutrition, Purdue University, West Lafayette

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Department of Cellular Biology, University of Georgia, Athens 1996 Department of Biology, Yamagata City University, Japan Department of Biology, Tohoku University, Sendai, Japan Department of Physics, Indiana University-Purdue University, Indianapolis 1997 Faculty of Science, Hiroshima University, Hiroshima, Japan Department of Biology, Sogang University, Seoul, Korea 1999 Regenerative Biology and Technology Project, Hiroshima University, Japan Department of Biology, Indiana University-Purdue University, Indianapolis Department of Physics, Indiana University-Purdue University, Indianapolis Division of Bioengineering, University of Utah, Salt Lake City 2001 2002 Endocrinology Division, School of Medicine, IUPUI Basic Medical Sciences, School of Veterinary Medicine, Purdue University, West Lafayette Department of Computer and Information Science, IUPUI Department of Biology, Florida Atlantic University, Boca Raton 2003 2004 2005 2006 Developmental Biology Group, University College and Kings College, London Department of Chemistry, University of Southern Indiana Bioethics Research Rounds, Indiana University Center for Bioethics Department of Physics, Indiana University, Bloomington IN: Self-Organization of the Amphibian Limb Regeneration Blastema: Cellular and Molecular Mechanisms. October, 2006 University of Illinois Urbana-Champaign: The Legacy of Frankenstein: Regenerative Biology and Medicine. Urbana, IL, April 2007 Cook Executive Group, Bloomington, IN: Indiana University Center for Regenerative Biology and Medicine. October, 2007 St. Marys College of Maryland: The Legacy of Frankenstein: Regenerative Biology and Medicine. St. Marys City, MD, November 2007. Coe College: Road to Discovery: Self-Organization of the Amphibian Limb Regeneration Blastema. Cedar Rapids, IA, November 2007. Coe College: The Legacy of Frankenstein: Regenerative Biology and Medicine. Cedar Rapids, IA, November 2007.

2007

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Department of Medical and Molecular Genetics, School of Medicine, IUPUI: Mechanisms of Limb Regeneration: Self Organization of the Regeneration Blastema. Indianapolis, IN, December 2007 2008 Public Lecture, Hiroshima, Japan: What are iPS Cells? March, 2008 Amphibian Laboratory, Hiroshima University, Japan: Self-Organization of the Amphibian Limb Regeneration Blastema. March, 2008 University of Illinois Urbana-Champaign: The Legacy of Frankenstein: Regenerative Biology and Medicine. Urbana, IL, April 2008 Jacobs University, Bremen, Germany: The Legacy of Frankenstein: Regenerative Biology and Medicine, May 2008. Indiana State University: The Legacy of Frankenstein: Regenerative Biology and Medicine, September, 2008. Virginia Tech University: The Legacy of Frankenstein: Regenerative Biology and Medicine, November, 2008

2009

University of Illinois Urbana-Champaign, Institute for Genomic Biology: From Frankenstein to Four Factors: Regenerative Biology and Medicine. Urbana, IL, April, 2009 Cook Biotech, West Lafayette Regenerative Medicine Today, September 2009. Purdue University, Department of Animal Science, From Frankenstein to Four Factors: Regenerative Biology and Medicine, October 2009 Indiana University-Purdue University Indianapolis, School of Dentistry, Toward the Chemical Induction of Regeneration in vivo: Amphibians as Research Models, November 2009 Indiana University Bloomington, Department of Physics, Toward the Chemical Induction of Regeneration in vivo: Amphibians as Research Models, November 2009 Indiana University-Purdue University Indianapolis, Scholarship at Lunchtime Series, The Legacy of Frankenstein: Regenerative Biology and Medicine

2010

Central Michigan University, Department of Chemistry, From Frankenstein to Four Factors: Regenerative Biology and Medicine., April 2010. University of Illinois Urbana-Champaign, Institute for Genomic Biology, From Frankenstein to Four Factors: Regenerative Biology and Medicine, April 2010 Indiana University-Purdue University Indianapolis, Department of Biology, A Proteomic Analysis of Blastema Formation in Regenerating Axolotl Limbs, May, 2010. Northeastern University, Department of Biology, A Proteomic Analysis of Blastema Formation in Regenerating Axolotl Limbs, October 2010.

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Northeastern University, Public Lecture, From Frankenstein to Four Factors: Regenerative Biology and Medicine, October, 2010.

SCIENTIFIC PRESENTATIONS TO CIVIC ORGANIZATIONS 1996 1998 Scientech Club of Indianapolis, IN Kiwanis Club, Champaign, IL North Indianapolis Sertoma Club, IN Rotary Club of Indianapolis, IN 2002 Rotary Club, Carmel, IN Retired Doctors Society 2003 2005 Indiana Collaborative Research Breakfast Series Purdue Alumni of Indianapolis Mini Medical School, IUSOM Evolution Discussion Panel, Inherit the Wind, Indiana Repertory Theatre, Indianapolis, IN 2006 The Realms of Science, Symposium on Science and Faith, IUPUI, Indianapolis, IN ICLU Debate, Evolution vs. Intelligent Design, Indianapolis, IN 2009 Regenerative Biology and Medicine, Broad Ripple American Legion Post, Indianapolis, IN 2010 Osher Life Long Learning Institute (OLLI), Champaign, IL

CONFERENCES, SYMPOSIA AND WORKSHOPS ORGANIZED First Biennial Forum on Regeneration: Purdue University, 1977 (Co-Organizer). Fourth Biennial Forum on Regeneration, University of Illinois: 1983 (Co-Organizer). American Society of Zoologists Special Session: "New Approaches to the Cell Biology of Limb Regeneration", Nashville, Tennessee, 1986 (Organizer). 28th Midwest Regional Developmental Biology Meeting: University of Illinois: 1988 (Co-Organizer).

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American Society of Zoologists: "The Cellular and Molecular Basis of Pattern Formation", San Francisco, California, 1988 (Organizer). 29th Midwest Regional Developmental Biology Meeting and 9th Biennial Regeneration Forum: University of Illinois, 1989 (Co-Organizer). Fourth International Conference on Limb Development and Regeneration: Pacific Grove, California, 1992 (Co-Organizer). International Workshop on the Molecular Biology of Axolotls and Other Urodeles: Indiana University-Purdue University, Indianapolis, Indiana, 1993 (Co-Organizer). International Symposium on Wound Healing and Regeneration: Hiroshima, Japan, 1995 (Co-Organizer). 37th Midwest Regional Developmental Biology Meeting and Singer Symposium: Indianapolis, Indiana, 1997 (Co-Organizer). International Symposium: Regeneration and Metamorphosis: Keys to Tissue Restoration, Indiana University-Purdue University, Indianapolis, 1997 (Organizer). 38th Midwest Regional Developmental Biology Meeting: Indianapolis, Indiana, 1998 (CoOrganizer) American Society for Investigative Pathology, Workshop: Multilineage Stem Cells, San Diego, CA, 2003 Institute for Biocomplexity: Regenerative Biology and Medicine, Indiana University Bloomington, 2004, Co-organizer.

REVIEWER SERVICE Reviews of manuscripts for Science, Cell, Developmental Biology, International Journal of Developmental Biology, Journal of Experimental Biology, Development, Developmental Dynamics, Anatomical Record, Phvsiological Zoology, Roux's Archiv, Differentiation, Journal of Experimental Zoology, Wound Repair and Regeneration, Teratology, Cardiac and Vascular Regeneration. Reviews of grant proposals for the National Science Foundation, National Institutes of Health, Veterans Administration, National Research Council of Canada, Wellcome Trust, National Research Council, March of Dimes, NASA, and various private foundations.

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SERVICE ON SCIENTIFIC PANELS AND BOARDS Editorial Boards Regenerative Medicine, 2000-2003 Cardiac and Vascular Regeneration, 2000-2002. Wound Repair and Regeneration, 2003Development, Growth, Differentiation, 2005National Science Foundation Developmental Biology Program, 1985-86. Instrumentation and Laboratory Improvement Program, 1989, 1991. Faculty Awards for Women, 1990. National Institutes of Health Site Visit Panel Member: 1985, 1990, 1991. NICHD, Panel member for RFA on Limb Development and Regeneration, 1985. Special Reviewer, Cell Biology Study Section, NICHD, 1986. Special Reviewer, Molecular Cytology Study Section, 1991. Special Reviewer, 2005 Other Service Veterans Administration Regeneration Research Advisory Board, 1985-1990. Board of Directors, Indianapolis Corporation for Advanced Research, 1989-2004. Chair, Axolotl Colony Advisory Committee, Indiana University, 1996-2004. Finance Committee, American Association of Anatomists, 2002-2005. Mentor for three-student team from Maaynot Yeshiva High School, Teaneck NJ entered in the NSTA/Toshiba ExploraVision National Contest, February-June, 2010. Their project focused on theoretical ways to regenerate a human limb. The team took second place in the national finals for grades 7-9. Consultant to two local high school teams entered in science contests, October 2010. Subjects: limb regeneration, and nanobot eradication of cancer cells.

RESEARCH GRANTS AWARDED Society of Sigma Xi Grant-in-Aid, $250, 1969-1970.

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NSF Grant BMS 71-015 79 A01, $44,900, 1972-1977. NIH Grant HD 8057, $320,552 direct costs, 1973-1978 (Co-PI with H.E. Conrad)) NIH Grant HD 12659, $80,000 direct costs, 1979-1982. NIH Grant HD 12659, $171,000 direct costs, 1982-1985. NIH Grant HD 12659, $239,250 direct costs, 1986-1990. Research Contract: Limb and Spinal Cord Regeneration, Eli Lilly and Company, $277,126, 19992001 Indiana 21 Century Research & Technology Fund, $939,338, 2001-2003. National Science Foundation, $600,000, Co-PI with Ellen Chernoff, 2001-2004. DARPA SBIR II, $200,000, 2004-2005 (subcontract with Robert Fitzsimmons). Indiana 21st Century Research and Technology Fund, $1,680,884, 2004-2006. Keck Foundation, $1, 600, 000, 2006-2009. Army Research Office, $395,000, 2007-2010 RSFG (IUPUI), Co-Investigator with Fengyu Song, Qualitative and Quantitative Study of Repair in Segmental Defects of the Limb and Mandibular Bones of the Axolotl, $22,800, 2010-2011. U.S. Army medical Research & Materiel Command, Telemedicine Advanced Technology Research Center. Special Congressional Appropriation, $1,000,000, 2011-2014. Proposal in preparation for validation. RESEARCH STUDENTS and SCIENTISTS Research Scientists James B. Nardi 1982-1983 "Surface properties of regenerating limb cells" Fengyu Song 2006-2007 Distal Boundary Formation in Regenerating Amphibian Limbs (Currently Assistant Professor in the Indiana University School of Dentistry) Nandini Rao 2007-2010 Proteomic Studies of Limb Regeneration in Ambystoma and Xenopus Bingbing Li 2007-2009 Restoration of Regenerative Capacity in Xenopus Froglet Limbs Xiaoping Chen 2010-2014: Segment Defect Regeneration in the Extremities of Urodele Amphibians
st

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Ph.D. Students Morzlock, Frank V., Ph.D., 1971. "Neural control of RNA synthesis in regenerating limbs of the adult newt". Dearlove, George E., Ph.D., 1973. "Neural control of protein synthesis during amphibian limb regeneration". Keller, Raymond E., Ph.D., 1975. "Vital dye mapping of the gastrula and neurula of Xenopus laevis". Pescitelli, Maurice J., Ph.D., 1980. "Intercalary regeneration in larval Ambystoma limbs: The origin of skeletal structures and the non-segmental organization of morphogenetic information". Kim, Won-Sun, Ph.D., 1985. "The use of retinoids for analysis of positional value in regenerating urodele limbs". Crawford, Karen, Ph.D., 1987. "The use of retinoids and in vivo "affinophoresis" assay to analyze the cellular basis of positional memory in regenerating axolotl limbs" Ludolph, D.C., Ph.D. 1992. "A model for the effects of retinoic acid on positional identity in the transverse axes of the regenerating axolotl limb" (Co-advisor with J.A. Cameron). Paul Miller (Current) Role of Evi 5 in Mitosis During Blastema Formaton in Regenerating Axolotl Limbs. Master of Science Students Powers, Eula, M.S., 1969 (non-thesis degree). Kuntz, Raymond L., M.S., 1973. "Regulation in shift-level transplants of amphibian limb regeneration blastemas". Alex, Anne E., M.S., 1982. "Skeletal regeneration in Ambystoma mexicanum after removal of the anterior or posterior half of the forelimb and hindlimb zeugopodium". Sieben, Louise, M.S., 1984. "Brachial spinal ganglion response to peripheral overload in the larval axolotl, Ambystoma mexicanum". Chao, Mark, M.S., 1987. The effects of retinoic acid on regenerating double dorsal and ventral axolotl limbs. Bergstrom, David, M.S. 1990. "Isolation and characterization of a putative axolotl cDNA of cellular retinoid acid binding protein". Behnaz Mahmoudi (current) VEGF Expression in Regeneration-Competent vs. RegenerationDeficient Amphibian Limbs Undergraduate Research Students I have directed the research of ~60 undergraduate students from 1968-present. Many of these students have become physicians, and several, such as Douglas Melton, Harvard University,

25
Thomas Poole, Syracuse University, and George Guidice, University of Wisconsin Medical College have gone on to outstanding research careers.

TEACHING AND SERVICE ACTIVITIES Undergraduate Courses Taught At University of Illinois Developmental Biology Evolution Comparative Anatomy Cells and Tissues Histology The Organism (Honors Biology Program) At IUPUI Cell Biology Developmental Biology Graduate Courses Taught At University of Illinois Analysis of Development Experimental Development Developmental Biology Seminar Developmental Morphogenesis Seminar At IUPUI Regenerative Biology and Medicine Committee Service Throughout my career, I have served as a member or chairman of numerous committees at the departmental, school and university levels, including search and screen committees for faculty and administrative hires. Other Service I have served as a judge at numerous science fairs at the grade school, high school, local, national, and international level, and have served as mentor for several high school students locally and nationally doing projects on regeneration.

Xianzhong Wang

2011

Curriculum Vitae

XIANZHONG WANG, Ph.D.

Associate Professor Indiana University-Purdue University Indianapolis Department of Biology 723 West Michigan Street, Indianapolis, Indiana 46202-5132 Phone: (317) 278-5714. Fax: (317) 274-2846. Email: xzwang@iupui.edu

Education B.S. 1986. Zhejiang University (formerly Zhejiang Agricultural University), China. Soil and Environmental Sciences. M.S. 1989. Institute of Soil Science, Academia Sinica. Soil and Environ. Sci. Ph.D. 1999. The Ohio State University. Plant Physiological Ecology. Postdoctoral Research 1999-2001. Earth Institute, Columbia University, Postdoctoral Scientist. Academic Appointments 07/2008-present 08/2001-07/2008 08/2001-present 01/2004-present Associate Professor, Department of Biology, IUPUI. Assistant Professor, Department of Biology, IUPUI. Faculty, Center for Earth and Environmental Science, IUPUI. Adjunct Faculty, Department of Earth Science, IUPUI.

Professional Organizations 1997-present. 2003-present. 2004-present. 2003-present. Member, Ecological Society of America. Member, Indiana Academy of Sciences. Member, Botanical Society of America. Member, Hoosier Association of Science Teachers.

Honors and Awards 1999-2001. Postdoctoral Fellowship, Columbia University. 1997-1998. Muellhaupt Presidential Fellowship, The Ohio State University. 1995, 1996 & 1997. Summer Research Awards, University of Michigan Biological Station. 1994-1995. University Fellowship, The Ohio State University.

Xianzhong Wang

2011

Manuscript Review (since 08/2001) American Journal of Botany (1); Bioenergy (1); Biological Invasions (1); Bioresource Technology (2); Chemosphere (1); Conservation Biology (1); Ecology Letters (1); Environmental and Experimental Botany (5); Environmental Pollution (1); Global Change Biology (1); Functional Plant Biology (1); Journal of Environmental Quality (1); Journal of Integrative Plant (1); Journal of Plant Ecology (6); Journal of Plant Physiology (1); New Phytologist (16); Oecologia (2); Plant and Soil (5); Plant, Cell and Environment (1); Restoration Ecology (1); Science of Total Environment (1); Tree Physiology (4); Zhejiang University Science (5). Recent Grants Indiana Space Grant Consortium. 2008-2009. $9,510 IUPUI Office for professional Development. 2004. $3,000. Grant-In-Aid for Research. Purdue Research Foundation. 2003. $7,000. Summer Research Fellowship. Indiana University-Purdue University Indianapolis. 2002, $2,000. Minority Faculty Development Grant. Effects of elevated atmospheric carbon dioxide on plant respiration. Indiana University-Purdue University Indianapolis. 2002, $6,000. Summer Research Fellowship. Effects of elevated atmospheric CO2 concentration on energy plants. Center for Invasive Plant Management, Bureau of Land Management through Montana State University. 07/2007 09/2008, $4,950. Invited Presentations Purdue University Calumet, September 09, 2008. Effects of elevated CO2 on plants: From sex ratios to biomass production. Ningxia Forestry Institute, Yinchuan, China. October 14, 2008. Biomass fuel and global environmental change. Botanical Garden, Academia Sinica, Beijing, China. October 17, 2008. Global Change and Plant Biomass production. Institute of Botany, Chinese Academy of Sciences. March 16, 2005. Effects of higher CO2 on plant physiological ecology. Marian College, Department of Biology, September 15, 2003. Sex-specific responses of male and female plants to CO2 enrichment in the atmosphere. Indiana University Bloomington, Department of Geography. January 24, 2003. Plant respiration in a higher CO2 environment. Kellogg Biological Station, Michigan State University, November 15, 2002. Genderspecific responses to elevated atmospheric CO2 by dioecious plant species.

Xianzhong Wang

2011

IUPUI, Department of Geology. November 16, 2001. Plant respiration in a higher CO2 environment. Selected Peer-Reviewed Journal Articles Fang W and Wang XZ. Impact of invasion of Acer platanoides on canopy structure and understory seedling growth in a hardwood forest in North America. Trees Structure and Function (In Press). Biswas DK, Xu H, Yang JC, LI YG, Chen SB, Jiang CD, Li WD, Ma KP, Adhikary SK, Wang XZ, Jiang GM. 2009. Impacts of methods and sites of plant breeding on ozone sensitivity in winter wheat cultivars. Agriculture, Ecosystems and Environment 134: 168-177. McPeek TM, Wang XZ and Filippelli GM. 2008. Rates of carbon ingrowth and nutrient release from young Icelandic basalts. Jokull 57: 37-44. Taub DR and Wang XZ. 2008. Why are Nitrogen Concentrations in Plant Tissues Lower under Elevated CO2? A Critical Examination of the Hypotheses. Journal of integrative Plant Biology 50: 1365-1374. Biswas DK, Xu H, Li YG, Sun JZ, Wang XZ, Han XG and Jiang GM. 2008. Genotypic differences in leaf biochemical, physiological and growth responses to ozone in 20 winter wheat cultivars released over the past 60 years. Global Change Biology 14: 4659. Wang XZ and Mohan JE. 2008. Effects of global environmental changes on weeds. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources. 2008, 3, No. 067. Ainsworth, E. A., M. R. Rosenberg, and X. Z. Wang*. 2007. Meta-analysis: the past, present and future. New Phytologist 176: 742-744. (Invited meeting report. *Author for correspondence). McPeek, T. M., X. Z. Wang* and G. M. Filippelli. 2007. Rates of carbon ingrowth and nutrient release from young Icelandic basalts. Jokull (Accepted pending revision. Revised manuscript submitted. *Author for correspondence). Biswas, D. K., H. Xu, Y. G. Li, J. Z. Sun, X. Z. Wang, X. G. Han, and G. M. Jiang. 2007. Genotypic differences in leaf biochemical, physiological and growth responses to ozone in 20 winter wheat cultivars released over the past 60 years. Global Change Biology (Accepted pending revision. Revised manuscript submitted). Wang, X. Z. 2007. Effects of species richness and elevated carbon dioxide on biomass accumulation: A synthesis using meta-analysis. Oecologia 152: 595-605. Wang, X. Z. 2007. Gender-specific flowering responses to day length in the dioecious plant Silene latifolia at different temperatures. Sexual Plant Reproduction 20: 45-50. McPeek, T. M. and X. Z. Wang*. 2007. Reproduction of dandelion (Taraxacum officinale) in a higher CO2 environment. Weed Science 55: 334-340. (*Author for correspondence).

Xianzhong Wang

2011

Jiang, C. D., G. M. Jiang, X. Z. Wang, L. H. Li, Y.G. Li, and L. M.Gao 2006. Increased photosynthetic activities and photosystem II thermostability with leaf development in elm (Ulmus punila) seedlings probed by fast fluorescence rise OJIP. Environmental and Experimental Botany 58: 261-268. Wang, X. Z. 2005. Reproduction and progeny of Silene latifolia (Caryophylaceae) as affected by atmospheric CO2 concentration. American Journal of Botany 92(5): 826832. Nagel, J. M., X. Z. Wang, J.D. Lewis,, H. A. Fung, K. L. Griffin and D. T. Tissue. Atmospheric CO2 enrichment alters energy assimilation, investment and allocation in Xanthium strumarium. New Phytologist 166: 513-523. Wang, X. Z., O. R. Anderson and K. L. Griffin. 2004. Chloroplast numbers, mitochondrion numbers and carbon assimilation physiology of Nicotiana sylvestris as affected by CO2 concentration. Environmental and Experimental Botany 51: 21-31. Lewis, J. D., X. Z. Wang, K. L. Griffin and D. T. Tissue. 2003. Age at flowering differentially affects vegetative and reproductive responses of a determinate annual plant to elevated CO2. Oecologia 135: 194-201. Curtis P. S., L. M. Jablonski and X. Z. Wang. 2003. Assessing elevated CO2 responses using meta-analysis. New Phytologist 160: 6-7. Wang, X. Z. and K. L. Griffin. 2003. Sex-specific physiological and growth responses to elevated atmospheric CO2 in Silene latifolia Poiret. Global Change Biology 9: 612618. Jablonski, L. M., X. Z. Wang and P. S. Curtis. 2002. Plant reproduction under elevated CO2 conditions: A meta-analysis of reports on 79 crop and wild species. New Phytologist 156: 9-26. Wang, X. Z. and P. S. Curtis. 2002. A meta-analytical test of elevated CO2 effects on plant respiration. Plant Ecology 161: 251-261. Wang, X. Z. and P. S. Curtis. 2001. Gender-specific variation of Populus tremuloides in its physiological and growth responses to elevated CO2. New Phytologist 150: 675684. Wang, X. Z., J. D. Lewis, D. T. Tissue, J. R. Seemann and K. L. Griffin. 2001. Effects of elevated CO2 on leaf dark respiration of Xanthium strumarium in light and darkness. Proceedings of the National Academy of Sciences, USA 98: 2479-2484. Wang, X. Z., P. S. Curtis and C. S. Vogel. 2001. Effects of soil fertility and atmospheric CO2 enrichment on leaf, stem and root respiration of Populus tremuloides. Pedosphere 199-208.

JOHN C. WATSON CURRICULUM VITAE 1. Personal Information Campus Address: Department of Biology Indiana UniversityPurdue University Indianapolis 723 West Michigan Street Indianapolis, IN 46202-5132 Telephone: Fax: E-mail: (317) 278-1366 (317) 274-2846 jcwatso@iupui.edu

Education: Ph.D. - Indiana University, Department of Biology, June 1982. Major - Plant Physiology; Minors - Biochemistry, Molecular Biology. B.S. cum laude - Butler University, June 1975. Majors - Botany and Chemistry. Professional Experience: Associate Professor; August 1994 - present. IUPUI, Department of Biology, Indianapolis, IN Assistant Professor; 1986 - 1994. University of Maryland, Department of Botany and Center for Agricultural Biotechnology, College Park, MD Carnegie Postdoctoral Fellow, 1982 - 1986. Carnegie Institution of Washington, Department of Plant Biology, Stanford, CA Fellowships and Awards: Trustees Teaching Award, 2007, IUPUI. IUPUI Honors Program Fellowship (2005 - 2006). Golden Key Honor Society Honorary Member; 2002. Teaching Excellence Recognition Award; 1997; 2000; Department of Biology, IUPUI. Outstanding Service Award; 1994; Mid-Atlantic Plant Molecular Biology Society. Excellence Award for Junior Faculty; 1992; Colleges of Life Sciences and Agriculture, UMCP. Carnegie Postdoctoral Fellowship; 1982 - 1986; Carnegie Institution of Washington. Bayard Floyd Fellowship; 1978 - 1979; Indiana University. Sigma Xi Award; 1975; Butler University. Deans List, 1971 - 1975; Butler University. 2. Research Activities A. Publications Santner, A.A. and Watson, J.C. (2006) The WAG1 and WAG2 protein kinases negatively regulate root waving in Arabidopsis. Plant Journal 45: 752-764. Khanna, R., Santner, A.A., and Watson, J.C. (2006) Activity and photoregulated expression of PsPK3. Plant Science 170: 347-355. Bai, F., Watson, J.C., Walling, J., Weeden, N., Santner, A.A., and DeMason, D. (2005) Molecular characterization and expression of PsPK2, a PINOID-like gene from pea 1

(Pisum sativum). Plant Science 168: 1281-1291. Elliot, R.C., Platten, J.D., Watson, J.C. and Reid, J.B. (2004) Phytochrome regulation of pea phototropin. Journal of Plant Physiology 161: 265-270. Briggs, W.R., Beck, C.F., Cashmore, A.R., Christie, J.M., Hughes, J., Jarillo, J.A., Kagawa, T., Kanegae, H., Liscum, E., Nagatani, A., Okada, K., Salomon, M., Rudiger, W., Sakai, T., Takano, M., Wada, M. and Watson, J.C. (2001) The phototropin family of photoreceptors. Plant Cell 13: 993-7. Watson, J.C. (2000) Light and protein kinases. In: Advances in Botanical Research, Volume 32: Plant Protein Kinases, eds. Kreis, M. and Walker, J.C. (Academic Press, San Diego), pp. 149-184. Watson JC (2000) Discovery of photoregulated gene expression. In: Discoveries in Plant Biology, Volume III, eds. Kung, S.-D. and Yang, S.-F. (World Scientific, Singapore), pp. 371-382. . Khanna, R. , Lin, X., and Watson, J.C. (1999) Photoregulated expression of the PsPK3 and PsPK5 genes in pea seedlings. Plant Molecular Biology 39: 231-242. Watson, J.C., Khanna, R., Mitra, T., Ma, J. and Deitzer, G.F. (1997) Light-regulated protein kinase genes. Focus 19 (supplement): 14-15. Invited Review Article. LaRosa, T.J. and Watson, J.C. (1997) NAP1Ps (Accession No. U81289): a putative nucleosome assembly protein from Pisum sativum L. Plant Physiology 115: 313 LaRosa, T.J. and Watson, J.C. PsRT17-1 (Accession No. U81288): a cDNA from Pisum sativum L. encoding a homolog of Axi1 from tobacco. Plant Physiology 115: 313. LaRosa, T.J. and Watson, J.C. (1997) PsGRBP: a cDNA encoding a glycine-rich RNAbinding protein from Pisum sativum L. (Accession No. U81287). Plant Physiology 114: 1568. Khanna, R. and Watson, J.C. (1997) cDNA sequence of PsPK3 (Accession No. U11553): a protein kinase from Pisum sativum L. Plant Physiology 114: 1569. Lu, B., Villani, P.J., Watson, J.C., DeMason, D.A. and Cooke, T.J. (1996) The control of pinna morphology in wildtype and mutant leaves of the garden pea (Pisum sativum L.) International Journal of Plant Sciences 157: 659-673. Fukasawa-Akada, T., Kung, S.-D. and Watson, J.C. (1996) Phenylalanine ammonialyase gene structure, expression, and evolution in Nicotiana. Plant Molecular Biology 30: 711722. Zhao, Y., Watson, J.C., Kung, S-D. and Bottino, P.B. (1995) Characterization of a cDNA encoding ribosomal protein S16 in rice. Plant Physiology 107: 1471-1472. Zhao, Y., Feng, X-H., Watson, J.C., Kung, S-D. and Bottino, P.B. (1994) Molecular cloning and biochemical characterization of a receptor-like serine/threonine kinase from rice. Plant Molecular Biology 26: 791-803. Yang, Z. and Watson, J.C. (1993) Molecular cloning and characterization of rho, a rasrelated small GTP-binding protein from the garden pea. Proceedings of the National Academy of Sciences USA 90: 8732-8736. Yang, Z., Cramer, C.L. and Watson, J.C. (1993) Protein farnesyltransferase in plants: Molecular cloning and expression of a homolog of the b subunit from the garden pea. Plant Physiology 101: 667-674. Watson, J.C., Lin, X. and Yang, Z. (1993) Photoregulated gene expression in plants. In: Recent Advances in Life Sciences: Proceedings of the Fourteenth International Symposium (Kyungpook National University, Taegu, Korea), pp. 181-187. Lin, X. and Watson, J.C. (1992) cDNA sequence of PsPK5, a protein kinase homolog from Pisum sativum L. Plant Physiology 100: 1072-1074. White, M.J., Fritensky, B.W., Falconet, D., Childs, L.C., Watson, J.C., Alexander, L., Roe, B.A. and Thompson, W.F. (1992) Expression of the chlorophyll a/b-binding 2

protein multigene family in pea (Pisum sativum L.): Evidence for distinct developmental responses. Planta 188: 190-198. Lin, X., Feng, X.-H. and Watson, J.C. (1991) Differential accumulation of transcripts encoding protein kinase homologs in greening pea seedlings. Proceedings of the National Academy of Sciences USA 88: 6951-6955. Lai, S., Watson, J.C., Hanson, N.J. and Sze, H. (1991) Molecular cloning and sequencing of a cDNA encoding the proteolipid subunit of the vacuolar H+-ATPase from a higher plant. Journal of Biological Chemistry 266: 16078-16084. Alexander, L., Falconet, D., Fritensky, B.W., White, M.J., Watson, J.C., Roe, B.A., and Thompson, W.F. (1991) Nucleotide sequence of Cab-8, a new type I gene encoding a chlorophyll a/b-binding protein of LHC II in Pisum. Plant Molecular Biology 17: 523-526. Downie, S.R., Olmstead, R.G., Zurawski, G., Soltis, D.E., Soltis, P.S., Watson, J.C. and Palmer, J.D. (1991) Six independent losses of the chloroplast DNA rpl2 intron in dicotyledons: molecular and phylogenetic implications. Evolution 45: 1245-1259. Watson, J.C. (1989) Photoregulation of gene expression in plants. In: Plant Biotechnology, eds. Kung, S.-D. and Arntzen, C.J. (Butterworths, Boston), pp. 161-205. Invited Review Article. Watson, J.C. and Thompson, W.F. (1988) Restriction endonuclease analysis of purified nuclear DNA from plants. In: Methods for Plant Molecular Biology, eds. Weissbach, A. and Weissbach, H. (Academic Press, Orlando), pp. 57-75. Thompson, W.F., Kaufman, L.S., Horwitz, B.A., Sagar, A.D., Watson, J.C. and Briggs, W.R. (1988) Patterns of phytochrome-induced gene expression in etiolated pea seedlings. In: Biomechanisms Regulating Growth and Development, eds. Steffens, G.L. and Rumsey, T.S. (Kluwer, Boston), pp. 269-284. Thompson, W.F., Flavell, R.B., Watson, J.C. and Kaufman, L.S. (1988) Chromatin structure and expression of plant ribosomal RNA genes. In: The Architecture of Eukaryotic Genes, ed. Kahl, G. (VCH Publishers, NY), pp. 385-396. Watson, J.C., Kaufman, L.S. and Thompson, W.F. (1987) Developmental regulation of cytosine methylation in the nuclear ribosomal RNA genes of Pisum sativum. Journal of Molecular Biology 193: 15-26. Kaufman, L.S., Watson, J.C. and Thompson, W.F. (1987) Light-regulated changes in DNase I hypersensitive sites in the rRNA genes Pisum sativum. Proceedings of the National Academy of Sciences USA 84: 1550-1554. Dobres, M.S., Elliot, R.C., Watson, J.C. and Thompson, W.F. (1987) A phytochrome regulated pea transcript encodes ferredoxin I. Plant Molecular Biology 8: 3-12. Watson, J.C. and Thompson, W.F. (1986) Restriction endonuclease analysis of purified nuclear DNA from plants. Methods in Enzymology 118: 57-75. Thompson, W.F., Kaufman, L.S. and Watson, J.C. (1985) Induction of plant gene expression by light. Bioessays 3: 153-159. Invited Review Article. Kaufman, L.S., Watson, J.C., Briggs, W.R. and Thompson, W.F. (1985) Photoregulation of nuclear encoded transcripts: Blue light regulation of specific transcript abundance. In: Molecular Biology of the Photosynthetic Apparatus, eds. Steinback, K., Arntzen, C.J., Bogorad, L., and Bonitz, S. (Cold Spring Harbor Laboratories, NY), pp. 367-372. Briggs, W.R., Mandoli D.F., Shinkle, J.R., Kaufman, L.S., Watson, J.C. and Thompson, W.F. (1985) Phytochrome regulation at the whole plant, physiological and molecular levels. In: Sensory Perception and Transduction in Aneural Systems, eds. Columbetti, G., Lenci, F., and Song, P.-S. (Plenum Press, NY), pp. 265-280. Palmer, J.D., Osorio, B., Watson, J.C., Edwards, H., Dodd, J. and Thompson, W.F. 3

(1984) Evolutionary aspects of chloroplast genome expression and organization. In: Biosynthesis of the Photosynthetic Apparatus: Molecular Biology, Development and Regulation, UCLA Symposium on Molecular and Cellular Biology, New Series, eds. Thornber, J.P., Staehelin, L.A. and Hallick, R.B. (Liss, New York), pp. 273-283. Watson, J.C., Palmer, J.D. and Thompson, W.F. (1983) Chloroplast genes for components of the translational apparatus. Carnegie Institution of Washington Yearbook 82: 24-26. Watson, J.C. and Surzycki, S.J. (1983) Both the chloroplast and nuclear genomes of Chlamydomonas reinhardi share homology with Escherichia coli genes for transcriptional and translational components. Current Genetics 7: 201-210. Watson, J.C. and Surzycki, S.J. (1982) Extensive sequence homology in the DNA coding for elongation factor Tu from Escherichia coli and the Chlamydomonas reinhardtii chloroplast. Proceedings of the National Academy of Sciences USA 79: 2264-2267. B. Recent Meeting Abstracts Cheek, K., Chawla, R., Santner, A.A., Alzoubi, S., Cheng, Y., Murphy, A.S. and John C. Watson, J.C. (2009) The role of WAG1 and WAG2 in root waving in Arabidopsis. Ninth International Plant Molecular Biology Congress (St. Louis, MO). Watson, J.C., Chawla, R., Alzoubi, S., Kay Cheek, K., Rowland, S., Peer, W.A. and Murphy, A.S. (2009) Molecular analyses of the role of WAG1 and WAG2 in root waving. Ninth International Plant Molecular Biology Congress (St. Louis, MO). C. Research Funding Current Extramural Funding: National Science Foundation (Integrative Plant Biology Program). Protein kinases that regulate root waving. Principal Investigator: J.C. Watson. $350,000 total costs; March 15, 2006 March 14, 2009 (no-cost extension through 2011). Current Intramural Funding: Intercampus Applied Research Program (IUPUI and Purdue University). Regulation of root growth by a soybean protein kinase Principal Investigator: J.C. Watson. Co-Principal Investigator: Angus S. Murphy (Dept. of Horticulture, Purdue Univ.). $50,000 total costs; February 2008 January 2010. IUPUI School of Science. Acquisition of Laser Scanning Confocal Microscope in the Nanoscale Imaging Center Principle Investigator: C. Naumann (Chemistry & Chemical Biology, IUPUI). Co-Principal Investigators: J.C. Watson, C. Goodlett (Psychology), R. Decca (Physics). $215,000. Previous Extramural Funding: USDA National Research Initiative Competitive Grants Program (Plant Growth and Development Panel). Light- and auxin-regulated protein kinases. Principal Investigator: J.C. Watson. $120,000 total costs; Sept. 2002 Aug. 2004 (no-cost extension through Aug. 2006). National Science Foundation (Functional and Regulatory Systems Cluster). Photoregulated protein kinases in plants. 4

Principal Investigator: J.C. Watson. $300,000 total costs; February 1998 January 2003. National Science Foundation (Integrative Plant Biology Program). Photoregulated protein kinases in plants. Principal Investigator: J.C. Watson. $235,000 total costs; April 1992 March 1998. National Science Foundation (Instrumentation Program) A phosphoimager for molecular biology research. Principal Investigator: P.L. Crowell. Co-Principal Investigators: D.N. Crowell, E.C. Long, S.J. Rhodes, J.C. Watson. $52,000 total costs; Sept. 1999 Aug. 2000. DOE/NSF/USDA Triagency Plant Biology Program on Collaborative Research. Research collaboration group in plant protein phosphorylation. Principal Investigator: D.D. Randall. Co-Principal Investigators: T. Bleeker, R. Chollet, T. Guilfoyle, A. Harmon, J. Harper, M. Harrington, S. Huber, M. Jacobs, T. Jacobs, T. Lomax, D. Roberts, S. Roux, M. Sussman, J. Walker, J.C. Watson. $50,000 per year; September 1992 August 1998. USDA Competitive Grants Program (Plant Growth and Development Panel). Photoreceptor interaction in light-regulated gene expression. Principal Investigator: J.C. Watson. $200,000 total costs; September 1989 August 1992 with no-cost extension through August 1993. Shell Agricultural Chemical Co. (Modesto, CA). In vitro transcription systems from plant nuclei. Co-Principal Investigators: J.C. Watson and W.F. Thompson (Carnegie Institution of Washington). $67,000, July 1985 June 1986. Previous IUPUI and Purdue Research Foundation Funding: Faculty Development Grant-In-Aid of Research. Light and auxin-regulated protein kinases. Principle Investigator: J.C. Watson. $3,000; May 2002 April 2005. Faculty Development Grant-In-Aid of Research. Molecular cloning of the photoreceptor for phototropism. Principle Investigator: J.C. Watson. $3,000; Fall 1997. Declined by P.I. Purdue Research Foundation Summer Faculty Grant. Signaling pathways involving photoregulated protein kinases. Principal Investigator: J.C. Watson. $5,000; summer 1997. Faculty Development Summer Research Fellowship. Identification of components of signal transduction pathways involving photoregulated protein kinases. Principal Investigator: J.C. Watson. $6,000; Summer 1996. Faculty Development Grant-In-Aid of Research. Molecular cloning of cyclic nucleotide-binding proteins from plants. Principle Investigator: J.C. Watson. Collaborators: Drs. B. Biermann, D. Crowell, S.R. Randall. 5

$3,000; July 1995 - June 1996. Previous University of Maryland Funding: Maryland Agricultural Experiment Station Competitive Grants Program. Nucleolin gene expression during photoregulated leaf development. Principal Investigator: J.C. Watson. $22,000; July 1993 - June 1994. Maryland Agricultural Experiment Station Competitive Grants Program. Nucleolin gene expression during photoregulated leaf development. Principal Investigator: J.C. Watson. $25,000, July 1992 - June 1993. Maryland Agricultural Experiment Station Competitive Grants Program. Molecular cloning of a protein that is not expressed in a photoperiod insensitive mutant of barley. Co-Principal Investigators: G.F. Deitzer and J.C. Watson. $25,000, July 1992 - June 1993. Maryland Agricultural Experiment Station Competitive Grants Program. Nucleolin gene expression during photoregulated leaf development. Principal Investigator: J.C. Watson. $24,000, July 1991 - June 1992. Maryland Agricultural Experiment Station Competitive Grants Program. Molecular genetics of flowering. Co-Principal Investigators: G.F. Deitzer and J.C. Watson. $27,000; July 1990 - June 1991. Maryland Agricultural Experiment Station Competitive Grants Program Protein kinases in the regulation of plant development. Co-Principal Investigators: J.C. Watson and S.M. Wolniak $30,000; July 1990 - June 1991. Maryland Agricultural Experiment Station Competitive Grants Program. Molecular genetics of flowering in Pisum sativum L. Co-Principal Investigators: G.F. Deitzer and J.C. Watson. $27,175; September 1989 - August 1990. Maryland Agricultural Experiment Station Competitive Grants Program. Signal transduction mechanisms in plants: Protein kinase C as a component in developmental cascades. Co-Principal Investigators: J.C. Watson and S.M. Wolniak. $30,000; September 1989 - August 1990. Biomedical Research Support Award (University of Maryland at College Park). Transcriptional regulation of nuclear gene expression in plants. Principal Investigator: J.C. Watson $9,500; September 1989 - August 1990. Biomedical Research Support Award (University of Maryland at College Park). Multiple interactions in photoregulated gene expression in plants. Principal Investigator: J.C. Watson. $3,200; July 1988 - March 1989. General Research Board (University of Maryland at College Park). In vitro transcription in isolated nuclei from plants. Principal Investigator: J.C. Watson. $1,500; July 1988 - March 1989. Center for Agricultural Biotechnology of the Maryland Biotechnology Institute. 6

Production of transgenic cereals by direct incorporation of plasmid DNA into premeiotic germ cells of developing floral primodia. Co-Principal Investigators: G.F. Deitzer, J.C. Watson, D.J. Sammons & V. Smail. $17,000, December 1987 - November 1988. Maryland Agricultural Experiment Station Projects: Light and hormonal interactions in photoregulated gene expression. Project Number MD-J-142. October 1988 - September 1992. Photoregulated expression of protein kinase and nucleolin gene expression in plants. Project Number MD-J-162. June 1993 - June 199 D. Advising of Research Students Major Advisor Completed Ph.D. Degrees Name Department Year Aaron Santner Biology, IUPUI 2006 Rajnish Khanna Tomoko Fukasawa-Akada Xia Lin Biology, IUPUI Botany, UMCP Botany, UMCP 1998 1996 1993 Dissertation title Light- and auxin-regulated protein kinases from Arabidopsis thaliana Light-regulated protein kinases from plants Organization and expression of phenylalanine-ammonia lyase genes in Nicotiana tabacum Molecular cloning and characterization of transcripts encoding protein kinase homologs from pea (Pisum sativum L.) Thesis title Transgenic expression of WAG1 and WAG2 in Arabidopsis thaliana Dimerization of WAG2 from Arabidopsis thaliana Structure and expression of the pea phototropin 1 gene The PsPK4 gene from the garden pea encodes phototropin 1 Photoregulated protein kinases from the garden pea Identification of nucleolin related sequences in plants

Major Advisor Completed M.S. Degrees Name Department Year Shadia Alzoubi Biology, IUPUI 2008 Kay Sherfick David Wormser Rodney Oakley Aaron Santner Thomas LaRosa Biology, IUPUI Biology, IUPUI Biology, IUPUI Biology, IUPUI Biology, IUPUI 2007 2003 2002 2001 1996

Major Advisor Current Ph.D. Candidates Name Tentative thesis title Kay Sherfick Role of WAG1 and WAG2 in root waving Major Advisor Current M.S. Candidates Name Tentative thesis title Steven Rowland Role of WAG1 and WAG2 in lateral root development

Member of Graduate Committee Completed Ph.D. Degrees (since 1995) Name Department Year Mentor David Huizinga Biology, IUPUI 2009 D.N. Crowell Ann Kimble-Hill Chemistry, IUPUI 2008 C. Naumann Gretchen Parker Biology, IUPUI 2002 S.J. Rhodes Brian Downes Biology, IUPUI 2001 D.N. Crowell Darren Seals Biology, IUPUI 1998 S.K. Randall Member of Graduate Committee Completed M.S. Degrees (since 1995) Name Department Year Mentor Yuji Yamasaki Biology, IUPUI 2007 S.K. Randall Rhonda Thomas Biology, IUPUI 2006 P. Crowell Aaron West Biology, IUPUI 2006 B. Blazer-Yost Michael Anne Shane Biology, IUPUI 2006 B. Blazer-Yost Cynthia Johnson Biology, IUPUI 2005 D.N. Crowell Gage Koehler Biology, IUPUI 2005 S.K. Randall Danielle Christman Biology, IUPUI 2004 S.K. Randall Zhaohong Li Biology, IUPUI 1999 D.N. Crowell Jeffrey Price Biology, IUPUI 1995 S.K. Randall Jin Ma Horticulture., UMCP 1995 G.F. Deitzer Teresa Morehead Biology, IUPUI 1995 D.N. Crowell Member of Graduate Committee Current M.S. and Ph.D. Candidates Name Department Degree sought Mentor Yuji Yamasaki Biology, IUPUI Ph.D. S.K. Randall Gage Koehler Biology, IUPUI Ph.D. S.K. Randall Sreejith Ramakrishnan Biology, IUPUI Ph.D. A. Malkova Mentoring of Postdoctoral Associates Name Year Dr. Rekha Chawla 2006-2008 Dr. S. Narasimha Chary 1998-2001 Dr. Zhenbiao Yang 1990-1993 3. Selected Professional Service Activities A. Memberships in Professional Societies American Society of Plant Biologists American Association for the Advancement of Science Society for Plant Signaling and Behavior B. Ad Hoc Reviewing for Journals Developmental Biology Gene Journal of Biological Chemistry Photochemistry and Photobiology Planta Plant Cell 8 Current Position Staff Scientist, JR Simplot Staff Scientist, Dow Agrosciences Professor, UC Riverside

Plant, Cell, and Environment Plant Physiology Plant Molecular Biology Plant Journal Physiologia Plantarum Proceedings of the National Academy of Sciences U.S.A. Trends in Plant Science C. Reviewing Activities for Federal Agencies: Grant Review Panel Manager: USDA Small Business Innovation Research Program Plant Production and Protection Panel (September 2004 May 2005) Grant Review Panel Memberships: National Science Foundation Integrative Plant Biology Panel (April 2003) Integrative Plant Biology Panel (April 2004) USDA Small Business Innovation Research Program Plant Production and Protection Panel (January 2003) Plant Production and Protection Panel (January 2004) USDA Competitive Grants Program Plant Responses to the Environment Panel (June 1990). Agency for International Development, Program in Science and Technology Cooperation Plant Biotechnology/Genetic Engineering Panel (Oct. 1987). Plant Molecular Biology, Immunology, Microbiology Completed Project Panel (July 1988). Molecular Genetics Panel (Oct. 1989). Ad Hoc Reviewing for Agencies: USDA Small Business Innovation Research Plant Production and Protection Panel USDA National Research Initiative Competitive Grants Program Plant Responses to the Environment Panel Plant Growth and Development Panel Plant Molecular Biology Panel National Science Foundation Integrative Biology Cluster Integrative Plant Biology Program Eukaryotic Genetics Program Physiological Processes Program Instrumentation and Instrument Development Program Physiology and Behavior Program Cellular Biochemistry Program Cell Biology Program Cell Organization Program Molecular Biochemistry Program USDA/ARS Beltsville Agricultural Research Center - Manuscript Peer Review. ARS Research Project Review 9

U.S.-Israel Binational Agricultural Research and Development Fund Cellular and Molecular Biology Panel D. Service for Local Private Entities Dow Agrosciences (Indianapolis, IN), Institutional Biosafety Committee, Member (June 2008present). 4. Selected University Service Activities at IUPUI A. Department of Biology Committee Assignments Committee Position Curriculum Committee Chair Member Planning Committee Member Promotion, Tenure, and Reappointment Member Committee Seminar Committee Cell and Developmental Biology Qualifying Exam Committee Biochemistry and Molecular Biology Qualifying Exam Committee Lecturer Search Committee Ecology Search Committee TERA Nominations Committee Member Member Chair Member Member Member Member Term Fall 2005Spring 2009 Fall 1996Spring 2005 Fall 2005 Fall 2008present Fall 2004Spring 2007 Fall 1999Spring 2001 Fall 2001Fall 2007 Fall 1994Fall 2007 Spring 2008present Fall 1994Fall 2007 Fall 2002Spring 2003 Fall 2000Spring 2001 Spring 1997, Spring 1998 Term Fall 2009present Fall 1998Spring 2000 Fall 2006Fall 2007 Fall 1994Spring 1996 Fall 1995 Term Fall 2009present Fall 2008present Spring 2001present Spring 2004Fall 2007 Fall 1999Fall 2004 Fall 1995

B. School of Science Committee Assignments Committee Position Undergraduate Education Committee Chair Public Relations Committee Member Steering Committee Member Research Committee Member

C. Campus Level Committee Assignments Committee Position Faculty Council Member (At-Large) Undergraduate Curriculum Advisory Member Committee Institutional Biosafety Committee Alternate member Center for Research and Learning Board Member of Directors Undergraduate Research Opportunities Member Steering Committee Resources Taskforce of the College Member Consolidation Planning Committee

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5. Classroom and Individual Instruction A. Classroom Instruction (since 1995) Course Cell Biology Laboratory (Biology K325) Semester Spring 1995 Spring 1996 Spring 1997 Spring 1998 Spring 1999 Spring 2000 Spring 2001 Spring 2002 Spring 2003 Spring 2004 Spring 2005 Spring 2006 Spring 2007 Spring 2009 Spring 2010 Fall 1995 Fall 1996 Fall 1997 Fall 1998 Fall 1999 Fall 2000 Fall 2001 Fall 2002 Fall 2003 Fall 2004 Fall 2005 Fall 2006 Fall 2007 Fall 2008 Fall 2009 Spring 2009 Spring 2010 Year 2000 2001 2002 2003 2004 2005 2006 2007 11 Enrollment 18 23 30 21 57 51 55 63 70 71 79 81 105 134 160 11 17 16 28 33 23 33 25 29 24 47 64 79 88 89 4 3 Enrollment 1 3 2 3 4 1 1 1 Global Score 4.79 4.60 4.39 4.63 4.14 4.25 NA 4.28 4.40 4.17 4.26 4.34 4.40 4.47 TBA 4.41 4.52 4.25 4.35 4.43 4.25 4.52 4.32 4.30 4.62 4.74 4.64 4.75 4.76 4.67 NA NA Total Credits 2 3 3 5 5 5 1 1

Cell Biology (Biology K324)

Plant Molecular Biology (Biology 550)

Plant Molecular Genetics (Biology 697) Plant Omics (Biology 697) B. Individual Instruction (since 2000) Course Independent Research (Biology K493)

Senior Research Thesis (Biology K494)

Capstone (Biology K490)

M.S. Research (Biology 698)

Ph.D. Research (Biology 699)

2008 2001 2002 2003 2004 2005 2006 2007 2008 2009 2000 2001 2002 2003 2005 2006 2007 2008 2009 2003 2004 2005 2006

2 1 1 2 2 1 2 1 1 1 3 3 2 2 1 2 2 3 2 1 1 1 1

2 2 1 2 2 1 2 1 1 1 22 21 16 5 9 30 14 21 24 8 10 12 1

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